D. Wulandari, Giwang Dewantoro, A. T. Lunggani, A. Suprihadi, C. Riani, E. Setiawan, Siti Luthiatul Farikha, A. Budiharjo
{"title":"Antibacterial Activity of Freshwater Sponge Oncosclera asiatica Against Escherichia coli and Staphylococcus aureus","authors":"D. Wulandari, Giwang Dewantoro, A. T. Lunggani, A. Suprihadi, C. Riani, E. Setiawan, Siti Luthiatul Farikha, A. Budiharjo","doi":"10.14710/bioma.24.2.120-129","DOIUrl":"https://doi.org/10.14710/bioma.24.2.120-129","url":null,"abstract":"Freshwater sponges are animals from the Porifera phylum that live in freshwater. The sponge used is Oncosclera asiatica was taken from Kali Porong, East Java. Seventeen isolates of bacteria have been obtained from isolation. Antibacterial potential testing was performed by paper disc inhibition assay using Escherichia coli and Staphylococcus aureus as pathogenic bacteria and amoxicillin as a positive control. The antibacterial activity test showed that four isolates have the potential activity. The isolates with the highest inhibition zones were identified using a 16S rRNA.The results of BLAST showed isolate number 2 was Pseudomonas moraviensis with 99.51% similarity. The phylogenetic tree analysis was build using the MEGA X program. The results of the phylogenetic tree analysis showed that P.moraviensis had a bootstrap value of 100% with a genetic distance value of 0.001. P. moraviensis isolates screened for the presence of Non-Ribosomal Peptide Synthetase (NRPS) gene by A2gamF and A3gamR primers. The amplification result from NRPS gene showed positive meaning that P.moraviensis genome contained NRPS gene.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122039586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ota Yulia Sari, Wahyu Aji Mahardika, Nadhila Mutia Afsari, Ivan Permana Putra
{"title":"Eksplorasi Jamur Desa Tulung Agung, Lampung Timur dan Potensi Pemanfaatannya","authors":"Ota Yulia Sari, Wahyu Aji Mahardika, Nadhila Mutia Afsari, Ivan Permana Putra","doi":"10.14710/bioma.24.2.105-119","DOIUrl":"https://doi.org/10.14710/bioma.24.2.105-119","url":null,"abstract":"Mushrooms an be found in various places, such as litter, rotten wood, trees, and soil around residential areas. Tulung Agung Village in East Lampung Regency has various kinds of vegetation and several other abiotic factors that support the growth of mushroom. The study of mushroom’s exploration could elaborate the knowledge about mushroom diversity in resdential areas. This study aims to explore the types of mushrooms based on the description of their fruiting bodies from the residential location of Tulung Agung Village, District Pekalongan, East Lampung Regency, Lampung. The methods used were purposive sampling technique in a shady area or under the shade of a tree, documenting the mushroom fruiting bodies hat were found, and presenting mushrooms with fruiting bodies characters. After the exploration, 18 species from 14 genera of mushrooms were found, including Auricularia auricula-judae, Collybia sp., Conocybe sp., Daldinia sp., Gymnopilus sp., Marasmius sp., Marasmius haematocephalus, Mycena sp., Lepiota sp., Parasola sp., Pycnoporus sp., Trametes sp., Tremellodendron sp., and Xylaria sp. The various potentials of each type of mushrooms that were found are for food sources, traditional medicines, biodegradation of contaminants, sources of bioluminescence, and sources of various bioactive compounds. The Mushroom that were found need molecular identification to get the correct species so that the potential of Mushroom can be utilized optimally by residents based on more specific type information.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124516609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Respon Histologis Aorta dan Jantung Rattus Norvegicus Hiperlipidemia Setelah Pemberian Jus Buah Kersen (Muntingia calabura L.) dan Ekstrak Daun Lakum (Cayratia trifolia L.)","authors":"E. Agustina, T. Saraswati, Silvana Tana","doi":"10.14710/bioma.24.2.96-104","DOIUrl":"https://doi.org/10.14710/bioma.24.2.96-104","url":null,"abstract":"This study aims to determine the histological features of the aorta and heart of male Rattus norvegicus after administration of cherry juice (Muntingia calabura L.) dan lakum leaf extract (Cayratia trifolia L.). The study used 30 male Rattus norvegicus L. in a Completely Randomized Design (CRD) consisting of 5 treatments with 6 repetitions, namely the control group (P0), the standard feeding group (P1), the hyperlipid feed group + 0.2 mL/200 g BB cherry juice (P2), the hyperlipid feed group + 40 mg / 200 g BB lakum leaf extract (P3) and hyperlipid feed group + 0.18 mg / 200 g BB simvastatin (P4). Hyperlipid feed was given during the treatment, which was 28 days. To obtain histological data, preparations were made using the paraffin method and then observed using a photomicrograph with 400x magnification and observing the aortic lumen, foam cells and intracellular accumulation of lipids in smooth muscle. Data were analyzed by ANOVA followed by Duncan Test at a significant level of 5%. The results showed that the addition of cherry juice and lakum leaf extract gave a significant effect on lumen width and thickness of the aortic wall (p <0.05), while on changes in heart muscle cell size no significant effect (p> 0.05). Histomorphometri showed the presence of foam cells and accumulation of smooth muscle lipids in the control and group treatments with hyperlipid feeding. The conclusion of this study was that the addition of cherry juice and lakum leaf extract can reduce atherosclerotic lesions by eliminating showed foam cells and prevent proliferating smooth muscle cells so the size of the aortic lumen width returns to normal, but didn’t cause enlargement of the heart muscle (cardiomegaly).","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133632229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Kelimpahan, Persebaran Populasi, Preferensi Pakan dan Ketersediaan Tumbuhan Pakan Burung Bondol (Lonchura Spp) di Beberapa Tipe Habitat Kota Semarang Jawa Tengah","authors":"A. Ardianto, Karyadi Baskoro, R. Rahadian","doi":"10.14710/bioma.24.1.54-60","DOIUrl":"https://doi.org/10.14710/bioma.24.1.54-60","url":null,"abstract":"The Semarang City is a metropolitan city with such rapid development, so that it also affects several animals, one of which is birds. One species of bird that can be found in various habitats is the Genus Lonchura. This study aims to examine the abundance and population distribution, feed preferences, and the availability of food plants for munia in urban, suburban, rural habitats in Semarang City. Data were collected using the transect method, Visual Encounter Survey (VES), and quadratic. Analysis of the data used are abundance index, diversity index, equity index, Morisita index, Kruskall-Wallis test, and Chi-Square Test/ Goodness of Fit. Three species of Lonchura, namely Scaly-breasted munia (Lonchura punctulata), White-headed munia (Lonchura maja), and Javan munia (Lonchura leucogastroides), are found in urban, suburban and rural habitats. The Genus Lonchura in urban and rural habitats is dominant, while the distribution of the bondol bird population based on the Morisita Index is clumped. The munias also did not show any food preference for the types of forage plants.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133498558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. T. Lunggani, Wahyu Aji Mahardhika, Aditya Budi Utomo, E. Kusdiyantini
{"title":"Molecular Characterization Of Phylloplane Mold From Avicennia marina Leaves","authors":"A. T. Lunggani, Wahyu Aji Mahardhika, Aditya Budi Utomo, E. Kusdiyantini","doi":"10.14710/bioma.24.1.61-65","DOIUrl":"https://doi.org/10.14710/bioma.24.1.61-65","url":null,"abstract":"Mangroves are a habitat for organisms and microorganisms, including phylloplane molds. Phylloplane molds are known to have various potentials such as antimicrobial, enzyme, and pigment-producing. PFM19 is an orange pigment-producing phylloplane mold. Identification of the mold is needed to determine the species of the fungus so that it can be used for further research. This study aims to identify molecularly the PFM19 mold that produces orange pigment using ITS markers. The methods used in this study included the rejuvenation of isolates, DNA extraction, DNA amplification, and phylogenetic analysis. The results obtained that PFM19 has similarities with Talaromyces islandicus CBS 388.48 by 100% based on ITS markers.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115039431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Asep Komara Walkis Hayadri, H. P. Kusumaningrum, Anton Budiharjo
{"title":"Simulasi Blood Type Inheritance dengan Pemrograman Software Wolfram","authors":"Asep Komara Walkis Hayadri, H. P. Kusumaningrum, Anton Budiharjo","doi":"10.14710/bioma.24.1.66-72","DOIUrl":"https://doi.org/10.14710/bioma.24.1.66-72","url":null,"abstract":" Bioinformatics as an interdisciplinary science, combine biology, computer science, information technology, mathematics, and statistics in order to analyze and interprete biological data. Bioinformatics has been used for in silico analysis of biological questions using mathematical and statistical techniques. In silico analysis allows indirect simulation through a series of instructions put into a device, usually a computer. Nowadays, the simulation of biological aspects, especially genetics, with in silico method has been widely applied for several fields. The ABO blood type inheritance system in humans has rules that its mechanism can be applied into the Wolfram software programming, for example showing the possibility of blood groups being inherited from blood type O pairs with AB. The latest statistical data regarding the number of Indonesians who have reported their blood type has not yet reached 25% of the total population of Indonesia. Wolfram software is expected to be an accurate and fast blood type detector to record the entire population of Indonesia. The purpose of this study was to simulate Blood Type Inheritance with Wolfram Software Programming on ABO blood type inheritance data and see the suitability of the simulation results with real blood type inheritance data. The method used to simulate blood type inheritance is a survey of 12 families for the data of blood group, the next step is to enter the data one by one into the simulation model to observe the output results then matched with real data to determine the results of ABO blood type inheritance. Simulation of blood type inheritance can be done by programming the Wolfram Mathematica software and blood type data from 12 families can be simulated through the ABO blood inheritance system on Wolfram Mathematica, with details of the simulation predicting the blood groups of 2 families, simulation of 9 families according to real data, and simulation of 1 family does not match the real data.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125386258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Struktur Komunitas dan Habitat Odonata di Kawasan Wisata Waduk Jatibarang Semarang","authors":"Megayani Yuditaningtyas, M. Hadi, Udi Tarwotjo","doi":"10.14710/bioma.24.1.73-79","DOIUrl":"https://doi.org/10.14710/bioma.24.1.73-79","url":null,"abstract":"Jatibarang Reservoir is a freshwater dam in Semarang which has different habitat types. The different of habitat types can affect biodiversity, including dragonflies (odonata). Dragonflies are insects that have an important function as a bioindicator of water quality and play a role in maintaining the balances of tropic levels in the food chain. The study aims to determine the abundance, diversity, evenness, similarity and distribution of dragonflies and to determine the abiotic and biotic factors of each habitat type in Jatibarang Reservoir tourist area. The study used Point Count method. The results showed that there were 22 types of dragonflies consisting of 7 different families. The dragonfly which has the highest abundance in the total number of individuals is Eupahea variegata with an index of 16.77%. Diversity of dragonflies in low and medium categories. The evenness level of dragonfly species is evenly. The similarity of species has a low to high degree of similarity. The distribution of dragonflies are clumped and regular. The differences in abiotic and biotic conditions in each habitat affect the structure of dragonfly community.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"176 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124355827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Struktur Komunitas Kupu-Kupu (Lepidoptera: Rhopalocera) di KHDTK Wanadipa Undip Kabupaten Semarang, Jawa Tengah","authors":"Tyas Citra Kusuma, M. Hadi, Jafron Wasiq Hidayat","doi":"10.14710/bioma.24.1.90-95","DOIUrl":"https://doi.org/10.14710/bioma.24.1.90-95","url":null,"abstract":"Kawasan Hutan Dengan Tujuan Khusus (KHDTK) Wanadipa Undip is one part of the Penggaron forest that located in East Ungaran District, Semarang Regency. Part of the KHDTK Wanadipa Undip area has a variety of vegetation that has the potential as a habitat for butterflies. This study aims to analyze the community structure and identify the types of butterflies found in KHDTK Wanadipa Undip, Semarang Regency. Butterflies are one of the herbivores in terrestrial ecosystems that have several roles in the ecological and economic fields. The method used is roaming and point determination using a point count of 4 plots with a distance of 50 meters from each point and a time of ± 20 minutes. The results showed 31 types of butterflies belonging to 5 families. The highest relative abundance index value at 3 stations was found in the species Catopsilia pyranthe, while the lowest was at station 2, namely Euploea mulciber and Hypolimnas bolina species. The diversity index value is 1.43 – 3.12 which is classified as low to moderate. The evenness index value at all stations is classified as almost evenly, which is 0.74 – 0.93. The highest similarity index is found at stations 3 and 4 as well as environmental factor parameters at KHDTK Wanadipa Undip such as altitude, temperature, humidity, wind speed, and light intensity, which are sufficient for butterfly life.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126756520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ilham Aris Wandi, Jonathan Alva Samudra, Roiyan Nur Khoirul Umam, Rina Sari Asih, Mursyidatun Nafiah, Siti Nur Jannah, Rejeki Siti Ferniah
{"title":"Eksplorasi Senyawa Antiretroviral dari Biji Tanaman Calophyllum inophyllum L. sebagai Alternatif Obat AIDS secara In Silico","authors":"Ilham Aris Wandi, Jonathan Alva Samudra, Roiyan Nur Khoirul Umam, Rina Sari Asih, Mursyidatun Nafiah, Siti Nur Jannah, Rejeki Siti Ferniah","doi":"10.14710/bioma.24.1.24-29","DOIUrl":"https://doi.org/10.14710/bioma.24.1.24-29","url":null,"abstract":"Retroviruses are a type of positive-sense RNA virus single-stranded with DNA intermediates and as obligate parasites that target host cells. The principle of DNA replication of retorvirus is not in a regular pattern but in an inverted pattern. Therefore, a study was conducted to find candidates for natural compounds from the seeds of the plant Calophyllum inophyllum L. and found that the most potential as a candidate for AIDS drugs was Calophyllolid. However, further research needs to be done and needs to be considered. This is because the binding affinity number is too small. Based on the Lipinski test, Calophyllolid does not meet all Lipinski's rules so that its use as a drug will be used by injection or other means than oral. In the toxicity test, Calophyllolid has the same value as the other 3 compounds, so a special test is needed to eliminate some of the possible carcinogenicity.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128559517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}