A. T. Lunggani, Wahyu Aji Mahardhika, Aditya Budi Utomo, E. Kusdiyantini
{"title":"海棠叶状面霉菌的分子特性研究","authors":"A. T. Lunggani, Wahyu Aji Mahardhika, Aditya Budi Utomo, E. Kusdiyantini","doi":"10.14710/bioma.24.1.61-65","DOIUrl":null,"url":null,"abstract":"Mangroves are a habitat for organisms and microorganisms, including phylloplane molds. Phylloplane molds are known to have various potentials such as antimicrobial, enzyme, and pigment-producing. PFM19 is an orange pigment-producing phylloplane mold. Identification of the mold is needed to determine the species of the fungus so that it can be used for further research. This study aims to identify molecularly the PFM19 mold that produces orange pigment using ITS markers. The methods used in this study included the rejuvenation of isolates, DNA extraction, DNA amplification, and phylogenetic analysis. The results obtained that PFM19 has similarities with Talaromyces islandicus CBS 388.48 by 100% based on ITS markers.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"39 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular Characterization Of Phylloplane Mold From Avicennia marina Leaves\",\"authors\":\"A. T. Lunggani, Wahyu Aji Mahardhika, Aditya Budi Utomo, E. Kusdiyantini\",\"doi\":\"10.14710/bioma.24.1.61-65\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Mangroves are a habitat for organisms and microorganisms, including phylloplane molds. Phylloplane molds are known to have various potentials such as antimicrobial, enzyme, and pigment-producing. PFM19 is an orange pigment-producing phylloplane mold. Identification of the mold is needed to determine the species of the fungus so that it can be used for further research. This study aims to identify molecularly the PFM19 mold that produces orange pigment using ITS markers. The methods used in this study included the rejuvenation of isolates, DNA extraction, DNA amplification, and phylogenetic analysis. The results obtained that PFM19 has similarities with Talaromyces islandicus CBS 388.48 by 100% based on ITS markers.\",\"PeriodicalId\":411683,\"journal\":{\"name\":\"Bioma : Berkala Ilmiah Biologi\",\"volume\":\"39 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-06-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioma : Berkala Ilmiah Biologi\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.14710/bioma.24.1.61-65\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioma : Berkala Ilmiah Biologi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14710/bioma.24.1.61-65","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
红树林是生物和微生物的栖息地,包括叶面霉菌。已知叶面霉菌具有各种潜力,如抗菌,酶和色素生产。PFM19是一种产生橙色色素的叶面霉菌。需要对霉菌进行鉴定以确定真菌的种类,以便将其用于进一步的研究。本研究旨在利用ITS标记对产生橙色色素的PFM19霉菌进行分子鉴定。本研究采用的方法包括分离株复壮、DNA提取、DNA扩增和系统发育分析。结果表明,PFM19与孤岛Talaromyces islandicus CBS 388.48的ITS标记相似性为100%。
Molecular Characterization Of Phylloplane Mold From Avicennia marina Leaves
Mangroves are a habitat for organisms and microorganisms, including phylloplane molds. Phylloplane molds are known to have various potentials such as antimicrobial, enzyme, and pigment-producing. PFM19 is an orange pigment-producing phylloplane mold. Identification of the mold is needed to determine the species of the fungus so that it can be used for further research. This study aims to identify molecularly the PFM19 mold that produces orange pigment using ITS markers. The methods used in this study included the rejuvenation of isolates, DNA extraction, DNA amplification, and phylogenetic analysis. The results obtained that PFM19 has similarities with Talaromyces islandicus CBS 388.48 by 100% based on ITS markers.