Siti Zidna Ilma Nafia, Sri Pujiyanto, Anton Budiharjo
{"title":"Isolasi, Skrining, dan Identifikasi Molekuler Bakteri Termotoleran Proteolitik dari Sumber Air Panas Nglimut Gonoharjo Kendal","authors":"Siti Zidna Ilma Nafia, Sri Pujiyanto, Anton Budiharjo","doi":"10.14710/bioma.24.1.30-35","DOIUrl":"https://doi.org/10.14710/bioma.24.1.30-35","url":null,"abstract":"The potential for the use of enzymes in industry is starting to be widely used. Protease is one of the most widely used enzymes in industry. Protease is an enzyme capable of hydrolyzing peptide bonds in proteins. Proteolytic thermotolerant bacteria are one of the important sources of thermostable enzymes that can be isolated from hot springs. The purpose of this study was to isolate and screen proteolytic thermotolerant bacteria and to identify molecularly the best isolates of proteolytic thermotolerant bacteria from the hot springs of Nglimut Gonoharjo. Isolation was carried out by graded dilution on Sodium Agar (NA) and Thermus Agar (TA) media using the spread plate method, and the proteolytic activity test using the paper disc method. Characterization of proteolytic bacterial isolates was carried out by macroscopic and microscopic morphology with gram staining. Molecular identification with 16S rRNA gene through amplification, electrophoresis, sequencing, BLAST analysis and phylogenetic reconstruction. The isolation results obtained 32 proteolytic thermotolerant isolates with irregular and round colony shape characteristics, Gram positive and Gram negative, and stem cells. The results of the enzyme test showed that isolate T5 was the best isolate with a proteolytic index value of 1.90 and was identified as Bacillus subtilis. Gram Positive and Gram Negative, and have stem cells. The results of the enzyme test showed that isolate T5 was the best isolate with a proteolytic index value of 1.90 and was identified as Bacillus subtilis.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"141 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128616860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Keanekaragaman dan Hubungan Kekerabatan Fenetik Spesies Anggota Famili Asteraceae di Jalur Pendakian Gunung Lawu Berdasarkan Karakter Morfologis","authors":"Q. Qatrunnada, Ratna Susandarini","doi":"10.14710/bioma.24.1.43-53","DOIUrl":"https://doi.org/10.14710/bioma.24.1.43-53","url":null,"abstract":"Asteraceae is one of the most diverse families in Angiosperms with an estimated of 23,600 species worldwide. One of the interesting place to explore the diversity of Asteraceae is Mount Lawu which has high potential for ecotourism. The newest track commonly used to climb to Mount Lawu is through Candi Cetho hiking track that was established in 2015. The study on the diversity of Asteraceae species on Candi Cetho hiking track has not been done before. The objective of this study was to explore the species diversity of Asteraceae and to determine their taxonomic relationship based on morphological characters. Plant samples were collected using explorative method along the hiking track, covering the altitude of 1,440 to 2,550 m above sea level. Identification of plant specimens based on morphological characters showed the existence of 13 species. Cluster analysis on 50 morphological characters was done using Euclidean distance and UPGMA method resulted in the recognition of two clusters. Based on the principal component analysis, the morphological characters which showed highest role in the grouping of Asteceae species into two clusters were the base of the upper leaf, flower color, and upper leaf attachment type.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"172 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122319984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isti Karim Murhandini, Jafron Wasiq Hidayat, Fuad Muhammad
{"title":"Struktur Komunitas Ikan di Pantai Karang Jahe Rembang","authors":"Isti Karim Murhandini, Jafron Wasiq Hidayat, Fuad Muhammad","doi":"10.14710/bioma.24.1.80-89","DOIUrl":"https://doi.org/10.14710/bioma.24.1.80-89","url":null,"abstract":"Fish play an important role both ecologically and economically. Fish also play a role in the food chain cycle, can be used as food and tourism commodities. This study aims to examine the structure of the fish community, to analyze the relationship between the number of individual fish and the physical and chemical parameters of the waters and to determine the ecotourism potential of fish diversity in Karang Jahe Beach, Rembang. The research was conducted at 5 different stations, namely tourist area, pond area, river flow, around mangroves, and marine area close to coral reef communities. Fish were caught using fishing nets and fishing trawler. Sampling at each station was repeated 3 times. The results obtained 22 species of fish belonging to 16 families, namely Epinephelus fuscoguttatus, Lutjanus bitaeniatus, Lutjanus ehrenbergii, Chanos chanos, Liza alata, Mugil sp, Oreochromis mossambicus, Pseudapocryptes elongatus, Periophthalmus sp., Eubleekeria jonesi, Eubleekeria elongate, Eubleekeria elongate Sardinella lemuru, Pennahia argentata, Trichiurus lepturus, Scomberomorus commerson, Stolephorus sp, Stolephorus indicus, Stolephorus commersoni, Chromis analyst, Terapon theraps and Lagocephalus inermis. The species diversity index is classified as low and moderate. The species evenness index is classified as low, medium and high. The dominance index is classified as low and high. Physicochemical parameters of Karang Jahe Beach waters such as temperature, pH, salinity, DO, turbidity and TDS are still classified as suitable for life, while the brightness parameter does not support fish life.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122196124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Wahyu Aji Mahardhika, Astriana Bhakti Utami, Arina Tri Lunggani, Ivan Permana Putra
{"title":"Eksplorasi Jamur Di Desa Kedung Pacul, Klaten dan Potensi Pemanfaatannya","authors":"Wahyu Aji Mahardhika, Astriana Bhakti Utami, Arina Tri Lunggani, Ivan Permana Putra","doi":"10.14710/bioma.24.1.8-23","DOIUrl":"https://doi.org/10.14710/bioma.24.1.8-23","url":null,"abstract":"Kedung Pacul, Gondangsari Village is one of the villages in Klaten Regency whose regional vegetation consists of trees, and shrubs. The shaded vegetation conditions are very suitable for overgrown mushroom. However, there is currently no record of macrofungal exploration in the village and its potential utilization. This study aims to explore mushroom in Kedung Pacul, Gondangsari Village and present the characteristics of macrofungi. The study used purposive sampling method covering roadsides, gardens, and around the house yard. Mushroom then performed macroscopic characteristics with some literature. The results of the research have found as many as 27 species including Schizophyllum commune, Pycnoporus cinnabarinus, Lentinus sp1., Neofavolus sp., Trametes sp., Tyromyces sp., Entoloma sp., Gymnopilus sp., Cyathus sp., Lentinus sajor-caju, Daldinia sp., Parasola sp., Lentinus arcularis, Leucoagaricus sp., Phallus indusiatus, Coprinus sp., Coprinellus sp., Lentinus sp2, Xylaria sp., Tremellodendron sp., Chlorophyllum sp., Leucocoprinus sp1, Leucocoprinus sp2, Termitomyces sp., Agaricus trisulphuratus, Chlorophyllum molybdites, and Agaricus sp. Several species are known to have potential as food, medicine, and cosmetics. This article is the first report on the exploration of mushroom diversity in Kedung Pacul, Gondangsari Village, Klaten Regency. Mushroom exploration efforts are still needed in the Gondangsari Village area and Klaten Regency.. ","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"104 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126226247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Kandungan Mikroplastik pada Ikan Belanak (Mugil cephalus) dan Kerang Hijau (Perna viridis) di Pantai Mangunharjo Semarang dan Pantai Sayung Demak","authors":"Ardhia Prameswari, Fuad Muhammad, Jafron Wasiq Hidayat","doi":"10.14710/bioma.24.1.36-42","DOIUrl":"https://doi.org/10.14710/bioma.24.1.36-42","url":null,"abstract":"Microplastics have polluted marine waters from the surface of the water to the sediments. High levels of microplastic contamination can cause some marine biota to become tolerant so it can disrupt the metabolism of marine biota. The aim of the study was to find out the content of the amount and type of microplastics in grey mullet, green mussel, water, and sediment also the correlation between the abundance of microplastics and the body length of fish and shellfish. The samples were obtained from four stations located in two locations. Microplastic analyzed by isolating microplastics in each sample. Fish and shellfish samples were isolated by dissolving the sample in a 10% KOH solution, then incubated at 60 ⁰C for 24 hours. Water and sediment samples were isolated by soaking in saturated NaCl solution. Data analyzed by testing the correlation using SPSS software. The average microplastics value in each type in grey mullet (particles/individu) was pellet 27,25; film 25,36; fiber 49,28; and fragment 61,08 while the average microplastics value in shellfish was pellet 8,94; film 20,61; fiber 34,00; and fragment 35,28. The average microplastics abundance in each type in water was pellet 0,72; film 1,34; fiber 2,01; and fragment 3,11 particles/mL while in sediment was pellet 1,65; film 5,25; fiber 10,09; and fragment 12,87 particles/gram. The correlation relationship between the abundance of microplastics and the length of fish and shellfish was negative.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130823248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dampak Perubahan Iklim Terhadap Fenologi Phaseolus vulgaris L Faklutas Biologi Universitas Jenderal Soedirman","authors":"Khusnul Khotimah, Eming Sudiana, Hery Pratiknya","doi":"10.14710/bioma.24.1.1-7","DOIUrl":"https://doi.org/10.14710/bioma.24.1.1-7","url":null,"abstract":"Climate change is a condition characterized by changing world climate patterns which results in erratic weather phenomena. Altitude is one of the climate control factors that have a strong influence on air temperature. There is a correlation between changes in air temperature and altitude in Indonesia. The increase in altitude causes the air temperature to decrease and the O2 content is getting thinner. Altitude also affects the biophysics and reproduction of agricultural plants a lot, which includes the response of a decrease in temperature to the growth of agricultural plants and their production. This study aims to determine the effect of climate change on the flowering phenology of green beans (Phaseolus vulgaris L.) and the diversity of insect pollinators. Sampling was done by purposive sampling to collect flower data while Scan sampling was used to collect insect data. Samples were taken as many as 24 bean plants from the population at each location. There are six locations, namely 50 m asl, 200 m asl, 400 m asl, 600 m asl, 800 m asl, and 1,000 m asl. Plant parameters observed were plant height, number of leaves, number of branches, number of flowers, size of flowers and time of bloom and diversity of insect pollinators. The data obtained were analyzed using analysis of variance (ANOVA) and regression correlation analysis. The results of the analysis showed the influence of altitude on the time the flowers first appeared, the number of flowers and flower size. Beans grow and develop optimally at an altitude of 800 - 1,000 m asl.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133561976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Tingkat Serangan Grayak Spodoptera litura Pada Cabai Rawit (Capsicum frutescens) Dengan Pemberian Bakteri Lysinibacillus sphaericus","authors":"Riza Afifah Cahyamurti, H. Purwanto","doi":"10.14710/bioma.23.2.149-158","DOIUrl":"https://doi.org/10.14710/bioma.23.2.149-158","url":null,"abstract":"Indonesia is the fourth largest chili producer in the world. In 2017, chili (Capsicum frutescens) was ranked fifth as the largest seasonal vegetable commodity products. The productivity of chili cultivation in Indonesia is hampered by pests and diseases. One of the most common pests that attack chili plants is the armyworm Spodoptera litura (Lepidoptera: Noctuidae). Lysinibacillus sphaericus bacteria have the potential as Plant Growth Promoting Rhizobacteria (PGPR) as well as a bio control agent. This study aims to obtain L. sphaericus isolates that can increase the growth of chili and to determine the effect of L. sphaericus isolates which have the potential for PGPR on the growth of the armyworm feeding on the chili plants. The research method used is experimental research conducted at the Laboratory of Entomology and Sawitsari Research Station, Universitas Gadjah Mada. This study used three replicate by inoculating one millilitre per plant. The results obtained from testing the 105 isolates in the collection of the Faculty of Biology, Universitas Gadjah Mada showed that the isolates that can increase the growth of chili plant indicated with its plant height, were isolates A42, A49, A19, A5, and A38. While the results of plant with the highest number of leaves showed the isolates A49, A42, A19, A38, and A28 were the best among the isolates tested. Some L. sphaericus isolates could increase plant growth compared to the control, but not statistically significant. These bacteria by inoculating one mililitre to plant could not be used as a bio control agent in S. litura because they did not have a significant different in leaf damage to the control.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115285511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ni Putu Nadia Pebiana, Y. Puspasari, Resty Mutiara Dewi, I. Arnyana
{"title":"Kajian Etnobotani Loloh dan Teh Herbal Lokal sebagai Penunjang Ekonomi Kreatif Masyarakat Desa Tradisional Penglipuran Kabupaten Bangli-Bali","authors":"Ni Putu Nadia Pebiana, Y. Puspasari, Resty Mutiara Dewi, I. Arnyana","doi":"10.14710/bioma.23.2.91-99","DOIUrl":"https://doi.org/10.14710/bioma.23.2.91-99","url":null,"abstract":"Penglipuran Village is a village in Bali that implements ethnobotany which is used as a medicinal plant which has been inherited in the form of a variety of traditional drinks that make use of typical plants in the village and has been widely marketed. The traditional drinks they produce are loloh cem-cem, loloh telang, turmeric loloh, plum fruit loloh and kelor and bawang berlian tea. The purpose of this article is to study the properties needed in plants used as loloh and herbal teas as well as the benefits of ethnobotany loloh and herbal teas as economic support as seen from the level of income of the people involved in their production. The writing of this article was done by taking a sample of snowballs in Penglipuran Village. Data collection techniques used in this study are: observation techniques, interview techniques, literature study techniques and data analysis using descriptive qualitative techniques. Data obtained in the field in accordance with the debates reviewed are described and interpreted qualitatively. The results obtained from this study are some plants that have been produced by economic products that are designed for local drinks, namely loloh and herbal teas. Using plants as traditional medicine by the people of Penglipuran Village, has now developed into an effort that provides economic benefits for the community. Based on the results of the study found three types of local traditional drinks (loloh) that support the welfare and economy of the Penglipuran Traditional Village community namely loloh cemcem, loloh telang flowers, and kelba tea (kelor bawang berlian) seen from the results of production in each transaction and marketing has been quite extensive.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128195787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Tingkat Keberhasilan Penanaman Mangrove Pada Lahan Bekas Tambang Timah Di Desa Rebo Kabupaten Bangka Sebagai Bentuk Pemanfaatan Lahan Dalam Wilayah Hutan Mangrove Di Pesisir Timur Pulau Bangka","authors":"A. Farhaby, M. Anwar","doi":"10.14710/bioma.23.2.143-148","DOIUrl":"https://doi.org/10.14710/bioma.23.2.143-148","url":null,"abstract":"The existence of unconventional mines (TI) which is increasingly prevalent nowadays has penetrated the coast, resulting in the mangrove ecosystem unable to develop properly. One of the areas affected by unconvencional minning activities is on the coast of Takari Beach, Bangka Regency. Takari Beach is one of the areas located on the coast of Rebo Village, Sungailiat District, Bangka Regency. One of the rehabilitation efforts carried out is by planting mangrove seedlings on Takari Beach in 2018 with a total of 500 mangrove seedlings. Mangrove rehabilitation activities are one form of land use conducted in Takari Beach. This activity also needs to be supported by the maintenance and monitoring of planting results to determine the success rate of mangrove planting activities on ex-tin mining land located in the coastal area of Takari Beach, Bangka. The results showed that the percentage of mangroves living at the observation station, namely on plot 1, was 71%. Plot 2's survival rate is 80%. The survival rate for plot 3 is 81%, the survival rate for plot 4 is 54%, and the survival rate for plot 5 is 27%. Plots 1,2 and 3 have a high survival rate, which is around 70-81%. Plots 4, and 5 have a low survival rate, which is around 27-54%. This can indicate that the success rate of mangrove survival can be said to be less successful. This could be due to the fact that there are still floating unconventional mining (TI) activities, which are operating again so that it is suspected that the tailings yield inhibits the growth rate and causes the death of mangrove seedlings.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130858795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Komposisi dan Struktur Vegetasi Riparian Strata Pohon di Kawasan Wana Wisata Curug Semirang, Ungaran, Semarang, Jawa Tengah","authors":"Raditya Pratama, J. Jumari, S. Utami","doi":"10.14710/bioma.23.2.112-118","DOIUrl":"https://doi.org/10.14710/bioma.23.2.112-118","url":null,"abstract":"Wana Wisata Curug Semirang is a tourist area in Semarang Regency based on ecotourism. As an ecotourism area, Curug Semirang contribute as conservation area in order to protection and preservation of nature. Along with tourism development, biodiversity in Curug Semirang is increasingly threatened. The purpose of this study is to examine the composition and structure of riparian in Wana Wisata Curug Semirang Area. The method used in vegetation is a quadratic plot with a line transect. Vegetation observation was carried out at 3 stations which were divided into 15 plots covering tree strata and poles. Vegetation analysis was obtained with the value of density, dominance, frequency, and important value index. The results obtained 75 plant species covering 42 families, in the tree strata there are 16 species in 12 families, and the pole strata there are 20 species in 14 families. The highest INP of tree strata at station 1 was pine (Pinus merkusii) 180.04%, stations 2 and 3 were kemadu (Laportea sinuata) 147.53% and 102.76%, respectively. The highest IVI of the pole strata at stations 1 and 2 is coffee (Coffea arabica) 123.98% and 140.5%, and station 3 is rotan (Calamus rotang) 113%.","PeriodicalId":411683,"journal":{"name":"Bioma : Berkala Ilmiah Biologi","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115144924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}