Saudi Journal of Pathology and Microbiology最新文献

{"title":"Follicular Carcinoma Thyroid with Focus of Squamous Cell Carcinoma - A Case Report","authors":"Alina Mariam Varghese, S. S","doi":"10.36348/sjpm.2023.v08i03.001","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i03.001","url":null,"abstract":"Collision tumors of the thyroid gland are a rare entity. Primary squamous cell carcinoma of the thyroid (PSCCT) is a malignant epithelial tumor, composed entirely of cells with squamous differentiation and accounting for less than 1% of all malignancies of the thyroid gland. PSCCT arises from other thyroid diseases such as Hashimoto’s thyroiditis, tall cell variant papillary carcinoma, follicular carcinoma, and anaplastic carcinoma1. We describe a patient with the combination of a well-differentiated squamous cell carcinoma and follicular carcinoma of the thyroid.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122018573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of MIC of Different Antibiotics against Intermediate Isolates of S. aureus at a Tertiary Care Hospital by E-test","authors":"Mst. Romena Khatun, Khandaker Md Faisal Alam, Md. Khaled Shahrear, Rozina Akter Zahan, J. Sazzad","doi":"10.36348/sjpm.2023.v08i02.003","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i02.003","url":null,"abstract":"Background: The E-test involves using a predefined antibiotic gradient on a strip, which is then placed on an agar plate containing the bacterial culture. The point at which the antibiotic concentration on the strip causes inhibition of bacterial growth is taken as the MIC. This method allows for the rapid and accurate determination of the MIC of antibiotics against specific bacterial strains and can aid in selecting appropriate antibiotics for treatment. Objectives: The aim of this study was also to include a comparison of the results with those of previous studies and an evaluation of the study's limitations. Method: A cross-sectional microbiological study was conducted at the Department of Microbiology, and the Department of ENT Rajshahi Medical College, Bangladesh, from January to December 2019. This section will provide a detailed description of the methods used in the study, including the study design, sampling methods, and procedures for collecting and analyzing the data. The methods section will also explain the E-test method used to determine MIC, including the procedure for performing the test and the criteria for interpreting the results. Results: A total of 96 samples, among 73 isolates from 68 culture-positive cases, 37 isolates showed intermediate susceptibility towards selected antibiotics such as beta-lactams, aminoglycosides and quinolones by disc diffusion method. Regarding MIC breakpoint in terms of susceptibility, out of 21 intermediate isolates of S. aureus, 16(76.2%) were susceptible, 01(4.8%) was intermediate, and 04(19%) were resistant to different antibiotics by E-test. Conclusion: In general, the conclusion of such an article would likely summarize the study's findings, such as the MIC values for the different antibiotics tested and how they compare to established MIC breakpoints, and any significant observations or trends noted. It may also discuss the clinical relevance of the results and provide recommendations for future research.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133259559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth Kinetics of Bacteria Isolated from Laboratory Prepared Cheese Made From Different Milk Sources","authors":"P. Oleghe, C. Oshoma, Kin Agholor","doi":"10.36348/sjpm.2023.v08i02.002","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i02.002","url":null,"abstract":"Background: Dairy food products constitute a major part of our routine diets and it is fundamental to the food industry globally. However, the need for quality control cannot be over-emphasized. A microbiological guideline must be used to define the differences between unacceptable and acceptable food products as food-borne related diseases have been associated with milk and dairy products. Aim of Study: The study aims to evaluate the growth kinetics of cheese prepared in the laboratory from different milk types over a 96 hour period in order to determine its bacteriological quality and assess the effect of storage time on the pH of the various cheeses. Methods: The laboratory cheese was prepared from fresh Goat, cow, sheep and soybeans using the traditional method. Thereafter, 10g of the cheese samples were stored in sterile 250 ml conical flasks at temperature of 28±2 oC for duration of 96 h. The samples were analyzed every 12-24 h interval using Nutrient Agar, MacConkey Agar, Mannitol Salt Agar and De Mann Rogosa and Sharpe Agar for their bacterial, coliform, staphylococcal and lactobacilli load respectively. Results: At 96 h the highest heterotrophic bacterial load, coliform, staphylococcal and lactic acid bacteria counts were observed in sheep milk cheese (8.27 ± 0.02 Log10cfu/g), (7.10 ± 0.33 Log10cfu/g), (7.40 ± 0.34 Log10cfu/g) and cow milk cheese (8.19 ± 0.05 Log10cfu/g) respectively. At 0 h the highest pH was recorded in Soybean milk cheese (5.76±0.01) while the least was cow milk cheese (4.97±0.11). The highest pH at 96 h was Soybean milk cheese (6.96±0.01) while the least pH was recorded in sheep milk cheese (6.12±0.00). Comparing results of this present study with that of the European Union microbiological regulations (EUMR) and the Gulf Standards Organization (GSO) for foods, the bacteria load of all cheese samples at 12 h was within the acceptable or permissible limit. Conclusion: The present study indicates that laboratory prepared cheese contains a repertoire of significant public health microorganisms in numbers above the permissible limit. It is recommended that appropriate aseptic procedures should be strictly upheld by dairy handlers across the entire chess production value chain.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"185 5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124924976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutation Analysis and Clinicopathological Implications of Isocitrate Dehydrogenase 1/2 Mutation in Acute Myeloid Leukemia Patients in North India: A Tertiary Centre Based Study","authors":"Akanksha Agarwal, Mili Jain, R. Kushwaha, S. Tewari, N. Verma, A. Tripathi, M. Goel, Ashutosh Kumar","doi":"10.36348/sjpm.2023.v08i02.001","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i02.001","url":null,"abstract":"Acute myeloid leukemia has been classified on the basis of morphology, cytochemistry and genetic profile. IDH gene is an epigenetic modifier whose mutation is involved in pathogenesis of various malignancies. This study was planned to study the prevalence of IDH1/2 mutation in denovo AML cases at a tertiary care Centre in North India and also to study its clinicopathological effect in these cases. We evaluated 60 patients registered at our Centre for diagnosis and treatment of AML. Routine investigations, bone marrow examination, flow cytometry were done followed by PCR and sequencing. Out of the total 60 patients of AML 4(6.7%) patients had IDH1R312 mutation and 5(8.3%) Patients had IDH2R172 mutation. IDH2 R140 mutation was not detected in any sample. IDH mutation was significantly associated with high risk AML group. No significant clinicopathological correlation was seen. In this study significant association was observed between IDH mutation and high risk AML cases. There was no paediatric case with IDH mutation. IDH mutation and AML might have an age related association.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133593913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post Radiation Transformation to a Sarcomatous Meningioma: a Case Report in an Adolescent Male with NF2 Genetic Mutation","authors":"Layla Albayyat, Mahmmoud Taha, M. Abdulkader","doi":"10.36348/sjpm.2023.v08i01.004","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i01.004","url":null,"abstract":"Atypical meningioma is often treated adequately by complete surgical resection and subsequent radiation therapy. Nevertheless, radiation therapy should be reassessed in the management of younger patients and particularly in individuals harboring NF2 genetic mutation. To the best of our knowledge this is the third case of post radiation transformation of atypical meningioma in an adolescent male after reviewing the available English literature. Kew wards: Post radiation, atypical meningioma, transformation, sarcomatous meningioma, NF2 mutation.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130473341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solid Variant of Adenoid Cystic Carcinoma Coexisting with Invasive Squamous Cell Carcinoma in Uterine Cervix – A Case Report with Immunoprofile","authors":"P. Roshni, B. Pavithra","doi":"10.36348/sjpm.2023.v08i01.003","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i01.003","url":null,"abstract":"Primary adenoid cystic carcinoma of cervix is a rare entity. Co-existence of adenoid cystic carcinoma with squamous cell carcinoma is even more rare. Here we present the case of an 85 year old postmenopausal lady who presented with on and off vaginal bleeding and was found to have a hard cervical mass. Hysterectomy with bilateral salpingo-oophorectomy was done and histopathological evaluation revealed coexisting solid variant of adenoid cystic carcinoma and squamous cell carcinoma. High grade squamous intraepithelial lesion was seen adjacent to invasive squamous cell carcinoma. Adenoid cystic carcinoma was positive for CD117 and squamous cell carcinoma was positive for p63. Both the components were also immunoreactive for p16.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116137443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uterine Leiomyosarcoma: A Rare Entity","authors":"Dr. Mohammed Faisal Mahfooz, D. S","doi":"10.36348/sjpm.2023.v08i01.002","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i01.002","url":null,"abstract":"Uterine leiomyosarcoma is a rare uterine malignancy that arises from the smooth muscle of uterine wall. Presenting symptoms may be vague and mimic other benign uterine conditions. They are notoriously aggressive with poor prognosis. We report a case of a 35-year-old female, who presented with abdominal distension with pain and vomiting. A non-tender mass measuring 14X12 cms was felt on palpation in the same region. CT scan revealed a large, relatively well defined, hypodense pelvic mass in retro-uterine pouch. Patient underwent exploratory laparotomy with primary resection of tumour with pan-hysterectomy with pelvic lymph node resection. Histopathologically, it was diagnosed as pleomorphic sarcoma probably leiomyosarcoma of uterus which showed immunostain Smooth Muscle Antigen (SMA) positive and Epithelial Membrane Antigen (EMA) negative. It was Estrogen Receptor(ER) negative. The rarity as well as pathological diversity makes this study beneficial and this definitive reporting will be helpful in diagnosis of smooth muscle tumours with challenging histologic features.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127102208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Intraoperative Imprint Cytology and Frozen Section in Determination of Tumor and Tumor Margins","authors":"Dr. Nishana Chackam Pallialil, Dr. Vamseedhar Annam, D. S, Dr. Maheswari, P","doi":"10.36348/sjpm.2023.v08i01.001","DOIUrl":"https://doi.org/10.36348/sjpm.2023.v08i01.001","url":null,"abstract":"Intraoperative pathology consultation is often required for guiding immediate surgical decisions so as to limit the extent of surgery. Accurate intraoperative diagnosis is crucial. The present study evaluates the accuracy of imprint cytology and frozen section in intraoperative consultation for tumor and tumor margins. A total of 157 tissue specimens for type of lesion, tumor margins and lymphnode metastasis were included. Imprint was taken by holding the tissue bit with forceps and touching it onto a clean glass slide without undue pressure or lateral movements. They were stained with Papanicolaou, May-Grunwald Giemsa and Hematoxylin and Eosin [H&E] stains. The tissue bits taken were then embedded and frozen. Section cutting was done using cryostat and were stained using rapid Haematoxylin and Eosin stain, and examined under a light microscope. Later, the tissue was fixed in 10% neutral buffered formalin for routine histopathological processing then stained with H&E stain. Based on the histological diagnosis, the accuracy of intraoperative imprint cytology and frozen section were evaluated. A total of 157 tissue specimens were received during the study period, which were classified into specimens for type of tumor [145 specimens], tumor margins [54 specimens] and lymph node metastasis [7 specimens] for analysis. The overall diagnostic accuracy of imprint cytology in detection of type of tumor was 97.9% and that of frozen section was 98.6%. It can be concluded that imprint cytology typically provides the accurate diagnosis without the need for a sophisticated machine such as cryostat which is required for frozen section analysis.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"15 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120907968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinicopathologic Evaluation of Wilms’ Tumor in a Tertiary Care Children Hospital","authors":"P. M, A. A, M. B, Sadiya S, Monowara M, P. K, R. A","doi":"10.36348/sjpm.2022.v07i12.005","DOIUrl":"https://doi.org/10.36348/sjpm.2022.v07i12.005","url":null,"abstract":"Background and Objective: Wilms’ tumor has been recognized as the most common primary malignant tumor of kidney at childhood. It comprises 5-6% of tumors in the childhood period, and manifests with various clinical symptoms. Since there have been no sufficient studies in this field in Bangladesh, therefore, this study was conducted to investigate its histopathology and different clinical symptoms. Materials and Methods: This study was carried out on existing data from 70 children with a diagnosis of Wilms’ tumor at Bangladesh Shishu Hospital during the years 2015-2020. In this regard, personal and disease-related characteristics of patients including age, sex, tumor stage, histopathology, and involved kidney were evaluated and SPSS software and other statistical tests were used for data analysis. Results: The most common age of disease incidence was 2-4 years. In this regard, male and female ratio was 1.5. Meanwhile, the prevalence of an abdominal mass as the most common symptom was 85.0%. Left kidney was involved in 50% of cases and 58 (82.86%) of patients had a favorable histology. In addition, there was a significant correlation between site of kidney involvement and tumor histology (p<0.005). Conclusion: Considering the achieved advances in the diagnosis and treatment of Wilms’ tumor, early diagnosis with regard to clinical symptoms can have a valuable role in its effective management.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"109 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133305820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uterine Smooth Muscle Tumor of Uncertain Malignant Potential – An Unusual Case Report","authors":"M. Deepti, L. Krishna, S. Srikanth, C. Swathi","doi":"10.36348/sjpm.2022.v07i12.004","DOIUrl":"https://doi.org/10.36348/sjpm.2022.v07i12.004","url":null,"abstract":"The term smooth uterine muscle of uncertain malignant potential (STUMP) indicates a group of uterine smooth muscle tumors (SMTs) that cannot be diagnosed unequivocally as benign or malignant. Diagnosis, surgical management, and follow-up of this neoplasm remain controversial, especially in pre-menopausal women with fertility desire, due to the non aggressive behaviour and prolonged survival rate when compared to leiomyosarcomas. Studies of STUMP are limited in population and rarity results in few analysis of its fertility outcomes and oncologic prognosis. STUMP has a lower tumor growth rate and recurrence is often delayed by years after initial event compared to high grade leiomyosarcomas which have an aggressive clinical course and behavior thus making it very important to differentiate stump from leiomyosarcomas .Recurrencerate ranges from 8.7% to 11%.","PeriodicalId":407259,"journal":{"name":"Saudi Journal of Pathology and Microbiology","volume":"102 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116061650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}