Glasnik Hemicara i Tehnologa Bosne i Hercegovine最新文献

{"title":"Peroxidase activity of hemoglobin and heme destruction in the presence of hydrogen peroxide and CT-DNA","authors":"","doi":"10.35666/ghtbh.2020.55.04","DOIUrl":"https://doi.org/10.35666/ghtbh.2020.55.04","url":null,"abstract":"The aim of this study was to investigate the peroxidase activity of Hb with different concentrations of hydrogen peroxide and compare it with hypochlorous acid effect on Hb. Hypochlorous acid at higher concentrations decomposed Hb and heme, releasing fee iron ion from the metal center. High concentrations of hydrogen peroxide switched the peroxidase activity of Hb towards the partial Hb and heme destruction. Heme alone was degraded showing that the Hb conformation and protein environment protects Hb from the distraction in the presence of highly increased hydrogen peroxide concentration that occurs as a result of oxidative stress. In the presence of CT-DNA acted inhibition of the peroxidase activity of Hb was observed signaling inhibited hydrogen peroxide consumption.","PeriodicalId":40710,"journal":{"name":"Glasnik Hemicara i Tehnologa Bosne i Hercegovine","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2020-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80296798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qualitative and quantitative determination of ligustilide as bioactive marker in apiaceous botanicals","authors":"","doi":"10.35666/ghtbh.2020.55.02","DOIUrl":"https://doi.org/10.35666/ghtbh.2020.55.02","url":null,"abstract":"Variety of bioactivities has been associated with ligustilide present in root of Angelica sinensis, and predominantly used for the treatment of irregular menstrual cycles and premenstrual syndrome. Recent pharmacological studies showed that medicinal plants containing ligustilide, have anti-inflammatory effects and contribute to the improvement of cognitive functions, alleviate brain damage, inhibit tumor necrosis factor of some cell lines, and have nephron-protective effects and neuroprotective activity. In this work, quantification of ligustilide using quantitative 1H NMR (qHNMR) in sealed tubes was performed. Four plant species were investigated: A. sinensis, Ligusticum porteri, Ligusticum striatum, andLigusticum sinense. Modified supercritical CO2 extraction of essential oil from root of four investigated species, was performed. qHNMR spectroscopy showed following percentage of ligustilide: L. porteri essential oil 3.74 (%); L. sinense essential oil 1.16 (%); L. striatum essential oil 6.61 (%) and A. sinensis essential oil 14.56 (%). The highest percentage of oil was obtained from the root of L. porteri but the highest percentage of ligustilide was obtained from A. sinensis essential oil.","PeriodicalId":40710,"journal":{"name":"Glasnik Hemicara i Tehnologa Bosne i Hercegovine","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2020-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82339326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and characterization of Fe(III) complex with thiosemicarbazide-based ligand","authors":"","doi":"10.35666/ghtbh.2020.55.03","DOIUrl":"https://doi.org/10.35666/ghtbh.2020.55.03","url":null,"abstract":"Complex iron(III) salicylaldehyde thiosemicarbazone with a formula Na[Fe(STSC)2] have been synthesized. Ligand, salicylaldehyde thiosemicarbazone (SCT) was prepared through the condensation reaction of salycilaldehide with thiosemicarbazide. Na[Fe(STSC)2] has been synthesized by reacting FeCl3 with thiosemicarbazide based ligand bearing ONS donor atoms. Complex has been formulated and characterized by mass spectrometry, infrared and UV/visible spectroscopy. The facts showed the formation of a complex in metal:ligand stoichiometric ratio 1:2. The ligand is coordinated as an ONS tridentate dianion via oxygen atom after the deprotonation of the phenolic OH-group, azomethine nitrogen and sulphur in thiol form of the deprotonated thiosemicarbazide residue. Antioxidant activity was determined, where ligand showed significant activity, while complex, at low concentration, exhibited almost no activity.","PeriodicalId":40710,"journal":{"name":"Glasnik Hemicara i Tehnologa Bosne i Hercegovine","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2020-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83370720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenolic Composition, Antioxidant and Antimicrobial Activity of Cotoneaster Medik. Species from Bosnia and Herzegovina","authors":"","doi":"10.35666/ghtbbh.2020.54.01","DOIUrl":"https://doi.org/10.35666/ghtbbh.2020.54.01","url":null,"abstract":"Although the genus Cotoneaster Medik. includes mainly ornamental species, there are\u0000some data regarding its biological activity. The purpose of this study was to analyze the content of phenolic compounds, acetylcholinesterase inhibition, antioxidant and antimicrobial activity of methanolic extracts of leaf and bark of C. integerrimus Medik., C. tomentosus (Aiton) Lindl. and C. horizontalis Decne. The C. tomentosus leaf extract exhibited the highest content of total phenols (135.86 mg GAE/g) and flavonoids (18.17 mgQE/g), and also the most potent antioxidant activity against nonbiogenic free radicals, while the highest inhibition of acetylcholinesterase had the leaf extract of C. horizontalis (IC50 0.34 mg/mL). All extracts showed a significant level of antibacterial and antifungal activity against tested microbial strains. The largest inhibition zones were observed against Candida albicans treated with C. integerrimus leaf extract (30.50±0.50 mm). Furthermore, C. integerrimus extract was the most effective in the majority of bacterial strains tested. The results indicated that methanolic extracts of the investigated Cotoneaster species have promising bioactive and therapeutic potentials","PeriodicalId":40710,"journal":{"name":"Glasnik Hemicara i Tehnologa Bosne i Hercegovine","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90449328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum Iron Concentration and Lipid Profile in Type 2 Diabetes Patients","authors":"","doi":"10.35666/ghtbh.2020.54.02","DOIUrl":"https://doi.org/10.35666/ghtbh.2020.54.02","url":null,"abstract":"Recent studies have been showed important role of elevated iron levels in pathogenesis of Type 2 Diabetes mellitus (T2D) and insulin resistance. The aim of this study was to determine serum of free iron concentration in T2D patients and find out associations with lipid profile. The study included 51 participants (27 healthy control and 24 no treated diabetes patients), with ages from 45 to 65 45-65 ages and both gender. As expected, concentrations of serum iron were elevated in diabetic patients compare to healthy subjects while statistical significant difference were shown between iron levels in control group and group with good control of glycaemia (p<0.05). In addition, there was a significant positive correlation between free iron concentration and LDL cholesterol levels and negative significant correlation between iron concentrations with HDL cholesterol in diabetics (p<0.05). These findings suggest that increase serum of free iron concentrations may have an important role and influence in development of disease, especially in lipid metabolism and profile as well in risk of further complications of diabetes.","PeriodicalId":40710,"journal":{"name":"Glasnik Hemicara i Tehnologa Bosne i Hercegovine","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75208249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the Antioxidant Synergisms and Antagonisms among Caffeic, Ferulic and Rosmarinic Acids using the Briggs-Rauscher Reaction Method","authors":"","doi":"10.35666/ghtbh.2020.54.05","DOIUrl":"https://doi.org/10.35666/ghtbh.2020.54.05","url":null,"abstract":"Phenolic acids have been attracting huge attention over recent years due to their prominent antioxidant activity and potential health benefits. In this study, the antioxidant activity of caffeic, ferulic, and rosmarinic acids was evaluated using Briggs-Rauscher reaction method. In addition to single phenolic acids at different concentrations (50, 100, 200 and 250 µM), equimolar mixtures of two phenolic acids and all three phenolic acids were tested. The best ability to inhibit oscillations, i.e. the highest antioxidant activity showed rosmarinic acid at a concentration of 250 µM. Inhibition time of the Briggs-Rauscher oscillating reaction mixture was obtained experimentally for the different combinations of phenolic acids and compared with theoretical values calculated by adding up the effects of phenolic acids analyzed individually. The most of tested phenolic acids mixtures showed a difference in the antioxidant activity when compared to individual values of their constituents. The highest synergistic effect showed a mixture of caffeic acid and ferulic acid at a concentration of 250 µM while the highest antagonistic effect showed the mixture of caffeic acid, ferulic acid and rosmarinic acid at same concentration","PeriodicalId":40710,"journal":{"name":"Glasnik Hemicara i Tehnologa Bosne i Hercegovine","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82361222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromatographic Methods for the Determination of Primula Acid 1 Content in Primulae extractum fluidum","authors":"","doi":"10.35666/ghtbh.2020.54.03","DOIUrl":"https://doi.org/10.35666/ghtbh.2020.54.03","url":null,"abstract":"Primula veris L. (Primulaceae)is healing plant, whose root is officially used to treat cough associated with cold. Other reported indications are respiratory, thoracic and mediastinal disorders. These effects are result of high contents of triterpenoid saponins and phenolic glycosides. Primula acid 1 (PA 1, also primulasaponin 1) is main active component in Primula elatior L. This paper presents an optimized high pressure liquid chromatography (HPLC) method for the determination of primula acid 1 content in Primulae extractum fluidum. TLC was performed to check for the presence of the substance of interest. The determination was performed by reversed phase chromatography using C18 as a stationary phase. The mobile phase used for separation consisted of 0.2% H3PO4 and acetonitrile. This method was validated through different parameters. The detection limit for primula acid 1 was LD=10.41 µg/ml, and the quantification limitwas LQ=34.69 µg/ml. In order to determine the content of primula acid 1, a calibration curve was constructed, and the content of primula acid 1 was calculated by the equation of the calibration curve and was 0.2793 mg per gram of extract. The results and simple preparation of sample show that HPLC is the method of choice for this type of analysis.","PeriodicalId":40710,"journal":{"name":"Glasnik Hemicara i Tehnologa Bosne i Hercegovine","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82740608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Investigation and Antioxidative Capacity of Triterpenes Present in Plant Species Belonging to Lamiaceae Family","authors":"","doi":"10.35666/ghtbh.2020.54.04","DOIUrl":"https://doi.org/10.35666/ghtbh.2020.54.04","url":null,"abstract":"Triterpenes are persistently associated with observed bioactivities of extracts obtained from plant material that contains these very important natural products. Many species belonging to Lamiaceae family have been used for the presence of essential oil and very little is known about the presence of the triterpene substances in this family. Qualitative and quantitative analyses of this very important substances, in the aerial parts of eight species, all belonging to Lamiaceae family, were investigated in this study. Different extracts containing triterpene substances were tested by DPPH method to evaluate their antioxidative capacity. TLC and HPLC methods, used for the analytical determination of triterpenes, showed the presence of betulin, betulinic acid, ursolic acid and oleanolic acid. Betulin (3.2 mg/g) and betulinic acid (37.1 mg/g) were the most abundant triterpene components in the hexane extracts of Rosmarinus officinalis L. Ursolic acid (0.14 mg/g) was the most abundant triterpene compound in the hexane extract of Thymus pulegioides L. All tested samples demonstrated DPPH scavenging activity in a concentration dependant manner, with a wide range of IC50 values from 0.4 mg/mL to 3.3 mg/mL.","PeriodicalId":40710,"journal":{"name":"Glasnik Hemicara i Tehnologa Bosne i Hercegovine","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2020-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77293653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bosna i Hercegovina u antičko doba u kontekstu zapadnobalkanske historiografije u prve dvije decenije 21. stoljeća","authors":"Amra Šačić Beća","doi":"10.5644/pi2020.186.02","DOIUrl":"https://doi.org/10.5644/pi2020.186.02","url":null,"abstract":"Zbog ratnih dešavanja u periodu 1992–1995. došlo je do primjetne stagnacije u procesu arheoloških i historijskih istraživanja rimskog razdoblja bosanskohercegovačke historije. Međutim, u posljednje dvije decenije u regiji je objavljen značajan broj radova koji se bave problematikom peregrinskih civitates, municipalnih jedinica, te romanizacijom i urbanizacijom prostora današnje Bosne i Hercegovine. Autori poput Borisa Olujića, Alke Kunić Domić, Siniše Dujmušića-Bilića, Danijela Džine, Snežane Ferjančić, Svetlane Lome, Dragane Grbić (Nikolić), Domagoja Tončinića, Marina Zaninovića i Radmile Zotović svoju naučnu pažnju su u najvećoj mjeri usmjerili na pojedinačne indigene zajednice, vojna pitanja i municipalnu organizaciju. Kada je riječ o naučnicima koji se pišući u širem kontekstu o Dalmaciji, odnosno Panoniji, dotiču problematike administrativnog uređenja na prostoru današnje Bosne i Hercegovine, posebno treba istaći radove slovenske naučnice Marjete Šašel Kos. U kontekstu hronologije jasno se uočava da se najveći broj radova koji su objavljeni u posljednje dvije decenije bavi temama iz perioda Augustove i Tiberijeve vladavine. Akcenat je na izučavanju položaja indigenih zajednica u periodu vojnih sukoba s Rimom, dok je problematika njihovog razvoja nakon Bellum Batonianum (6–9. god.) dosta zanemarena. Također, može se primijetiti da nema revizije ranijih mišljenja u vezi s nastankom i razvojem municipalnih jedinica. To je rezultiralo time da gotovo uopće nema radova koji obrađuju administrativne teme koje se odnose na drugo i treće stoljeće nove ere. U radovima koji se odnose na period kasne antike uočava se prisustvo određenih, savremenih političkih ideologija karakterističnih za devedesete godine prošlog stoljeća. U zapadnobalkanskoj historiografiji u prve dvije decenije 21. stoljeća prvi put se rimski utjecaj na tlu savremene Bosne i Hercegovine počinje izučavati primjenom naučnih metoda anglosaksonske klasične škole. Postprocesualisti i strukturalisti s prostora bivše Jugoslavije odbacuju ranija viđenja pojmova pleme, romanizacija i Ilirik te ih vide kao produkt imperijalističke historiografije.","PeriodicalId":40710,"journal":{"name":"Glasnik Hemicara i Tehnologa Bosne i Hercegovine","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85584500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Forgotten Thousands: The Historiography of World War II Rescues of Allied Airmen in Yugoslavia","authors":"Robert J. Donia","doi":"10.5644/pi2020.186.11","DOIUrl":"https://doi.org/10.5644/pi2020.186.11","url":null,"abstract":"During World War II, Allied bombing of German-controlled petroleum refineries in Ploesti, Romania, diminished Axis fuel production but cost the Allies hundreds of planes and thousands of lives. Crews of many damaged planes flew partway back to Italy but were forced to crash-land their craft or bail out over Yugoslavia, where many landed on territory controlled by Partisans or Chetniks. Local Yugoslavs (mainly peasants), as well as both Chetniks and Partisans, welcomed them and gave them shelter. They were then evacuated by Allied transport aircraft (principally C-47s) that landed on makeshift airstrips maintained by Partisans or Chetniks. The historiography of these rescues may be divided into document-based studies, prepared principally by US military personnel based on official records; and memory-based studies by pro-Mihailović authors based principally on participant memoirs. Whereas memory-based studies uniformly adopted a Serb nationalist viewpoint, document-based studies showed no favoritism and portrayed various factions working in parallel to rescue Allied airmen. After Milošević fell in 2000, the Foreign Minister of Serbia and Montenegro, Vuk Drašković, in cooperation with the US Embassy, united the movement to valorize downed airmen and local efforts to rehabilitate Mihailović. Whether deliberately or not, US officials thereby undercut human rights activists in Serbia, and non-Serbs throughout the former Yugoslavia, who saw Mihailović as a war criminal, collaborator, and inspiration for war crimes and genocide in the wars of the 1990s.","PeriodicalId":40710,"journal":{"name":"Glasnik Hemicara i Tehnologa Bosne i Hercegovine","volume":null,"pages":null},"PeriodicalIF":0.3,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72835899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}