过氧化氢和CT-DNA存在下血红蛋白和血红素破坏的过氧化物酶活性

IF 0.2 Q4 CHEMISTRY, MULTIDISCIPLINARY

Glasnik Hemicara i Tehnologa Bosne i Hercegovine Pub Date : 2020-12-29 DOI:10.35666/ghtbh.2020.55.04

{"title":"过氧化氢和CT-DNA存在下血红蛋白和血红素破坏的过氧化物酶活性","authors":"","doi":"10.35666/ghtbh.2020.55.04","DOIUrl":null,"url":null,"abstract":"The aim of this study was to investigate the peroxidase activity of Hb with different concentrations of hydrogen peroxide and compare it with hypochlorous acid effect on Hb. Hypochlorous acid at higher concentrations decomposed Hb and heme, releasing fee iron ion from the metal center. High concentrations of hydrogen peroxide switched the peroxidase activity of Hb towards the partial Hb and heme destruction. Heme alone was degraded showing that the Hb conformation and protein environment protects Hb from the distraction in the presence of highly increased hydrogen peroxide concentration that occurs as a result of oxidative stress. In the presence of CT-DNA acted inhibition of the peroxidase activity of Hb was observed signaling inhibited hydrogen peroxide consumption.","PeriodicalId":40710,"journal":{"name":"Glasnik Hemicara i Tehnologa Bosne i Hercegovine","volume":null,"pages":null},"PeriodicalIF":0.2000,"publicationDate":"2020-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Peroxidase activity of hemoglobin and heme destruction in the presence of hydrogen peroxide and CT-DNA\",\"authors\":\"\",\"doi\":\"10.35666/ghtbh.2020.55.04\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The aim of this study was to investigate the peroxidase activity of Hb with different concentrations of hydrogen peroxide and compare it with hypochlorous acid effect on Hb. Hypochlorous acid at higher concentrations decomposed Hb and heme, releasing fee iron ion from the metal center. High concentrations of hydrogen peroxide switched the peroxidase activity of Hb towards the partial Hb and heme destruction. Heme alone was degraded showing that the Hb conformation and protein environment protects Hb from the distraction in the presence of highly increased hydrogen peroxide concentration that occurs as a result of oxidative stress. In the presence of CT-DNA acted inhibition of the peroxidase activity of Hb was observed signaling inhibited hydrogen peroxide consumption.\",\"PeriodicalId\":40710,\"journal\":{\"name\":\"Glasnik Hemicara i Tehnologa Bosne i Hercegovine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2020-12-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Glasnik Hemicara i Tehnologa Bosne i Hercegovine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.35666/ghtbh.2020.55.04\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Glasnik Hemicara i Tehnologa Bosne i Hercegovine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.35666/ghtbh.2020.55.04","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

摘要

本研究的目的是研究不同浓度过氧化氢对血红蛋白过氧化物酶活性的影响，并比较其与次氯酸对血红蛋白的影响。较高浓度的次氯酸分解血红蛋白和血红素，从金属中心释放出铁离子。高浓度的过氧化氢使血红蛋白的过氧化物酶活性转向部分血红蛋白和血红素的破坏。血红素单独被降解，表明Hb构象和蛋白质环境保护Hb免受氧化应激引起的过氧化氢浓度高度增加的干扰。在存在CT-DNA的情况下，观察到Hb过氧化物酶活性的抑制信号抑制过氧化氢的消耗。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Peroxidase activity of hemoglobin and heme destruction in the presence of hydrogen peroxide and CT-DNA

The aim of this study was to investigate the peroxidase activity of Hb with different concentrations of hydrogen peroxide and compare it with hypochlorous acid effect on Hb. Hypochlorous acid at higher concentrations decomposed Hb and heme, releasing fee iron ion from the metal center. High concentrations of hydrogen peroxide switched the peroxidase activity of Hb towards the partial Hb and heme destruction. Heme alone was degraded showing that the Hb conformation and protein environment protects Hb from the distraction in the presence of highly increased hydrogen peroxide concentration that occurs as a result of oxidative stress. In the presence of CT-DNA acted inhibition of the peroxidase activity of Hb was observed signaling inhibited hydrogen peroxide consumption.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Glasnik Hemicara i Tehnologa Bosne i Hercegovine CHEMISTRY, MULTIDISCIPLINARY-

自引率

0.00%

发文量