中南大学学报(医学版)最新文献

{"title":"Hypophysitis associated with immune check piont inhibitors treatment: A case report and literature review.","authors":"Jie Wei, Xingxing Han, Yutong Ye, Tiantian Qi, Tianyan Zhou, Feng Lu, Xinlin Yan, Bin Lu","doi":"10.11817/j.issn.1672-7347.2024.230536","DOIUrl":"10.11817/j.issn.1672-7347.2024.230536","url":null,"abstract":"<p><p>Immune checkpoint inhibitors (ICPis) significantly improves survival in a number of cancer patients by blocking immunosuppressive molecules and reactivating the function of effector T cells to specifically kil tumor cells. This article reports a case of secondary hypoadrenocorticism caused by programmed death 1 (PD-1) inhibitor related hypophysitis. A 65-year-old male patient received immunotherapy for right lung squamous cell carcinoma invading the chest wall (cT4N2M0) for 4 times. Two weeks after the last immunotherapy treatment, the patient experienced poor appetite and fatigue. Examination results indicated severe hyponatremia, and there was no improvement even after repeated supplementation with high-concentration sodium chloride. After further examination, glucocorticoid supplementation was given to the patient and his clinical symptoms were significantly improved. It is recommended that patients receiving ICPis should be asked in detail about their medical history before initiating treatment, baseline screening should be carried out reasonably, and regular follow-up about endocrine gland hormone and related biochemical indexes after treatment should be carried out. Meanwhile, it is necessary to pay attention to the related symptoms and signs of patients in order to find endocrine gland adverse reactions in time.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 7","pages":"1082-1088"},"PeriodicalIF":0.0,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11495972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142956343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Needs of parenting interventions based on mobile health technology in parents of depressed adolescents: A qualitative study.","authors":"Yiwen Hu, Dan Tian, Chun Liu, Simin Xie, Min Yang","doi":"10.11817/j.issn.1672-7347.2024.240131","DOIUrl":"10.11817/j.issn.1672-7347.2024.240131","url":null,"abstract":"<p><strong>Objectives: </strong>Parenting interventions aim to enhance positive parenting behaviors through skills training and guidance for parents. International studies have shown that mobile health-based parenting effectively alleviate depressive symptoms in adolescents. However, such practices are still in their infancy in China. Cultural differences between China and other countries necessitate the development of parenting interventions tailored to the Chinese context. This study aims to explore the willingness and needs of parents of adolescents with depression for mobile health-based parenting interventions, providing a reference for designing such programs to prevent or mitigate depressive symptoms in adolescents.</p><p><strong>Methods: </strong>Using purposive sampling, semi-structured interviews were conducted with 15 parents of adolescents with depression hospitalized at the Mental Health Research Institute at the Second Xiangya Hospital of Central South University between July and September 2023. Data were analyzed using Nvivo12.0 software and Colaizzi's seven-step phenomenological method.</p><p><strong>Results: </strong>The interview findings were categorized into three themes: willingness to use mobile health-based parenting interventions, content needs, and functional needs. Some parents expressed strong willingness to use mobile health-based parenting interventions, while others underestimated their need for such programs due to various factors. Content needs included guidance on parent-child communication, family conflict resolution, stress management for children, professional knowledge about the illness, and parental emotional regulation. Functional needs involved personalized content delivery, enabling the transformation of knowledge into skills, and providing timely guidance and feedback.</p><p><strong>Conclusions: </strong>Before implementing mobile health-based parenting interventions, it is crucial to understand parents' willingness and address their specific content and presentation needs. Interventions should deliver personalized and precise parenting knowledge while offering timely guidance and feedback to facilitate the transformation of knowledge into parenting skills.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 9","pages":"1469-1476"},"PeriodicalIF":0.0,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11814391/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beta<b>-</b>amyloid protein regulates miR<b>-</b>15a and activates Bag5 to influence neuronal apoptosis in Alzheimer<b>'</b>s disease.","authors":"Qiong Pan, Xinyu Hu, Ke Guo","doi":"10.11817/j.issn.1672-7347.2024.230439","DOIUrl":"10.11817/j.issn.1672-7347.2024.230439","url":null,"abstract":"<p><strong>Objectives: </strong>The prevalence of Alzheimer's disease (AD) is increasing globally, however its pathogenesis is still unclear. The evidence showed that the progression of AD was closely related to the apoptosis of nerve cells. This study amis to explore the role and specific mechanism of miR-15a and Bag5 in the apoptosis of nerve cells induced by beta-amyloid protein (Aβ) in AD.</p><p><strong>Methods: </strong>The AD rat model was constructed by injecting Aβ42 into SD rat brain and the AD cell model was constructed by treating SH-SY5Y cells with Aβ42. The learning and memory ability of rats was detected by Morris Water Maze. Hematoxylin and eosin (HE) staining was used to detect the pathological changes of brain tissues. Nissl staining was used to detect the changes of cell morphology and number in brain tissues. The upstream miRNA that interacted with Bag5 were screened by bioinformatics analysis. Methyl thiazolyl tetrazolium (MTT) assay was used to detect cell proliferation. Flow cytometry was used to detect the apoptosis rate of cells. Real-time reverse transcription PCR (real-time RT-PCR) was used to detect the mRNA levels of <i>miR-15a</i> and <i>Bag5</i>. Western blotting was used to detect the protein expression levels of Bag5, Bax and Caspase-3. <i>MiR-15a</i> knockdown or overexpression vectors or <i>Bag5</i> knockdown vectors were transfected into AD rat model and AD cell models, respectively. Luciferase reporter assay was used to verify the binding relationship between miR-15a and Bag5.</p><p><strong>Results: </strong>Morris Water Maze, HE staining and Nissl staining showed that the rat model of AD was established successfully, and Aβ could induce neuronal apoptosis and inhibit the expression of miR-15a in AD rats. Compared with normal cells, Aβ treatment significantly increased apoptosis rate and Bag5 expression, and weakened cell proliferation and miR-15a (all <i>P</i><0.01). Overexpression of miR-15a further enhanced the effect of Aβ on cell proliferation and apoptosis, while knockdown of miR-15a expression had the opposite effect (all <i>P</i><0.01). Luciferase reporter assay confirmed that there was a negative targeting relationship between miR-15a and Bag5. Compared with Bag5 knockdown alone, the co-transfection of miR-15a inhibitor and si-Bag5 significantly increased the cell proliferation ability and mRNA and protein levels of Bag5, and significantly reduced the cell apoptosis rate and the expression of Bax and Caspase-3, animal studies have also shown consistent results (all <i>P</i><0.01).</p><p><strong>Conclusions: </strong>Aβ can inhibit the expression of miR-15a, thereby inducing the expression of Bag5 and activating the protective mechanism of Bag5 against Aβ induced apoptosis.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 7","pages":"1109-1119"},"PeriodicalIF":0.0,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11495979/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142956168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prenatal diagnosis and genetic analysis of novel missense mutation in <i>FVIII</i> gene.","authors":"Yarong Guo, Lin Liu, Bin Mao, Zehui Xie, Qimin Tian, Chuan Zhang, Xiaoling Ma","doi":"10.11817/j.issn.1672-7347.2024.230520","DOIUrl":"10.11817/j.issn.1672-7347.2024.230520","url":null,"abstract":"<p><p>Hemophilia A (HA) is an X-linked recessive hereditary bleeding disorder characterized by recurrent bleeding into muscles, deep tissues, and joint cavities, which may result in varying degrees of disability. Currently, there is no cure for this disease. In this study, the First Hospital of Lanzhou University conducted prenatal diagnosis and family genetic testing on a female carrier of the coagulation factor VIII (<i>FVIII</i>) gene mutation c.5878 C>G in exon 18, classified as a variant of unknown significance. The analysis confirmed that the c.5878 C>G mutation is the pathogenic mutation in the HA family. The proband and her mother were both heterozygous carriers, while her brother was a hemizygous patient. This mutation has not been previously reported and represents a novel pathogenic missense mutation. Family genetic analysis supported the X-linked recessive inheritance pattern. By performing preimplantation genetic testing for monogenic diseases (PGT-M), embryos were screened for the mutation, allowing the carrier to successfully give birth to a healthy child. Screening for <i>FVIII</i> gene mutations to identify mutation types and loci, combined with preconception, preimplantation, and prenatal genetic diagnosis, is critical for reducing adverse pregnancy outcomes and preventing the birth of HA-affected offspring.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 9","pages":"1523-1530"},"PeriodicalIF":0.0,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11814398/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of protein post-translational modifications in unexplained recurrent pregnancy loss.","authors":"Qianqian Gao, Jiajie Ren, Dingren Niu, Luqin Guo, Xiaoling Feng","doi":"10.11817/j.issn.1672-7347.2024.240365","DOIUrl":"10.11817/j.issn.1672-7347.2024.240365","url":null,"abstract":"<p><p>Unexplained recurrent pregnancy loss (URPL) is a complex pathological condition that poses significant challenges for women of childbearing age. As a critical component of epigenetics, post-translational modifications (PTMs) not only regulate protein expression and enhance its diversity but also modulate interactions between proteins and other molecules. PTMs play an essential role in the onset and progression of URPL. For example, methylation modifications can hinder decidualization and regulate trophoblast migration and invasion. Phosphorylation modifications can balance regulatory T cells (Treg)/T helper 17 cells (Th17), polarize macrophages, alter dendritic cell populations, and promote trophoblast apoptosis. Acetylation modifications can induce trophoblasts autophagy and suppress M2 macrophage polarization. Ubiquitination modifications can modulate trophoblast migration and invasion and disrupt the immune microenvironment. Glycosylation modifications can inhibit trophoblast migration and invasion, while lactylation modifications maintain endometrial receptivity. The role of PTMs in URPL highlights their potential as a research entry point and therapeutic target for this condition.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 9","pages":"1495-1502"},"PeriodicalIF":0.0,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11814392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between obesity and Alzheimer<b>'</b>s disease and the mechanisms.","authors":"Tenglin Zhang, Ping Wang, Ruonan Li, Ying Wang, Shuxun Yan","doi":"10.11817/j.issn.1672-7347.2024.240025","DOIUrl":"10.11817/j.issn.1672-7347.2024.240025","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is a progressive central neurodegenerative disorder with an insidious onset. With global aging, the incidence and mortality of AD have been steadily increasing, yet effective treatments remain elusive. Obesity, characterized by excessive or abnormal fat accumulation, is a complex metabolic disorder and a confirmed risk factor for numerous diseases. Both obesity and AD have become major public health concerns, posing significant threats to human health and economic development. Studies have revealed a strong correlation between obesity and AD, with multiple contributing factors, including metabolic abnormalities of endocrine factors, inflammatory responses, and genetic interactions. Exploring the correlation and mechanisms between obesity and AD provides important insights and new strategies for the prevention and treatment of AD.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 7","pages":"1052-1061"},"PeriodicalIF":0.0,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11495975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142956231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of apoptosis<b>-</b>related long non<b>-</b>coding RNAs expression profiles in patient with cervical intraepithelial neoplasia 3.","authors":"Zhaoyang Liu, Lingli Luo, Xinyi Wu, Bingqi Wang, Min Wang, Xianping Li","doi":"10.11817/j.issn.1672-7347.2024.230302","DOIUrl":"10.11817/j.issn.1672-7347.2024.230302","url":null,"abstract":"<p><strong>Objectives: </strong>Long non-coding RNAs (lncRNAs) play an essential role in cancer biology. Cervical intraepithelial neoplasia grade 3 (CIN3) is the most severe precancerous lesion of cervical cancer. However, the mechanism of multiple lncRNAs in CIN3 has not been studied in-depth and is worth exploring. This study aims to summarize the lncRNA expression profile in CIN 3 and screen for lncRNAs with potential oncogenic effects.</p><p><strong>Methods: </strong>To further clarify the role of lncRNAs in the development of CIN3, this study collected cancer tissue and para-cancer tissue specimens from three CIN3 patients. The RNA sequencing (RNA-Seq) analysis was used to construct expression profiles of lncRNAs and confirmed by real-time reverse transcription PCR (real-time RT-PCR) methods. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were performed with computational methods. We obtained their possible target genes through the cis- and trans-regulatory analysis of the lncRNAs. In addition, this study predicted new transcripts and lncRNAs by Cufflinks analysis and coding potential identification (Coding Potential Calculator) analysis to discover new tumor regulatory molecules.</p><p><strong>Results: </strong>The differential expressions of 1 555 genes, 1 562 mRNAs, 241 ncRNAs, and 6 616 new predicted-lncRNAs were found. The most prominently up-regulated and down-regulated lncRNAs were NR_145433.1 and ENST00000513672.1, respectively. KEGG analysis revealed that lncRNA-targeted genes were closely related to apoptosis, metabolic, p53 signaling pathway, and other cancer-related pathways. The real-time RT-PCR validation results of 5 apoptosis-related lncRNAs (NR_145433.1, ENST00000510610.2, UC001Kfo, ENST00000602964.1, and NR_123733.1) in cervical cancer were consistent with the sequencing results.</p><p><strong>Conclusions: </strong>This study provides a comprehensive expression profile of lncRNAs in CIN3 patients, particularly the apoptosis-related expression profile of lncRNAs, and provides direction and clues for the study of cervical cancer and the search for potential therapeutic targets.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 7","pages":"1095-1108"},"PeriodicalIF":0.0,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11495978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142956351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Childhood obesity and central precocious puberty.","authors":"Ruonan Li, Xin Shang, Tenglin Zhang, Shuxun Yan","doi":"10.11817/j.issn.1672-7347.2024.240280","DOIUrl":"10.11817/j.issn.1672-7347.2024.240280","url":null,"abstract":"<p><p>Central precocious puberty (CPP) is an endocrine disorder in children caused by the early activation of the hypothalamic-pituitary-gonadal axis (HPGA), leading to elevated gonadotropin-releasing hormone (GnRH), which triggers the development of gonads and the secretion of sex hormones. This eventually results in the development of internal and external genitalia and secondary sexual characteristics. CPP significantly affects the physical and mental health of children and may increase the risk of various adult diseases. The influencing factors and mechanisms of CPP are a central focus of research, and its prevention and treatment remain challenging. Childhood obesity is an important risk factor for CPP, with a complex relationship influenced by endocrine-disrupting chemicals, genetic factors, and epigenetic regulation. The link between the two is primarily related to the regulation of HPGA function by nutritional and metabolic signals. Exploring the relationship between childhood obesity and CPP, along with the potential mechanisms by which obesity induces CPP, can provide theoretical references for identifying new therapeutic targets.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 7","pages":"1034-1041"},"PeriodicalIF":0.0,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11495981/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142956225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotics in reducing obesity by reconfiguring the gut microbiota.","authors":"Yunuo Zhang, Wei Wang","doi":"10.11817/j.issn.1672-7347.2024.240361","DOIUrl":"10.11817/j.issn.1672-7347.2024.240361","url":null,"abstract":"<p><p>Obesity, as a global health crisis, is increasingly linked to intestinal microecology. Probiotics colonise the body, effectively regulating the balance of intestinal flora, while strengthening the intestinal barrier, activating the immune response, releasing beneficial substances, and maintaining micro-ecological balance. This process not only enhances the defence against pathogens, but also reduces the production of inflammatory factors and lowers the level of chronic inflammation. However, the specific process and mechanism by which probiotics influence the intestinal microecology through the immune response, improve metabolic disorders caused by obesity, and participate in weight management are not clear. Through multiple neural pathways including the 'gut-brain axis' and their direct interaction with the intestine, probiotics increase the number of beneficial bacteria in the intestine and inhibit the growth of harmful bacteria, thus effectively restructuring the balance of the intestinal flora. This restructuring of the balance can optimise the intestinal environment and enhance the efficiency of food digestion and nutrient absorption. Probiotics show positive effects on obesity management by regulating the metabolic process and reducing fat accumulation, providing individuals with a new way to control body weight and prevent obesity. Therefore, the application of probiotics is of great significance in promoting gut health and weight management.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 7","pages":"1042-1051"},"PeriodicalIF":0.0,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11495983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142956307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of waist circumference<b>-</b>to<b>-</b>height ratio in assessing adiposity, predicting type 2 diabetes mellitus and other cardiometabolic diseases.","authors":"Xinyi Zhang, Xi Lu, Xiaohui Pan, Sumin Shen, Nanwei Tong","doi":"10.11817/j.issn.1672-7347.2024.240259","DOIUrl":"10.11817/j.issn.1672-7347.2024.240259","url":null,"abstract":"<p><p>Although body mass index (BMI) is widely used as a simple tool to assess obesity, it has certain limitations and inaccuracies. It is known that visceral adipose tissue is closely related to cardiometabolic risks and all-cause mortality; however, precise measurement methods for visceral fat (magnetic resonance imaging and computed tomography) cannot be widely used. Thus, simple but accurate alternatives are valuable. Studies have shown that waist circumference-to-height ratio (WHtR) might be a superior and more accurate variable in assessing central or visceral adiposity as well as predicting risks of diabetes and other cardiometabolic diseases. Furthermore, WHtR cutoff values can be consistent across different races, age, and genders, making it a universal metric worth promoting and applying.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 7","pages":"1062-1072"},"PeriodicalIF":0.0,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11495984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142956459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}