中南大学学报(医学版)最新文献

{"title":"Factors influencing the use of weight management APP among obese patients in Chinese cities in the post-epidemic era.","authors":"Shujuan Qu, Wenguang Yan, Min Zhou, Wei He","doi":"10.11817/j.issn.1672-7347.2024.240052","DOIUrl":"10.11817/j.issn.1672-7347.2024.240052","url":null,"abstract":"<p><strong>Objectives: </strong>In the post-epidemic era, the prevalence of obesity among urban residents in China has risen sharply, with 34.8% of the population being overweight and 14.1% classified as obese. Obesity has become a critical public health challenge. Given the potential of weight management application (APP) in facilitating health management and weight control, this study aims to explore the factors influencing the adoption of weight management APP by obese patients, providing a scientific basis for the effective health management strategies.</p><p><strong>Methods: </strong>From March to October 2023, a randomized sampling method was used to conduct an online survey among 1 728 residents aged 18-65 from six major Chinese cities: Beijing, Shanghai, Guangzhou, Wuhan, Changsha, and Chengdu. A theoretical model extending the technology acceptance model (TAM) was proposed, incorporating psychological variables such as perceived usefulness, perceived ease of use, social influence, perceived risk, health knowledge, and attitude, alongside demographic variables such as gender, age, education level, and residence. Hypothesis testing was conducted using structural equation modeling to analyze the factors influencing the adoption of weight management APP by obese patients.</p><p><strong>Results: </strong>For obese patients, perceived ease of use significantly predicted perceived usefulness (<i>β</i>=0.301, <i>P</i><0.001), social influence (<i>β</i>=0.132, <i>P</i><0.001), and attitude (<i>β</i>=0.266, <i>P</i><0.001). Perceived usefulness significantly predicted social influence (<i>β</i>=0.171, <i>P</i><0.001) and attitude (<i>β</i>=0.499, <i>P</i><0.001). Social influence (<i>β</i>=0.285, <i>P</i><0.001) and knowledge (<i>β</i>=0.078, <i>P</i><0.001) were significant positive predictors of attitude. The negative impact of perceived risk on attitude was not significant (<i>P</i>=0.166), but its negative influence on actual use was significant though weak (<i>β</i>=-0.051, <i>P</i>=0.036). Attitude significantly influenced actual use (<i>β</i>=0.549, <i>P</i><0.001), highlighting that positive user attitudes are a key driver of actual usage behavior. Additionally, demographic variables such as gender, age, education level, and residence significantly moderated the relationships between attitude and actual use. Female users were more likely to translate perceived usefulness into attitudes, whereas male users were more likely to translate attitudes into actual use, characterizing women as \"pragmatists\" and men as \"action-takers\". Younger users (below 35 years) focused on effectiveness, while higher-educated users (college level and above) were more likely to forgo APP usage due to risk concerns, although positive attitudes drove their actual use.</p><p><strong>Conclusions: </strong>Promoting the use of weight management APP in the post-pandemic era is a critical measure to enhance self-management capabilities among obese patients. Understandin","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 10","pages":"1671-1682"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897970/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complement C5/C5a in the diagnosis and treatment of Graves<b>'</b> ophthalmopathy: A Mendelian randomized study.","authors":"Min Zhu, Bingxuan Wu, Changci Chen-Zhao, Haiyan Chen, Wei Xiong","doi":"10.11817/j.issn.1672-7347.2024.240062","DOIUrl":"10.11817/j.issn.1672-7347.2024.240062","url":null,"abstract":"<p><strong>Objectives: </strong>Graves' ophthalmopathy is a complex organ-specific autoimmune disease with an unclear pathogenesis. Complement component 5/5a (C5/C5a), a key element of the component system, may play a significant role in the disease's pathological process. This study aims to investigate the causal relationship between C5/C5a and Graves' ophthalmopathy using Mendelian randomization (MR) to provide new theoretical insights for its diagnosis and treatment.</p><p><strong>Methods: </strong>Utilizing summary data from genome-wide association study (GWAS), C5/C5a was designated as the exposure factor and Graves' ophthalmopathy as the outcome. The causal relationship between C5/C5a and Graves' ophthalmopathy was analyzed, and colocalization analysis was performed to determine the posterior probability of hypothesis (PPH) and verify the genetic association between C5 and Graves' ophthalmopathy.</p><p><strong>Results: </strong>The Wald ratio model showed a significant positive correlation between C5 and Graves' ophthalmopathy (<i>OR</i>=4.109, 95% <i>CI</i> 1.990 to 8.486, <i>P</i><0.001). Similarly, the inverse variance weighted (IVW) model showed a positive correlation between C5a and Graves' ophthalmopathy (<i>OR</i>=2.901, 95% <i>CI</i> 1.225 to 6.869, <i>P</i>=0.015). Colocalization analysis showed that C5 and Graves' ophthalmopathy share a single nucleotide polymorphism (SNP), rs7036980, within the specified genetic window, with a PPH4 value of 0.81 (a value >0.80 indicates high probability).</p><p><strong>Conclusions: </strong>Elevated levels of C5/C5a significantly increase the risk of developing Graves' ophthalmopathy. Targeting complement C5 with inhibitors may effectively reduce the risk of Graves' ophthalmopathy.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 10","pages":"1633-1641"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of the anterior cingulate cortex in sleep regulation.","authors":"Shangtao Zhou, Chengxi Liu, Wenjie Liu, Yan Wang","doi":"10.11817/j.issn.1672-7347.2024.240343","DOIUrl":"10.11817/j.issn.1672-7347.2024.240343","url":null,"abstract":"<p><p>Sleep disorders refer to conditions characterized by abnormal sleep duration and quality, including insomnia, sleep-disordered breathing, and fragmented sleep, and have become one of the major challenges to modern physical and mental health. The anterior cingulate cortex (ACC) is an important component of the limbic system, located between the cingulate sulcus and the callosal sulcus on the medial surface of the cerebral hemispheres, and plays a critical role in regulating autonomic movements, emotions, and pain. It is an important part of the sleep regulation system. In patients with primary insomnia, reduced sleep duration is associated with lower levels of gamma-aminobutyric acid in the ACC, and these patients often exhibit increased ACC volume and altered functional structure. The ACC is recognized as a central region for pain perception and the regulation of negative emotions; it participates in the control of chronic pain and regulates pain-related insomnia via descending projections. Moreover, the ACC is a key area in the pathophysiology of major depressive disorder, where individuals with depression or poor sleep quality show enhanced functional connectivity between the ACC and regions such as the lateral orbitofrontal cortex, posterior cingulate cortex, precuneus, angular gyrus, and temporal cortex. Abnormal functional connectivity within ACC subregions is implicated in anhedonia and impaired sleep quality in patients with major depressive disorder.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 10","pages":"1576-1581"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143616692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Robotic-assisted surgical treatment of complex giant incisional hernias: A report of 3 cases.","authors":"Yandong Xiang, Jiaxuan Xin, Yihui Liu, Bo Yi","doi":"10.11817/j.issn.1672-7347.2024.240293","DOIUrl":"10.11817/j.issn.1672-7347.2024.240293","url":null,"abstract":"<p><p>Incisional hernia of abdominal wall is one of the most common complications following open surgery. Giant incisional hernia of abdominal wall is defined as having a maximum defect diameter >12 cm or a hernia sac volume-to-abdominal cavity volume ratio >20%. The Primary treatment for giant incisional hernia of abdominal wall is surgical repair; however, both open surgery and conventional laparoscopic surgery are associated with significant technical difficulty, high surgical trauma, frequent postoperative complications, and suboptimal outcomes. From May 2021 to November 2023, 3 patients with giant incisional hernia of abdominal wall were treated at the Third Xiangya Hospital of Central South University with robotic-assisted intraperitoneal onlay mesh repair using the da Vinci surgical system. These patients experienced minimal intraoperative bleeding, rapid postoperative recovery, no wound infections or severe complications, and no recurrences. Compared with traditional open and laparoscopic surgeries, robotic-assisted surgery demonstrates unique advantages in the clinical management of giant incisional hernias.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 10","pages":"1700-1705"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897969/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new removable connecting device for MARPE expander with non-common path of insertion.","authors":"Shuiping Wu, Yuhan Xu, Yingjie Li, Wei Zhu, Zhenlong Zhang, Lingling Zhang, Chengri Li, Junjie Chen, Liu Qian, Yusi Li, Xian Cheng, Yanping Liu, Yanqin Lu","doi":"10.11817/j.issn.1672-7347.2024.240283","DOIUrl":"10.11817/j.issn.1672-7347.2024.240283","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;Maxillary transverse deficiency is a common malocclusion frequently observed in orthodontic clinics. Miniscrew-assisted rapid palatal expansion (MARPE) not only produces greater skeletal expansion but also offers advantages such as simple miniscrew implantation without flap elevation, enhanced patient comfort, and an expanded age range and indications for palatal expansion. However, the fixed connection between the expander and the miniscrews makes the expander difficult to remove, significantly hindering its clinical application. This study aims to employ a non-common path of insertion connection device-comprising 3D-printed miniscrew caps and silk fibroin (SF) electrospun membranes-to optimize the clinical operation of non-co-located MARPE in orthodontic expansion.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The removable connection device for non-common path of insertion MARPE consists of 3D-printed miniscrew caps and SF electrospun membranes. First, the miniscrew cap was measured using reverse engineering software (Geomagic Wrap) to generate optimal fit data, and designed with the 3D creation program Cinema4D to form an internal cylinder and an external protective ring, with the design saved as a STL file. The data were then imported into Voxelddance Tango software to design a virtual base scaffold, and the cap was fabricated using a 3D printer. Subsequently, SF and polyethylene oxide (PEO) were electrospun at various mass ratios to form SF electrospun membranes. The morphology was observed using scanning electron microscope (SEM), and Fourier-transform infrared (FTIR) spectroscopy was used to analyze the secondary structural conformation of both the miniscrew cap and the SF electrospun membranes. Tensile and compression tests were conducted to evaluate their mechanical properties, and the sealing performance of the removable connection device (with and without the SF electrospun membrane) was compared. In addition, cell compatibility was assessed by co-culturing the miniscrew caps and SF electrospun membranes with mouse embryonic fibroblasts (NIH 3T3), using a live/dead cell staining kit and the cell counting kit-8 (CCK-8). Finally, a 13-year-old male patient at palatal suture stage D was selected. Pre-implantation cone beam computed tomography (CBCT) measured the palatal soft and hard tissue thickness at the planned insertion site, and 2 miniscrews were implanted at the optimal bone density site between the second premolar and the first molar. An intraoral scan model was used to integrate teeth 13 and 23 into a tooth-bone hybrid-supported expander, which was customized. The miniscrew caps were trial-fitted, and the expander was adjusted until it fitted perfectly without interference. The expander was then bonded, with the SF electrospun membrane wrapped around and covering the miniscrews, and the miniscrew cap applied. Light-cured resin was used to connect the expander and the miniscrew caps. The patient was instructed t","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 10","pages":"1649-1662"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"5<b>-</b>HT1_AR agonist alleviates presurgical prolonged sleep deprivation<b>-</b>induced postsurgical pain in adolescent mice.","authors":"Wei Zhu, Xuan Xu, Rui Xu, Yulin Huang, Zhengliang Ma","doi":"10.11817/j.issn.1672-7347.2024.240499","DOIUrl":"10.11817/j.issn.1672-7347.2024.240499","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;Sleep deprivation (SD) is a risk factor for the development of chronic pain in adolescents, significantly affecting pain management and prognosis; however, the mechanisms by which SD influences postoperative pain outcomes remain unclear. This study aims to investigate the molecular mechanism through which the spinal 5-hydroxytryptamine 1&lt;sub&gt;A&lt;/sub&gt; receptor (5-HT1&lt;sub&gt;A&lt;/sub&gt;R) regulates the excitation/inhibition (E/I) balance in the dorsal horn to modulate postoperative chronic pain induced by SD in adolescent mice.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A pain model combining 4.5 days of SD and a plantar incision was established in adolescent C57BL/6J mice, which were randomly assigned to 4 groups: control (C), SD, hind toe incision (I), and SD combined with hind toe incision (SI). The effects of a single intrathecal injection of the 5-HT1&lt;sub&gt;A&lt;/sub&gt;R agonist 8-hydroxy-2-dipropylamino-tetralin (8-OH-DPAT) and both single and continuous intrathecal injections of the extracellular signal-regulated kinase (ERK) inhibitor U0126 on SD-induced postoperative chronic in mice (SI+8-OH-DPAT group, SI+U0126 group, and SI+Vehicle group) were observed. Paw withdrawal mechanical threshold (PWMT) was measured on the 1st, 3rd, 5th, 7th, 10th, and 14th day before and after model induction. On the 7th day after surgery, immunofluorescence was used to assess 5-HT1&lt;sub&gt;A&lt;/sub&gt;R expression in the spinal dorsal horn, and Western blotting was employed to measure protein expression levels of 5-HT1&lt;sub&gt;A&lt;/sub&gt;R, postsynaptic density protein-95 (PSD95), &lt;i&gt;N&lt;/i&gt;-methyl-D-aspartic acid receptor 1 (NR1), phosphorylated NR1 (p-NR1), vesicular glutamate transporter 1 (VGLUT1), gephyrin, glutamic acid decarboxylase 67 (GAD67), vesicular gamma-aminobutyric acid transporter (VGAT), ERK, p-ERK, calmodulin-dependent protein kinase II (CaMKⅡ), phosphorylated CaMKⅡ (p-CaMKⅡ), cAMP- response element-binding protein (CREB), and phosphorylated CREB (p-CREB) in the dorsal horn.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;1) Behavioral pain analysis. Compared with group C, the PWMT in groups I and SD decreased significantly on the 1st, 3rd, 5th and 7th day after surgery (all &lt;i&gt;P&lt;/i&gt;&lt;0.05), and returned to baseline on the 10th day. Compared with group I, the PWMT in group SI was significantly lower on the 5th, 7th and 10th day (all &lt;i&gt;P&lt;/i&gt;&lt;0.05). The SI+8-OH-DPAT group exhibited higher PWMT values at the 1st, 2nd, and 4th hour postoperatively, as well as on the 3rd, 5th, 7th,10th and 14th day compared to the solvent control group (all &lt;i&gt;P&lt;/i&gt;&lt;0.05). Similarly, both single and continuous intrathecal injections of U0126 resulted in higher PWMT values at 1, 2, and 4 hours after surgery postoperatively and on the 3rd, 5th and 7th day compared to the SI+Vehicle group (all &lt;i&gt;P&lt;/i&gt;&lt;0.05). 2) Immunofluorescence. On the 7th day after surgery, group SI showed decreased 5-HT1&lt;sub&gt;A&lt;/sub&gt;R expression in the spinal dorsal horn, while a single intrathecal injection of 8-OH-DPAT upr","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 10","pages":"1543-1555"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agreement analysis of subjective and objective sleep quality in adolescents with mood disorders.","authors":"Tengwei Chen, Lu Niu, Jiaxin Zhu, Dan Qiu, Chengcheng Zhang, Yuedong Chen, Honghui Zhang, Xiaofei Hou, Haojuan Tao, Xiyuan Chen, Yarong Ma, Kangguang Lin, Liang Zhou","doi":"10.11817/j.issn.1672-7347.2024.240346","DOIUrl":"10.11817/j.issn.1672-7347.2024.240346","url":null,"abstract":"<p><strong>Objectives: </strong>Accurate assessment of sleep quality is crucial for understanding sleep problems and their impact on health. This study analyzed the agreement between subjective sleep assessments and objective sleep monitoring in adolescents with mood disorders, aiming to provide a reliable methodological foundation for related research.</p><p><strong>Methods: </strong>Adolescents with mood disorders were recruited from psychiatric outpatient clinics of three domestic hospitals. A consensus sleep diary and an actigraph (activity tracker) were used to monitor sleep for 14 days. The differences between subjective and objective measurements were compared using the Wilcoxon signed-rank test, while Bland-Altman plots and intraclass correlation coefficients (ICC), described their agreement. Spearman's rank correlation coefficients were used to assess their correlation, and mixed-effects models analyzed factors influencing the differences between subjective and objective measurements.</p><p><strong>Results: </strong>Significant differences were observed between subjective and objective measures for the number of awakenings after sleep onset (NWAK), waking after sleep onset (WASO), total sleep time (TST), and wake-up time (<i>P</i><0.001); however, the difference in sleep onset time was not statistically significant (<i>P</i>=0.283). Subjective and objective measurements of sleep onset (ICC=0.821, <i>r</i>=0.838) and wake-up time (ICC=0.821, <i>r</i>=0.836) demonstrated good agreement and correlation; TST showed moderate agreement (ICC=0.640) and correlation (<i>r</i>=0.682); NWAK (ICC=0.210, <i>r</i>=0.276) and WASO (ICC=0.358, <i>r</i>=0.365) exhibited poor and correlation. In the Bland-Altman plots, most data points for sleep onset, wake-up time, and TST were uniformly distributed within the 95% limits of agreement (LoA). The differences between subjective and objective measurements for WASO and NWAK increased with higher average values. However, the 95% LoA of the differences between subjective and objective measurements of all the above indicators exceeded the acceptable ranges of the corresponding indicators, indicating poor agreement. Baseline depression levels were associated with the differences between subjective and objective measurements of NWAK (<i>β</i>=0.034, <i>P</i><0.05), TST (<i>β</i>=2.617, <i>P</i><0.01), and sleep onset (<i>β</i>=1.454, <i>P</i><0.05), while sleep quality scores were associated with the difference in WASO (<i>β</i>=0.051, <i>P</i><0.01).</p><p><strong>Conclusions: </strong>Considerable discrepancies remain between subjective sleep diaries and sleep monitoring data from actigraphy (activity tracker). Future research should further investigate factors influencing the discrepancies between subjective and objective measurements and refine measurement methods to obtain more reliable sleep data.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 10","pages":"1566-1575"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and localization of HBsAg in corneas from HBsAg<b>-</b>seropositive donors preserved in medium<b>-</b>term preservation solution and glycerol.","authors":"Hong Ren, Ziyi Cai, Jiaqi DU, Gaoyang Li, Shuyu Hu, Sha Ouyang, Hanhan Liu, Zhihong Deng","doi":"10.11817/j.issn.1672-7347.2024.230564","DOIUrl":"10.11817/j.issn.1672-7347.2024.230564","url":null,"abstract":"<p><strong>Objectives: </strong>Due to the severe shortage of donor corneas for transplantation in China, corneal component transplantation may expand the available donor pool. This study aims to evaluate the safety and feasibility of corneal component transplantation by examining the distribution of hepatitis B surface antigen (HBsAg) in corneas from HBsAg-seropositive donors under different storage media.</p><p><strong>Methods: </strong>Ten corneas (from 6 donors) donated between December 2019 and March 2021 and stored at the Eye Bank of Xiangya Third Hospital, Central South University, were analyzed. Serum HBsAg levels were quantified using enzyme-linked immunosorbent assay (ELISA), while HBV DNA titers in donor blood were measured via polymerase chain reaction (PCR). Immunofluorescence staining was employed to detect HBsAg expression and its localization within corneal layers. Correlations between blood HBV DNA titers and HBsAg fluorescence intensity in corneal tissues were statistically evaluated.</p><p><strong>Results: </strong>Four corneas from 2 HBsAg-seropositive donors and 1 cornea from 1 HBsAg-seronegative donor were preserved in medium-term preservation solution for <2 weeks. Two corneas from 1 HBsAg-seropositive donor and 2 corneas from another HBsAg-seropositive donor were preserved in glycerol for 1 month and 1 year, respectively. One HBsAg-seronegative donor cornea was stored in glycerol for 1 year. Immunofluorescence staining revealed HBsAg-positive signals in the epithelium of 2 corneas and the endothelium of 1 cornea preserved in medium-term preservation solution (<2 weeks), as well as in the epithelium of 1 cornea stored in glycerol long-term (1 year). Semi-quantitative analysis demonstrated statistically significant differences in HBsAg fluorescence intensity between the corneal epithelium and endothelium (<i>P</i>=0.012), while no significant variations were observed across preservation methods or donors (<i>P</i>>0.05). A positive correlation was identified between blood HBV DNA titers and HBsAg expression in the corneal epithelium (<i>R</i>²=0.707, <i>P</i>=0.036).</p><p><strong>Conclusions: </strong>HBsAg-positive expression persists in the epithelium or endothelium of donor corneas from HBsAg-seropositive individuals, regardless of preservation in medium-term preservation solution or glycerol. However, no HBsAg expression was detected in the corneal stroma. Higher blood HBV DNA titers correlate with increased likelihood of HBsAg positivity in corneal tissues. This study lays the groundwork for further investigation into the potential use of HBsAg-positive donor corneas in lamellar keratoplasty.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 10","pages":"1642-1648"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of cognitive impairment associated with cerebral infarction.","authors":"Qing Yin, Li Yang","doi":"10.11817/j.issn.1672-7347.2024.240213","DOIUrl":"10.11817/j.issn.1672-7347.2024.240213","url":null,"abstract":"<p><p>Cerebral infarction is a common type of stroke with high incidence and disability rates, and most patients experience varying degrees of cognitive impairment. The manifestations and severity of post-infarction cognitive impairment are influenced by multiple interacting factors, and its pathophysiological mechanisms are highly complex, involving pericyte degeneration, excessive generation of reactive oxygen species (ROS), overproduction of glutamate, and overactivation of autophagy. After cerebral infarction, abnormal pericyte function activates neuroinflammation and facilitates the entry of inflammatory mediators into the brain; detachment of pericytes from blood vessels disrupts the integrity of the blood-brain barrier. Although angiogenesis and glial scar formation may alleviate injury, excessive scarring can inhibit neuronal regeneration. Excessive ROS trigger oxidative stress, leading to mitochondrial dysfunction, ferroptosis, and suppression of endothelial nitric oxide synthase/nitric oxide signaling, thereby damaging neurons. An excessive surge in glutamate release, coupled with insufficient clearance, results in its accumulation in the intercellular space, leading to excitotoxicity; the influx of calcium ions subsequently activates proteases and apoptotic pathways, causing neuronal death. Overactivation of autophagy alters lysosomal membrane permeability and results in leakage of lysosomal enzymes; oligodendrocyte necrosis then leads to severe demyelination, further exacerbating brain injury, although promoting the autophagic clearance of damaged mitochondria can ameliorate cognitive deficits arising from mitochondrial dysfunction.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 10","pages":"1692-1699"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blue rubber bleb nevus syndrome: A case report and literature review.","authors":"Dandan Yuan, Yan Xie, Qi Liang","doi":"10.11817/j.issn.1672-7347.2024.240163","DOIUrl":"10.11817/j.issn.1672-7347.2024.240163","url":null,"abstract":"<p><p>Blue rubber bleb nevus syndrome (BRBNS) is a rare congenital clinical syndrome characterized by venous malformations in multiple organs, including the skin, gastrointestinal tract, liver, and lungs. In June 2022, Third Xiangya Hospital of Central South University admitted a rare case of BRBNS. The patient was hospitalized due to abdominal distension and a history of recurrent hematochezia. Abdominal imaging revealed vascular lesions in the liver and intestines, while colonoscopy demonstrated multiple blue vascular tumors. After symptomatic treatment and sirolimus therapy, the patient's symptoms were alleviated. Given the rarity of BRBNS cases, this report helps deepen the understanding of the clinical manifestations and diagnosis of BRBNS.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 10","pages":"1706-1710"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}