中南大学学报(医学版)最新文献

{"title":"Role of m⁶A RNA methylation in renal resident cell injury.","authors":"Zixia Zhao, Chen Zhang, Si Wu, Junjun Luan, Hua Zhou","doi":"10.11817/j.issn.1672-7347.2024.230600","DOIUrl":"10.11817/j.issn.1672-7347.2024.230600","url":null,"abstract":"<p><p>RNA methylation modification is a highly dynamic and reversible epigenetic regulatory mechanism, primarily controlled by 3 types of factors: Methyltransferases, demethylases, and methylation reader proteins. N⁶-methyladenosine (m⁶A) methylation is the most common form of RNA methylation, and dysregulation of this process may lead to the development of various diseases. Renal diseases have drawn considerable attention owing to their high incidence, poor prognosis, and substantial socioeconomic burden. Renal resident cell injury plays a crucial role in the onset and progression of various kidney diseases. Understanding the mechanisms underlying renal resident cell injury is essential for advancing the prevention and treatment of kidney diseases. Recent studies have revealed that RNA m⁶A methylation plays a critical role in renal resident cell injury, highlighting its potential as a novel therapeutic target for kidney disease treatment.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 11","pages":"1757-1768"},"PeriodicalIF":0.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143774355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of transurethral plasmakinetic resection of the prostate using a small-caliber resectoscope for benign prostatic hyperplasia with mild urethral stricture.","authors":"Zhiwei Zhu, Zhibiao Qing, Junhuan He, Xuecheng Wu, Wuxiong Yuan, Yixing Duan, Yuanwei Li, Mingqiang Zeng","doi":"10.11817/j.issn.1672-7347.2024.240392","DOIUrl":"10.11817/j.issn.1672-7347.2024.240392","url":null,"abstract":"<p><strong>Objectives: </strong>The conventional Fr26 resectoscope is difficult to use in patients with benign prostatic hyperplasia (BPH) complicated by urethral stricture. This study aims to evaluate the safety and efficacy of transurethral plasmakinetic resection of the prostate (PKRP) using a small-caliber (Fr18.5) plasmakinetic resectoscope combined with urethral dilation in patients with BPH and mild urethral stricture.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 37 patients with BPH and mild urethral stricture treated at the Department of Urology, Hunan Provincial People's Hospital from January 2023 to December 2023. All patients underwent PKRP with a small-caliber plasmakinetic resectoscope, followed by routine placement of a Fr20 three-way Foley catheter for continuous bladder irrigation. International Prostate Symptom Score (IPSS), maximum urinary flow rate (<i>Q</i>_max), post-voiding residual urine volume (PVR), and Quality of Life (QOL) scores were compared before and after surgery. Perioperative indicators (intraoperative bleeding, operative time, postoperative catheterization time, and postoperative hospital stay) and complications were recorded.</p><p><strong>Results: </strong>The median age was 69 years, and the median duration of voiding difficulty was 36 months. Median total prostate specific antigen (T-PSA) was 2.095 ng/mL, free prostate specific antigen (F-PSA) 0.561 ng/mL, and F/T ratio 0.3. Median prostate diameter was 48 mm and volume 41 mL. All 37 surgeries were completed successfully: 11 had external meatal stricture, 19 had mild anterior urethral stricture, and 7 had mild posterior urethral stricture (1 patient with a 1 cm pseudo-blind tract near the membranous urethral). Operative time was (2.4±0.7) hours, blood loss was (40±29) mL, median catheterization duration was 7 days, and median hospital stay was 7 days. No cases of postoperative urinary incontinence, recurrent hematuria, or sepsis occurred, and patients were satisfied with the surgical outcome. At 3 to 6 months follow-up, IPSS, <i>Q</i>_max, PVR, and QOL scores significantly improved compared to preoperative levels (all <i>P</i><0.01), with no cases of urethral stricture progression or new-onset stricture.</p><p><strong>Conclusions: </strong>PKRP using a small-caliber plasmakinetic resectoscope is safe and effective for treating BPH with mild urethral stricture. It offers advantages such as minimal trauma, rapid postoperative recovery, and a lower risk recovery, and a lower risk of aggravating urethral injury.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 11","pages":"1751-1756"},"PeriodicalIF":0.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143774512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress in research and development of biodegradable metallic vascular stents.","authors":"Yan Yang, Zhenfeng Zhang, Junwei Wang, Keyun Fu, Dongyang Li, Hao He, Chang Shu","doi":"10.11817/j.issn.1672-7347.2024.230514","DOIUrl":"10.11817/j.issn.1672-7347.2024.230514","url":null,"abstract":"<p><p>Vascular stents are an essential tool in cardiovascular interventional therapy, and their demand is growing with the increasing incidence of cardiovascular diseases. Compared with permanent stents, which are prone to in-stent restenosis, and drug-eluting stents, which may cause late stent thrombosis, biodegradable stents offer advantages. After providing early radial support to prevent elastic recoil, biodegradable stents gradually degrade, allowing the vessel to regain its natural physiological contractility and undergo positive remodeling. A review of the current mainstream biodegradable metal stents, magnesium-based, iron-based, and zinc-based alloys, shows promising findings in both preclinical and clinical research. Magnesium-based stents exhibit good operability and low thrombosis rates, but their limitations include rapid degradation, hydrogen evolution, and significant pH changes in the microenvironment. Iron-based stents demonstrate excellent mechanical strength, formability, biocompatibility, and hemocompatibility, but their slow corrosion rate hampers broader clinical application; accelerating degradation remains key. Zinc-based alloys have a moderate degradation rate but relatively low mechanical strength; enhancing stent strength by alloying with other elements is the main improvement direction for zinc-based stents.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 11","pages":"1861-1868"},"PeriodicalIF":0.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964820/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143773672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential value of B7-H3 in sepsis diagnosis and prognosis: A Mendelian randomization study.","authors":"Mingjun Guo, Zhihui He","doi":"10.11817/j.issn.1672-7347.2024.240139","DOIUrl":"10.11817/j.issn.1672-7347.2024.240139","url":null,"abstract":"<p><strong>Objectives: </strong>Sepsis remains a major global health challenge, yet specific diagnostic biomarkers are still lacking. This study aims to investigate the causal relationship between B7 homologue 3 (B7-H3) and sepsis susceptibility, severity, and clinical outcomes using Mendelian randomization (MR) analysis, in order to evaluate its potential as a biomarker.</p><p><strong>Methods: </strong>Genetic data related to sepsis (including overall sepsis, sepsis-related mortality with 28 days, severe sepsis, and severe sepsis with 28-day mortality) were extracted from genome-wide association study (GWAS) datasets. Single nucleotide polymorphisms (SNPs) associated with B7-H3 were selected as instrumental variables. The inverse-variance weighted (IVW) was used as the primary approach for causal effect estimation, while weighted median (WME) and MR-Egger regression served as supplementary methods. Additionally, a constrained maximum likelihood-model average (cML-MA) approach was employed to enhance the reliability of causal effect estimation. Cochran's <i>Q</i> test was conducted to assess heterogeneity, and MR-PRESSO along with the MR-Egger intercept method were used to detect horizontal pleiotropy. Sensitivity analyses were performed using the leave-one-out method. A reverse MR analysis was performed with sepsis as the exposure and B7-H3 as the outcome to exclude potential reverse causation.</p><p><strong>Results: </strong>IVW analysis indicated a significant positive causal association between B7-H3 and sepsis susceptibility, severity, and clinical outcomes. A genetically predicted 1-standard deviation (SD) increase in B7-H3 levels was associated with a 10.4% increased risk of sepsis (<i>OR</i>=1.104, 95% <i>CI</i> 1.021 to 1.194, <i>P</i>=0.013), a 26.2% increased risk of sepsis-related 28-day mortality (<i>OR</i>=1.262, 95% <i>CI</i> 1.078 to 1.476, <i>P</i>=0.004), a 22.3% increased risk of severe sepsis (<i>OR</i>=1.223, 95% <i>CI</i> 1.023 to 1.463, <i>P</i>=0.027), and a 60.2% increased risk of severe sepsis with 28-day mortality (<i>OR</i>=1.602, 95% <i>CI</i> 1.119 to 2.294, <i>P</i>=0.010). The causal effect direction remained consistent across IVW, WME, MR-Egger, and cML-MA analyses, reinforcing the robustness and reliability of the results. Cochran's <i>Q</i> test showed no heterogeneity (<i>P</i>>0.05), while MR-PRESSO and MR-Egger intercept tests indicated no evidence of horizontal pleiotropy (both <i>P</i>>0.05). The leave-one-out analysis showed that removing individual SNPs did not significantly alter the causal estimates. Reverse MR analysis showed no causal association between sepsis and B7-H3.</p><p><strong>Conclusions: </strong>B7-H3 may serve as an important biomarker for sepsis, as it is closely associated with sepsis susceptibility, severity, and clinical outcomes.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 11","pages":"1790-1798"},"PeriodicalIF":0.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11964807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143773565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro study of a siRNA delivery liposome constructed with an ionizable cationic lipid.","authors":"Dun Hu, Junna Zou, Shengdan Nie, Yan Wang, Shan Wang","doi":"10.11817/j.issn.1672-7347.2024.230247","DOIUrl":"10.11817/j.issn.1672-7347.2024.230247","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;Small interfering RNA (siRNA) can silence disease-related genes through sequence-specific RNA interference (RNAi). Cationic lipid-based liposomes effectively deliver nucleic acids into the cytoplasm but often exhibit significant toxicity. This study aims to synthesize a novel ionizable lipid, Nε-laruoyl-lysine amide (LKA), from natural amino acids, constructed LKA-based liposomes, and perform physicochemical characterization and cell-based experiments to systematically evaluate the potential of these ionizable lipid-based liposomes for nucleic acid delivery.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;LKA was chemically synthesized and characterized by hydrogen nuclear magnetic resonance (NMR) and mass spectrometry (MS). Paper electrophoresis was used to evaluate the pH-responsive changes in the lipid's net charge. Liposomes without payload (LKA-LP), with signal transducer and activator of transcription 3-siRNA (STAT3-siRNA) (LKA-LP@STAT3-siRNA), or with Nile Red (LKA-LP@Nile Red) were prepared by the ethanol injection method. The particle size and morphology of LKA-LP@STAT3-siRNA were measured by laser particle size analyzer and scanning electron microscope, while agarose gel electrophoresis determined the encapsulation efficiency of siRNA. Uptake of the liposomes by human cervical cancer HeLa cells and mouse embryonic fibroblast 3T3 cells was assessed using LKA-LP@Nile Red. Lysosome escape capabilities in human lung adenocarcinoma A549 cells were evaluated by labeling STAT3-siRNA with Cyanine 5 (Cy5) and using a green lysosomal probe. &lt;i&gt;STAT3&lt;/i&gt; gene silencing was assessed by real-time fluorescence quantitative PCR, and cell viability was determined using cell counting kit-8 (CCK-8).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Hydrogen NMR and MS confirmed the successful synthesis of LKA. Paper electrophoresis demonstrated an increase in LKA's positive charge as pH shifted from 7.4 to 5.5. The LKA-LP@STAT3-siRNA liposomes had a near-spherical morphology with a uniform size of (164.1±3.27) nm, polydispersity index (PDI) of 0.174±0.029, remaining stable for up to 7 days, and a siRNA encapsulation efficiency of (64.6±2.8)%. Cell uptake studies revealed increased uptake of LKA-LP at pH 5.0 compared with pH 7.4, with HeLa cells showing a more pronounced uptake than 3T3 cells. Lysosomal escape experiments showed 42.34% colocalization with lysosomes, indicating successful escape. Gene silencing assays demonstrated a significant decrease (&lt;i&gt;P&lt;/i&gt;&lt;0.01) in &lt;i&gt;STAT3&lt;/i&gt; mRNA expression in HeLa cells treated with 50 or 100 nmol/L LKA-LP@STAT3-siRNA. Cytotoxicity assays showed these concentrations induced a markedly greater reduction in HeLa cell viability than in 3T3 cells (&lt;i&gt;P&lt;/i&gt;&lt;0.01).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;LKA, an ionizable cationic lipid, can form LKA-LP liposomes for siRNA delivery. The system successfully achieved gene silencing at the cellular level, showing specific cytotoxicity to HeLa cells, providing a solid foundatio","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 10","pages":"1591-1600"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of exosomes in regulating ferroptosis of tumor cells.","authors":"Ruixue Xu, Yu Wang","doi":"10.11817/j.issn.1672-7347.2024.230595","DOIUrl":"10.11817/j.issn.1672-7347.2024.230595","url":null,"abstract":"<p><p>Exosomes are nanoscale extracellular vesicles widely present in various body fluids. They carry a variety of substances, including proteins, lipids, and nucleic acids, and play significant roles in the body by participating in immune regulation, intercellular signal transduction, and the transport of proteins and nucleic acids. Exosomes can regulate tumor development and drug resistance by modulating ferroptosis. Leveraging the delivery capabilities of exosomes to modulate ferroptosis provides new ideas and greater possibilities for clinical anti-tumor therapies.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 10","pages":"1683-1691"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on the clinical subtyping of obstructive sleep apnea hypopnea syndrome.","authors":"Li Gao, Yating Peng, Ruoyun Ouyang","doi":"10.11817/j.issn.1672-7347.2024.240252","DOIUrl":"10.11817/j.issn.1672-7347.2024.240252","url":null,"abstract":"<p><p>Obstructive sleep apnea hypopnea syndrome (OSAHS) is a common sleep-disordered breathing condition that exhibits a notable degree of heterogeneity, a feature not fully considered in current diagnostic and therapeutic strategies. This article reviews and analyzes research progress in the subtyping of OSAHS from multiple perspectives, including clinical feature-based subtyping, comorbidity-based subtyping, polysomnography (PSG) parameter-based subtyping, and other classification approaches. Existing studies have identified common subtypes based on clinical features and clarified the characteristics of different subgroups in comorbidity-based classifications; the rich data provided by PSG have helped optimize the classification of OSAHS; and multi-dimensional clustering has provided a more precise basis for individualized treatment. Although these studies have deepened the understanding of the heterogeneity of OSAHS, challenges such as significant differences among subtypes and insufficient evidence for alternative therapies remain. Future research should focus on identifying biomarkers and elucidating the underlying pathophysiological mechanisms to advance the development of precision treatments.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 10","pages":"1582-1590"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cystatin C is associated with osteoporosis and fractures: An observational study based on Mendelian randomization analysis.","authors":"Wenhui Wang, Han Wang, Shufeng Lei, Pei He","doi":"10.11817/j.issn.1672-7347.2024.240147","DOIUrl":"10.11817/j.issn.1672-7347.2024.240147","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;Osteoporosis is characterized by decreased bone mass and damaged bone microstructure, often leading to fragility fractures. Low bone mineral density is a key risk factor for fractures. Serum cystatin C (CysC), an endogenous marker of glomerular filtration rate, is negatively correlated with bone mineral density and may be a potential risk factor for osteoporosis. This study aims to investigate the association and potential pathogenic mechanisms between CysC and osteoporosis and fractures in the general population by combining cohort analysis and Mendelian randomization (MR) analysis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Large-scale prospective cohort data from the UK Biobank and summary statistics from genome-wide association study (GWAS) in European populations were utilized, with strict exclusion criteria applied (excluding non-white individuals, those with thyroid diseases, gastrointestinal dysfunction, kidney diseases, rheumatoid diseases, malignant tumors, chronic infections or inflammatory diseases, diabetes, hypertension, and individuals taking medications that affect bone metabolism). Multivariable linear regression, logistic regression, and Cox proportional hazards models were used to analyze the relationship between CysC and bone mineral density, osteoporosis, and fracture risk. All analyses were performed using three sequential models to adjust for confounding factors: Model 1 adjusted for demographic characteristics and lifestyle factors; Model 2 further adjusted for renal function based on Model 1; and Model 3 further adjusted for physical activity based on Model 2. Restricted cubic spline models were used to explore non-linear relationships, and MR analysis was conducted to assess the causal associations between CysC and osteoporosis and fractures.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Multivariate analysis showed that after adjusting for basic variables (Model 1), there was no correlation between CysC and estimated bone mineral density (eBMD) in the overall study population; however, when stratified by gender, both males and females exhibited a significant negative correlation (&lt;i&gt;P&lt;/i&gt;&lt;0.001). After further adjustment for renal function (Model 2) and physical activity level (Model 3), CysC became negatively correlated with eBMD in the overall population (&lt;i&gt;P&lt;/i&gt;&lt;0.001). Moreover, multivariable logistic regression consistently demonstrated that CysC concentration was significantly positively associated with osteoporosis risk (&lt;i&gt;P&lt;/i&gt;&lt;0.01), and this association remained stable across all models. In all populations and models, multivariate Cox regression analysis indicated that subjects in the highest quartile (Q4) of CysC had a significantly increased risk of developing osteoporosis (&lt;i&gt;P&lt;/i&gt;&lt;0.001). In the overall population, the positive association between Q4 CysC levels and fractures was observed only in Models 2 and 3, with a hazard ratio of 1.118 (both &lt;i&gt;P&lt;/i&gt;&lt;0.001); however, after gender stratificati","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 10","pages":"1622-1632"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A cross<b>-</b>sectional study on healthcare workers<b>'</b> sleep and psychological resilience.","authors":"Yuexin Zhang, Hongfei Mo, Jingqiong Tang, Zhiling Feng, Mengqiang Yu","doi":"10.11817/j.issn.1672-7347.2024.240137","DOIUrl":"10.11817/j.issn.1672-7347.2024.240137","url":null,"abstract":"<p><strong>Objectives: </strong>Healthcare workers, as a high-stress professional group, face long-term high-intensity workloads and complex medical environments, resulting in increasingly prominent mental health issues. In particular, the widespread presence of anxiety symptoms and somatic pain has become a major factor affecting both the quality of care and the career development of healthcare workers. This study aims to investigate the mediating and moderating roles of psychological resilience and sleep in the relationship between somatic pain and anxiety among healthcare workers.</p><p><strong>Methods: </strong>A cross-sectional questionnaire survey was conducted among 1 661 healthcare workers. The instruments used included the Generalized Anxiety Disorder-7 (GAD-7), item 3 from the Patient Health Questionnaire-9 (PHQ-9), the 10-item Connor-Davidson Resilience Scale (CD-RISC-10) for psychological resilience, and the Visual Analogue Scale (VAS) for assessing anxiety, sleep disturbance, psychological resilience, and somatic pain.</p><p><strong>Results: </strong>The detection rate of anxiety symptoms among healthcare workers was 38.95%. Psychological resilience was significantly negatively correlated with anxiety symptoms (<i>r</i>=-0.451, <i>P</i><0.01), sleep disturbance (<i>r</i>=-0.313, <i>P</i><0.01), and somatic pain (<i>r</i>=-0.214, <i>P</i><0.01). Moreover, psychological resilience partially mediated the relationship between somatic pain and anxiety (<i>β</i>=-0.103, <i>P</i><0.01), and sleep quality moderated the latter part of the mediation model (\"somatic pain-psychological resilience-anxiety\").</p><p><strong>Conclusions: </strong>Under high-intensity workloads, healthcare workers generally experience severe anxiety symptoms. Psychological resilience plays an important protective mediating role in their mental health, and sleep quality serves as a moderator in this relationship. Enhancing healthcare workers' psychological resilience and improving their sleep may promote both their physical and mental well-being.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 10","pages":"1556-1565"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic antibacterial effects of pinaverium bromide and oxacillin against <i>Staphylococcus epidermidis</i>.","authors":"Lehong Yuan, Pengfei She","doi":"10.11817/j.issn.1672-7347.2024.240109","DOIUrl":"10.11817/j.issn.1672-7347.2024.240109","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;&lt;i&gt;Staphylococcus epidermidis&lt;/i&gt; (&lt;i&gt;S. epidermidis&lt;/i&gt;) adheres to the surface of medical devices, forming highly drug-resistant biofilms, which has made the development of novel antibacterial agents against &lt;i&gt;S. epidermidis&lt;/i&gt; and its biofilms a key research focus. By drug repurposing, this study aims to explore the combinational antimicrobial effects between pinaverium bromide (PVB), a &lt;i&gt;L&lt;/i&gt;-type calcium channel blocker, and oxacillin (OXA) against &lt;i&gt;S. epidermidis&lt;/i&gt;.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Clinical isolates of &lt;i&gt;S. epidermidis&lt;/i&gt; were collected from January to September 2022 at the Department of Clinical Laboratory of the Third Xiangya Hospital, Central South University. The minimal inhibitory concentrations (MICs) of PVB and OXA were determined using the broth microdilution method. Checkerboard assays and time-kill curves were performed to assess the fractional inhibitory concentration index and synergistic bactericidal efficiency of the drug combination. Resistance selection assays evaluated PVB's ability to inhibit the development of OXA resistance. Biofilm eradication assays, combined with confocal laser scanning microscopy (CLSM) and the persister cell quantification, were conducted to evaluate the effect of PVB and OXA on drug-resistant biofilms and persister cells. The mechanisms of PVB action were further investigated using transmission electronic microscopy (TEM), reactive oxygen species (ROS) quantification, and ATP quantification.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The MICs of PVB and OXA against the standard strain &lt;i&gt;S. epidermidis&lt;/i&gt; RP62A were both 8 μg/mL. Checkerboard assays showed that the fractional inhibitory concentration index (FICI) for the combination was 0.250 0 for RP62A and ranged from 0.187 5 to 0.500 0 for clinical isolates, indicating synergistic effects. Resistance selection assays demonstrated that PVB not only failed to induce resistance but also effectively inhibited the development of OXA resistance. The combination of 1×MIC of PVB and OXA reduced biofilm biomass (A&lt;sub&gt;570 nm&lt;/sub&gt;) from (2.36±0.46) to (1.12±0.39) (&lt;i&gt;t&lt;/i&gt;=3.504, &lt;i&gt;P&lt;/i&gt;=0.02). CLSM revealed significant biofilm structural disruption and an increased proportion of dead bacteria. Additionally, after 4 hours of treatment, the total persister cell count was reduced from lg(7.73±0.21) to lg(2.79±0.43) (&lt;i&gt;t&lt;/i&gt;=4.143, &lt;i&gt;P&lt;/i&gt;=0.014). Synergistic biofilm eradication was further confirmed in clinical isolates. TEM revealed that PVB caused significant bacterial structural damage. The combination of OXA and PVB significantly induced ROS production, increasing the relative fluorescence intensity from (30 000.00±2 000.00) to (45 666.67±2 081.67) (&lt;i&gt;t&lt;/i&gt;=10.68, &lt;i&gt;P&lt;/i&gt;&lt;0.001), and markedly reduced ATP generation, lowering the relative fluorescence intensity form (565.00±33.18) to (205.67±35.23) (&lt;i&gt;t&lt;/i&gt;=4.932, &lt;i&gt;P&lt;/i&gt;=0.003).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;The combination of PVB and OXA exh","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 10","pages":"1601-1610"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11897976/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}