中南大学学报(医学版)最新文献

{"title":"Reprogramming astrocytes into dopaminergic neurons to restore motor dysfunction in Parkinson<b>'</b>s disease model rats.","authors":"Chunbo Liu, Mengjiao Ying, Ao Wang, Yumeng Liu, Ying Chen, Wenhao Ye, Hebao Wen, Caiyun Ma, Changqing Liu, Yu Guo","doi":"10.11817/j.issn.1672-7347.2024.240078","DOIUrl":"10.11817/j.issn.1672-7347.2024.240078","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;Parkinson's disease (PD) is a neurodegenerative disorder primarily caused by the loss of dopaminergic neurons (DA) in the brain. Since DA neurons are non-renewable, conventional therapies only alleviate symptoms without addressing the root cause. This study aims to reprogram astrocyte (AS) into DA neurons for transplantation into the brain to reconstruct damaged neural circuits and treat PD.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Astrocytes were isolated from neonatal rat brain tissues. A lentiviral vector carrying the transcription factors nuclear receptor-related factor 1 (UNRR1) and achaete-scute family bHLH transcription factor 1 (ASCL1), named LV-NURR1-ASCL1, was constructed and used to infect cultured rat AS in vitro. Immunofluorescence, Western blotting, and reverse transcription polymerase chain reaction (RT-PCR) were employed to detect and compare the expression levels of &lt;i&gt;NURR1&lt;/i&gt; and &lt;i&gt;ASCL1&lt;/i&gt; in lentivirus-infected AS (LV group) and AS cultured in complete medium without LV-NURR1-ASCL1 (Con group). The virus-infected AS was then cultured in neuronal induction medium for 18 days. Immunofluorescence was used to detect the expression of DA markers, including tyrosine hydroxylase (TH) and forkhead box A2 (FOXA2), as well as the neuronal marker class III beta tubulin (TUJ1). To establish the PD rat model, 6-hydroxydopamine (6-OHDA) was injected into 2 sites in the medial forebrain bundle (MFB) region of the right brain in rats. The reprogrammed cells (AS-iDA) were quantified and transplanted into the right MFB region of the PD model rats using a stereotaxic instrument. Four weeks after transplantation, immunofluorescence was used to assess the survival, differentiation, and migration of AS-iDA in the brain and the expression of TH, TUJ1, and FOXA2 in the brain tissue of PD rats. Eight weeks post-transplantation, the recovery of motor function in PD rats was evaluated using the apomorphine (APO)-induced rotation test, rotarod fatigue test, and open-field test.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Immunofluorescence analysis showed positive expression of NURR1 and ASCL1 in AS after lentiviral infection. RT-PCR results demonstrated that the mRNA expression levels of &lt;i&gt;NURR1&lt;/i&gt; and &lt;i&gt;ASCL1&lt;/i&gt; in the LV group were significantly higher than those in the Con group, with increases of (7.483±0.706)-fold and (10.830±1.940)-fold, respectively. Western blotting analysis further confirmed that the protein expression levels of NURR1 and ASCL1 in the LV group were (2.403±0.511)-fold and (4.423±0.603)-fold higher, respectively, compared to the control group. After 18 days of directed induction culture lentivirus-infected AS (AS-iDA) displayed significant morphological changes, developing neuron-like long neurites. At this stage, AS-iDA highly expressed the neuronal marker TUJ1 as well as the DA markers TH and FOXA2. Four weeks post-transplantation, immunofluorescence on brain slices from PD rats revealed that AS-iDA survived ","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 9","pages":"1377-1389"},"PeriodicalIF":0.0,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anesthetic management of a patient with closed tracheal transection.","authors":"Songkai Long, Gaijun Huang, Yuanyuan Guo, Zhujun Huang, Hu Yi","doi":"10.11817/j.issn.1672-7347.2024.240265","DOIUrl":"10.11817/j.issn.1672-7347.2024.240265","url":null,"abstract":"<p><p>Closed tracheal injury is a rare clinical emergency that is prone to misdiagnosis and missed diagnosis. Improper management can be life-threatening. This report describes the anesthetic management of a patient with closed tracheal transection undergoing airway reconstruction surgery. The patient, a 33-year-old male, presented with head and neck pain and dyspnea 4 hours after a car accident. CT with 3-dimensional airway reconstruction revealed upper tracheal transection. Emergency cervical exploration and end-to-end tracheal anastomosis were performed under general anesthesia. The anesthetic approach included preserving spontaneous respiration with mild sedation and analgesia, followed by fiberoptic bronchoscope-guided nasal tracheal intubation after confirming the transection site. The airway was successfully reconstructed in cooperation with the surgical team. The patient recovered well postoperatively and was discharged without complications. One-year follow-up showed no adverse outcomes. For patients with closed tracheal trauma, anesthesiologists must remain vigilant develop a comprehensive anesthetic plan, ensure effective ventilation, and avoid blind tracheal intubation.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 9","pages":"1538-1542"},"PeriodicalIF":0.0,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of subretinal injection of tissue-type plasminogen activator for the treatment of submacular hemorrhage complicating polypoidal choroidal vasculopathy.","authors":"Guoli Zheng, Nan Wang, Wenbo Lei, Yao Xu, Xiaobo Xia, Siqi Xiong","doi":"10.11817/j.issn.1672-7347.2024.230488","DOIUrl":"10.11817/j.issn.1672-7347.2024.230488","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;Polypoidal choroidal vasculopathy (PCV) is a major cause of vision loss among older adults worldwide and is particularly common in Asians. When the aneurysmal dilatation of blood vessels or the rupture of widened small veins/arteries occurs, it leads to submacular hemorrhage (SMH), which can result in irreversible damage to vision over time. This study aims to evaluate the surgical outcomes and complications of pars plana vitrectomy (PPV) combined with intraoperative subretinal injection of tissue-type plasminogen activator (t-PA) and postoperative intravitreal injection of anti-vascular endothelial growth factor (VEGF) for the treatment of SMH secondary to PCV.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Thirteen patients (13 eyes) with SMH secondary to PCV treated at the Eye Center, Xiangya Hospital, Central South University, from September 2020 to December 2021, were included in this study. All patients underwent PPV combined with subretinal injection of t-PA during surgery and received postoperative intravitreal injections of anti-VEGF. Best corrected visual acuity (BCVA), intraocular pressure, absorption of subretinal hemorrhage, progression of the primary lesion, surgical complications, and factors associated with postoperative efficacy were analyzed preoperatively, 1 week postoperatively, 1 month postoperatively, and 6 months postoperatively.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;All patients completed the 6-month follow-up, and at the final visit, 100% of the subretinal hemorrhage of 13 patients was completely cleared. BCVA significantly improved at both 1 month and 6 months postoperatively compared to preoperative and 1-week postoperative levels (all &lt;i&gt;P&lt;/i&gt;&lt;0.05). Patients over 60 years old and those with lesions located beneath the fovea had statistically significant differences in visual recovery compared to younger patients and those with extrafoveal lesions (&lt;i&gt;P&lt;/i&gt;=0.045 and &lt;i&gt;P&lt;/i&gt;=0.006, respectively). No significant correlation was found between disease duration, extent of hemorrhage, presence or absence of preoperative vitreous hemorrhage (VH), and visual recovery at 6 months postoperatively (all &lt;i&gt;P&lt;/i&gt;&gt;0.05). Ten patients had an intact ellipsoid zone, and 5 patients had an intact retinal pigment epithelium layer, however, the difference in preoperative and postoperative visual acuity between those with and without intact microstructures was not statistically significant (all &lt;i&gt;P&lt;/i&gt;&gt;0.05). At the final follow-up, 2 patients had retinal neuroepithelial edema, and 1 patient had retinal pigment epithelium layer detachment. Postoperative complications included 1 patient of macular hole and 1 patient of retinal pigment epithelium layer tear.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;PPV combined with subretinal t-PA injection and postoperative intravitreal anti-VEGF injection effectively clears SMH secondary to PVC with few surgical complications and significantly improves visual function of the patients over 6-month ","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 9","pages":"1431-1439"},"PeriodicalIF":0.0,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends in disease burden of ADHD in China from 1990 to 2019 based on GBD analysis.","authors":"Nan Zheng, Jun Tang, Kexin Duan, Juntao Zhao, Xiaomei Ren","doi":"10.11817/j.issn.1672-7347.2024.240010","DOIUrl":"10.11817/j.issn.1672-7347.2024.240010","url":null,"abstract":"<p><strong>Objectives: </strong>Attention deficit hyperactivity disorder (ADHD) has a significant impact on individual health and society. This study aims to analyze the disease burden of ADHD in China from 1990 to 2019 and make predictions to provide a basis for the formulation of corresponding prevention and control strategies by the government.</p><p><strong>Methods: </strong>Using data from Global Burden of Diseases (GBD) 2019 database, we described the changes in incidence, prevalence, and disability-adjusted life years (DALY) in China from 1990 to 2019. An autoregressive integrated moving average model (ARIMA) was used to predict the disease burden from 2020 to 2029.</p><p><strong>Results: </strong>From 1990 to 2019, the incidence, prevalence and DALY rates of ADHD all showed an upward trend, with standardized rates for males significantly higher than those for females. The age group of 5 to 9 years showed a higher incidence. Both prevalence and DALY rates peaked at ages 10 to 14, gradually declining with increasing age, although ADHD continues to impose a certain disease burden on adults. Prediction results indicate a slow decline in the standardized incidence rate of ADHD in China from 2020 to 2029, with standardized prevalence and standardized DALY rates showing a gradual increase from 2020 to 2025, followed by a decrease, potentially reaching 112.28/100 000, 2 070.03/100 000, and 25.41/100 000 by 2 029, respectively.</p><p><strong>Conclusions: </strong>ADHD is a condition that spans the entire lifespan, yet current recognition, diagnosis, and treatment of adult ADHD remain insufficient. It is recommended to focus on the early diagnosis and treatment of adult ADHD based on existing disease monitoring to mitigate the impact of the disease on patients and society.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 9","pages":"1449-1455"},"PeriodicalIF":0.0,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Hippo-YAP/TAZ signaling pathway in organ fibrosis.","authors":"Junqing Wang, Linsheng Huang, Huifan Yu, Jingyu Liu, Lu Yuan, Fei Li","doi":"10.11817/j.issn.1672-7347.2024.230577","DOIUrl":"10.11817/j.issn.1672-7347.2024.230577","url":null,"abstract":"<p><p>Organ fibrosis is closely associated with inflammation, tissue injury, and abnormal repair processes, characterized primarily by the activation of myofibroblasts and excessive extracellular matrix deposition. While the clinical manifestations and pathogenesis of fibrosis vary across organs, the progression of fibrosis can disrupt normal organ function, leading to disability or even death. The Hippo signaling pathway, an evolutionarily conserved kinase cascade, plays a pivotal role in regulating cell proliferation, apoptosis, differentiation, and tissue regeneration. Its primary effectors, yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ), are critically implicated in fibrosis of organs such as the kidney, liver, lung, heart, and skin. Currently, effective and specific treatments for organ fibrosis are lacking. A comprehensive understanding of the relationship between Hippo-YAP/TAZ signaling and organ fibrosis may provide novel perspectives for the clinical management of these conditions.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 9","pages":"1509-1516"},"PeriodicalIF":0.0,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Value of serum tumor markers in early differential diagnosis of spinal tumors and spinal infections.","authors":"Bo Tang, Xiaojiang Hu, Dongcheng Xu, Guang Zhang, Hongqi Zhang, Chaofeng Guo, Shaohua Liu, Qile Gao, Yanbing Li, Mingxing Tang","doi":"10.11817/j.issn.1672-7347.2024.230603","DOIUrl":"10.11817/j.issn.1672-7347.2024.230603","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;The early imaging features of spinal tumors and spinal infections are similar, and the lack of specific early diagnostic indicators can lead to misdiagnosis or missed diagnosis. Although serum tumor markers have been widely used in early cancer screening, spinal tumors are mostly metastatic, lacking specific markers, and some patients with spinal tumors may even test negative for tumor markers. Therefore, the role of tumor markers in the early differential diagnosis of spinal tumors remains unclear. This study aims to investigate the value of tumor markers in the early differential diagnosis of spinal tumors and spinal infection.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;We retrospectively analyzed the clinical data of 221 patients with spinal bone destruction admitted to Xiangya Hospital of Central South University between April 2017 and October 2022. Peripheral blood levels of 10 tumor markers were measured upon admission, including carcinoembryonic antigen (CEA), alpha fetoprotein (AFP), neuron-specific enolase (NSE), cytokeratin 19 fragment antigen 21-1 (cyfra21-1), carbohydrate antigen 199 (CA199), carbohydrate antigen 125 (CA125), carbohydrate antigen 72-4 (CA72-4), pepsinogen I (PGI), pepsinogen II (PGII), and the PGI/PGII ratio (PGR). Univariate Logistic analysis was used to screen relevant variables, and the correlation between tumor markers was analyzed. Multivariate Logistic regression was then employed to identify risk and protective factors. The optimal cut-off values were calculated using the receiver operating characteristic (ROC) curve and Youden index, and a differential diagnosis model for early spinal tumors and spinal infections was constructed based on the selected indicators. Diagnostic performance was then evaluated.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;According to the pathological diagnosis, 91 patients had spinal tumors, and 130 patients had spinal infections. The levels of CEA, AFP, NSE, cyfra21-1, CA199, and CA72-4 were higher in the spinal tumor group than those in the spinal infection group, while PGR was lower, with statistically significant differences (all &lt;i&gt;P&lt;/i&gt;&lt;0.05). Univariate Logistic analysis showed significant differences in CEA, AFP, NSE, cyfra21-1, CA199, CA125, CA72-4, and PGR (all &lt;i&gt;P&lt;/i&gt;&lt;0.05). Correlation analysis indicated a strong positive correlation between CEA and cyfra21-1, CA199, and CA125, as well as a strong negative correlation of PGII with PGI and PGR. Multivariate Logistic analysis identified AFP and cyfra21-1 as risk factors (&lt;i&gt;P&lt;/i&gt;&lt;0.01) and PGR as a protective factor (&lt;i&gt;P&lt;/i&gt;&lt;0.05). When the standard cut-off values (AFP&lt;20 ng/mL, cyfra21-1&lt; 3.3 ng/mL, and PGR&gt;3) were applied, AFP had a sensitivity of 3.3% and accuracy of 60.2%, cyfra21-1 had a sensitivity of 20.9% and accuracy of 66.1%, and PGR had a sensitivity of 2.2% and accuracy of 59.3%. The AUC of the early spinal tumor diagnosis model was 0.623. Using the optimal cut-off values (AFP&lt;1.625 ng/mL, cyfra21-1&lt;1","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 9","pages":"1421-1430"},"PeriodicalIF":0.0,"publicationDate":"2024-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research progress on the relationship between Nesfatin-1 and glucose metabolism.","authors":"Yunting Cao, Wei Wang","doi":"10.11817/j.issn.1672-7347.2024.240113","DOIUrl":"10.11817/j.issn.1672-7347.2024.240113","url":null,"abstract":"<p><p>Nesfatin-1 is a neuropeptide hormone known for its biological functions, including inhibiting food intake, regulating glucose and lipid metabolism, promoting apoptosis, and providing anti-inflammatory and anti-tumor effects. Glucose metabolism is a crucial pathway for the body's energy supply. Current research has demonstrated that Nesfatin-1 can affect glucose metabolism through various mechanisms, such as inhibiting food intake, regulating enzyme activity, and improving insulin resistance, though the findings are not entirely consistent. Investigating the relationship between Nesfatin-1 and glucose metabolism may offer new insights into the diagnosis and treatment of diseases related to glucose metabolism disorders.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 6","pages":"832-838"},"PeriodicalIF":0.0,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11420965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142297755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maresin 1 alleviates neuroinflammation and cognitive decline in a mouse model of cecal ligation and puncture.","authors":"Longyan Li, Manyu Xing, Lu Wang, Yixia Zhao","doi":"10.11817/j.issn.1672-7347.2024.240117","DOIUrl":"10.11817/j.issn.1672-7347.2024.240117","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;Inflammation in the central nervous system plays a crucial role in the occurrence and development of sepsis-associated encephalopathy. This study aims to explore the effects of maresin 1 (MaR1), an anti-inflammatory and pro-resolving lipid mediator, on sepsis-induced neuroinflammation and cognitive impairment.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Mice were randomly assigned to 4 groups: A sham group (sham operation+vehicle), a cecal ligation and puncture (CLP) group (CLP operation+vehicle), a MaR1-LD group (CLP operation+1 ng MaR1), and a MaR1-HD group (CLP operation+10 ng MaR1). MaR1 or vehicle was intraperitoneally administered starting 1 h before CLP operation, then every other day for 7 days. Survival rates were monitored, and serum inflammatory cytokines [tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, and IL-6] were measured 24 h after operation using enzyme-linked immunosorbent assay (ELISA). Cognitive function was assessed 7 days after operation using the Morris water maze (MWM) test and novel object recognition (NOR) task. The mRNA expression of &lt;i&gt;TNF-α&lt;/i&gt;, &lt;i&gt;IL-1β&lt;/i&gt;, &lt;i&gt;IL-6&lt;/i&gt;, inducible nitric oxide synthase (&lt;i&gt;iNOS&lt;/i&gt;), &lt;i&gt;IL-4&lt;/i&gt;, &lt;i&gt;IL-10&lt;/i&gt;, and arginase 1 (&lt;i&gt;Arg1&lt;/i&gt;) in cortical and hippocampal tissues was determined by real-time reverse transcription PCR (RT-PCR). Western blotting was used to determine the protein expression of iNOS, Arg1, signal transducer and activator of transcription 6 (STAT6), peroxisome proliferator-activated receptor gamma (PPARγ), and phosphorylated STAT6 (p-STAT6) in hippocampal tissue. Microglia activation was visualized via immunofluorescence. Mice were also treated with the PPARγ antagonist GW9662 to confirm the involvement of this pathway in MaR1's effects.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;CLP increased serum levels of TNF-α, IL-1β, and IL-6, and reduced body weight and survival rates (all &lt;i&gt;P&lt;/i&gt;&lt;0.05). Both 1 ng and 10 ng doses of MaR1 significantly reduced serum TNF-α, IL-1β, and IL-6 levels, improved body weight, and increased survival rates (all &lt;i&gt;P&lt;/i&gt;&lt;0.05). No significant difference in efficacy was observed between the 2 doses (all &lt;i&gt;P&lt;/i&gt;&gt;0.05). MWM test and NOR task indicated that CLP impaired spatial learning, which MaR1 mitigated. However, GW9662 partially reversed MaR1's protective effects. Real-time RT-PCR results demonstrated that, compared to the sham group, mRNA expression of &lt;i&gt;TNF-α&lt;/i&gt;, &lt;i&gt;IL-1β,&lt;/i&gt; and &lt;i&gt;iNOS&lt;/i&gt; significantly increased in hippocampal tissues following CLP (all &lt;i&gt;P&lt;/i&gt;&lt;0.05), while &lt;i&gt;IL-4&lt;/i&gt;, &lt;i&gt;IL-10&lt;/i&gt;, and &lt;i&gt;Arg1&lt;/i&gt; showed a slight decrease, though the differences were not statistically significant (all &lt;i&gt;P&lt;/i&gt;&gt;0.05). Compared to the CLP group, both 1 ng and 10 ng MaR1 decreased &lt;i&gt;TNF-α&lt;/i&gt;, &lt;i&gt;IL-1β&lt;/i&gt;, and &lt;i&gt;iNOS&lt;/i&gt; mRNA expression in hippocampal tissues and increased &lt;i&gt;IL-4&lt;/i&gt;, &lt;i&gt;IL-10&lt;/i&gt;, and &lt;i&gt;Arg1&lt;/i&gt; mRNA expression (all &lt;i&gt;P&lt;/i&gt;&lt;0.05). Immunofluorescence results indicated a significa","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 6","pages":"890-902"},"PeriodicalIF":0.0,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11420972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142297751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mediating role of inner strength in the relationship between medication literacy and medication adherence among kidney transplant patients.","authors":"Liping Wang, Chunhua Fang, Manhua Nie, Li Zhu, Sai Liu, Haiyang Li","doi":"10.11817/j.issn.1672-7347.2024.240215","DOIUrl":"10.11817/j.issn.1672-7347.2024.240215","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objectives: &lt;/strong&gt;Compared with long-term renal replacement therapy, kidney transplantation is the ideal treatment for end-stage renal disease (ESRD), significantly extending patient life and improving quality of life. Kidney transplant patients need to adhere to lifelong immunosuppressive medication regimens, but their medication adherence is generally poor compared with other organ transplant recipients. Medication adherence is closely related to medication literacy and psychological status, yet related studies are limited. This study aims to investigate the current status of medication adherence, inner strength, and medication literacy in kidney transplant patients, analyze the relationships among these 3 factors, and explore the mediating role of inner strength in the relationship between medication literacy and medication adherence.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A cross-sectional survey was conducted from March to October 2023 involving 421 patients aged≥18 years who visited kidney transplantation outpatient clinics at 4 tertiary hospitals in Hunan Province. The inner strength, medication literacy, and medication adherence of kidney transplant patients were investigated using the Inner Strength Scale (ISS), the Chinese version of the Medication Literacy Assessment in Spanish and English (MedLitRxSE), and the Chinese version of the Morisky Medication Adherence Scale-8 (C-MMAS-8), respectively. Univariate analysis was performed to examine the effects of demographic and clinical data on medication adherence. Correlation analysis was conducted to explore the relationships among medication literacy, medication adherence, and inner strength. Significant variables from univariate and correlation analyses were further analyzed using multiple linear regression, and the mediating effect of inner strength was explored.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Among the 421 questionnaires collected, 408 were valid, with an effective rate of 96.91%. The scores of C-MMAS-8, MedLitRxSE, and ISS were 6.64±1.16, 100.63±14.67, and 8.47±4.03, respectively. Among the 408 patients, only 86 (21.08%) patients had a high level of medication adherence, whereas 230 (56.37%) patients had a medium level of medication adherence, and 92 (22.55%) patients had poor medication adherence. Univariate analysis indicated that the kidney transplant patients' age, marital status, education levels, years since their kidney transplant operation, number of hospitalizations after the kidney transplant, and adverse drug reactions showed significant differences in medication adherence (all &lt;i&gt;P&lt;/i&gt;&lt;0.05). Correlation analysis showed that inner strength positively correlated with both medication literacy (&lt;i&gt;r&lt;/i&gt;=0.183, &lt;i&gt;P&lt;/i&gt;&lt;0.001) and medication adherence (&lt;i&gt;r&lt;/i&gt;=0.201, &lt;i&gt;P&lt;/i&gt;&lt;0.001). Additionally, there was a positive correlation between medication adherence and medication literacy (&lt;i&gt;r&lt;/i&gt;=0.236, &lt;i&gt;P&lt;/i&gt;&lt;0.001). Inner strength accounted for 13.22% of the total effec","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 6","pages":"961-971"},"PeriodicalIF":0.0,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11420961/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142297753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-sample Mendelian randomization analysis of causal relationship between eczema and autoimmune diseases.","authors":"Chunli Chen, Siyu Yan, Bangbei Wan, Yangyiyi Yu, Jinrong Zeng, Lina Tan, Jianyun Lu","doi":"10.11817/j.issn.1672-7347.2024.240103","DOIUrl":"10.11817/j.issn.1672-7347.2024.240103","url":null,"abstract":"<p><strong>Objectives: </strong>The causal relationship between eczema and autoimmune diseases has not been previously reported. This study aims to evaluate the causal relationship between eczema and autoimmune diseases.</p><p><strong>Methods: </strong>The two-sample Mendelian randomization (MR) method was used to assess the causal effect of eczema on autoimmune diseases. Summary data from the Genome-Wide Association Study Catalog (GWAS) were obtained from the Integrative Epidemiology Unit (IEU) database. For eczema and autoimmune diseases, genetic instrument variants (GIVs) were identified according to the significant difference (<i>P</i><5×10^-8). Causal effect estimates were generated using the inverse-variance weighted (IVW) method. MR Egger, maximum likelihood, MR-PRESSO, and MR-RAPS methods were used for alternative analyses. Sensitivity tests, including heterogeneity, horizontal pleiotropy, and leave-one-out analyses, were performed. Finally, reverse causality was assessed.</p><p><strong>Results: </strong>Genetic susceptibility to eczema was associated with an increased risk of Crohn's disease (<i>OR</i>=1.444, 95% <i>CI</i> 1.199 to 1.738, <i>P</i><0.001) and ulcerative colitis (<i>OR</i>=1.002, 95% <i>CI</i> 1.001 to 1.003, <i>P</i>=0.002). However, no causal relationship was found for the other 6 autoimmune diseases, including systemic lupus erythematosus (SLE) (<i>OR</i>=0.932, <i>P</i>=0.401), bullous pemphigoid (BP) (<i>OR</i>=1.191, <i>P</i>=0.642), vitiligo (<i>OR</i>=1.000, <i>P</i>=0.327), multiple sclerosis (MS) (<i>OR</i>=1.000, <i>P</i>=0.965), ankylosing spondylitis (AS) (<i>OR</i>=1.001, <i>P</i>=0.121), rheumatoid arthritis (RA) (<i>OR</i>=1.000, <i>P</i>=0.460). Additionally, no reverse causal relationship was found between autoimmune diseases and eczema.</p><p><strong>Conclusions: </strong>Eczema is associated with an increased risk of Crohn's disease and ulcerative colitis. No causal relationship is found between eczema and SLE, MS, AS, RA, BP, or vitiligo.</p>","PeriodicalId":39801,"journal":{"name":"中南大学学报(医学版)","volume":"49 6","pages":"932-942"},"PeriodicalIF":0.0,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11420964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142297788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}