Identification of apoptosis-related long non-coding RNAs expression profiles in patient with cervical intraepithelial neoplasia 3.

Q3 Medicine

中南大学学报(医学版) Pub Date : 2024-07-28 DOI:10.11817/j.issn.1672-7347.2024.230302

Zhaoyang Liu, Lingli Luo, Xinyi Wu, Bingqi Wang, Min Wang, Xianping Li

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引用次数: 0

Abstract

Objectives: Long non-coding RNAs (lncRNAs) play an essential role in cancer biology. Cervical intraepithelial neoplasia grade 3 (CIN3) is the most severe precancerous lesion of cervical cancer. However, the mechanism of multiple lncRNAs in CIN3 has not been studied in-depth and is worth exploring. This study aims to summarize the lncRNA expression profile in CIN 3 and screen for lncRNAs with potential oncogenic effects.

Methods: To further clarify the role of lncRNAs in the development of CIN3, this study collected cancer tissue and para-cancer tissue specimens from three CIN3 patients. The RNA sequencing (RNA-Seq) analysis was used to construct expression profiles of lncRNAs and confirmed by real-time reverse transcription PCR (real-time RT-PCR) methods. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were performed with computational methods. We obtained their possible target genes through the cis- and trans-regulatory analysis of the lncRNAs. In addition, this study predicted new transcripts and lncRNAs by Cufflinks analysis and coding potential identification (Coding Potential Calculator) analysis to discover new tumor regulatory molecules.

Results: The differential expressions of 1 555 genes, 1 562 mRNAs, 241 ncRNAs, and 6 616 new predicted-lncRNAs were found. The most prominently up-regulated and down-regulated lncRNAs were NR_145433.1 and ENST00000513672.1, respectively. KEGG analysis revealed that lncRNA-targeted genes were closely related to apoptosis, metabolic, p53 signaling pathway, and other cancer-related pathways. The real-time RT-PCR validation results of 5 apoptosis-related lncRNAs (NR_145433.1, ENST00000510610.2, UC001Kfo, ENST00000602964.1, and NR_123733.1) in cervical cancer were consistent with the sequencing results.

Conclusions: This study provides a comprehensive expression profile of lncRNAs in CIN3 patients, particularly the apoptosis-related expression profile of lncRNAs, and provides direction and clues for the study of cervical cancer and the search for potential therapeutic targets.

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宫颈上皮内瘤变患者细胞凋亡相关长链非编码rna表达谱的鉴定

目的：长链非编码rna （lncRNAs）在肿瘤生物学中发挥着重要作用。宫颈上皮内瘤变3级（CIN3）是宫颈癌中最严重的癌前病变。然而，多种lncrna在CIN3中的作用机制尚未得到深入研究，值得探讨。本研究旨在总结lncRNA在CIN 3中的表达谱，筛选具有潜在致癌作用的lncRNA。方法：为了进一步明确lncrna在CIN3发生发展中的作用，本研究收集了3例CIN3患者的癌组织和癌旁组织标本。采用RNA测序（RNA- seq）分析构建lncRNAs的表达谱，并采用实时反转录PCR （real-time RT-PCR）方法进行验证。采用计算方法对基因本体（GO）和京都基因与基因组百科全书（KEGG）进行计算。我们通过对lncrna的顺式和反式调控分析获得了它们可能的靶基因。此外，本研究通过袖扣分析和编码电位鉴定（coding potential Calculator）分析预测新的转录本和lncrna，发现新的肿瘤调控分子。结果：共发现差异表达基因1555个，mrna 1562个，ncrna 241个，预测lncrna 6 616个。上调和下调最显著的lncrna分别是NR_145433.1和ENST00000513672.1。KEGG分析显示lncrna靶向基因与细胞凋亡、代谢、p53信号通路等肿瘤相关通路密切相关。5个凋亡相关lncrna （NR_145433.1、ENST00000510610.2、UC001Kfo、ENST00000602964.1、NR_123733.1）在宫颈癌中的实时RT-PCR验证结果与测序结果一致。结论：本研究提供了完整的lncRNAs在CIN3患者中的表达谱，特别是lncRNAs与凋亡相关的表达谱，为宫颈癌的研究和寻找潜在的治疗靶点提供了方向和线索。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

中南大学学报(医学版) Medicine-Medicine (all)

CiteScore

1.00

自引率

0.00%

发文量

8237

期刊介绍： Journal of Central South University (Medical Sciences), founded in 1958, is a comprehensive academic journal of medicine and health sponsored by the Ministry of Education and Central South University. The journal has been included in many important databases and authoritative abstract journals at home and abroad, such as the American Medline, Pubmed and its Index Medicus (IM), the Netherlands Medical Abstracts (EM), the American Chemical Abstracts (CA), the WHO Western Pacific Region Medical Index (WPRIM), and the Chinese Science Citation Database (Core Database) (CSCD); it is a statistical source journal of Chinese scientific and technological papers, a Chinese core journal, and a "double-effect" journal of the Chinese Journal Matrix; it is the "2nd, 3rd, and 4th China University Excellent Science and Technology Journal", "2008 China Excellent Science and Technology Journal", "RCCSE China Authoritative Academic Journal (A+)" and Hunan Province's "Top Ten Science and Technology Journals". The purpose of the journal is to reflect the new achievements, new technologies, and new experiences in medical research, medical treatment, and teaching, report new medical trends at home and abroad, promote academic exchanges, improve academic standards, and promote scientific and technological progress.