The Egyptian journal of immunology / Egyptian Association of Immunologists最新文献_第2页

Role of vitamin D receptor genetic variants (ApaI and FokI) in association with nephropathy stages in a group of Egyptian patients with type 2 diabetes mellitus. 维生素D受体遗传变异（ApaI和FokI）在一组埃及2型糖尿病患者肾病分期中的作用

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2025-04-01 DOI: 10.55133/eji.320206

Asmaa M Fteah, Samah Mamdouh, Samia El-Shishtawy, Nevine Sherif, Doaa M Aly

{"title":"Role of vitamin D receptor genetic variants (ApaI and FokI) in association with nephropathy stages in a group of Egyptian patients with type 2 diabetes mellitus.","authors":"Asmaa M Fteah, Samah Mamdouh, Samia El-Shishtawy, Nevine Sherif, Doaa M Aly","doi":"10.55133/eji.320206","DOIUrl":"https://doi.org/10.55133/eji.320206","url":null,"abstract":"Diabetic nephropathy (DN) is one of the most worrisome complications of diabetes, causing significant social and economic impacts. Genetic polymorphisms in vitamin D receptor (VDR) gene may lead to genomic instability and increase susceptibility to end-stage renal disease (ESRD). In this research, we aimed to identify the association of genetic variants: ApaI \"rs7975232\" and FokI \"rs10735810\" in the VDR gene with nephropathy stages in diabetic patients. This case-control hospital-based study included 200 Egyptian participants divided into a group of 150 patients with type 2 diabetes mellitus (T2DM), divided into three subgroups according to albumin/creatinine ratio, and 50 age and sex matched participants as a normal control group. Genetic variants in the VDR gene were detected using restriction fragment length polymerase chain reaction to evaluate their association with kidney disease stage and bone density in T2DM patients. Our results revealed that aa genotype and a allele frequency in ApaI \"rs7975232\" and ff genotype and f allele frequency in FokI \"rs10735810\" were more frequent in diabetic patients than in the normal control group (p < 0.001). In addition, our results revealed that T2DM patients with the ApaI aa genotype and a allele were at a higher risk of developing ESRD as they were almost 13-fold higher than those with the (Aa/AA) genotype and A allele. Also, we found that carriers of the ff genotype and f allele of FokI are at 17-fold and 7-fold higher risk of ESRD than carriers of the non-ff genotype. In conclusion, our study findings indicated that the FokI f allele and the ApaI a allele variant of VDR gene could be used as molecular biomarkers to predict the risk of diabetes and nephropathy stages in Egyptian patients.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 2","pages":"57-69"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soluble CD8 and CD25 along with anti-tTG autoantibody as non-invasive prognostic factor in celiac patients. 可溶性CD8和CD25与抗ttg自身抗体作为乳糜泻患者的非侵入性预后因素

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2025-04-01 DOI: 10.55133/eji.320202

Karrar A Kamil, Mayyada F Darweesh

{"title":"Soluble CD8 and CD25 along with anti-tTG autoantibody as non-invasive prognostic factor in celiac patients.","authors":"Karrar A Kamil, Mayyada F Darweesh","doi":"10.55133/eji.320202","DOIUrl":"https://doi.org/10.55133/eji.320202","url":null,"abstract":"Celiac disease (CeD) is an enteropathy autoimmune disease that affects about 1% of people worldwide with various implications including health, psychological well-being, and economic effect for individuals and families. Elevated systemic levels of soluble cluster of differentiation 4 (sCD4) associated with different diseases, but the association of sCD8 and sCD25 levels in celiac disease was not investigated. This case-control study was designed to investigate the role of sCD8 and sCD25 as prognostic factors in celiac disease. The study included 120 samples from patients with CeD and normal people during the period from September 2023 to January 2024. The samples included both genders and different ages. Blood samples (5 ml) were drawn from each study subject. Soluble CD8, Soluble CD25, anti-glutamic acid decarboxylase (anti-GAD), and anti-tissue transglutaminase antibody (anti-tTG) were measured by the ELISA technique. There was no significant difference in age and gender between CeD patients and controls. The mean serum level of anti-tTG antibody was significantly higher in CeD patients (87.15 ±10.34) than controls (57.35±7.32 U/ml). for IgA and IgG than the control group 2.51±0.35 and 3.39±0.36 U/ml for IgA and IgG, respectively. Also, the serum level of anti-GAD was increased in patients compared to the control group. CeD patients had higher mean level of soluble CD8 and CD25 (10.77±0.778 and 1356.15±83.31 pmol/l) compared to the control group (3.98±0.29and 487.23±96.22 pmol/l). The study concluded that anti-TtG and CD25 can be used as non-invasive serum biomarkers to predict disease progression. By monitoring the levels of soluble CD8 and CD25 with anti-tTG-IgA in their serum, healthcare providers can predict clinical outcomes and identify the inflammatory process associated with CeD.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 2","pages":"17-26"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144052676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

UBASH3A and TIGIT genes expression levels in systemic sclerosis. UBASH3A和TIGIT基因在系统性硬化症中的表达水平。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2025-04-01 DOI: 10.55133/eji.320209

Aml A R Mohammed, Fatma S Abd-Elsamea, Maha S I Abdelrahman, Fatma M A Mohamed, Fatma Y A Abbas, Fatma M Helbawi

{"title":"UBASH3A and TIGIT genes expression levels in systemic sclerosis.","authors":"Aml A R Mohammed, Fatma S Abd-Elsamea, Maha S I Abdelrahman, Fatma M A Mohamed, Fatma Y A Abbas, Fatma M Helbawi","doi":"10.55133/eji.320209","DOIUrl":"https://doi.org/10.55133/eji.320209","url":null,"abstract":"Systemic sclerosis (SSc) is an autoimmune disorder marked by excessive fibrosis, microvascular stenosis, and systemic clinical manifestations. An autoimmune process is believed to induce T-cell activation, mainly CD 4 T helper cells, and enhance production of proinflammatory and profibrotic cytokines such as interleukin (IL) 4 and IL 13. These cytokines contribute to vasculopathy and excessive collagen synthesis. UBASH3a (Ubiquitin Associated and SH3 Domain Containing A) and TIGIT (T cell immunoglobulin and ITIM domain) have an important role in limiting unwarranted T cell activation and maintaining immune tolerance. Our study aimed to explore UBASH3A and TIGIT mRNA expression levels in 30 SSc patients compared to 30 age and sex matched normal controls. We measured mRNA levels via real-time quantitative reverse transcription polymerase chain reaction in total RNA isolated from the peripheral blood mononuclear cells (PBMCs) of SSc patients and controls. We used RNA extraction commercial kits. The expression levels of UBASH3A and TIGIT mRNA were significantly higher in PBMCs from SSc patients compared to control subjects.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 2","pages":"92-101"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144049145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of genetic variants of TCF7L2 gene with gestational diabetes mellitus. TCF7L2基因变异与妊娠期糖尿病的关系

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2025-04-01 DOI: 10.55133/eji.320208

Nashwa R Hassan, Basma M Abdelaziz, Ahmed Aboelroose, Hasnaa Azab, Sara A Aboelros

{"title":"Association of genetic variants of TCF7L2 gene with gestational diabetes mellitus.","authors":"Nashwa R Hassan, Basma M Abdelaziz, Ahmed Aboelroose, Hasnaa Azab, Sara A Aboelros","doi":"10.55133/eji.320208","DOIUrl":"https://doi.org/10.55133/eji.320208","url":null,"abstract":"Gestational diabetes mellitus (GDM) is a metabolic disorder that poses significant risks during pregnancy. The TCF7L2 gene, previously linked to type 2 diabetes, has also been associated with GDM. This study investigated the frequency of TCF7L2 single nucleotide polymorphism (SNP) alleles and their correlation with metabolic parameters in women with GDM. The study, evaluated the TCF7L2 intron polymorphisms rs7903146 and rs11196205 in 45 GDM patients and 45 control pregnant females. GDM patients, with mean age of 30.5 ± 4.6 years, had lower sex hormone-binding globulin and serum magnesium levels than controls. The study found significant associations between GDM and both SNPs. For rs7903146, the TT genotype increased GDM risk by 19.6-fold, while the CT+TT genotypes increased the risk by 8.2-fold under the dominant genetic model. The T allele raised GDM risk by a factor of 9.3. For rs11196205, the GC and CC genotypes increased GDM risk by 12.6-fold and 10.7-fold, respectively. Under the dominant genetic model, the G/C or C/C genotypes increased GDM risk by 11.7-fold, and the C allele raised GDM risk by a factor of 8.9. In conclusion, compared to the control group, the GDM of the study individuals was substantially related to the (rs7903146) (T>C) and (rs11196205) C>G SNPs.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 2","pages":"80-91"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of liposomal amphotericin B in treating fungal meningitis in AIDS Patients: A review article. 两性霉素B脂质体治疗艾滋病真菌性脑膜炎疗效综述。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2025-01-01

Sariya Khan, Husna I Thalib, Ayesha Jamal, Zainab M A Takroni, Mohammed A Alfaqih, Manal El Said

{"title":"Efficacy of liposomal amphotericin B in treating fungal meningitis in AIDS Patients: A review article.","authors":"Sariya Khan, Husna I Thalib, Ayesha Jamal, Zainab M A Takroni, Mohammed A Alfaqih, Manal El Said","doi":"","DOIUrl":"","url":null,"abstract":"Cryptococcal meningitis is an alarming fungal infection that usually affects the meninges surrounding the brain and spinal cord. The causative organism is Cryptococcus neoformans. Although this infection can occur in normal individuals, it is more often seen in patients with human immunodeficiency virus/acquired immunodeficiency syndrome. Amphotericin B is an antifungal medication often used to treat severe fungal infections. It belongs to the class of polyene antifungal drugs, and it acts by binding to the cell membrane of the fungus. This causes some essential cellular components to leak out and ultimately the fungus dies. However, the administration of Amphotericin B is associated with toxicity. Therefore, lipid formulations are preferred to decrease the toxicity and increase the therapeutic index of the drug. It is widely used since it has a longer tissue half-life, the drug induced toxic effects are lower and it can penetrate the brain tissue efficaciously. This review collects and analyzes several research studies and literature reviews found in the electronic databases. The inclusion criteria prioritize studies focusing on the efficacy and drawbacks of using liposomal Amphotericin B as a treatment for fungal meningitis. In conclusion, liposomal Amphotericin B showed more effective treatment compared to other available antifungal drugs. Patients treated with a single dose of liposomal Amphotericin B coupled with fluconazole and flucytosine exhibited fewer adverse events and the mortality rate was also lower as compared to the control group.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 1","pages":"27-41"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of STAT4 in Multiple Sclerosis patients by polymerase chain reaction and flowcytometry. 聚合酶链反应与流式细胞术检测多发性硬化症患者STAT4。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2025-01-01

Dina A Mohareb, Ahmad K Mostafa, Mai M Nosair, Hamdy N El-Tallawy, Dina Zamzam, Shima G Mansor, Marwa A Dahpy, Randa A El Zohne

{"title":"Detection of STAT4 in Multiple Sclerosis patients by polymerase chain reaction and flowcytometry.","authors":"Dina A Mohareb, Ahmad K Mostafa, Mai M Nosair, Hamdy N El-Tallawy, Dina Zamzam, Shima G Mansor, Marwa A Dahpy, Randa A El Zohne","doi":"","DOIUrl":"","url":null,"abstract":"Multiple sclerosis (MS) is a disease of the central nervous system, characterized by progressive demyelination and inflammation. MS is characterized by immune system attacks on the myelin sheath surrounding nerve fibers. Genome-wide association studies revealed a polymorphism in the signal transducer and activator of transcription 4 (STAT4) gene that increases risk for MS. This polymorphism affects the T helper1 (Th1) cells to secrete the cytokine interferon-gamma when stimulated by interleukin (IL)-12. This study aimed to determine the association of MS active disease with STAT4 genotypes, detected by the polymerase chain reaction (PCR) and STAT4 protein level detected by flowcytometry. The study included 80 MS patients, and 70 controls matched for age and gender. We used the restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR) and flowcytometry to detect STAT4 gene polymorphism. Our results showed that, in MS patients, STAT4 genotypes of GC and CC as detected by PCR, were more common when compared to controls. The C allele of the STAT4 gene was also more common in MS patients than controls. The STAT4 GC genotype was associated with MS disease activity. Active MS patients also had a much greater frequency of the STAT4 C allele than the G allele or the control group. The STAT4 proteins level by flowcytometry among the active MS studied patients was higher than its level in the inactive patients and controls. In conclusion, this study demonstrated that both techniques are complementary to each other to detect STAT4 level in MS patients and its association with the disease activity. STAT4 proteins expression detected by flowcytometry in the peripheral blood leukocytes could be used as a biomarker for monitoring disease activity in MS patients.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 1","pages":"116-128"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of serum soluble transferrin receptor in adult Egyptian aplastic anemia patients. 埃及成年再生障碍性贫血患者血清可溶性转铁蛋白受体的测定。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2025-01-01

Rana G Abdelfatah, Gehan M Kamal, Dina M Abdo, Fady S Kamal, Haydi S Mohamed

{"title":"Assessment of serum soluble transferrin receptor in adult Egyptian aplastic anemia patients.","authors":"Rana G Abdelfatah, Gehan M Kamal, Dina M Abdo, Fady S Kamal, Haydi S Mohamed","doi":"","DOIUrl":"","url":null,"abstract":"Aplastic Anemia (AA) is one of the life-threatening bone marrow failure syndromes. One of the main pathologies of AA is reduced erythropoietic activity evidenced by decreased soluble transferrin receptor (sTfR) levels which results in minimal iron utilization and accumulation of iron in tissues in the form of ferritin. This study aimed to measure serum level of sTfR in adult AA patients and correlate it with the severity of the disease and the response to treatment. The study included 35 randomly selected AA patients recruited from the Hematology Department, aged from 17 to 66 years and 27 normal controls of matched age and sex. The level of sTfR was measured by using an enzyme linked immunoassay. The median level of sTfR was significantly lower in AA cases than in controls (17.9 nmol/l, ranged 9.87-30.42 nmol/l vs. 52.2 nmol/l; ranged 29.74-86.84 nmol/l, (p < 0.001). The concentration of sTfR was significantly lower in the Very Severe Aplastic Anemia (VSAA) patients in comparison to Severe Aplastic Anemia (SAA) 34.27 nmol/l (ranged 31.05 - 45.41 nmol/l) vs. 55.15 nmol/l (ranged 48.78-63.88 nmol/l), respectively, p= 0.004). The level of sTfR in responders to immunosuppressive treatment did not show any difference in comparison to non-responders [55.15 nmol/l (ranged 47.36 - 65.35 nmol/l) vs. 48.26 nmol/l (ranged 34.62 - 60.39 nmol/l), (p=0.808). In conclusion, sTfR level was significantly lower in AA cases than controls. The sTfR concentration expresses the erythropoietic activity in AA patients and can be an indicator of severity of bone marrow failure.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 1","pages":"42-49"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of circulating Treg and plasma microRNA-21 with rheumatoid arthritis progression. 循环Treg和血浆microRNA-21与类风湿关节炎进展的关系

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2025-01-01

Mariam Gamal, Ola El-Diwany, Sally S Abd Elhamed, Ahmed Elshafei, Reham Hammad

{"title":"Association of circulating Treg and plasma microRNA-21 with rheumatoid arthritis progression.","authors":"Mariam Gamal, Ola El-Diwany, Sally S Abd Elhamed, Ahmed Elshafei, Reham Hammad","doi":"","DOIUrl":"","url":null,"abstract":"The etiology of rheumatoid arthritis (RA) is multifaceted. One of the hypothesized pathways that results in the progression of RA is regulatory T cell (Treg) dysfunction. The pro-osteoclastogenic and immunogenic characteristics of microribonucleic acid (microRNA)-21 (miR-21) suggest its role in RA progression. Hence, we investigated the significance of plasma miR-21 and Treg cell frequency as biomarkers for RA progression and assessed the link between miR-21 and Treg frequency in RA. This study enrolled 60 RA patients classified according to disease activity score 28-joint count with erythrocyte sediment rate (DAS28-ESR) to inactive cases (n = 30) and active cases (n = 30). Flow cytometer was used to assess Treg frequency. The Real-time quantitative PCR was used to measure the expression levels of miR-21 in plasma. When compared to the inactive group, the active group revealed significant up-regulation of miR-21 expression (p = 0.004) and down-regulation of Treg frequency (p < 0.001). While Treg frequency was negatively correlated, miR-21 fold change was positively correlated with DAS-28-ESR (r = -508, p < 0.001 and r = 0.334, p < 0.009, respectively). No correlation was detected between mirR-21 and Treg frequency. Treg distinguished the two groups at area under the curve (AUC) of 0.907 with 86.7% sensitivity and 73.3% specificity, whereas miR-21 up-regulation discriminated active from inactive RA patients at AUC of 0.717, with 83.3% sensitivity and 53.3% specificity. In conclusion, Treg frequency and miR-21 fold were differentially linked to DAS-28-ESR in RA. MiR-21 fold up-regulation changes and Treg frequency down-regulation can be suggested as biomarkers for RA activity.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 1","pages":"63-76"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulation of proinflammatory cytokines and dynamic expression of TGF-β1 molecules induced by anti-IL-17 mAb in murine schistosomiasis mansoni. 抗il -17单抗诱导小鼠曼氏血吸虫病促炎细胞因子下调及TGF-β1分子动态表达

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2025-01-01

Mostafa E Mostafa, Morsy R M Geneedy, Ahmed M A Mohamed, Mohamed E Abo-Mandil, Fatma M El-Lessy

{"title":"Downregulation of proinflammatory cytokines and dynamic expression of TGF-β1 molecules induced by anti-IL-17 mAb in murine schistosomiasis mansoni.","authors":"Mostafa E Mostafa, Morsy R M Geneedy, Ahmed M A Mohamed, Mohamed E Abo-Mandil, Fatma M El-Lessy","doi":"","DOIUrl":"","url":null,"abstract":"Hepato-intestinal schistosomiasis is characterized by severe pathological changes at advanced chronic stages, including granulomatous lesions and liver fibrosis. The objective of our research was to assess the dynamic expression of profibrotic molecules, the transforming growth factor beta 1 (TGF-β1), and proinflammatory cytokines immunomodulation induced by interleukin 17 (IL-17) neutralization in murine Schistosomiasis mansoni. The study included 56 specific pathogen-free male C57BL/6 mice, divided into 3 main groups: GI uninfected normal controls, GII S. mansoni infected with 70±5 cercariae/non-treated, GIII S. mansoni infected and treated with anti-IL-17 monoclonal antibody (mAb), GIV S. mansoni infected and isotype-matched IgG2a mAb was given as a challenge. Mice were sacrificed at 6, 8, and 10 weeks after infection, then their liver enzymes and cytokines assessed, histopathological and immunohistochemical tested. The present study demonstrated a statistically significant elevation in serum levels of IL-17 (p < 0.01), TGF-β1 (p < 0.01), IL-1β (p≤0.001), IL-4 (p≤0.003), IL-6 (p≤0.05), and liver enzymes (ALT: p≤0.001; AST: p≤0.002). Additionally, granulomatous lesions and TGF-β1 expression were significantly increased (p < 0.001) in infected mice at 6, 8, and 10 weeks after infection. All showed significant reduction by neutralization with anti-IL-17 mAb. Finally, IL-17 exhibited potent profibrogenic activity, and anti-IL-17 mAb can be used to alleviate and counteract this impact in murine S. mansoni.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 1","pages":"16-26"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using flow-cytometry in measuring platelet activity in type 2 diabetes and predicting macrovascular complications. 用流式细胞术测定2型糖尿病患者血小板活性及预测大血管并发症。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2025-01-01

Mai M Aly, Hanaa M Mohamed, Fatema A El-Osily, Eman R Badawy, Mohamed M Shehab, Mohammad H AbdEllah-Alaui, Dina A Hamad

{"title":"Using flow-cytometry in measuring platelet activity in type 2 diabetes and predicting macrovascular complications.","authors":"Mai M Aly, Hanaa M Mohamed, Fatema A El-Osily, Eman R Badawy, Mohamed M Shehab, Mohammad H AbdEllah-Alaui, Dina A Hamad","doi":"","DOIUrl":"","url":null,"abstract":"Platelets are hyperactive in patients with type2 diabetes (T2DM), they adhere to vascular endothelium and play a key role in macrovascular complications. Platelets activity can be measured by flow-cytometry (cluster of differentiation (CD) 41, CD 42, CD 62, CD 63), which allows detection of surface antigens in a sensitive and specific manner. This study aimed to describe platelets activity in T2DM in association with cardiovascular and cerebrovascular complications in relation to duration of diabetes (DM). This was a case-control study with 130 participants (65 diabetic cases and 65 normal controls). All cases were subjected to history and clinical examination, base-line laboratory investigations and surface expression of platelets receptors e.g. CD 41% and mean fluorescent intensity (MFI), CD 42% and MFI, CD 62% and MFI, CD 63% and MFI were determined by flow-cytometry. There was a statistically significant higher expression of CD 62%, CD 62 MFI, CD 63% and CD 63 MFI (p < 0.001 for all) in diabetic cases compared to controls. There were significantly higher CD 62 %, CD 62 MFI, CD 63% and CD 63 MFI in cases with cardiovascular complications (p=0.001, p < 0.001, p=0.05 and p=0.007, respectively) and in cases with cerebrovascular complications compared to cases without complications (p=0.05, p=0.008, p=0.035, p=0.017, respectively). A significant positive correlation was found between glycated hemoglobin, body mass index and CD 62 %, CD 62 MFI and CD 63%. Using the receiver operating characteristic curve showed that CD 62 %, CD 62 MFI, CD 63 % and CD 63 MFI have a diagnostic ability to early predict DM (area under the curve (AUC)=0.998) as well as cardiovascular (AUC=0.855) and cerebrovascular (AUC=0.765) complications. In conclusion CD 62%, CD 62 MFI, CD 63% and CD 63 MFI markers have a diagnostic ability for early prediction of cardiovascular and cerebrovascular complications among diabetic patients.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 1","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0