The Egyptian journal of immunology / Egyptian Association of Immunologists最新文献_第2页

Effectiveness of zero dose HBV vaccine on prevention of HBV breakthrough infection among vaccinated Egyptian children. 零剂量 HBV 疫苗对预防接种疫苗的埃及儿童出现 HBV 突破性感染的效果。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2024-10-01

Asmaa M El-Nasser, Amany M Tawfeik, Eman A E Abushady, Eman A Mohammad, Marwa Elhady, Abeer M Abdul-Mohymen, Salah E I Ali

{"title":"Effectiveness of zero dose HBV vaccine on prevention of HBV breakthrough infection among vaccinated Egyptian children.","authors":"Asmaa M El-Nasser, Amany M Tawfeik, Eman A E Abushady, Eman A Mohammad, Marwa Elhady, Abeer M Abdul-Mohymen, Salah E I Ali","doi":"","DOIUrl":"","url":null,"abstract":"Hepatitis B virus (HBV) is a vaccine preventable disease. Sufficient post vaccination response is critical step to achieve infection eradication. Vaccine hypo-responsiveness is a major risk factor for HBV chronic infection. We aimed to evaluate the effectiveness of birth dose HBV vaccine in preventing perinatal HBV infection and to detect the rate of HBV surface antibody (HBs-Ab) seroconversion and its relation to interleukin-4 polymorphism (IL-4 PM) among a group of vaccinated Egyptian infants. This observational analytical study involved 77 infants aged 6 to 12 months who received 4 doses of HBV vaccine including a zero dose. We measured serum levels of HBV-DNA and hepatitis B surface antigen (HBsAg) as markers of infectivity, and the level of (HBsAb) to assess vaccine responsiveness. Cytokine gene analysis to detect IL-4 gene polymorphism and its association with vaccine un-responsiveness were investigated. We observed that none of the vaccinated infants acquired HBV infection. Of the included 77 infants, seroconversion against HBV was detected in 72 (93.5%), 28 (36.4%) had low response and 44 (57.1%) had high response. While 5 (6.5%) were non responders. There was significant association between IL-4 gene polymorphism and the poor seroconversion after HBV vaccination. (p=0.03). Furthermore, HBsAb titer was significantly lower in children who have IL-4 gene polymorphism (p=0.014). In conclusion, implementation of birth-dose HBV vaccination is effective for prevention of perinatal infection, but seroconversion rate may be insufficient to induce long term protection. IL-4 gene polymorphism is associated with poor response to HBV vaccine.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"31 4","pages":"66-75"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knowledge, attitude, and practice toward rheumatic fever and rheumatic heart disease among parents in Al-Baha Region, Saudi Arabia: A Cross-Sectional study. 沙特阿拉伯巴哈地区家长对风湿热和风湿性心脏病的认识、态度和做法：一项横断面研究。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2024-10-01

Khalid A A Mohammed, Elfatih M M Salih, Fahad A Alamr, Abdullah S Alghamdi, Hanan M Alghamdi, Majed A Alghamdi, Mohammed H M Alzahrani, Mashael M A Alzahrani, Omar K Alzahrani

{"title":"Knowledge, attitude, and practice toward rheumatic fever and rheumatic heart disease among parents in Al-Baha Region, Saudi Arabia: A Cross-Sectional study.","authors":"Khalid A A Mohammed, Elfatih M M Salih, Fahad A Alamr, Abdullah S Alghamdi, Hanan M Alghamdi, Majed A Alghamdi, Mohammed H M Alzahrani, Mashael M A Alzahrani, Omar K Alzahrani","doi":"","DOIUrl":"","url":null,"abstract":"Rheumatic fever (RF) is an autoimmune inflammatory process that develops as a sequela of untreated group A streptococcal pharyngitis and primarily affects children. It can lead to rheumatic heart disease (RHD), a major cause of cardiovascular morbidity and mortality. Awareness and preventive measures are crucial to mitigate its impact, particularly in low-resource settings. This study aimed to assess the knowledge, attitudes, and practices toward RF and RHD among parents living in Al-Baha Region. And to determine its association with other different sociodemographic variables. This was a cross-sectional study included 415 parents in Al-Baha region, Saudi Arabia. using a self-administered questionnaire distributed online. Of the total 415 participants, more than half were females 223 (53.7%), and 149 (35.9%) participants aged between 45 and 55 years. The knowledge regarding RF was classified as good 20 (4.8%), fair 104 (25.1%), and bad 291 (70.1%). The attitude towards RF showed that about half of the participants 206 (49.7%) had a negative attitude, 169 (40.7%) natural and 40 (9.6%) positive attitudes. While the total practice level was classified as Bad 15 (3.6%), Fair 113 (27.2%) and Good 287 (69.2%), females were more likely than males to exhibit good practices (p = 0.001). The study concluded that knowledge about RF and RHD among parents in the Al Baha region, Saudi Arabia, is very poor. Most participants lacked sufficient understanding of the definition of RF, its relationship with bacterial sore throats, prophylactic treatments, and the vulnerable age group. Attitudes towards RF were relatively negative. Therefore, healthcare authorities and providers should raise awareness and create educational programs to improve public understanding of RF and RHD, aiding in their prevention and control in the Al Baha region.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"31 4","pages":"123-135"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A study on serum zonulin in chronic spontaneous urticaria patients. 关于慢性自发性荨麻疹患者血清zonulin的研究。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2024-10-01

Sylvia T Kamal, Mohamed A Elshayeb, Menna Allah Z Abou Elwafa, Radwa H A ElAdawy, Mariam A Mohamed, Osama M Abdel Latif

{"title":"A study on serum zonulin in chronic spontaneous urticaria patients.","authors":"Sylvia T Kamal, Mohamed A Elshayeb, Menna Allah Z Abou Elwafa, Radwa H A ElAdawy, Mariam A Mohamed, Osama M Abdel Latif","doi":"","DOIUrl":"","url":null,"abstract":"Chronic spontaneous urticaria (CSU) is a widespread disease with a complicated heterogenous pathophysiology. Increased intestinal permeability i.e., leaky gut has been linked to the pathology of many diseases. Zonulin was recently used as a marker for leaky guts. This study aimed to assess the relation between serum zonulin level and CSU and its possible relationship with disease activity. This was a comparative cross-sectional study, which included 97 CSU adult patients and 87 apparently healthy controls. CSU patients had significant lower zonulin level than controls (p < 0.001). The median of serum zonulin level was equal to 2.93 ng/ml with interquartile range (IQR) (1.40-4.19) in the CSU group and of 3.92 ng/ml with IQR (2.97-4.69) in the control group. We found a positive correlation between serum zonulin and C-reactive protein with Pearson correlation coefficient of 0.2, (p=0.04). No significant correlation was found between serum zonulin level and urticaria activity score 7 or total immunoglobulin E level. In conclusion, this study found that serum zonulin level is lower in CSU patients than in controls which could be attributed to food restriction, severity of the CSU disease and/or drug intake in the CSU cases.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"31 4","pages":"98-107"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic and immunological implications in fibromyalgia: A Literature Review. 纤维肌痛的遗传和免疫学影响：文献综述。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2024-10-01

Sariya Khan, Husna I Thalib, Dana Kauther, Ayesha Jamal, Adnan A Moallem, Samer S Aldera, Anisa Abou Touk, Fatma E Hassan

{"title":"Genetic and immunological implications in fibromyalgia: A Literature Review.","authors":"Sariya Khan, Husna I Thalib, Dana Kauther, Ayesha Jamal, Adnan A Moallem, Samer S Aldera, Anisa Abou Touk, Fatma E Hassan","doi":"","DOIUrl":"","url":null,"abstract":"Fibromyalgia (FM), a musculoskeletal condition characterized by widespread pain and numerous associated symptoms, is a complex disorder with uncertain etiology and pathogenesis. Most of the patients suffering from this syndrome are undiagnosed due to a lack of standard diagnostic criteria. Recent studies have shown the involvement of immune dysfunction and various pro-inflammatory cytokines in FM. Since there is so much uncertainty regarding the pathogenesis of FM, treatment modalities are very limited and ineffective. This review aimed to analyze the immunological mechanisms behind FM, attempting to deepen the understanding of its pathogenesis. Additionally, the review elucidates FM's associations with autoimmune diseases, highlighting shared pathophysiological mechanisms and overlapping symptoms. We synthesized current literature available on Google Scholar, PubMed, Springer, and Web of Science, the review explored the intricate interactions between genetic predisposition, immune dysregulation, and environmental factors in FM pathogenesis. The inclusion criteria prioritized studies focusing on the immunological aspect of FM. In conclusion, immune dysfunction has a role to play in the pathogenesis of FM, and immunomodulatory therapies have proven to be beneficial in the treatment of FM. Genetic variants, epigenetic modifications, and gut microbiome alterations are potential triggers for immune system dysfunction, contributing to the manifestation and exaggeration of FM symptoms. This review provided a comprehensive resource for researchers and clinicians, a guide for future investigations and clinical management towards improved outcomes and enhanced quality of life for individuals with FM.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"31 4","pages":"13-26"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological and hormonal changes between postmenopausal rheumatoid arthritis and systemic lupus erythematosus in women. 妇女绝经后类风湿性关节炎和系统性红斑狼疮之间的生理和激素变化。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2024-10-01

Luma Q Ali, Firas S Abdulhadi, Ban Talib El-Haboby, Jamela Jouda

{"title":"Physiological and hormonal changes between postmenopausal rheumatoid arthritis and systemic lupus erythematosus in women.","authors":"Luma Q Ali, Firas S Abdulhadi, Ban Talib El-Haboby, Jamela Jouda","doi":"","DOIUrl":"","url":null,"abstract":"It is thought that sex hormones are playing an actual role in the pathogenesis of rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The purpose of this study was to determine the differences of some hematological, hormonal and immunological parameters between the postmenopausal women with rheumatoid arthritis and systemic lupus erythematosus. This study contained 75 postmenopausal women (52-65 years old). They included 25 women diagnosed with rheumatoid arthritis, 25 diagnosed with systemic lupus erythematosus and 25 normal controls. Blood was collected and used to determine complete blood count (CBC) and erythrocyte sedimentation rate (ESR) tests. Serum was separated and used to determine Follicle-stimulating hormone (FSH), Luteinizing Hormone (LH), Prolactin, cortisol, Estrogen, progesterone, testosterone, rheumatoid factor (RF) and Immunoglobulin E (IgE). The results indicated that both patients' groups had anemia. However, the RA group had leukocytosis, but the SLE group had leukopenia. Moreover, thrombopenia occurred only in SLE patients. Although the levels of FSH and cortisol were significantly higher, the levels of LH, Estrogen and testosterone had significantly dropped in both patient groups. Interestingly, the level of progesterone was higher in the SLE and lower in the RA group. Moreover, the levels of RF, ESR and IgE were significantly increased in both patients' groups. In conclusion, there were many differences in hematological and hormonal levels between postmenopausal women with rheumatoid arthritis and systemic lupus erythematosus. Such findings need future work to find out the reasons for these differences and how they could be used in future treatment strategies.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"31 4","pages":"76-84"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of IL-13 rs20541, FOXP3 rs3761548 genes polymorphisms and serum level of IL-13 with allergic asthma in Egyptian patients. 埃及患者 IL-13 rs20541、FOXP3 rs3761548 基因多态性及血清 IL-13 水平与过敏性哮喘的关系。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2024-07-01

Ahmed R M Hassan, Amira G M Abdallah, Nagwan A Ismail, Yasmin A Fahmy

{"title":"Association of IL-13 rs20541, FOXP3 rs3761548 genes polymorphisms and serum level of IL-13 with allergic asthma in Egyptian patients.","authors":"Ahmed R M Hassan, Amira G M Abdallah, Nagwan A Ismail, Yasmin A Fahmy","doi":"","DOIUrl":"","url":null,"abstract":"The interleukin 13 (IL-13) gene single nucleotide polymorphisms (SNPs) are frequently linked to increased vulnerability to allergic asthma. Forkhead box protein P3 (FOXP3) is an important molecule in the formation of regulatory T cells (Treg). The genetic variants that alter FOXP3 function may have a role in the development of asthma and other allergic disorders. We aimed to determine the association of IL-13 rs20541, FOXP3 rs3761548 genes SNPs and serum levels of IL-13 with allergic asthma patients. In this case-control study, 41 Egyptian patients with allergic asthma were included. Age and gender matched. 41 normal volunteers were considered the controls. All subjects were examined for IL-13 rs20541 and FOXP3 rs3761548 SNPs by the polymerase chain reaction /restriction fragment length polymorphism technique. The serum level of IL-13 was assessed by the enzyme linked immunosorbent assay (ELISA). AA genotype at IL-13 rs20541 SNP was statistically significantly different between the studied groups (p= 0.042). Also, a statistically significant difference was detected when compared AA genotype to GG genotype as AA genotype was three times at risk for asthma (p1=0.031) (OR=3.95) and A allele increased the risk of asthma by about 3 times (OR=3.2). AA genotype at FOXP3 rs3761548 SNP was statistically significantly different between the studied groups (p=0.013). Also, a statistically significant difference was detected when compared AA genotype to CC genotype as AA genotype was 7 times at risk for asthma (p1=0.003) (OR=7.04) and A allele increased the risk of asthma by about 3 times (p<0.001) (OR=3.07). The serum level of IL-13 was statistically significant different between both groups (p<0.001). We can conclude that IL-13 could be a useful tool for predicting allergic asthma. Patients with AA genotype of IL-13 rs20541 and AA genotype of FOXP3 rs3761548 have a higher risk for developing allergic asthma.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"31 3","pages":"15-27"},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunostimulant effect of dates (Phoenix dactylifera) on humoral and cellular immunity cells and their functions. 红枣（Phoenix dactylifera）对体液和细胞免疫细胞及其功能的免疫刺激作用。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2024-07-01

Soukaina Laaraj, Ibtissam Ouahidi, Nada Al Moudani, Abdellatif Boukir, Samira Jaouhar, Lotfi Aarab

{"title":"Immunostimulant effect of dates (Phoenix dactylifera) on humoral and cellular immunity cells and their functions.","authors":"Soukaina Laaraj, Ibtissam Ouahidi, Nada Al Moudani, Abdellatif Boukir, Samira Jaouhar, Lotfi Aarab","doi":"","DOIUrl":"","url":null,"abstract":"The study aimed to assess the immunomodulatory effects of Phoenix dactylifera (dates) fruit, a traditional remedy used by Moroccans to enhance immunity against pathogens. This research sought to evaluate the impacts of this fruit on immune cells and their functions. To achieve this, we conducted tests using date extracts on splenocytes, thymocytes, and macrophages, focusing on their functions: antibody production, phagocytosis, and T-lymphocyte toxicity. The results obtained demonstrated that the aqueous extract of P. dactylifera fruit exhibited significant immunostimulatory effects on humoral immunity. It achieved this by enhancing complement activity and increasing splenocyte (including B-lymphocytes) proliferation by 142.5% compared to control cells. Similarly, in the same conditions, there was notable stimulation of cellular immunity through thymocyte activity, resulting in a remarkable increase in cell proliferation (225%) and a boost in thymocyte function (245.9%), which plays a role in safeguarding against cancer. Moreover, the date extract demonstrated anti-inflammatory properties. This was evident in the increased phagocytosis activity mediated by macrophages under the ethyl acetate extract, effectively eliminating pathogens. Assessing the cosmetic potential of date extracts showed that the ethyl acetate extract possesses both anti-inflammatory and strong antioxidant effects, exhibited high photo absorption of ultraviolet-B rays. Based on these findings, we propose to study the utilization of this extract for sun protection as a sunscreen. Furthermore, the Fourier-transform infrared spectroscopy analysis indicated that the most active compounds present were flavonoids. These outcomes substantiate the traditional usage of this fruit for reinforcing immunity.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"31 3","pages":"28-40"},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Helicobacter pylori present in caries sample among dental caries patients. 龋齿患者的龋齿样本中存在幽门螺旋杆菌。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2024-07-01

Zainab K Ahmad, Baha H H Al-Amiedi, Thulficar G H Al-Khafaji, Sinan A Shwailiya

{"title":"Helicobacter pylori present in caries sample among dental caries patients.","authors":"Zainab K Ahmad, Baha H H Al-Amiedi, Thulficar G H Al-Khafaji, Sinan A Shwailiya","doi":"","DOIUrl":"","url":null,"abstract":"Helicobacter pylori is Gram negative bacteria, the reason for causing peptic ulcer. There is suggestion between the presence of H. pylori in oral cavity and gastritis. The present study aimed to detect H. pylori in dental caries samples. The study included 29 dental caries patients from both sexes (13 males and 16 females), with different age groups (children and adult), and nine apparently healthy subject as a control group (2 males & 7 females). Dental caries samples were collected and investigated for this study from patients with dental caries who visited the Dental Faculty in the College of Dentistry, University of Babylon, Iraq. H. pylori antigen was detected using an enzyme linked immunosorbent assay (ELISA) technique. Of the 29 dental caries patients, 19 (65.51%) patients were positive for H. pylori antigen test. Most of them were in the age group 20-30 (9 patients) & 30-40 (8 patients). The age groups (10-20) & (40-50) years shows 100% positivity for H. pylori antigen. Also, result was recorded significant higher difference's between H. pylori positive antigen between dental caries patients and H. pylori positive antigen among control group. (t=2.697,df=5, p≤ 0.05). Pearson correlation recorded significantly higher association between the presence of H. pylori antigen and the dental caries infection among test group (r=1, p≤ 0.000), 4 (44.5%) of the 9 control subjects, without dental caries, were positive for H. pylori antigen test. In summary, the H. pylori positive antigen test was recorded in both dental caries patients (65.51%) and in the control group (62.5 %). In conclusion, H. pylori antigen was present in dental caries patients. This could indicate that the bacteria H. pylori present in dental caries samples may contribute to caries processes.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"31 3","pages":"41-47"},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MicroRNA-155 is a potential predictive tool for atopic dermatitis severity in children: A preliminary study. MicroRNA-155 是预测儿童特应性皮炎严重程度的潜在工具：初步研究

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2024-07-01

Lobna A El-Korashi, Ola E Nafea, Alaa E Nafea, Basma M Elkholy, Lamia L Elhawy, Amina A Abdelhadi

{"title":"MicroRNA-155 is a potential predictive tool for atopic dermatitis severity in children: A preliminary study.","authors":"Lobna A El-Korashi, Ola E Nafea, Alaa E Nafea, Basma M Elkholy, Lamia L Elhawy, Amina A Abdelhadi","doi":"","DOIUrl":"","url":null,"abstract":"Atopic dermatitis (AD) is one of the most prevalent chronic inflammatory dermatological disorders in childhood. Assessment of AD severity is the initial step in designing the proper therapeutic plan. Moreover, it is imperative for evaluation of disease improvement during and following therapy. This study was designed to assess the prognostic role of miRNA-155 (miR-155) in the prediction of AD severity as the primary outcome. While the secondary outcome was to correlate the serum miR-155 expression levels with the scoring atopic dermatitis (SCORAD) severity index. This case-control study included 24 children with AD and 24 apparently healthy children as a control group. AD children were stratified according to the SCORAD severity index. Approximately 58% of children had mild AD, 25% moderate AD, and about 17% severe AD. Children with AD had statistically significantly higher miR-155 expression levels in comparison to the control children, (p < 0.001). Children with severe AD had statistically significantly higher miR-155 expression levels compared to mild AD children (p=0.001). The receiver operating characteristic curve analysis for miR-155 demonstrated that miR-155 can differentiate between children with mild AD and those with moderate-to-severe AD, with an area under the curve of 0.879, and an excellent discrimination power. A statistically strong significant positive correlation existed between miR-155 levels and SCORAD severity index (rs= 0.666, p < 0.001). In conclusion, MiR-155 could be considered as a non-invasive biomarker of AD severity in children. It is a promising prognostic tool in the prediction of AD severity.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"31 3","pages":"131-139"},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141601957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Profiles of miRNA expression and IFN-γ serum level as biomarker for the development of Multiple Sclerosis. 作为多发性硬化症发病生物标志物的 miRNA 表达谱和 IFN-γ 血清水平。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2024-07-01

Baydaa M Abaas, Mayyada F Darweesh

{"title":"Profiles of miRNA expression and IFN-γ serum level as biomarker for the development of Multiple Sclerosis.","authors":"Baydaa M Abaas, Mayyada F Darweesh","doi":"","DOIUrl":"","url":null,"abstract":"Multiple sclerosis (MS) is associated with a wide spectrum of sensory, motor, and psychological disorders. Cytokines level and microRNA (miRNA) expression have roles in the disease's progression and the start of a damaging immune response in the central nerve system. This research study aimed to determine the role of interferon-γ (IFN-γ) and microRNA-326 (MiR-326) as prognostic factors for the development of MS disease in relation to different treatments. This case-control study included 100 participants, classified as 80 MS patients and 20 apparently healthy subjects as a control group. IFN-γ level was determined by an enzyme linked immunosorbent assay. The expression level of micR326 was determined by the reverse transcription polymerase chain reaction technique. The mean level of serum IFN-γ in MS patients (102.83 ± 15.79 ng/ml) was significantly higher than in the control group (61.25 ± 12.51 ng/ml) (p=0.001). A higher concentration of IFN-γ was observed in the secondary progressive form of MS disease relative to relapsing-remitting multiple sclerosis (RRMS) and in comparison, with the controls group, this IFN-γ cytokine level was significantly higher in treatment-naive patients. There was an increase in the mean fold change of miRNA-326 expression in patients (3.1 ±1.65) compared to the control group (1.03 ±0.23). In conclusion, secondary progressive multiple sclerosis (SPMS) has higher IFN-γ serum level than RRMS. MiR-326 may participate in the development of MS and its expression can be a useful biomarker for the prediction of MS.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"31 3","pages":"62-70"},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0