The Egyptian journal of immunology / Egyptian Association of Immunologists最新文献_第3页

Autoimmune rheumatic manifestations in a cohort of Egyptian COVID-19 patients. 埃及COVID-19患者队列中的自身免疫性风湿病表现

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2025-01-01

Dalia A ElSherbiny, Samah A El Bakry, Sara A Abd El Rahman, Amr M Hawwash

{"title":"Autoimmune rheumatic manifestations in a cohort of Egyptian COVID-19 patients.","authors":"Dalia A ElSherbiny, Samah A El Bakry, Sara A Abd El Rahman, Amr M Hawwash","doi":"","DOIUrl":"","url":null,"abstract":"The coronavirus disease 2019 (COVID-19) pandemic had significant global health impact. Like systemic autoimmune diseases, COVID-19 may manifest with systemic and heterogenous clinical presentations. This study aimed to evaluate the prevalence of autoimmune rheumatic manifestations among a cohort of Egyptian patients with COVID-19 infection. The study included 90 adult confirmed COVID-19 patients as determined by the polymerase chain reaction test. They were subjected to the following assessments: detailed medical history, full clinical and rheumatological examination, routine laboratory investigations, a panel of autoimmune markers, and high-resolution computed tomography chest. Then the patients studied were divided according to the positivity of autoimmune markers into positive and negative groups. According to the COVID-19 disease severity, patients were divided into mild, moderate, severe, and critical groups. The mean age of the study population was 54.60 ± 10.72 years, and 53.3% of them were females and 46.7% males. Of the patients studied 13.3% had positive antinuclear antibodies (ANA), 15.6% positive for rheumatoid factor (RF), 8.9% positive for anticardiolipin (ACL) IgM, and 5.6% positive for ACL IgG. The autoimmune markers were not statistically different however, all cases with positive ANA were present among severe and critical COVID-19 cases. All cases with positive RF, ACL IgM, or ACL IgG were found among moderate, severe, and critical patients. In conclusion, COVID-19 disease is associated with variable autoimmune manifestations. Autoimmune rheumatic manifestations, either clinical or autoimmune markers, are more evident in severe and critical COVID-19 cases. COVID-19 patients with positive ANA or RF are more likely to develop cutaneous, musculoskeletal, and vascular manifestations.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 1","pages":"105-115"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Clinical utility of Interleukin-17A rs2275913 polymorphism with colorectal cancer in Egyptian patients. 白介素- 17a rs2275913多态性在埃及结直肠癌患者中的临床应用

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2025-01-01

Heba H A Osman, Manal M Abd Al Aziz, Aya M M Yehia, Rawan M M Metwaly

{"title":"The Clinical utility of Interleukin-17A rs2275913 polymorphism with colorectal cancer in Egyptian patients.","authors":"Heba H A Osman, Manal M Abd Al Aziz, Aya M M Yehia, Rawan M M Metwaly","doi":"","DOIUrl":"","url":null,"abstract":"The worldwide incidence of colorectal cancer (CRC) is roughly two million new instances each year throughout the world, according to the World Health Organization 2022. CRC is the third most prevalent disease and the second most common cancer in terms of fatality. People diagnosed with colorectal cancer in the early stages have a five-year survival rate of roughly 95%, but people identified with the disease in the later stages have a survival rate of approximately 12%. There are a number of variables that contribute to the development of CRC, these factors include both hereditary and environmental influences. We aimed to investigate the clinical utility of Interleukin (IL)-17A rs2275913 polymorphism to assess its association with CRC in Egyptian patients and the ability of using it as a non-invasive biomarker to assist in diagnosis of colorectal cancer. This case-control study included 75 subjects. Of these, 35 were CRC cases, 20 inflammatory bowel disease (IBD) patients and 20 normal control persons. Blood was collected and DNA extracted. The rs2275913 of IL-17A was genotyped using the Real-Time polymerase chain reaction (PCR). In CRC patient's group, (5.71%) had the homozygous AA genotype, (40%) the heterozygous GA genotype and (54.29%) the wild GG genotype. While in IBD group (15%) had the homozygous AA genotype, (40%) the heterozygous GA genotype and (45%) the wild GG genotype. In the normal control group, no one had the homozygous AA genotype, but (45%) had the heterozygous GA genotype and (55%) the wild type of GG genotype. However, there was no statistically significant substantial variation amongst the three groups (p>0.05). In conclusion, our study demonstrated no association of IL-17A rs2275913 with both CRC and IBD in the Egyptian population.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 1","pages":"92-104"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between TLR-2 and TLR-9 gene polymorphisms (rs5743708 and rs5743836) and susceptibility to psoriatic arthritis in Egyptian patients. TLR-2和TLR-9基因多态性（rs5743708和rs5743836）与埃及患者银屑病关节炎易感性的关系

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2025-01-01

Nora E Abdelbaset, Sahar S Khattab, Nashwa M Abd Elbaky, Basma M Elnaggar, Sara A Galal

{"title":"Association between TLR-2 and TLR-9 gene polymorphisms (rs5743708 and rs5743836) and susceptibility to psoriatic arthritis in Egyptian patients.","authors":"Nora E Abdelbaset, Sahar S Khattab, Nashwa M Abd Elbaky, Basma M Elnaggar, Sara A Galal","doi":"","DOIUrl":"","url":null,"abstract":"Psoriasis (PsO) is a chronic immune-mediated disease of the skin. Psoriatic arthritis (PsA) is a prevalent chronic inflammatory disease that is associated with joint destruction and disability. The presence of PsO is the single greatest risk factor for the development of PsA. Toll-like receptors (TLRs) are trans-membrane proteins coded by the toll genes family. They are expressed in different cell types including immune and non-immune cells. Polymorphisms in TLR genes that lead to changes in these receptors or interfere with the transcription rates of their messenger ribonucleic acid (mRNA), may be involved in the chronic inflammatory immune response observed in PsA. This study involved 50 patients with PsA, 50 patients with cutaneous PsO and 50 age and sex matched normal subjects as controls. We aimed to assess TLR-2 (rs5743708) and TLR-9 (rs5743836) gene polymorphisms as potential risk factors for PsA in Egyptian patients with cutaneous PsO. Genotyping and allele frequencies were performed using Real Time polymerase chain reaction (qRT-PCR). Toll-like receptor-2 (TLR-2) rs5743708 and TLR-9 rs5743836 polymorphisms were associated with increased risk of PsO as an autoimmune disease, however they were not related to increase susceptibility to PsA in this cohort study of Egyptian patients.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 1","pages":"77-91"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lupus disease activity state and Foxp3 gene polymorphism. 狼疮疾病活动状态与Foxp3基因多态性。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2025-01-01

Hanaa I Abd El-Hady, Enas I Abdelhady, Mai A Kamel

{"title":"Lupus disease activity state and Foxp3 gene polymorphism.","authors":"Hanaa I Abd El-Hady, Enas I Abdelhady, Mai A Kamel","doi":"","DOIUrl":"","url":null,"abstract":"The autoimmune disease systemic lupus erythematosus (SLE) is presented with many clinical symptoms. The transcription factor fork head box protein 3 (Foxp3) is expressed on regulatory T (T-reg) cells and essential for its development and function. Functional single-nucleotide polymorphisms (SNPs) in the Foxp3-3279 (rs3761548 C/A) gene influence SLE pathogenesis. We aimed to assess the relation between the functional polymorphism in Foxp3-3279 (rs3761548 C/A) gene and risk of SLE development and lupus disease activity state. This case-control study included SLE patients, diagnosed according to American College of Rheumatology/Systemic Lupus International Collaborating Clinics (ACR/SLICC) classification criteria. The degree of disease activity was assessed by Systemic Lupus Erythematosus Disease Activity Score (SLE-DAS). Foxp3-3279 (rs3761548 C/A) gene polymorphism was detected using the polymerase chain reaction restriction fragment length polymorphism-based analysis (PCR-RFLP). We found that AA and AC genotypes significantly increased the risk of SLE by 7.25 and 2.88 folds, respectively (p < 0.001) and A allele significantly increased that risk by 3.12 folds (p < 0.001). AA genotype significantly increased the risk of SLE moderate-severe disease activity and risk of lupus nephritis by 33.6 folds (p < 0.001). In conclusion, Foxp3 -3279 (rs3761548 C/A) gene polymorphism was associated with the risk of SLE and lupus nephritis. The relation of this SNP with SLE disease activity highlighted the role of Foxp3 gene in SLE pathogenesis and manifestations that could potentially enhance the management of SLE patients by identifying each person's unique response to treatment.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 1","pages":"129-138"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143012775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation between IL-17 and inflammatory bowel disease. IL-17与炎症性肠病的相关性

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2025-01-01

Hiba T Majeed, Zahraa Y Motaweq

{"title":"Correlation between IL-17 and inflammatory bowel disease.","authors":"Hiba T Majeed, Zahraa Y Motaweq","doi":"","DOIUrl":"","url":null,"abstract":"Inflammatory bowel disease (IBD) is a protracted, persistent gastrointestinal disease that is distinguished by recurring, persistent inflammation of the digestive tract. IBD, including Crohn's disease and ulcerative colitis, is characterized by persistent inflammation due to immune dysregulation. Interleukin -17 (IL-17) contributes significantly to the pathophysiology of IBD, as highlighted in the context of the provided research. There is a strong correlation between IL-17 inhibitors and the onset or exacerbation of IBD, with IL-17 inhibitor treatment being linked to gastrointestinal inflammatory events such as IBD and colitis. The goal of the current study was to determine the relationship between IBD disease activity and serum IL-17 levels in individuals with IBD. This case-control study included 102 IBD patients and 48 normal individuals as a control group. Blood samples were used for measuring IL-17 levels using the enzyme-linked immunosorbent assay. Levels of IL-17 were increased in patients compared with the control group (p=0.0001). IL-17 levels were significantly elevated in patients aged 1-15 years, 31-45 years and 16-30 years. IL-17 levels showed significant differences between two types of inflammatory bowel disease being elevated in Crohn's disease more than ulcerative colitis (p=0.001). IL-17 demonstrated significant differences in IL-17 levels between male and female in patients and controls groups (p=0.0001). In conclusion, patients with IBD have higher serum levels of IL-17, indicating that these cytokines may have a role in the development and pathophysiology of Crohn's disease and ulcerative colitis. Overall, monitoring IL-17 levels could serve as valuable biomarkers for assessing IBD severity and guiding treatment decisions.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 1","pages":"56-62"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trefoil Factor 3 in human serum as a predictor of disease activity in Egyptian ulcerative colitis patients and its role in colorectal cancer. 埃及溃疡性结肠炎患者血清中作为疾病活动性预测因子的三叶草因子 3 及其在结直肠癌中的作用。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2024-10-01

Walaa M Hashem, Amir H Samy, Sarah A Ali, Christina A Anwar, Mina W N Abd El Malak, Mohamed N B Al-Ashram

{"title":"Trefoil Factor 3 in human serum as a predictor of disease activity in Egyptian ulcerative colitis patients and its role in colorectal cancer.","authors":"Walaa M Hashem, Amir H Samy, Sarah A Ali, Christina A Anwar, Mina W N Abd El Malak, Mohamed N B Al-Ashram","doi":"","DOIUrl":"","url":null,"abstract":"Ulcerative colitis (UC), a chronic idiopathic inflammatory disease, is caused by abnormal immune response to intestinal microflora. Colorectal cancer (CRC) is one of the leading causes of cancer-related mortality. The gold standard to establish diagnosis and assess disease activity remains endoscopy and histopathology. Non-invasive biomarkers are required for timely diagnosis of CRC and to assess disease activity as endoscopic assessment is not accepted by most patients. Enhanced trefoil factor 3 (TFF3) expression is seen following gastrointestinal tract injury. In the current study, the significance of serum TFF3 as a potential diagnostic biomarker of disease activity in naїve UC patients, and its diagnostic accuracy in CRC patients were investigated. We collected serum and fecal samples from 20 cases with active UC, 20 CRC patients, and 20 normal controls. TFF3 levels were higher in patients with active UC than in controls (p < 0.001). TFF3 cut-off value of 7.9 ng/ml could predict disease activity with sensitivity and specificity of 90% and 100%, respectively. However, the combination of TFF3, C-reactive protein (CRP), and fecal calprotectin (FC) was able to predict disease activity better than each biomarker alone by raising the sensitivity and specificity to 100%. There was no correlation between TFF3, FC, and endoscopic activity in UC assessed by ulcerative colitis endoscopic index of severity (UCEIS). In the CRC patient group, the serum level of TFF3 was significantly higher when compared to controls (p=0.012). TFF3 and the degree of dysplasia were significantly correlated (r=0.496, p=0.026). At a cut-off value of 5.9 ng/ml, serum TFF3 had a diagnostic sensitivity and specificity for CRC of 82% and 90%, respectively. In conclusion, serum TFF3 may be used as a non-invasive biomarker to predict disease activity in UC both alone and in combination with CRP and FC and it could have a potential role in diagnosis of CRC.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"31 4","pages":"27-35"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of BLK and BANK1 gene polymorphisms with systemic lupus erythematous in Egyptian patients. 埃及患者 BLK 和 BANK1 基因多态性与系统性红斑狼疮的关系。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2024-10-01

Rehab A Mohammed, Sara M Elhadad, Amany M Tawfeik, Omnia A El-Dydamoni, Asmaa A Attia, Nora Seliem, Iman A Kassem, Haneya A Anani

{"title":"Association of BLK and BANK1 gene polymorphisms with systemic lupus erythematous in Egyptian patients.","authors":"Rehab A Mohammed, Sara M Elhadad, Amany M Tawfeik, Omnia A El-Dydamoni, Asmaa A Attia, Nora Seliem, Iman A Kassem, Haneya A Anani","doi":"","DOIUrl":"","url":null,"abstract":"This study examined the genotype, allelic frequencies (polymorphisms) in a sample of Egyptian patients and examined the relationship between disease activity in systemic lupus erythematosus (SLE) and the B lymphoid tyrosine kinase (BLK) and B-cell scaffold protein with ankyrin repeats 1 (BANK1) gene. This case control study involved 70 SLE patients and 40 subjects matched for age and sex as a control group. Clinical data were gathered from each participant, including SLE-related clinical activity indicators. Utilizing the restriction fragment length polymorphism (RFLP)-polymerase chain reaction (PCR), the single nucleotide polymorphisms (SNPs) BLK rs13277113G/A and BANK1 rs10516487G/A were assessed. The most prevalent genotype among the study participants was BLK rs13277113; G/G (57.1%), and BANK rs10516487; GG/ genotype (74.3%). There were no substantial variations in the incidence of genotype and allelic polymorphism between patients and controls (p>0.05). In the studied SLE patients, however, there was no significant association between both alleles (BANK rs10516487 gene alleles, G/G and G/A and BLK rs13277113, G/A, G/G and A/A) and the SLE disease activity score. While there was a significant association between BLK rs13277113 genotype alleles and age. In conclusion, BLK rs13277113 G/A and BANK1 rs10516487 G/A alleles showed no difference between SLE Egyptian patients compared to controls with no link between both genes and SLE disease activity.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"31 4","pages":"36-45"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Expression pattern of NK cells in systemic lupus erythematosus patients with different disease activities. 不同疾病活动的系统性红斑狼疮患者 NK 细胞的表达模式。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2024-10-01

Omnia M Barakat, Mohamed S Badari, Sherein G Elgendy, Muhamad R Abdel Hamed, Asmaa M Zahran, Manal M A Hassanien, Mona S Embarek

{"title":"The Expression pattern of NK cells in systemic lupus erythematosus patients with different disease activities.","authors":"Omnia M Barakat, Mohamed S Badari, Sherein G Elgendy, Muhamad R Abdel Hamed, Asmaa M Zahran, Manal M A Hassanien, Mona S Embarek","doi":"","DOIUrl":"","url":null,"abstract":"Data demonstrated the role of natural killer (NK) cells in systemic lupus erythematosus (SLE). We aimed to determine the immunophenotype and frequency of NK cells and their subsets, CXCR3, CD161 expression in blood and renal tissue of SLE patients with and without lupus nephritis and their relationship with disease activity. The study included 31 SLE patients and 11 controls. Study participants underwent full history and thorough clinical examination. SLE patients underwent routine laboratory investigations. Renal tissue biopsies were taken from patients with lupus nephritis. The frequency of NK cell subsets in blood of patients and controls and renal tissue from patients was performed by flow cytometry. An increase in circulatory CD56bright NK cells and its CD56bright CD16dim subtype was associated with the severity of systemic manifestations in SLE patients. Total CD56bright NK cells and its CD56bright CD16bright subtypes in renal tissues were related to renal damage. We detected decreased CD161 expression on NK cells related to renal damage and severity of systemic manifestations in SLE patients. Decreased expression of CXCR3 on NK cell surface in renal tissues causes misdirected trafficking of NK cells that seems to reduce the severity of lupus nephritis. In conclusion, our results paved the way to understanding the role of NK cells in the pathogenesis of SLE which may represent a future target for immune therapy of SLE. NK and CD56dim subset were decreased in the blood and increased in renal tissue of SLE patients, while the CD56bright subset was increased in blood and decreased in renal tissue reflecting their effects on renal damage and severity of manifestations in SLE.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"31 4","pages":"157-168"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of synovial repair in primary knee osteoarthritis after platelet rich plasma (PRP) intra-articular injection. 评估富血小板血浆（PRP）关节内注射后原发性膝关节骨关节炎的滑膜修复情况。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2024-10-01

Esraa M Bastawy, Mohamed G Zaki, Nevine Badr, Fatma Abdallah, Nermin H El-Gharbawy

{"title":"Assessment of synovial repair in primary knee osteoarthritis after platelet rich plasma (PRP) intra-articular injection.","authors":"Esraa M Bastawy, Mohamed G Zaki, Nevine Badr, Fatma Abdallah, Nermin H El-Gharbawy","doi":"","DOIUrl":"","url":null,"abstract":"Primary knee osteoarthritis (KOA) is a persistent condition marked by the gradual deterioration of the joint and cartilage loss on its surfaces. Recently, platelet-rich plasma (PRP) was considered a biological intervention that alleviates symptoms and restricts the advancement of primary KOA in patients. This study aimed to evaluate the effect of intra-articular PRP injections on synovial repair through cytokine assays in 20 patients with primary KOA. Patients received two intra-articular PRP injections, spaced one month apart. The role of PRP was assessed by measuring Transforming growth factor beta (TGF-β) and interleukin-17 (IL-17) levels in synovial fluid before and after the injections. Both visual analogue scale and Western Ontario and McMaster Universities Osteoarthritis index were assessed before and after intervention. IL-17 and TGF-β levels were measured in the synovial fluid using sandwich ELISA technique before the first PRP intra-articular injection and one month after the second injection to assess the synovial repair after PRP injection. Our results showed that the synovial IL-17 levels significantly decreased by 75.21% (p < 0.0001) after intra-articular knee injection, dropping from a range of 102.3-293 (median 173.5: 139.7- 224.5) to 17.86-106 (median 36.38: 23.57- 50.32). In contrast, synovial TGF-β levels significantly increased by 80.3% (p < 0.0001) after intra-articular knee injection, rising from 124-545.5 (mean ± SD: 256.22 ± 123.56) to 693.3-3226 (mean ± SD: 1521.6 ± 765.46). In conclusion, intra-articular PRP administration in primary KOA patients is associated with increased levels of TGF-β and decreased levels of IL-17 in the synovial fluid of the joint. These changes in cytokine levels suggest that PRP treatment effectively reduces inflammation and may contribute to pain relief in primary KOA.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"31 4","pages":"58-65"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of the relationship between thyroid autoimmunity, obesity and serum leptin level in a sample of Egyptian individuals. 研究埃及人样本中甲状腺自身免疫、肥胖和血清瘦素水平之间的关系。

The Egyptian journal of immunology / Egyptian Association of Immunologists Pub Date : 2024-10-01

Salma S Hassan, Salwa S Hosny, Khaled M Makboul, Ahmed M Bahaaeldin, Hanan M Ali, Nahla N Adly

{"title":"Study of the relationship between thyroid autoimmunity, obesity and serum leptin level in a sample of Egyptian individuals.","authors":"Salma S Hassan, Salwa S Hosny, Khaled M Makboul, Ahmed M Bahaaeldin, Hanan M Ali, Nahla N Adly","doi":"","DOIUrl":"","url":null,"abstract":"Obesity is a worldwide health problem, and its prevalence is increasing steadily all over the globe. Leptin, mainly produced by adipocytes, was identified to modulate the immune system, as well as contributing to increased production of thyroid peroxidase antibodies (TPO-Ab) and thyroglobulin antibody (TG-Ab). Autoimmune thyroiditis, mainly Hashimoto's thyroiditis, is believed to be the main cause of hypothyroidism in iodine sufficient regions, (TPO-Ab) and (TG-Ab) are the hallmarks of this disease. This study aimed to assess the relationship between thyroid autoimmunity, obesity and serum leptin level in a sample of Egyptian individuals. This study was a case control study which included 60 participants, recruited from the outpatient of the Internal Medicine Clinic at Ain Shams University Hospitals, during the period from February 2022 to October 2022. They were divided into two groups: Group 1 included 30 participants, have Residual hematopoiesis is an important prognostic factor of immunosuppressive therapy in severe aplastic anemia (BMI) >30 kg/m2, and Group 2 included 30 participants, have BMI (18.5-25 kg/m2). We detected serum leptin, thyroid profile and thyroid antibodies using the enzyme linked immunosorbent assay and other immunoassays. Serum levels of leptin, thyroid-stimulating hormone (TSH) and glycated hemoglobin (HbA1c) were statistically significantly higher in group 1 than in group 2 (p=0.0001 for all). Also, there was a statistically significant positive correlation between leptin level and Anti-TPO in group 1 (p=0.002). In addition, in group 1, there was a statistically significant positive correlation between serum leptin level and TSH (p=0.0001). In conclusion, there is a relationship between thyroid autoimmunity and serum leptin level in obese subjects.","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"31 4","pages":"136-144"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142477035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0