The Egyptian journal of immunology / Egyptian Association of Immunologists最新文献

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The correlation between SMAD3 and MIR 155 in Rheumatoid arthritis patients and athletes. 类风湿关节炎患者和运动员中SMAD3和MIR 155的相关性
Russul M Shuku, Angham J M Ali
{"title":"The correlation between SMAD3 and MIR 155 in Rheumatoid arthritis patients and athletes.","authors":"Russul M Shuku, Angham J M Ali","doi":"10.55133/eji.320407","DOIUrl":"https://doi.org/10.55133/eji.320407","url":null,"abstract":"<p><p>Rheumatoid arthritis (RA), an autoimmune and inflammatory condition, occurs when the immune system erroneously targets healthy cells, leading to inflammation in the affected regions. The objective of this study was to evaluate the association of Mir-155 and SMAD3 in patients with RA, athletes with RA, and control groups. The study was carried out during the beginning of January 2024 and the end of June 2024. , The study was conducted at the Medical Rehabilitation Center in AL-Sader medical city. It included 50 patients, both female and male, diagnosed with RA by a specialist physician, along with 50 individuals without the condition and 50 athletes with RA. It comprised individuals of diverse ages. We collected 5 ml of blood from patients to assess the immunological parameter SMAD3 using the Enzyme-Linked Immunosorbent Assay. Additionally, we invested MIR-155 by using the reverse transcription polymerase chain reaction (RT-PCR). There was a significant positive correlation between MIR-155 with SMAD3 (r = 0.529, p < 0.001) in the RA group, and MIR-155 has a positive correlation with SMAD3 (r = 0.574, p < 0.001) in athletes, suggesting that it may play a part in boosting signaling pathways linked to fibrosis or repair. In conclusion, there was a statistically significant positive correlation between MIR-155 and SMAD3 among RA patients and athletes compared to healthy controls.</p>","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 4","pages":"55-61"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of serum amyloid-A protein in the diagnosis of sepsis among children at PICU of Al Zahraa University Hospital. 血清淀粉样蛋白a在Al Zahraa大学医院PICU患儿脓毒症诊断中的价值
Ghadir D I A ElGharib, Soheir I Mohamed, Shimaa M K Ibrahim, Fatma Elzhraa A E Diab
{"title":"Evaluation of serum amyloid-A protein in the diagnosis of sepsis among children at PICU of Al Zahraa University Hospital.","authors":"Ghadir D I A ElGharib, Soheir I Mohamed, Shimaa M K Ibrahim, Fatma Elzhraa A E Diab","doi":"10.55133/eji.320411","DOIUrl":"https://doi.org/10.55133/eji.320411","url":null,"abstract":"<p><p>The early and efficient diagnosis of sepsis in critically ill children remains a difficult task as the clinical signs are nonspecific. Complete blood count parameters and C‑reactive protein have low sensitivity., Also, the difficulty of its diagnosis may be due to decreased positive values of blood culture and the need for longtime to detect blood culture results. The serum Amyloid A (SAA) protein level in the blood increases earlier and up to 1000‑fold in response to inflammation. This study aimed to assess the role of SAA as diagnostic and prognostic marker in pediatric sepsis in the first 24 hours after pediatric intensive care unit (PICU) admission. This case-control study included 45 children with sepsis admitted at PICU from May 2023 to March 2024 and 45 children with matched age and sex as controls. We investigated SAA level in the same time with routine laboratory investigations of both groups. SAA level was higher in the patient group, ranged from 0.9 to 47.2 µg/m, with median 4.54 µg/ml, as compared to the control group with median 0.58 µg/ml ranged from 0 to 2.3 µg/ml. (p ≤0.001). Also, SAA level was significantly lower in the survived group with median 13.6 µg/ml, ranged from 5.7 to 20 µg/ml than the non-survived group with a median of 32.3 µg/ml; ranged from 30.3 to 47.2 µg/ml. In conclusion, we found that SAA was extremely high in critical and extremely critical ill patients which can be used as a predictor of mortality in severe sepsis among children.</p>","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 4","pages":"92-100"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neutrophil CD11b and serum procalcitonin as promising markers for early detection of neonatal sepsis. 中性粒细胞CD11b和血清降钙素原作为早期检测新生儿败血症的有希望的标志物。
Shimaa A Abdel Salam, Amira A Moharram, Ayah M Shabana, Rabab Allam, Mona A Khattab, Fatma M Mahmoud
{"title":"Neutrophil CD11b and serum procalcitonin as promising markers for early detection of neonatal sepsis.","authors":"Shimaa A Abdel Salam, Amira A Moharram, Ayah M Shabana, Rabab Allam, Mona A Khattab, Fatma M Mahmoud","doi":"10.55133/eji.320403","DOIUrl":"https://doi.org/10.55133/eji.320403","url":null,"abstract":"<p><p>Although many biomarkers were used for diagnosing neonatal sepsis, none of them is conclusive alone. So, measuring multiple biomarkers were proposed to help in rapid diagnosis of neonatal sepsis. This study aimed to assess the potential role of measurement of neutrophil CD11b and serum procalcitonin (PCT) for early diagnosis of neonatal sepsis. This was a case control study, included 96 neonates admitted to Ain Shams University hospital. The neonates were divided into 3 Groups: Group A included proven sepsis Group with positive blood cultures (n=31), Group B, suspected Group with persistent clinical signs of sepsis but with negative blood cultures (n=36) and a control Group of normal newborns of matched age and sex (n=29). There was a statistically significant increase in expression of CD11b on neutrophils in Group A (median= 99.7) when compared with that of Group B (median= 99.4) and the control Group (median= 96.2) (p < 0.001), with increased mean fluorescence intensity in Group A (median= 21.3) when compared with that of Group B (median= 10.1) and control Group (median= 4.1) (p < 0.001). The receiver operating characteristic curve analysis was applied to assess the diagnostic performance of the tested markers. It showed that the serum PCT level can be used to discriminate between Group A and the control Group with 100% sensitivity, 100% specificity and CD 11b showed 100% sensitivity, 69% specificity. Moreover, to discriminate between Group A and Group B serum PCT showed 100% sensitivity, 88.9% specificity and CD 11b showed 87.1% sensitivity and 44.4% specificity. In conclusion, PCT and neutrophil CD11b are promising markers for diagnosis of early neonatal sepsis in preterm neonate.</p>","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 4","pages":"16-27"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prevalence of thyroid nodules in a sample of Egyptian systemic lupus erythematosus patients. 埃及系统性红斑狼疮患者甲状腺结节的患病率。
Hanan M Amer, Rania S Abdel Baki, Laila M A Hendawy, Mohamed R Mohamed, Islam N Abd-Elazeem, Mina M Nesim
{"title":"Prevalence of thyroid nodules in a sample of Egyptian systemic lupus erythematosus patients.","authors":"Hanan M Amer, Rania S Abdel Baki, Laila M A Hendawy, Mohamed R Mohamed, Islam N Abd-Elazeem, Mina M Nesim","doi":"10.55133/eji.320406","DOIUrl":"https://doi.org/10.55133/eji.320406","url":null,"abstract":"<p><p>Thyroid nodules (TNs) are distinct lesions inside the thyroid that can be determined from the adjacent thyroid parenchyma on ultrasonography. Complement activation, autoantibody generation, persistent inflammation, and immune-complex deposition are the hallmarks of systemic lupus erythematosus (SLE), a complicated systemic autoimmune condition that damages tissues and organs. The current study aimed to detect TNs prevalence in SLE patients, and possible malignancy in a sample of Egyptian population. This cross-sectional study included 80 cases diagnosed with systemic lupus erythematosus, attending the Endocrinology and Rheumatology outpatient clinics at Ain-Shams University Hospital, to detect the prevalence of TNs in a sample of Egyptian SLE cases. There was a positive relation between Systemic Lupus Erythematosus Disease Activity Index (SELDAI) score and TNs, also positive relation between SELDAI score and prevalence of malignancy. There was a higher remarkable number, 13 patients (43.3%), of TNs cases among the group with abnormal thyroid functions. In conclusion, the percent of thyroid malignancy among patients with SLE is the same as in general population, so that SLE itself did not increase the risk of thyroid malignancy. Also, the percent of TNs among cases with SLE was the same as in the general population. Activity of SLE in terms of SLEDAI score has relation with TNs and malignancy. Cases with SLE and TNs are more susceptible to thyroid dysfunctions.</p>","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 4","pages":"45-54"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of high-mobility group box 1 in late onset neonatal sepsis. 高流动性组盒1在迟发性新生儿脓毒症中的作用。
Hanan H Zeiada, Safaa A ELMeneza, Iman M El-Bagoury
{"title":"Role of high-mobility group box 1 in late onset neonatal sepsis.","authors":"Hanan H Zeiada, Safaa A ELMeneza, Iman M El-Bagoury","doi":"10.55133/eji.320413","DOIUrl":"https://doi.org/10.55133/eji.320413","url":null,"abstract":"<p><p>Neonatal sepsis is an important cause of morbidity and mortality. High mobility group box1 protein (HMGB1) is a cytokine that can mediate inflammation. The aim of this research was to investigate the role of HMGB1 in diagnosis and prognosis of late onset neonatal sepsis. This observational case-control study included 80 newborn infants ≥37 weeks of gestation. Newborn infants were assigned into two groups: the late-onset neonatal septic group included 40 infant cases, and the control group included 40 newborn infants. Clinical sepsis score, hematological sepsis score and serum level of C-reactive protein were assessed and blood culture performed. HMGB1 was measured by an enzyme-linked immunosorbent assay. There was a significant increase of HMGB1 in the late-onset neonatal septic group than the control group (p < 0. 001). The best cut off point of HMGB 1 to discriminate against the late-onset sepsis cases from the control newborn infants was > 68 ng/ml with a sensitivity of 97.5%, specificity of 95%, positive predictive value of 95.1% and negative predictive value of 97.4% with total accuracy of 0.99%. The values of HMGB1 were not affected by gestational age, birth weight, postnatal age or gender. There were no significant differences in mean values between survival and non-survival cases. The best cut off value to predict mortality in the late onset sepsis group was >167.8 ng/ml with a sensitivity of 60% and specificity of 54.29%. In conclusion, this study suggested that HMGB1 is a promising marker for diagnosis of late onset neonatal sepsis in full term infants, on the contrary HMGB1 could not predict mortality in neonatal septic patients.</p>","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 4","pages":"110-115"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A study of circulating levels of IL-17 in Egyptian patients with Behcet's disease and its association with disease manifestation. 埃及白塞病患者血液中IL-17水平的研究及其与疾病表现的关系
Dina H M Abdella, Nema A Soliman, Alaa M El-Salawy, Shaimaa A Abdelwahab
{"title":"A study of circulating levels of IL-17 in Egyptian patients with Behcet's disease and its association with disease manifestation.","authors":"Dina H M Abdella, Nema A Soliman, Alaa M El-Salawy, Shaimaa A Abdelwahab","doi":"10.55133/eji.320404","DOIUrl":"https://doi.org/10.55133/eji.320404","url":null,"abstract":"<p><p>Behcet's disease (BD) is a vascular inflammatory illness with different clinical presentations and incompletely understood mechanisms. Interleukin-17 (IL-17) is suggested to take part in BD inflammatory process but the exact role in different disease manifestation is still unclear. This case- control study aimed to investigate the correlation between IL-17 levels and the symptoms of BD. This study included 30 individuals diagnosed with BD and 15 apparently healthy volunteers, age and sex matched, as controls. Complete clinical assessment with consideration for clinical manifestations was performed. Laboratory markers for BD activity including erythrocyte sedimentation rates and C-reactive protein (CRP) were evaluated. Serum IL-17 level was measured using an enzyme-linked immunosorbent assay. The serum IL-17 levels in patients and controls differed significantly (p < 0.001). Both the duration of BD illness and CRP showed a significant positive correlation with serum IL-17 (p= 0.048 and p < 0.001, respectively). Furthermore, compared to patients with uveitis and controls, individuals with skin manifestations had significantly greater serum IL-17 levels (p < 0.001). In conclusion, serum IL-17 in BD patients especially in those with skin manifestations had significant correlation with disease activity. These results confirm the suggested role of IL-17 in augmenting the inflammation of BD and support the treatment with anti-IL-17 specific therapy for resistant cases of BD especially cases with skin symptoms.</p>","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 4","pages":"28-34"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of human leucocyte antigen (DQ) in acute renal allograft rejection: A single center study. 人白细胞抗原(DQ)在急性肾移植排斥反应中的作用:一项单中心研究。
Tarek T H ElMelegy, Sohair K Sayed, Maha G Abd El-Kadrd, Abeer A Mokhtar
{"title":"Role of human leucocyte antigen (DQ) in acute renal allograft rejection: A single center study.","authors":"Tarek T H ElMelegy, Sohair K Sayed, Maha G Abd El-Kadrd, Abeer A Mokhtar","doi":"10.55133/eji.320409","DOIUrl":"https://doi.org/10.55133/eji.320409","url":null,"abstract":"<p><p>Human leukocyte antigen (HLA) system is a very polymorphic gene complex encoding for cell surface proteins. Kidney transplantation (KT) is considered the optimal renal replacement therapy. Donor-specific antibodies (DSA) against HLA class II antigens are more common than class I. This study aimed to determine the role of HLA (DQ) in acute rejection of renal allograft. This study included 43 KT recipient donor pairs. HLA typing (A, B, DR) of donor and recipient and flowcytometry cross matching results pre transplantation were collected from patients' files. Panel Reactive Antibody (PRA) classes I and II were done to recipients pre transplantation. PRA classes I and II and HLA-DQ genotyping were done to recipients 15-16 weeks post-transplantation. Rejection occurred in 9.3% recipients. Recipients with positive PRA class II had a statistically significant higher percentage of rejection (25.0%) compared to (0.0%) of those with negative PRA class II (p=0.029). Recipients with positive anti HLA-DR antibodies (Abs) and anti HLA-DQ Abs had statistically significant higher percent of rejection (28.6%) compared to (0.0%) of those with negative anti HLA-DR Abs and anti HLA-DQ Abs (p= 0.016). Recipients with positive anti HLA-DQ4 Abs or anti HLA-DQ5 Abs had a statistically significant higher percent of rejection (50%) compared to (5.1%) of those without anti HLA-DQ4 Abs or anti HLA-DQ5 Abs (p=0.037). Recipients with positive anti HLA-DQ9 Abs had a statistically significant higher percent of rejection (30%) compared to (3.0%) of those without anti HLA-DQ9 Abs (p=0.034). Recipients who received kidney from HLA-DQ mismatched donors had higher incidence (57%) of anti HLA-DQ5 antibodies and anti HLA-DQ6 antibodies compared to those with HLA-DQ matched donors (0.0%) (p= 0.018). In conclusion, anti HLA-DQ antibody was one of the most prevalent post-transplant PRA detected. Regarding acute rejection, there was no risk association between its occurrence and HLA-DQ mismatching.</p>","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 4","pages":"69-83"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of IL-17 in multiple sclerosis and its relationship with disease severity in Egypt. IL-17在埃及多发性硬化症中的评估及其与疾病严重程度的关系
Mona Salama, Yara Mareia, Fadia Attia, Maha Anani, Mohamed El-Samahy, Shaimaa Mohamed, Sameer Alqassimi, Eman S Albeltagy, Eman Roshdey, Sahar Ahmed, Mohamed Greash
{"title":"Assessment of IL-17 in multiple sclerosis and its relationship with disease severity in Egypt.","authors":"Mona Salama, Yara Mareia, Fadia Attia, Maha Anani, Mohamed El-Samahy, Shaimaa Mohamed, Sameer Alqassimi, Eman S Albeltagy, Eman Roshdey, Sahar Ahmed, Mohamed Greash","doi":"10.55133/eji.320402","DOIUrl":"https://doi.org/10.55133/eji.320402","url":null,"abstract":"<p><p>Central nervous system (CNS) demyelination and neurodegeneration are hallmarks of multiple sclerosis (MS). The pathophysiology of MS is related to the inflammatory cytokine interleukin-17 (IL-17), and there may be an association between IL-17 levels and disease severity. In order to find a non-invasive biomarker for disease progression, serum IL-17 levels in MS patients were measured in comparison to a matched control group. Additionally, the relationship between serum IL-17 levels and MS activity was examined. This case-control study included 31 MS patients who were diagnosed using the 2017 updated McDonald's criteria and a control group consisted of 20 apparently healthy individuals, matched for both age and sex. Serum samples were collected and analyzed for IL-17 concentrations using ELISA commercial kits. Clinical evaluations included demographic data, medical history, and an assessment of disease severity using the Expanded Disability Status Scale (EDSS). The study assessed serum levels of IL-17, and found significantly higher levels in MS patients (38.79± 37.36) in comparison to controls (1.47± 0.49). Levels of IL-17 were increased in secondary progressive MS patients than in relapsing-remitting MS patients, but did not reach statistical significance. No significant associations were identified between IL-17 levels and the duration of the MS disease or the frequency of relapses. In conclusion, the study pointed to increased IL-17 levels as a potential MS biomarker.</p>","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 4","pages":"8-15"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The comparative immunophenotypic analysis of paediatric and adults bone marrow lymphocytes' in chronic Immune thrombocytopenic purpura (ITP) refractory to intravenous immunoglobulin and corticosteroids. 慢性免疫性血小板减少性紫癜(ITP)患儿与成人骨髓淋巴细胞免疫表型比较分析
Nagwa Hassanein, Kehinde O Oloko, Bargavi Balakrishnan, Iman A Kassem, Fawzia A Sharaf
{"title":"The comparative immunophenotypic analysis of paediatric and adults bone marrow lymphocytes' in chronic Immune thrombocytopenic purpura (ITP) refractory to intravenous immunoglobulin and corticosteroids.","authors":"Nagwa Hassanein, Kehinde O Oloko, Bargavi Balakrishnan, Iman A Kassem, Fawzia A Sharaf","doi":"10.55133/eji.320410","DOIUrl":"https://doi.org/10.55133/eji.320410","url":null,"abstract":"<p><p>Immune thrombocytopenic purpura (ITP) is an acquired autoimmune disorder marked by increased platelet destruction and reduced production, primarily due to the presence of autoantibodies. While many patients respond well to therapy, a subset remains refractory, posing a significant challenge for clinicians and patients alike. This study aimed to evaluate the bone marrow lymphocyte composition in both pediatric and adult patients with refractory ITP, with the goal of uncovering possible reasons for treatment resistance. This case-control study was conducted during the period from May 2019 to December 2024, involved 30 children and 37 adults diagnosed with refractory ITP, along with 60 age- and sex-matched normal controls. All participants underwent bone marrow aspiration, followed by immunophenotyping using a standard panel. Children with refractory ITP exhibited significantly higher percentages of hematogones and mature B cells in the bone marrow (8.27% ± 4.50% and 4.52% ± 3.82%, respectively) compared to adults (1.17% ± 2.18% and 1.89% ± 1.33%). In contrast, the adult group showed elevated levels of T cells (CD3+), averaging 11.6% ± 6.25% compared to 7.80% ± 3.12% in the pediatric group (p = 0.014). Additionally, both CD4+ and CD8+ T cells were significantly more abundant in adults (6.62% ± 4.82% and 7.89% ± 7.02%). Significant differences were also observed between patient and control groups in the proportions of CD3+, CD4+, CD4/CD8 ratios, hematogones, and mature B cells. In conclusion, distinct immunophenotypic profiles in bone marrow lymphocytes were identified between pediatric and adult patients with refractory ITP. These findings highlight potential age-related differences in disease mechanisms, which could inform more targeted approaches to diagnosis and management.</p>","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 4","pages":"84-91"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Is there a role of miRNA -330-3p and miRNA-362-3p in Lupus Nephritis? miRNA -330-3p和miRNA-362-3p在狼疮性肾炎中是否有作用?
Mohamed F A Assar, Eman A Badr, Safwa O Toulan, Ashwak N I Elhalafawy, Basma A Mostafa
{"title":"Is there a role of miRNA -330-3p and miRNA-362-3p in Lupus Nephritis?","authors":"Mohamed F A Assar, Eman A Badr, Safwa O Toulan, Ashwak N I Elhalafawy, Basma A Mostafa","doi":"10.55133/eji.320405","DOIUrl":"https://doi.org/10.55133/eji.320405","url":null,"abstract":"<p><p>Systemic lupus erythematosus (SLE) is an autoimmune disease, with lupus nephritis (LN) being one of its most serious complications. MicroRNAs, particularly miRNA-330-3p and miRNA-362-3p, were implicated in immune regulation and inflammation. This study aimed to evaluate the potential role of miRNA-330-3p and miRNA-362-3p in the progression of SLE and LN. This cross-sectional study included 150 participants: 50 controls (Group I), 50 SLE patients without nephritis (Group II), and 50 patients with lupus nephritis (Group III). Serum levels of miRNA-330-3p and miRNA-362-3p were quantified using a real-time polymerase chain reaction (PCR) test. Clinical and laboratory parameters were assessed, including disease activity and nephritis classification. miRNA-330-3p levels were significantly lower in both patients without nephritis (1.119 ± 1.289) and patients with nephritis (0.89 ± 0.518) compared to controls (1.312 ± 0.480; p < 0.001). miRNA-362-3p levels were significantly lower in patients with nephritis (0.623 ± 0.925) than in both controls (1.268 ± 0.419; p < 0.001) and patients without nephritis (1.254 ± 1.351; p < 0.001). The receiver operating characteristic (ROC) curve analysis revealed that miRNA-362-3p discriminated LN from non-LN SLE patients (AUC = 0.754; cut off ≤ 0.204; sensitivity 66%, specificity 72%). Both miRNA-330-3p and miRNA-362-3p are down regulated in SLE, particularly in patients with LN. These miRNAs may represent therapeutic targets, pending validation in future studies.</p>","PeriodicalId":39724,"journal":{"name":"The Egyptian journal of immunology / Egyptian Association of Immunologists","volume":"32 4","pages":"35-44"},"PeriodicalIF":0.0,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145287189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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