Nanomedicine: Nanotechnology, Biology and Medicine最新文献

{"title":"Cell membrane-camouflaged liposomes and neopeptide-loaded liposomes with TLR agonist R848 provides a prime and boost strategy for efficient personalized cancer vaccine therapy","authors":"Lu Shi PhD,&nbsp;Hongchen Gu PhD","doi":"10.1016/j.nano.2022.102648","DOIUrl":"https://doi.org/10.1016/j.nano.2022.102648","url":null,"abstract":"<div><p><span>Recent advances in bioinformatics and nanotechnology offer great opportunities for personalized cancer vaccine<span> development. However, the timely identification of neoantigens<span><span><span> and unsatisfactory efficacy of therapeutic cancer vaccines remain two obstacles for clinical transformation. We propose a “prime and boost” strategy to facilitate neoantigen-based immunotherapy. To prime the immune system, we first constructed personalized </span>liposomes<span> with cancer cell membranes and adjuvant </span></span>R848<span> to provide immunostimulatory efficacy and time for identifying tumor antigens. Liposomes loaded with personalized neopeptides and adjuvants were used to boost the immune response. </span></span></span></span><em>In vitro</em><span> experiments verified potent immune responses, including macrophage polarization<span>, dendritic cell maturation, and T lymphocyte activation. </span></span><em>In vivo</em> B16F10 and TC-1 cancer model were used to investigate efficient tumor growth suppression. Liposomal vaccines with neopeptides could stimulate human dendritic cells and T lymphocytes <em>in vitro</em>. These results demonstrate that the “prime and boost” strategy provides simple, quick, and efficient personalized vaccines for cancer therapy.</p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"48 ","pages":"Article 102648"},"PeriodicalIF":5.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1567237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted poly(L-glutamic acid)-based hybrid peptosomes co-loaded with doxorubicin and USPIONs as a theranostic platform for metastatic breast cancer","authors":"Maliheh Hasannia PhD ,&nbsp;Kamran Lamei PhD ,&nbsp;Khalil Abnous PhD ,&nbsp;Seyed Mohammad Taghdisi PhD ,&nbsp;Sirous Nekooei MD, PhD ,&nbsp;Negar Nekooei MD ,&nbsp;Mohammad Ramezani PhD ,&nbsp;Mona Alibolandi PhD","doi":"10.1016/j.nano.2022.102645","DOIUrl":"https://doi.org/10.1016/j.nano.2022.102645","url":null,"abstract":"<div><p><span><span><span>Peptosomes, as a vesicular polypeptide-based system and a versatile carrier for co-delivery of hydrophilic<span> and hydrophobic materials, provide great delivery opportunities due to the intrinsic biocompatibility<span><span> and biodegradability of the polypeptides </span>backbone. In the current study, a novel poly(L-glutamic acid)-block-polylactic acid di-block </span></span></span>copolymer (PGA-PLA) was synthesized in two steps. Firstly, γ-benzyl L-glutamate-N-carboxy </span>anhydride<span><span><span> (BLG-NCA) and 3,6-dimethyl-1,4-dioxane-2,5-dione were polymerized using N-hexylamine and benzyl alcohol<span> as initiators to produce poly(γ-benzyl L-glutamate (PBLG) and polylactic acid. Then, PBLG was deprotected to produce PGA. Secondly, PGA was conjugated to the benzyl-PLGA to fabricate PGA-PLA diblock copolymer. The synthesized diblock copolymer was used for the encapsulation of </span></span>doxorubicin, as hydrophilic anticancer and ultra-small superparamagnetic iron oxide </span>nanoparticles<span> (USPIONs) as hydrophobic contrast agent within aqueous core and bilayer of vesicular peptosome, respectively </span></span></span><em>via</em><span><span> double emulsion method. The prepared peptosomes (Pep@USPIONs-DOX) controlled the release of DOX (&lt;15 % of the encapsulated DOX release up to 240 h of incubation at the physiological conditions) while increasing the stability and solubility of the hydrophobic USPIONs. Then, AS1411 </span>DNA<span><span><span> aptamer was decorated on the surface of the PGA-PLA peptosomes (Apt-Pep@USPIONs-DOX). The prepared targeted and non-targeted platforms showed spherical morphology with </span>hydrodynamic sizes of 265 ± 52 and 229 ± 44 </span>nm respectively. </span></span><em>In vitro</em><span> cellular cytotoxicity<span><span> and cellular uptake were studied in nucleolin<span> positive (4T1) and nucleolin negative (CHO) cell lines. Cellular uptake of the targeted formulation was greater than that of non-targeted peptosome, while cellular internalization of these peptosomes was identical in </span></span>CHO<span> cells. Moreover, targeted peptosomes showed greater toxicity than non-targeted peptosome in 4T1 cell line<span>. The prepared theranostic targeted peptosomes demonstrated improved capability in terms of survival rate, biodistribution, tumor suppression efficiency, and MR imaging in the 4T1 tumor-bearing mice.</span></span></span></span></p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"48 ","pages":"Article 102645"},"PeriodicalIF":5.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3342721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid nanocapsules for the nose-to-brain delivery of the anti-inflammatory bioactive lipid PGD2-G","authors":"Ariane Mwema M BBMC ,&nbsp;Pauline Bottemanne PhD ,&nbsp;Adrien Paquot M FARM ,&nbsp;Bernard Ucakar Bachelor ,&nbsp;Kevin Vanvarenberg Bachelor ,&nbsp;Mireille Alhouayek PhD ,&nbsp;Giulio G. Muccioli PhD ,&nbsp;Anne des Rieux PhD","doi":"10.1016/j.nano.2022.102633","DOIUrl":"https://doi.org/10.1016/j.nano.2022.102633","url":null,"abstract":"<div><p>Here, prostaglandin D₂-glycerol ester (PGD₂-G) was selected to target neuroinflammation. As PGD₂-G is reported to have a short plasmatic half-life, we propose to use lipid nanocapsules (LNC) as vehicle to safely transport PGD₂-G to the central nervous system (CNS). PGD₂-G-loaded LNC (PGD₂-G-LNC) reduced pro-inflammatory cytokine expression in activated microglial cells, even so after crossing a primary olfactory cell monolayer. A single nasal administration of PGD₂-G-LNC in lipopolysaccharide (LPS)-treated mice reduced pro-inflammatory cytokine expression in the olfactory bulb. Coating LNC's surface with a cell-penetrating peptide, transactivator of transcription (TAT), increased its accumulation in the brain. Although TAT-coated PGD₂-G-LNC modestly exerted its anti-inflammatory effect in a mouse model of multiple sclerosis similar to free PGD₂-G after nasal administration, TAT-coated LNC surprisingly reduced the expression of pro-inflammatory chemokines in the CNS. These data propose LNC as an interesting drug delivery tool and TAT-coated PGD₂-G-LNC remains a good candidate, in need of further work.</p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"48 ","pages":"Article 102633"},"PeriodicalIF":5.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3457442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-guided chemoradiotherapy of breast cancer using smart methotrexate-loaded perfluorohexane nanodroplets","authors":"Roghayeh Kamran Samani PhD ,&nbsp;Fatemeh Maghsoudinia PhD ,&nbsp;Fatemeh Mehradnia PhD ,&nbsp;Seyed Hossein Hejazi PhD ,&nbsp;Mohsen Saeb PhD ,&nbsp;Tayebe Sobhani MSc ,&nbsp;Zohreh Farahbakhsh MSc ,&nbsp;Masoud A. Mehrgardi PhD ,&nbsp;Mohamad Bagher Tavakoli PhD","doi":"10.1016/j.nano.2022.102643","DOIUrl":"https://doi.org/10.1016/j.nano.2022.102643","url":null,"abstract":"<div><p><span><span>Chemoradiotherapy with controlled-release </span>nanocarriers<span><span> such as sono-sensitive nanodroplets (NDs) can enhance the anticancer activity of chemotherapy medicines and reduces normal tissue side effects. In this study, folic acid-functionalized methotrexate-loaded </span>perfluorohexane<span> NDs with alginate shell (FA-MTX/PFH@alginate NDs) were synthesized, characterized, and their potential for ultrasound-guided chemoradiotherapy of breast cancer was investigated </span></span></span><em>in vitro</em> and <em>in vivo</em><span><span>. The cancer cell (4T1) viabilities and surviving fractions after NDs and ultrasound </span>treatments were significantly decreased. However, this reduction was much more significant for ultrasound in combination with X-ray irradiation. The </span><em>in vitro</em> and <em>in vivo</em><span><span> results confirmed that MTX-loaded NDs are highly biocompatible and they have no significant hemolytic activity and </span>organ toxicity. Furthermore, the </span><em>in vivo</em><span> results indicated that the FA-MTX/PFH@alginate NDs were accumulated selectively in the tumor region. In conclusion, FA-functionalized MTX/PFH@alginate NDs have a great theranostic performance for ultrasound-controlled drug delivery in combination with radiotherapy of breast cancer.</span></p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"48 ","pages":"Article 102643"},"PeriodicalIF":5.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3457443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spotlight on mycobacterial lipid exploitation using nanotechnology for diagnosis, vaccines, and treatments","authors":"Carlos M. Valdemar-Aguilar MSc ,&nbsp;Ravichandran Manisekaran PhD ,&nbsp;Laura S. Acosta-Torres PhD ,&nbsp;Luz M. López-Marín PhD","doi":"10.1016/j.nano.2023.102653","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102653","url":null,"abstract":"<div><p>Tuberculosis (TB), historically the most significant cause of human morbidity and mortality, has returned as the top infectious disease worldwide, under circumstances worsened by the COVID-19 pandemic's devastating effects on public health. Although <em>Mycobacterium tuberculosis</em>, the causal agent, has been known of for more than a century, the development of tools to control it has been largely neglected. With the advancement of nanotechnology, the possibility of engineering tools at the nanoscale creates unique opportunities to exploit any molecular type. However, little attention has been paid to one of the major attributes of the pathogen, represented by the atypical coat and its abundant lipids. In this review, an overview of the lipids encountered in <em>M. tuberculosis</em> and interest in exploiting them for the development of TB control tools are presented. Then, the amalgamation of nanotechnology with mycobacterial lipids from both reported and future works are discussed.</p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"48 ","pages":"Article 102653"},"PeriodicalIF":5.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9839462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3021359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dye labeling for optical imaging biases drug carriers' biodistribution and tumor uptake","authors":"Sarah Schraven MSc ,&nbsp;Stefanie Rosenhain PhD ,&nbsp;Ramona Brueck ,&nbsp;Tim Marvin Wiechmann MSc ,&nbsp;Robert Pola PhD ,&nbsp;Tomáš Etrych PhD ,&nbsp;Wiltrud Lederle DSc ,&nbsp;Twan Lammers PhD ,&nbsp;Felix Gremse PhD ,&nbsp;Fabian Kiessling MD","doi":"10.1016/j.nano.2023.102650","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102650","url":null,"abstract":"<div><p><span><span>Biodistribution analyses of </span>nanocarriers<span><span> are often performed with optical imaging. Though dye tags can interact with transporters, e.g., organic anion transporting </span>polypeptides<span> (OATPs), their influence on biodistribution was hardly studied. Therefore, this study compared tumor cell uptake and biodistribution (in A431 tumor-bearing mice) of four near-infrared fluorescent dyes (AF750, IRDye750, Cy7, DY-750) and dye-labeled poly(</span></span></span><em>N</em><span><span>-(2-hydroxypropyl)methacrylamide)-based nanocarriers (dye-pHPMAs). Tumor cell uptake of hydrophobic dyes (Cy7, DY-750) was higher than that of hydrophilic dyes (AF750, IRDye750), and was actively mediated but not related to OATPs. Free dyes' elimination depended on their </span>hydrophobicity<span>, and tumor uptake correlated with blood circulation times. Dye-pHPMAs circulated longer and accumulated stronger in tumors than free dyes. Dye labeling significantly influenced nanocarriers' tumor accumulation and biodistribution. Therefore, low-interference dyes and further exploration of dye tags are required to achieve the most unbiased results possible. In our assessment, AF750 and IRDye750 best qualified for labeling hydrophilic nanocarriers.</span></span></p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"48 ","pages":"Article 102650"},"PeriodicalIF":5.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"2891282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinspired low-density lipoprotein co-delivery system for targeting and synergistic cancer therapy","authors":"Qiaoling Zhu MD ,&nbsp;Yajie Wang MD ,&nbsp;Ying Huang MD ,&nbsp;Yixuan Zi MD ,&nbsp;Simin Yan PhD ,&nbsp;Xianbiao Shi PhD ,&nbsp;Jun Cai MD ,&nbsp;Haixia Zhang MD ,&nbsp;Jianfeng Sang PhD ,&nbsp;Wenli Zhang PhD ,&nbsp;Weihong Ge MD","doi":"10.1016/j.nano.2022.102641","DOIUrl":"https://doi.org/10.1016/j.nano.2022.102641","url":null,"abstract":"<div><p>Epithelial-mesenchymal transition (EMT) is the culprit of tumor invasion and metastasis. As a critical transcription factor that induces EMT, snail is of great importance in tumor progression, and knocking down its expression by small interfering RNA (siRNA) may inhibit tumor metastasis. Herein, we developed a core-shelled bioinspired low-density lipoprotein (bio-LDL) in which snail siRNA-loaded calcium phosphate nanoparticles were wrapped as the core and doxorubicin was embedded in the outer phospholipids modified with a synthetic peptide of apoB100 targeting LDL receptor-abundant tumor cells. Bio-LDL exhibited pH-responsive release, lysosomal escape ability, enhanced cytotoxicity and apoptotic induction. Bio-LDL could significantly inhibit the expression of snail and regulate EMT-related proteins to reduce tumor migration and invasion <em>in vitro</em>. Bio-LDL also displayed favorable tumor targeting and synergistic inhibition of tumor growth and metastasis <em>in vivo</em>. Therefore, the multifunctional bio-LDL will be a promising co-delivery vector and holds potential value for clinical translation.</p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"48 ","pages":"Article 102641"},"PeriodicalIF":5.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1567235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between human colon cancer specific antigens and Raman spectra. Attempting to use Raman spectroscopy in the determination of tumor markers for colon cancer","authors":"Joanna Depciuch PhD ,&nbsp;Paweł Jakubczyk Prof. ,&nbsp;Wiesław Paja PhD ,&nbsp;Krzysztof Pancerz PhD ,&nbsp;Agnieszka Wosiak Prof. ,&nbsp;Monika Kula-Maximenko PhD ,&nbsp;İlhan Yaylım Prof. ,&nbsp;Güldal İnal Gültekin Prof. ,&nbsp;Nevzat Tarhan Prof. ,&nbsp;Mehmet Tolgahan Hakan PhD ,&nbsp;Dilara Sönmez M.Sc ,&nbsp;Devrim Sarıbal Prof. ,&nbsp;Soykan Arıkan Prof. ,&nbsp;Zozan Guleken Prof.","doi":"10.1016/j.nano.2023.102657","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102657","url":null,"abstract":"<div><p>Colorectal cancer is the second most common cause of cancer-related deaths worldwide. To follow up on the progression of the disease, tumor markers are commonly used. Here, we report serum analysis based on Raman spectroscopy to provide a rapid cancer diagnosis with tumor markers and two new cell adhesion molecules measured using the ELİSA method. Raman spectra showed higher Raman intensities at 1447 cm⁻¹ 1560 cm⁻¹, 1665 cm^-1, and 1769 cm⁻¹, which originated from CH₂ proteins and lipids, amide II and amide I, and C<img>O lipids vibrations. Furthermore, the correlation test showed, that only the CEA colon cancer marker correlated with the Raman spectra. Importantly, machine learning methods showed, that the accuracy of the Raman method in the detection of colon cancer was around 95 %. Obtained results suggest, that Raman shifts at 1302 cm⁻¹ and 1306 cm⁻¹ can be used as spectroscopy markers of colon cancer.</p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"48 ","pages":"Article 102657"},"PeriodicalIF":5.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"2377483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic evaluation of the effect of different apolipoprotein A-I mimetic peptides on the performance of synthetic high-density lipoproteins in vitro and in vivo","authors":"Wenmin Yuan Ph.D. ,&nbsp;Kelsey Ernst PharmD ,&nbsp;Rui Kuai Ph.D. ,&nbsp;Emily E. Morin Ph.D. ,&nbsp;Minzhi Yu Ph.D. ,&nbsp;Denis O. Sviridov Ph.D. ,&nbsp;Jie Tang Ph.D. ,&nbsp;Ling Mei Ph.D. ,&nbsp;Dan Li Ph.D. ,&nbsp;Rose Ackermann MS ,&nbsp;Alan T. Remaley M.D., Ph.D. ,&nbsp;Anna Schwendeman Ph.D.","doi":"10.1016/j.nano.2022.102646","DOIUrl":"https://doi.org/10.1016/j.nano.2022.102646","url":null,"abstract":"<div><p><span><span>Synthetic high-density lipoproteins nanomedicine<span> (sHDL) composed of apolipoprotein A-I (ApoA-I) </span></span>mimetic peptides<span><span> and lipids have shown very promising results for the </span>treatment<span> of various cardiovascular diseases. Numerous efforts have also been made to design different ApoA-I mimetic peptides to improve the potency of sHDL, especially the efficiency of reverse cholesterol transport<span>. However, the way in which ApoA-I mimetic peptides affect the properties of sHDL, including stability, cholesterol efflux, cholesterol esterification, elimination </span></span></span></span><em>in vivo</em><span><span>, and the relationship of these properties, is still poorly understood. Revealing the effect of these factors on the potency of sHDL is important for the design of better ApoA-I mimetic peptides. In this study, three widely used ApoA-I mimetic peptides with different sequences, lengths, LCAT activation and lipid </span>binding affinities were used for the preparation of sHDL and were evaluated in terms of physical/chemical properties, cholesterol efflux, cholesterol esterification, remodeling, and pharmacokinetics/pharmacodynamics. Our results showed that ApoA-I mimetic peptides with the highest cholesterol efflux and cholesterol esterification </span><em>in vitro</em> did not exhibit the highest cholesterol mobilization <em>in vivo</em><span>. Further analysis indicated that other factors, such as pharmacokinetics and remodeling of sHDL, need to be considered in order to predict the efficiency of cholesterol mobilization </span><em>in vivo</em>. Thus, our study highlights the importance of using the overall performance, rather than <em>in vitro</em> results alone, as the blueprint for the design and optimization of ApoA-I mimetic peptides.</p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"48 ","pages":"Article 102646"},"PeriodicalIF":5.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3210035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutrophil membrane-coated nanoparticles exhibit increased antimicrobial activities in an anti-microbial resistant K. pneumonia infection model","authors":"Jun Liu MM ,&nbsp;Xiaochun Chen BS ,&nbsp;Lei Xu MM ,&nbsp;Fan Tu BS ,&nbsp;Xiaohong Rui BS ,&nbsp;Lizhu Zhang MM ,&nbsp;Zhihan Yan BS ,&nbsp;Yun Liu MM ,&nbsp;Renjing Hu BS","doi":"10.1016/j.nano.2022.102640","DOIUrl":"https://doi.org/10.1016/j.nano.2022.102640","url":null,"abstract":"<div><h3>Objective</h3><p><span>To investigate the efficacy and safety of neutrophil<span><span><span> membrane-coated nanoparticles mediated KLA peptides (KLAKLAKKLAKLAK) and </span>gentamicin in the </span>targeted therapy of anti-microbial resistant </span></span><span><em>Klebsiella </em><em>pneumoniae</em></span> (<em>K. pneumonia</em>) lung infection.</p></div><div><h3>Methods</h3><p>The characteristics of KLA-neutrophils nanoparticles (NNPs) are identified <em>via</em><span><span><span> dynamic light scattering (DLS), transmission </span>electron microscope<span> (TEM), SDS-PAGE, Western blot, quantitative flow cytometry (QFCM) and </span></span>confocal microscopy. The safety of KLA-NNPs both </span><em>in vitro</em> and <em>in vivo</em><span><span><span> is evaluated by hemolysis test, platelet α granule </span>membrane protein concentration, protein </span>adsorption capacity, </span><em>in vitro</em><span> macrophage phagocytosis<span><span>, weight change<span>, liver function indicators, blood biochemical indicators, and pathological changes of vital organs in mice. The efficacy of KLA-NNPs is determined by time-kill assay, </span></span>fluorescent label test, intracellular bacterial content, caspase-1 activity, survival rate, and HE staining both </span></span><em>in vitro</em> and <em>in vivo</em>.</p></div><div><h3>Results</h3><p><span>The prepared KLA-NNPs have a typical “core-shell” structure, uniform nanometer size, and retain the membrane proteins on the neutrophil membrane that achieve functional effects. </span><em>In vitro</em> safety analysis showed that KLA-NNPs have good blood compatibility and can inhibit macrophage phagocytosis <em>in vitro</em><span>. KLA-NNPs can effectively release KLA and significantly reduce intracellular bacteria and caspase-1 activity. </span><em>In vivo</em><span> safety analysis and efficacy analysis revealed that KLA-NNPs have good biocompatibility and could effectively improve the survival rate of mice.</span></p></div><div><h3>Conclusion</h3><p><span>The prepared KLA-NNPs have good nano-medicine chemical and physical properties and safety. It can evade immune system clearance, achieve high-efficiency targeted aggregation and drug delivery to bacterial infection sites, and effectively inhibit the development of pneumonia induced by drug-resistant </span><em>K. pneumonia</em>.</p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"48 ","pages":"Article 102640"},"PeriodicalIF":5.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3457445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}