Nanomedicine: Nanotechnology, Biology and Medicine最新文献_第5页

Evaluation of cannabidiol nanoparticles and nanoemulsion biodistribution in the central nervous system after intrathecal administration for the treatment of pain 评估大麻二酚纳米颗粒和纳米乳在鞘内给药治疗疼痛后中枢神经系统的生物分布

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-04-01 DOI: 10.1016/j.nano.2023.102664

Paula Muresan PhD , Stephen Woodhams PhD , Fiona Smith MPharm , Vincenzo Taresco PhD , Jaymin Shah PhD , Mei Wong PhD , Victoria Chapman PhD , Stuart Smith MD, PhD , Gareth Hathway PhD , Ruman Rahman PhD , Pavel Gershkovich PhD , Maria Marlow PhD

{"title":"Evaluation of cannabidiol nanoparticles and nanoemulsion biodistribution in the central nervous system after intrathecal administration for the treatment of pain","authors":"Paula Muresan PhD , Stephen Woodhams PhD , Fiona Smith MPharm , Vincenzo Taresco PhD , Jaymin Shah PhD , Mei Wong PhD , Victoria Chapman PhD , Stuart Smith MD, PhD , Gareth Hathway PhD , Ruman Rahman PhD , Pavel Gershkovich PhD , Maria Marlow PhD","doi":"10.1016/j.nano.2023.102664","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102664","url":null,"abstract":"<div>We investigated how the biodistribution of cannabidiol (CBD) within the central nervous system (CNS) is influenced by two different formulations, an oil-in-water (O/W) nanoemulsion and polymer-coated nanoparticles (PCNPs). We observed that both CBD formulations administered were preferentially retained in the spinal cord, with high concentrations reaching the brain within 10 min of administration. The CBD nanoemulsion reached Cmax in the brain at 210 ng/g within 120 min (Tmax), whereas the CBD PCNPs had a Cmax of 94 ng/g at 30 min (Tmax), indicating that rapid brain delivery can be achieved through the use of PCNPs. Moreover, the AUC0–4 h of CBD in the brain was increased 3.7-fold through the delivery of the nanoemulsion as opposed to the PCNPs, indicating higher retention of CBD at this site. Both formulations exhibited immediate anti-nociceptive effects in comparison to the respective blank formulations.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"49 ","pages":"Article 102664"},"PeriodicalIF":5.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"2892277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Coatsome-replicon vehicles: Self-replicating RNA vaccines against infectious diseases 衣壳复制子载体:抗传染病的自我复制RNA疫苗

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-04-01 DOI: 10.1016/j.nano.2023.102655

Thomas Démoulins PhD , Kai Schulze PhD , Thomas Ebensen PhD , Navapon Techakriengkrai DVM, PhD , Teerawut Nedumpun DVM, PhD , Pavlos C. Englezou PhD , Markus Gerber Laboratory technician , Ruslan Hlushchuk MD, PhD , Darien Toledo MSc , Valentin Djonov MD, PhD , Stephan von Gunten MD, PhD , Kenneth C. McCullough PhD , Matthias Liniger PhD , Carlos A. Guzmán MD, PhD , Sanipa Suradhat DVM, PhD , Nicolas Ruggli DVM, PhD

{"title":"Coatsome-replicon vehicles: Self-replicating RNA vaccines against infectious diseases","authors":"Thomas Démoulins PhD , Kai Schulze PhD , Thomas Ebensen PhD , Navapon Techakriengkrai DVM, PhD , Teerawut Nedumpun DVM, PhD , Pavlos C. Englezou PhD , Markus Gerber Laboratory technician , Ruslan Hlushchuk MD, PhD , Darien Toledo MSc , Valentin Djonov MD, PhD , Stephan von Gunten MD, PhD , Kenneth C. McCullough PhD , Matthias Liniger PhD , Carlos A. Guzmán MD, PhD , Sanipa Suradhat DVM, PhD , Nicolas Ruggli DVM, PhD","doi":"10.1016/j.nano.2023.102655","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102655","url":null,"abstract":"<div>Herein, we provide the first description of a synthetic delivery method for self-replicating replicon RNAs (RepRNA) derived from classical swine fever virus (CSFV) using a Coatsome-replicon vehicle based on Coatsome® SS technologies. This results in an unprecedented efficacy when compared to well-established polyplexes, with up to ∼65 fold-increase of the synthesis of RepRNA-encoded gene of interest (GOI). We demonstrated the efficacy of such Coatsome-replicon vehicles for RepRNA-mediated induction of CD8 T-cell responses in mice. Moreover, we provide new insights on physical properties of the RepRNA, showing that the removal of all CSFV structural protein genes has a positive effect on the translation of the GOI. Finally, we successfully engineered RepRNA constructs encoding a porcine reproductive and respiratory syndrome virus (PRRSV) antigen, providing an example of antigen expression with potential application to combat viral diseases. The versatility and simplicity of modifying and manufacturing these Coatsome-replicon vehicle formulations represents a major asset to tackle foreseeable emerging pandemics.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"49 ","pages":"Article 102655"},"PeriodicalIF":5.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3341944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoparticle albumin-bound paclitaxel-based neoadjuvant regimen: A promising treatment option for HER2-low-positive breast cancer 纳米颗粒白蛋白结合紫杉醇为基础的新辅助方案:her2低阳性乳腺癌的一个有希望的治疗选择

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-04-01 DOI: 10.1016/j.nano.2023.102666

Wenjie Shi M.M , Xinyu Wan M.M , Ye Wang M.M , Jinzhi He M.M , Xiaofeng Huang M.M , Yinggang Xu M.M , Weiwei Zhang M.M , Rui Chen M.M , Lexin Wang M.M , Ran Zheng M.M , Lingjun Ma M.M , Xuan Li M.M , Lu Xu M.D , Xiaoming Zha M.D , Jue Wang M.D

{"title":"Nanoparticle albumin-bound paclitaxel-based neoadjuvant regimen: A promising treatment option for HER2-low-positive breast cancer","authors":"Wenjie Shi M.M , Xinyu Wan M.M , Ye Wang M.M , Jinzhi He M.M , Xiaofeng Huang M.M , Yinggang Xu M.M , Weiwei Zhang M.M , Rui Chen M.M , Lexin Wang M.M , Ran Zheng M.M , Lingjun Ma M.M , Xuan Li M.M , Lu Xu M.D , Xiaoming Zha M.D , Jue Wang M.D","doi":"10.1016/j.nano.2023.102666","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102666","url":null,"abstract":"<div>This study aimed to compare the efficacy of neoadjuvant systemic therapy (NST) with solvent-based paclitaxel (Sb-P), liposomal paclitaxel (Lps-P), nanoparticle albumin-bound paclitaxel (Nab-P), and docetaxel in human epidermal growth factor receptor 2 (HER2)-low-positive and HER2-zero breast cancers. A total of 430 patients receiving 2-weekly dose-dense epirubicin and cyclophosphamide (EC) followed by 2-weekly paclitaxel (Sb-P, Lps-P, or Nab-P), or 3-weekly EC followed by 3-weekly docetaxel for NST were enrolled in the study. In HER2-low-positive patients, the pathological complete response (pCR) rate in Nab-P group was significantly higher than that in the other three paclitaxel groups (2.8 % in Sb-P group, 4.7 % in Lps-P group, 23.2 % in Nab-P group and 3.2 % in docetaxel group, p < 0.001). In HER2-zero patients, the pCR rate did not differ significantly among the four paclitaxel groups (p = 0.278). The NST regimen containing Nab-P could be considered a promising treatment option in HER2-low-positive breast cancer.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"49 ","pages":"Article 102666"},"PeriodicalIF":5.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3021357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Macrophage cell membrane infused biomimetic liposomes for glioblastoma targeted therapy 巨噬细胞膜注入仿生脂质体用于胶质母细胞瘤靶向治疗

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-04-01 DOI: 10.1016/j.nano.2023.102663

D. Mendanha MSc, J. Vieira de Castro PhD, M.R. Casanova PhD, S. Gimondi MSc, H. Ferreira PhD, N.M. Neves PhD

{"title":"Macrophage cell membrane infused biomimetic liposomes for glioblastoma targeted therapy","authors":"D. Mendanha MSc, J. Vieira de Castro PhD, M.R. Casanova PhD, S. Gimondi MSc, H. Ferreira PhD, N.M. Neves PhD","doi":"10.1016/j.nano.2023.102663","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102663","url":null,"abstract":"<div>Glioblastoma (GBM) is a highly aggressive malignant brain tumor currently without an effective treatment. Inspired by the recent advances in cell membrane biomimetic nanocarriers and by the key role of macrophages in GBM pathology, we developed macrophage membrane liposomes (MML) for GBM targeting. For the first time, it was assessed the role of macrophage polarization states in the effectiveness of these drug delivery systems. Interestingly, we observed that MML derived from M2 macrophages (M2 MML) presents higher uptake and increased delivery of the anticarcinogenic drug doxorubicin compared to M1 macrophage-derived nanocarriers (M1 MML) and control liposomes (CL). Moreover, the lowest uptake by macrophages of MML reveals promising immune escaping properties. Notably, M2 macrophages unveiled a higher expression of integrin CD49d, a crucial protein involved in the bilateral communication of macrophages with tumor cells. Therefore, our findings suggest the potential of using M2 macrophage membranes to develop novel nanocarriers targeting GBM.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"49 ","pages":"Article 102663"},"PeriodicalIF":5.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"2892276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

A dandelion-like nanomedicine via hierarchical self-assembly for synergistic chemotherapy and photo-dynamic cancer therapy 一种蒲公英样的纳米药物，通过分层自组装用于协同化疗和光动力癌症治疗

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-04-01 DOI: 10.1016/j.nano.2023.102660

Binbin Liang PhD , Yuhang Miao MSc , Liying Zhao MSc , Lan Fang PhD , Dawei Deng PhD

{"title":"A dandelion-like nanomedicine via hierarchical self-assembly for synergistic chemotherapy and photo-dynamic cancer therapy","authors":"Binbin Liang PhD , Yuhang Miao MSc , Liying Zhao MSc , Lan Fang PhD , Dawei Deng PhD","doi":"10.1016/j.nano.2023.102660","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102660","url":null,"abstract":"<div>The synergistic effect of chemotherapy and photo-dynamic therapy (PDT) is an effective way to improve the efficiency of tumor treatment. However, most synergistic therapeutic drugs have poor water solubility and stability, so it is difficult to achieve high therapeutic effects while avoiding the severe side effects. Herein, a unique dandelion-like nanomedicine (named as cRGDfk-CCPT-mCe6) was successfully synthesized using Ce6-loaded amphiphilic β-cyclodextrins (β-CD) doped lipid-based vesicles as the core (receptacle) and β-CD modified camptothecin (CPT) pro-drug as the flyable dandelion seeds. The β-CD modified CPT pro-drug was introduced into the core vesicles in succession via host-guest interaction between inter-molecular β-CD and CPT, and cRGDfk peptides were further introduced as the outermost layer (stigma) to enhance the internalization into cancer cells. CPT interacted with β-CD through glutathione (GSH)-cleavable disulfide bonds, which led to drug release in glutathione-rich cancer cells, just as spread of dandelion seeds in the wind. GSH consumption further disrupted the intracellular redox homeostasis of cancer cells through combined action of Ce6 with light irradiation and the synergistic anti-tumor effect was thus achieved, resulting in apoptosis of cancer cells. Therefore, the nanomedicine provides a facile and versatile anti-tumor strategy, as well as a persistent anti-cancer effects.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"49 ","pages":"Article 102660"},"PeriodicalIF":5.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1567232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The combinational nano-immunotherapy of ferumoxytol and poly(I:C) inhibits melanoma via boosting anti-angiogenic immunity 阿魏木糖醇和聚(I:C)联合纳米免疫疗法通过增强抗血管生成免疫抑制黑色素瘤

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-04-01 DOI: 10.1016/j.nano.2023.102658

Yunuo Zheng M.S. , Bo Jiang M.S. , Hongmei Guo B.S. , Zhonghai Zhang M.S. , Bo Chen Ph.D. , Zhengkui Zhang Ph.D. , Shaoyuan Wu Ph.D. , Jiaojiao Zhao Ph.D.

{"title":"The combinational nano-immunotherapy of ferumoxytol and poly(I:C) inhibits melanoma via boosting anti-angiogenic immunity","authors":"Yunuo Zheng M.S. , Bo Jiang M.S. , Hongmei Guo B.S. , Zhonghai Zhang M.S. , Bo Chen Ph.D. , Zhengkui Zhang Ph.D. , Shaoyuan Wu Ph.D. , Jiaojiao Zhao Ph.D.","doi":"10.1016/j.nano.2023.102658","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102658","url":null,"abstract":"<div>Angiogenesis plays a key role in the progression and metastasis of melanoma, and the pro-angiogenic effect of macrophages is one major reason for the failure of current anti-angiogenic therapies. Here, a nano-immunotherapy combining ferumoxytol and poly(I:C) (ferumoxytol/poly(I:C)) has been developed to boost the anti-angiogenic activities of macrophages to inhibit melanoma. Our findings demonstrated that ferumoxytol/poly(I:C) was a highly efficacious anti-tumor therapy with limited toxicity. Both in vivo and in vitro experiments indicated that this combination was successful in impeding angiogenesis. Ferumoxytol/poly(I:C) was demonstrated to reduce the viability of endothelial cells, thus hindering tube formation. Particularly, ferumoxytol/poly(I:C) was able to polarize macrophages to the M1 phenotype and decrease the expression of vascular endothelial growth factor, which in turn amplified the anti-angiogenic properties of ferumoxytol/poly(I:C). This combination of ferumoxytol/poly(I:C) nano-immunotherapy enriches the anti-angiogenic therapeutic nature of ferumoxytol and will shed new light on the treatment of melanoma.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"49 ","pages":"Article 102658"},"PeriodicalIF":5.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3341945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Carborane bearing pullulan nanogel-boron oxide nanoparticle hybrid for boron neutron capture therapy 含碳硼烷普鲁兰纳米凝胶-氧化硼纳米颗粒杂化硼中子俘获治疗

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-04-01 DOI: 10.1016/j.nano.2023.102659

Riku Kawasaki Ph. D. , Hidetoshi Hirano , Keita Yamana , Hinata Isozaki , Shogo Kawamura , Yu Sanada Ph. D. , Kaori Bando , Anri Tabata , Kouhei Yoshikawa , Hideki Azuma Ph. D. , Takushi Takata Ph. D. , Hiroki Tanaka Ph.D. , Yoshinori Sakurai Ph. D. , Minoru Suzuki M. D. , Naoki Tarutani Ph. D. , Kiyofumi Katagiri Ph. D. , Shin-ichi Sawada Ph. D. , Yoshihiro Sasaki Ph. D. , Kazunari Akiyoshi Ph. D. , Takeshi Nagasaki Ph. D. , Atsushi Ikeda Ph. D.

{"title":"Carborane bearing pullulan nanogel-boron oxide nanoparticle hybrid for boron neutron capture therapy","authors":"Riku Kawasaki Ph. D. , Hidetoshi Hirano , Keita Yamana , Hinata Isozaki , Shogo Kawamura , Yu Sanada Ph. D. , Kaori Bando , Anri Tabata , Kouhei Yoshikawa , Hideki Azuma Ph. D. , Takushi Takata Ph. D. , Hiroki Tanaka Ph.D. , Yoshinori Sakurai Ph. D. , Minoru Suzuki M. D. , Naoki Tarutani Ph. D. , Kiyofumi Katagiri Ph. D. , Shin-ichi Sawada Ph. D. , Yoshihiro Sasaki Ph. D. , Kazunari Akiyoshi Ph. D. , Takeshi Nagasaki Ph. D. , Atsushi Ikeda Ph. D.","doi":"10.1016/j.nano.2023.102659","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102659","url":null,"abstract":"<div>Boron neutron capture therapy shows is a promising approach to cancer therapy, but the delivery of effective boron agents is challenging. To address the requirements for efficient boron delivery, we used a hybrid nanoparticle comprising a carborane = bearing pullulan nanogel and hydrophobized boron oxide nanoparticle (HBNGs) enabling the preparation of highly concentrated boron agents for efficient delivery. The HBNGs showed better anti-cancer effects on Colon26 cells than a clinically boron agent, L-BPA/fructose complex, by enhancing the accumulation and retention amount of the boron agent within cells in vitro. The accumulation of HBNGs in tumors, due to the enhanced permeation and retention effect, enabled the delivery of boron agents with high tumor selectivity, meeting clinical demands. Intravenous injection of boron neutron capture therapy (BNCT) using HBNGs decreased tumor volume without significant body weight loss, and no regrowth of tumor was observed three months after complete regression. The therapeutic efficacy of HBNGs was better than that of L-BPA/fructose complex. BNCT with HBNGs is a promising approach to cancer therapeutics.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"49 ","pages":"Article 102659"},"PeriodicalIF":5.5,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1567233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

HPMA copolymer conjugated 5-aminolevulinic acid exhibits superior efficacy for photodynamic therapy with tumor-responsive and targeting properties HPMA共聚物共轭5-氨基乙酰丙酸在光动力治疗中具有优异的肿瘤反应性和靶向性

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-02-01 DOI: 10.1016/j.nano.2022.102636

Rayhanul Islam Ph.D. , Kevin Kotalík BSc. , Vladimír Šubr Ph.D. , Shanghui Gao M.Sc. , Jian-Rong Zhou Ph.D. , Kazumi Yokomizo Ph.D. , Tomáš Etrych Ph.D., DSc. , Jun Fang Ph.D., M.D.

{"title":"HPMA copolymer conjugated 5-aminolevulinic acid exhibits superior efficacy for photodynamic therapy with tumor-responsive and targeting properties","authors":"Rayhanul Islam Ph.D. , Kevin Kotalík BSc. , Vladimír Šubr Ph.D. , Shanghui Gao M.Sc. , Jian-Rong Zhou Ph.D. , Kazumi Yokomizo Ph.D. , Tomáš Etrych Ph.D., DSc. , Jun Fang Ph.D., M.D.","doi":"10.1016/j.nano.2022.102636","DOIUrl":"https://doi.org/10.1016/j.nano.2022.102636","url":null,"abstract":"<div>In this study, we developed a nanoformulation of 5-aminolevulinic acid (5-ALA) for tumor-targeted photodynamic therapy, in which 5-ALA was conjugated with a biocompatible polymer N-(2-hydroxypropyl)methacrylamide (HPMA) through the hydrazone bond, i.e., P-ALA. P-ALA behaves as the nano-sized molecule with an average size of 5.5 nm in aqueous solution. P-ALA shows a largely increased release rate in acidic pH than physiological pH, suggesting the rapid release profile in acidic tumor environment. P-ALA did not show apparent cytotoxicity up to 0.1 mg/ml, however, under light irradiation, remarkable cell death was induced with the IC50 of 20–30 μg/ml. More importantly, we found significantly higher tumor accumulation of P-ALA than 5-ALA which benefit from its nano-size by taking advantage of the enhanced permeability and retention (EPR) effect. Consequently, P-ALA exhibited much improved in vivo antitumor efficacy without any apparent side effects. We thus anticipate the application of P-ALA as a nano-designed photosensitizer for anticancer photodynamic therapy.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"48 ","pages":"Article 102636"},"PeriodicalIF":5.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3021362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incorporation of glycyrrhizic acid and polyene phosphatidylcholine in lipid nanoparticles ameliorates acute liver injury via delivering p65 siRNA 在脂质纳米颗粒中掺入甘草酸和多烯磷脂酰胆碱可通过传递p65 siRNA改善急性肝损伤

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-02-01 DOI: 10.1016/j.nano.2022.102649

Qiming Yin PhD , Xiang Song PhD , Peng Yang MSc , Wen Yang MSc , Xinyu Li BSc , Xuejun Wang PhD , Shengqi Wang PhD

{"title":"Incorporation of glycyrrhizic acid and polyene phosphatidylcholine in lipid nanoparticles ameliorates acute liver injury via delivering p65 siRNA","authors":"Qiming Yin PhD , Xiang Song PhD , Peng Yang MSc , Wen Yang MSc , Xinyu Li BSc , Xuejun Wang PhD , Shengqi Wang PhD","doi":"10.1016/j.nano.2022.102649","DOIUrl":"https://doi.org/10.1016/j.nano.2022.102649","url":null,"abstract":"<div>Liver injury caused by hepatitis is the pathological basis of varied hepatic diseases with high morbidity and mortality. Although siRNA appears promising in therapeutics of hepatitis, efficient and safe delivery remains a challenge. In this study, we developed a new strategy of incorporating glycyrrhizic acid (GA) and polyene phosphatidylcholine (PPC) into lipid nanoparticles (GA/PPC-modified LNPs), which was capable of promoting cellular uptake, enhancing gene-silencing, reducing cytotoxicity and improving siRNA stability. GA/PPC-modified LNP and siRNA lipoplex targeting NF-κB, a key mediator of inflammation, mitigates acute liver injury, as assessed by liver histology, hematological and pro-inflammatory cytokine analysis. Furthermore, GA/PPC-modified LNPs reveal efficiently intracellular delivery of antisense oligonucleotides (ASOs) and mRNA inhibiting viral infection. In conclusion, GA/PPC-modified LNPs could be used as a promising delivery system for nucleic acid-based therapy.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"48 ","pages":"Article 102649"},"PeriodicalIF":5.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"2377484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxygen-independent alkyl radical nanogenerator enhances breast cancer therapy 不依赖氧的烷基自由基纳米发生器增强乳腺癌治疗

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-02-01 DOI: 10.1016/j.nano.2022.102630

Pilei Si PhD , Wenyan Yu PhD , Chengzhen Li M.M. , Haijun Chen M.M. , Enzhao Zhang M.M. , Jiaojiao Gu M.M. , Ruoyan Wang M.M. , Jinjin Shi PhD

{"title":"Oxygen-independent alkyl radical nanogenerator enhances breast cancer therapy","authors":"Pilei Si PhD , Wenyan Yu PhD , Chengzhen Li M.M. , Haijun Chen M.M. , Enzhao Zhang M.M. , Jiaojiao Gu M.M. , Ruoyan Wang M.M. , Jinjin Shi PhD","doi":"10.1016/j.nano.2022.102630","DOIUrl":"https://doi.org/10.1016/j.nano.2022.102630","url":null,"abstract":"<div>The hypoxic microenvironment of breast cancer substantially reduces oxygen-dependent free radical generation. Overexpression of glutathione (GSH) in tumor cells mitigates the impact of free radical generation. In this study, we designed and developed an oxygen-independent alkyl radical nanogenerator (copper monosulfide/2,2′-azabis(2-imidazoline) dihydrochloride@bovine serum albumin; CuS/AIPH@BSA) with spatiotemporally controlled properties and GSH consumption to enhance breast cancer therapy. We encapsulated the alkyl radical initiator, AIPH, in hollow mesoporous CuS nanoparticles with photothermal conversion effect and enveloped them in BSA. AIPH was released and decomposed to generate alkyl radicals in hypoxic breast cancer with the photothermal conversion effect of CuS under near-infrared laser irradiation. CuS consumed high GSH levels in tumor cells because it could form complex with GSH and thereby enhanced free radical treatment. In vivo and in vitro assays demonstrated the anti-tumor efficacy of the rationally designed free-radical nanogenerator in hypoxic microenvironment of breast cancer without showing systemic toxicity.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"48 ","pages":"Article 102630"},"PeriodicalIF":5.5,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3210032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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