四川大学学报(医学版)最新文献

{"title":"[Research Advances on Mechanisms and Interventions of DNA Methylation-Regulated Aging-Related Imbalance in Bone Metabolism].","authors":"Tailin He, Jie Li, Yifang Mei, Guozhi Xiao","doi":"10.12182/20260760301","DOIUrl":"10.12182/20260760301","url":null,"abstract":"<p><p>Aging can induce age-related bone diseases such as osteoporosis. DNA methylation, a core epigenetic regulatory mechanism, participate in the pathological process of aging-induced bone metabolism imbalance by modulating gene expression at the epigenetic level. Using S-adenosylmethionine as a methyl donor, it exhibits characteristics of hypomethylation in genomic repetitive regions and abnormal methylation in CpG islands of promoters of key bone metabolism genes with advancing age. The \"epigenetic clock\" constructed based on these features can accurately predict an individual's biological age. In bone metabolism, DNA methylation disrupts the osteoblast-osteoclast balance by targeting key factors. Such abnormalities are driven by aging-related inflammation and oxidative stress, while bone loss feedback exacerbates epigenetic disorders, forming a vicious cycle. Targeted intervention strategies have demonstrated significant potential in addressing bone metabolism-related issues. Low-dose DNA methyltransferase inhibitors can improve bone metabolism; nutrients such as folate and cobalamin maintain methylation homeostasis by optimizing one-carbon metabolism pathways; while CRISPR/dCas technology enables precise regulation in the cellular and animal levels, thereby affecting bone metabolism. However, existing strategies still face challenges such as off-target effects and low delivery efficiency. Future research needs to deepen mechanistic studies, optimize intervention methods, and promote their translation into clinical prevention and treatment of osteoporosis.</p>","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"57 4","pages":"905-914"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148881943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Molecular Mechanism of microRNA-429 Targeting <i>HMOX1</i> to Regulate Invasion, Migration, and Ferroptosis in Lung Adenocarcinoma Cells].","authors":"Xinyu Gao, Xiuying Gong, Shunfu Hou, Zhihan Zhang, Hongli Li, Xuejing Wang","doi":"10.12182/20260760605","DOIUrl":"10.12182/20260760605","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of microRNA-429 (miR-429) on the invasion, migration, and ferroptosis of lung adenocarcinoma (LUAD) cells, and to provide new insights into the clinical inhibition of LUAD invasion and migration and the promotion of ferroptosis.</p><p><strong>Methods: </strong>The expression profile of miR-429 in LUAD tissues was analyzed using the starBase database. Quantitative real-time PCR (qRT-PCR) was performed to assess miR-429 expression levels in normal human bronchial epithelial cells (the BEAS-2B cell line) and LUAD cells (the A549 and H1975 cell lines), and to evaluate the plasmid transfection efficiency. The effects of miR-429 on A549 cell proliferation, migration, and invasion were assessed using 5-ethynyl-2^'-deoxyuridine (EdU) incorporation assay, scratch wound healing assay, and Transwell invasion assay, respectively. Potential ferroptosis-related proteins targeted by miR-429 were predicted using bioinformatics databases, and the targeting relationship between miR-429 and heme oxygenase 1 (<i>HMOX1</i>) was validated by a dual-luciferase reporter assay. Further functional experiments were performed to examine the effect of miR-429 targeting <i>HMOX1</i> on the malignant phenotypes of A549 cells. The levels of reactive oxygen species (ROS) and lipid ROS (L-ROS) were measured to analyze the regulatory effects of miR-429 and <i>HMOX1</i> on oxidative stress in LUAD cells. The contents of malondialdehyde (MDA), glutathione (GSH), and ferrous ion (Fe²⁺) were measured to evaluate evaluate the effects of miR-429 and <i>HMOX1</i> on key ferroptosis-associated indicators in LUAD cells.</p><p><strong>Results: </strong>miR-429 expression was significantly higher in LUAD cell lines (A549 and H1975) compared with that in normal cells (BEAS-2B), with the highest expression level observed in A549 cells. Functional experiments demonstrated that miR-429 promoted A549 cell proliferation, migration, and invasion. The dual-luciferase assay confirmed that miR-429 directly targeted <i>HMOX1</i>, and their expression levels were negatively correlated in LUAD cells. <i>HMOX1</i> overexpression inhibited the proliferation, invasion, and migration of A549 cells, while miR-429 overexpression reversed these inhibitory effects. Mechanistically, miR-429 targeted and inhibited <i>HMOX1</i>, which led to reduced intracellular levels of ROS, L-ROS, MDA, and Fe²⁺ and increased GSH levels in A549 cells, thereby decreasing cellular sensitivity to ferroptosis.</p><p><strong>Conclusion: </strong>miR-429 promotes the invasion and migration of LUAD cells and inhibits ferroptosis by targeting and inhibiting <i>HMOX1</i>.</p>","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"57 4","pages":"1042-1051"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148881838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Age-Related Changes in Occlusion and the Clinical Management and Prevention Strategies].","authors":"Meiqing Wang","doi":"10.12182/20260760202","DOIUrl":"10.12182/20260760202","url":null,"abstract":"<p><p>Occlusion refers to the contact relationship between the upper and lower teeth. Its biomechanical properties influence motor function, most often in the orofacial and cervical regions, and the patient's psychological well-being as well. In this review, we summarized age-related changes in occlusion and the corresponding clinical management and preventive strategies from three perspectives-the functional characteristics of normal occlusion and their age-related changes, the effects of abnormal occlusion on health and the potential neurophysiological mechanisms involved, and age-related changes in occlusion and the corresponding preventive and therapeutic approaches. Abnormal wear of the occlusal surfaces alters the biomechanical relationship of occlusion. Animal studies have shown that abnormal biomechanical occlusion may result in the following problems: 1) periodontal trauma; 2) dysfunction of the orofacial and cervical muscles and vagus nerve-related visceral motor dysfunction through synaptic connections between neurons in the mesencephalic trigeminal nucleus and motor neurons in the trigeminal motor, facial, accessory, hypoglossal, and dorsal vagal nuclei; and 3) psychological responses such as anxiety, mediated via effects on neuronal activity in the lateral habenular nucleus. The wear coefficients of dental restorative prostheses differ from those of natural teeth. Long-term use of dental prostheses increases the risk of wear mismatch between natural and artificial teeth, which may lead to increased risk of occlusal interference. Tooth wear is the most common manifestation of age-related changes in occlusion. In addition to malocclusion, missing teeth, and pre-existing prostheses, many older patients also present with periodontal disease. Prolonged failure to replace missing teeth may lead to progressive occlusal disturbances, characterized by tilting of adjacent teeth and elongation of opposing teeth. When age-related wear is superimposed on malocclusion, periodontal disease, and missing teeth, it not only exacerbates the adverse health effects of abnormal occlusion but also substantially complicates treatment. Therefore, early diagnosis and treatment of malocclusion and periodontal diseases are essential to the prevention of situations where orthodontic intervention becomes extremely challenging in old age. Missing teeth should be restored promptly to prevent secondary occlusal disorders. For dental prostheses, careful attention should be paid to material selection and biomechanical design. Excessively hard materials should be avoided to prevent the development of interfering occlusal contacts. In addition, prostheses should be replaced regularly to reduce occlusal problems arising from uneven physiological wear and the associated secondary health consequences.</p>","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"57 4","pages":"959-965"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13542559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148897736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[CAMK2a Regulates Paclitaxel Resistance in Oral Squamous Cell Carcinoma Cells by Activating the ERK Signaling Pathway].","authors":"Junfeng Wang, Hongli Chen, Jiao Chen, Yun Feng, Yao Yuan, Ping Zhang","doi":"10.12182/20260760402","DOIUrl":"10.12182/20260760402","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the molecular mechanisms by which calcium signaling pathways regulate the paclitaxel resistance and the survival of oral squamous cell carcinoma (OSCC) cells.</p><p><strong>Methods: </strong>A paclitaxel-resistant OSCC cell model was established through continuous low-dose paclitaxel induction with dose escalation. The stability of the paclitaxel-resistant model was assessed using the Cell Counting Kit-8 (CCK-8) and imaging flow cytometry. RNA sequencing (RNA-seq) was performed to analyze differentially expressed genes and functional enrichment. Quantitative real-time PCR (qPCR) was conducted to analyze the differential expression of the <i>CAMK2a</i> gene. Protein expression levels were assessed by Western blot (WB). <i>CAMK2a</i> expression was inhibited using KN93 and <i>CAMK2a</i> small interfering RNA (siRNA).</p><p><strong>Results: </strong>A paclitaxel-resistant OSCC cell model was successfully established. Gene Ontology (GO) enrichment analysis of the sequencing data revealed differences in the calcium signaling pathways in resistant cells. The expression of multiple genes of the CAMK2 family was upregulated, and <i>CAMK2a</i> expression increased by 12% (<i>P</i> < 0.05). After inhibiting <i>CAMK2a</i> by treatment with KN93, an inhibitor, and <i>CAMK2a</i> siRNA-mediated gene knockdown, the proliferative capacity of paclitaxel-resistant cells decreased by 35% (<i>P</i> < 0.0001). Furthermore, the expression levels of phosphorylated Erk1/2 (p-Erk1/2) and its downstream signaling molecules also decreased following <i>CAMK2a</i> inhibition.</p><p><strong>Conclusion: </strong>CAMK2a regulates drug resistance and the proliferative capacity of paclitaxel-resistant cells by activating the MAPK-Erk1/2 pathway and represents a potential new therapeutic target for clinical treatment of drug-resistant patients.</p>","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"57 4","pages":"1032-1041"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534574/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148881872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Construction and Validation of a Risk Prediction Model for Catheter Blockage in Patients With Implantable Venous Access Ports].","authors":"Shuang Zhang, Suli Han, Ningjun Zhao","doi":"10.12182/20260760607","DOIUrl":"10.12182/20260760607","url":null,"abstract":"<p><strong>Objective: </strong>To explore the influencing factors of catheter blockage in patients with implantable venous access ports (IVAP) and to construct a risk prediction model.</p><p><strong>Methods: </strong>A total of 563 patients who underwent IVAP implantation from January 2022 to March 2024 were retrospectively enrolled and randomly divided into a modeling group (<i>n</i> = 394) and a validation group (<i>n</i> = 169) at a 7∶3 ratio. Univariate analysis and multivariate logistic regression (bidirectional stepwise regression) were used to identify independent influencing factors for catheter blockage. A nomogram prediction model was then constructed and evaluated in terms of discrimination, calibration, and clinical utility.</p><p><strong>Results: </strong>The incidence of catheter blockage was 12.79% (72/563). Multivariate Logistic regression showed that body mass index (BMI) ≥ 24 kg/m² (OR = 3.535, 95%CI: 1.500-8.329), diabetes mellitus (OR = 3.723, 95%CI: 1.540-8.999), malignancy (OR = 3.161, 95%CI: 1.327-7.526), history of thrombosis (OR = 3.324, 95%CI: 1.425-7.755), elevated D-dimer (OR = 547.088, 95%CI: 46.093-649.462), and catheter retention time ≥ 6 months (OR = 2.633, 95%CI: 1.060-6.543) were independent risk factors, while regular maintenance (OR = 0.200, 95%CI: 0.080-0.499) was an independent protective factor (all <i>P</i><0.05). The area under the receiver operating characteristic curve (AUC) of the nomogram model was 0.91 (95%CI: 0.87-0.96) in the modeling group and 0.94 (95%CI: 0.88-1.00) in the validation group. The Hosmer-Lemeshow test showed good calibration for both the modeling group (χ² = 10.782, <i>P</i> = 0.214) and the validation group (χ² = 3.744, <i>P</i> = 0.879). Decision curve analysis indicated a positive net clinical benefit.</p><p><strong>Conclusion: </strong>The IVAP catheter blockage risk prediction model constructed in this study demonstrates good predictive performance and clinical utility. It can assist healthcare providers in early identification of high-risk patients and implementation of individualized preventive interventions, thereby reducing the incidence of catheter blockage.</p>","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"57 4","pages":"1137-1144"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534598/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148881898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of Long-term Exposure to PM_2.5 and Its Chemical Components on Frailty Incidence in Middle-aged and Older Adults in China: A Prospective Cohort Study].","authors":"Kun Chen, Qian Liu, Chenchen Wu, Lihua Yang, Xiaojun Lin","doi":"10.12182/20260760103","DOIUrl":"10.12182/20260760103","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of long-term exposure to PM_2.5 and its chemical components on frailty risk among middle-aged and older adults in China.</p><p><strong>Methods: </strong>A prospective cohort study was conducted using data from the China Health and Retirement Longitudinal Study (CHARLS) collected between 2011 and 2020 and the Tracking Air Pollution (TAP) dataset. The frailty status of the participants was assessed using the frailty index. A time-dependent Cox proportional hazards model was applied to examine the associations between frailty risk and long-term exposure to PM_2.5 and its chemical components, including black carbon, organic matter, sulfate, nitrate, and ammonium.</p><p><strong>Results: </strong>A total of 9603 participants were included in the analysis. After adjusting for potential confounders, each interquartile range increase in the average PM_2.5 concentration during the preceding year was associated with a 10.0% higher risk of frailty (hazard ratio [HR] = 1.100, 95% CI: 1.015-1.193). For individual chemical components, each interquartile range increase in the one-year average concentrations of organic matter, black carbon, ammonium, sulfate, and nitrate, the participants were exposed to was associated with a 14.2% (HR = 1.142, 95% CI: 1.055-1.236), 13.1% (HR = 1.131, 95% CI: 1.041-1.229), 9.8% (HR = 1.098, 95% CI: 1.010-1.194), 8.8% (HR = 1.088, 95% CI: 0.996-1.187), and 8.1% (HR = 1.081, 95% CI: 0.996-1.172) higher risk of frailty, respectively.</p><p><strong>Conclusion: </strong>Long-term exposures to PM_2.5 and its chemical components, particularly black carbon, organic matter, and ammonium, were associated with a higher risk of frailty among middle-aged and older adults in China. Priority should be given to strengthening emission control of black carbon and organic aerosols to mitigate the public health burden of air pollution in the context of an aging society.</p>","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"57 4","pages":"985-992"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534608/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148881878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Development of a Risk Prediction Model for Moderate-to-Severe Complications Associated With Peripheral Intravenous Catheters in Older Patients].","authors":"Xiao Chen, Yuchen Guo, Zhimin Liang, Lingli Li","doi":"10.12182/20260760109","DOIUrl":"10.12182/20260760109","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To develop and validate a risk prediction model for moderate-to-severe complications associated with peripheral venous catheters (PVC) in older patients, and to provide evidence for precision-based clinical prevention and management.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;In this prospective cohort study, the clinical data of 750 older patients who underwent PVC placement for intravenous therapy between November 2023 and September 2024 were collected. Patients were stratified and randomly assigned to a training set (&lt;i&gt;n&lt;/i&gt; = 526) and a test set (&lt;i&gt;n&lt;/i&gt; = 224) at a ratio of 7∶3. Independent risk factors for moderate-to-severe PVC-related complications were identified by univariate and multivariate logistic regression analyses. A total of 4 predictive models were established-binary logistic regression (BLR), support vector machine (SVM), random forest (RF), and naive Bayes (NB). Model discrimination was assessed using the area under the receiver operating characteristic curve (AUC) and DeLong test. Model calibration was evaluated by calibration curves. Clinical net benefit was determined using decision curve analysis (DCA). Shapley additive explanations (SHAP) were used to visualize and interpret feature importance in the models.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;According to multivariate logistic regression analysis, functional status (mild dependence: odds ratio [OR] = 1.361, 95% CI: 1.031-1.802; moderate dependence: OR = 1.992, 95% CI: 1.291-3.061; severe dependence: OR = 3.060, 95% CI: 1.700-5.512), infusion extravasation (OR = 8.998, 95% CI: 4.286-18.887), the clinical department the patient was admitted to (Gastrointestinal Surgery: OR = 28.396, 95% CI: 7.537-106.990; Pancreatic Surgery: OR = 5.258, 95% CI: 1.177-23.499; Pancreatitis Center: OR = 5.974, 95% CI: 1.287-27.725), primary school or less formal education (OR = 2.525, 95% CI: 1.158-5.506), drinking history (OR = 1.970, 95% CI: 1.011-3.838), the edges of the dressing being curled up (OR = 1.458, 95% CI: 1.150-1.848), and the number of comorbidities (OR = 0.893, 95% CI: 0.821-0.972) were identified as independent risk factors for moderate-to-severe PVC-related complications in older patients, while 16G-22G catheters were identified as an independent protective factor (&lt;i&gt;P&lt;/i&gt; &lt; 0.05). Validation in the test set revealed that the RF model yielded the highest AUC value (0.891, 95% CI: 0.840-0.942). According to the DeLong test, the discrimination performance of the RF model was significantly superior to those of the BLR model (AUC = 0.808, 95% CI: 0.738-0.879) and the SVM model (AUC = 0.842, 95% CI: 0.774-0.909) (&lt;i&gt;P&lt;/i&gt; &lt; 0.05). SHAP analysis revealed that infusion extravasation, functional status, and the clinical department into which the patient was admitted were the top 3 features of the RF model. DCA showed that the RF model provided significant net clinical benefit within the threshold probability range of 0-0.8.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;In t","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"57 4","pages":"1153-1159"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148881920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Construction of a Multi-Factor Quantitative Scoring Model for Chromosome Karyotype Analysis].","authors":"Yi Lai, Qian Su, Hao Wang, Weihang Wu, Guolin He, Tao Li","doi":"10.12182/20260760505","DOIUrl":"10.12182/20260760505","url":null,"abstract":"<p><strong>Objective: </strong>To design a multi-factor quantitative evaluation model for chromosomal karyotype image quality to assist manual image selection, thereby improving the efficiency of image selection and the accuracy of karyotype analysis.</p><p><strong>Methods: </strong>Based on existing literature and clinical expert opinion, five indicators-chromosomal crossover degree, length, clarity, dispersion, and curvature-were selected as quantitative measures for constructing the image quality evaluation model, and corresponding algorithms were designed to score each indicator. Using expert manual review results as the gold standard, a dataset comprising chromosomal karyotype images labeled with whether the analytical results were correct was established. Univariate and multivariate analyses were performed to determine whether significant differences existed in each quantitative indicator between karyotypically correct and incorrect images. Indicators demonstrating significant associations were retained to construct a selection quality index and to establish a predictive model for karyotype analysis outcomes.</p><p><strong>Results: </strong>A total of 13 540 chromosomal images from 2708 patients underwent manual analysis, of which 416 images were identified by the expert panel as containing karyotype recognition errors. Measurement algorithms were developed and scores were calculated for each candidate image quality indicator. Univariate and multivariate analyses revealed that the scores for chromosomal curvature, crossover degree, clarity, and length significantly influenced the accuracy of karyotype recognition, whereas no statistically significant difference in dispersion score was observed between the correct and incorrect recognition groups (<i>P</i> = 0.133). Based on the identified contributing factors, a composite image quality index was constructed. Validation demonstrated that for every 1-point increase in the quality index, the risk of analytical error decreased by 8.4%. A declining trend in error rates was observed with increasing quality index scores; the error rate among images with a quality index below 75 was 3.88%, compared with 1.95% among images scoring 75 or above. A predictive model for the correctness of karyotype analysis outcomes, established based on the quality index, demonstrated favorable predictive performance with an area under the ROC curve (AUC) of 0.72 (95% CI: 0.67-0.77, <i>P</i> < 0.001).</p><p><strong>Conclusion: </strong>The image quality index and the predictive model for karyotype evaluation outcomes can effectively assist manual image selection in chromosomal karyotype analysis.</p>","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"57 4","pages":"1187-1194"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148881967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Prognostic Outcomes and Influencing Factors in Children With Severe Pneumonia Complicated by Mycoplasma Infection].","authors":"Chen Zhou, Yan Yi, Yun Tan, Xueping Jiang, Yangcan Ming","doi":"10.12182/20260760206","DOIUrl":"10.12182/20260760206","url":null,"abstract":"<p><strong>Objective: </strong>To investigate changes in the levels of serum amyloid-A (SAA) and coagulation indicators in children with severe pneumonia complicated by mycoplasma infection and their relationship with the prognosis.</p><p><strong>Methods: </strong>A total of 300 children with severe pneumonia were retrospectively enrolled. All the participants were admitted to the Department of Pediatrics of our hospital and received treatment there between July 2022 and December 2024. The patients were divided into a non-mycoplasma infection group (<i>n</i> = 180) and a mycoplasma infection group (<i>n</i> = 120) according to the presence or absence of mycoplasma infection. Then, children with severe pneumonia complicated by mycoplasma infection were further divided into a favorable prognosis subgroup (<i>n</i> = 85) and a poor prognosis subgroup (<i>n</i> = 35). Demographic and clinical data were collected for all participants. Logistic regression analysis was performed to determine whether age, SAA, fibrinogen (Fib), lung ultrasound (LUS) score, and other variables were influencing factors associated with prognosis, and their diagnostic performance was evaluated using receiver operating characteristic (ROC) curve.</p><p><strong>Results: </strong>The SAA Fib levels and LUS scores of the participants in the mycoplasma infection group were all higher than those in the non-mycoplasma group (all <i>P</i> < 0.05). Spearman correlation analysis showed that serum SAA levels, Fib levels, and LUS scores were positively correlated with both the occurrence of mycoplasma infection and poor prognosis after mycoplasma infection in children with severe pneumonia (all <i>P</i> < 0.05). The ROC curve analysis showed that the combined use of SAA, Fib, and LUS score predicted mycoplasma infection with an area under the curve (AUC) of 0.928 (95% CI, 0.899-0.957). Through multivariate logistic regression analysis, age (odds ratio [OR], 0.053; 95% CI, 0.003-0.997), SAA (OR, 1.045; 95% CI, 1.003-1.085), Fib (OR, 1.757; 95% CI, 1.378-8.158), and LUS score (OR, 3.538; 95% CI, 1.480-8.457) were identified as influencing factors associated with prognosis in children with severe pneumonia complicated by mycoplasma infection (all <i>P</i> < 0.05). ROC curve analysis demonstrated that the AUC of age, SAA, Fib, and LUS score for predicting prognosis were 0.837 (95% CI, 0.764-0.856), 0.792 (95% CI, 0.701-0.884), 0.755 (95% CI, 0.655-0.909), and 0.917 (95% CI, 0.828-1.000), respectively. The combined model incorporating the 4 parameters achieved an AUC of 0.997 (95% CI, 0.992-1.000).</p><p><strong>Conclusion: </strong>Age, SAA, Fib, and LUS scores can be used as diagnostic indicators for assessing the prognosis of children with severe pneumonia complicated by mycoplasma infections. The combined model demonstrates the highest predictive performance and may contribute to improved clinical prognostic assessment.</p>","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"57 4","pages":"1107-1113"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148881809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Oral-Systemic Antiaging Mechanisms and Intervention Strategies Guided by Traditional Chinese Medicine Principle of Preventive Treatment of Disease].","authors":"Huangping Ai, Chuncai Li, Zhao Jin, Yuling Zuo","doi":"10.12182/20260760201","DOIUrl":"10.12182/20260760201","url":null,"abstract":"<p><p>Population aging has become a global issue, and aging-related diseases have emerged as a major public health challenge. Inflammatory aging-the fundamental pathological basis of aging-related diseases-is closely associated with oral health status. However, current research and clinical practice remain limited by bottleneck issues, such as the separation of oral and systemic prevention and treatment strategies and the lack of systematic approaches to break the oral-systemic vicious cycle. This review addresses both the mechanisms and intervention strategies of the bidirectional oral-systemic regulation in anti-aging. We propose that the oral cavity serves as a precursory warning window for systemic aging and provide an in-depth analysis of the underlying mechanisms by which the oral cavity and the whole body form a bidirectional vicious pathogenic cycle through the immune-inflammatory network. At the molecular level, 3 key pathways are highlighted, including the lipopolysaccharide (LPS)/Toll-like receptor 4 (TLR4)/nuclear factor kappa-B (NF-κB) axis, the advanced glycation end products (AGEs)/receptor for advanced glycation end products (RAGE) axis, and the oral-brain neural feedback pathway. On this basis and under the guidance of the traditional Chinese medicine (TCM) principle of preventive treatment of disease, a three-tier collaborative intervention system covering the entire life course is proposed. Supported by multidisciplinary collaboration, a new integrated model incorporating early warning, intervention, and rehabilitation is established, thereby providing a comprehensive solution that integrates the holistic approach of TCM with modern life sciences to address the challenges of population aging.</p>","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"57 4","pages":"925-932"},"PeriodicalIF":0.0,"publicationDate":"2026-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13534583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148881858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}