四川大学学报(医学版)最新文献

{"title":"[Mechanisms of Wandai Decoction in Improving Vaginal Flora of Vulvovaginal Candidiasis of the Spleen Deficiency and Excessive Dampness Type: A Study Based on Metagenomics and Metabolomics].","authors":"Yue Han, Su Shen, Jie Fu, Qingling Ren","doi":"10.12182/20250160110","DOIUrl":"10.12182/20250160110","url":null,"abstract":"<p><strong>Objective: </strong>To explore the mechanism by which Wandai Decoction prevents and treats vulvovaginal candidiasis (VVC) of the spleen deficiency and excessive dampness type and restores the vaginal flora structure, and to identify the potential metabolic pathways involved using metagenomics and metabolomics.</p><p><strong>Methods: </strong>Twenty VVC patients who met the inclusion criteria were randomly assigned to a Wandai Decoction group and a fluconazole group (<i>n</i> = 10 in each group). Subjects in the fluconazole group were given a single oral dose of 150 mg fluconazole, while those in the Wandai Decoction group took the Wandai Decoction orally for 14 days. The vulvovaginal signs and symptoms (VSS) scores of both patient groups were evaluated before and after treatment. Vaginal secretions were collected before and after treatment. The Illumina sequencing and the liquid chromatography with tandem mass spectrometry (LC-MS/MS) platform were used to conduct metagenomic and metabolomics analyses of the vaginal secretions, respectively.</p><p><strong>Results: </strong>The VSS score results showed that the VSS scores of both groups decreased after treatment compared with those before treatment (<i>P</i> < 0.01), and there was no statistically significant difference in the VSS scores between the two groups after treatment. Metagenomics results showed that, after treatment, the vaginal microbial communities in the Wandai Decoction group were of CST Ⅱ and Ⅴ types (predominated by <i>Lactobacillus gasseri</i> and <i>Lactobacillus jensenii</i>), while those in the fluconazole group were <i>Lactobacillus_intestinalis</i> and <i>Streptococcus_sp._oral_</i> <i>taxon_</i>431. KEGG functional enrichment analysis results showed that, in terms of the cell cycle and meiosis functions of <i>Candida albicans</i>, statistically significant differences between the Wandai Decoction and fluconazole groups were observed (<i>P</i> < 0.05). Metabolomic analysis identified 120 differential metabolites between the two groups after treatment. The results of KEGG metabolic pathway enrichment analysis of differential metabolites showed that the Wandai Decoction might be significantly superior to fluconazole in improving local vaginal metabolic pathways of α-linolenic acid, glycerophospholipid metabolism, pentose and glucuronic acid interconversion, and arachidonic acid.</p><p><strong>Conclusion: </strong>The Wandai Decoction can improve the vaginal flora of VVC patients. It may be superior to fluconazole in the signaling pathways of the cell cycle and meiosis. The improvement of the vaginal flora by the Wandai Decoction may be associated with its effect on metabolic pathways of glycerophospholipid metabolism, pentose and glucuronic acid interconversion, and others in the vagina.</p>","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"56 1","pages":"68-73"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11914014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Characteristics of Flow and Heat Transfer in the Upper Respiratory Tract of Humans Under Nasal Obstructive Conditions].","authors":"Hui Pan, Jun Xie, Weiye Gao, Wei Chen","doi":"10.12182/20250160102","DOIUrl":"10.12182/20250160102","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the changes in the respiratory characteristics of the upper respiratory tract under nasal obstruction conditions, to identify the disparities in the flow-heat transfer processes within the respiratory tract, and to provide auxiliary support for the diagnosis and treatment of respiratory diseases.</p><p><strong>Methods: </strong>We established in this study a three-dimensional (3-D) model of the human upper respiratory tract based on computed tomography (CT) data. Computational fluid dynamics methods were used to compare and analyze the flow and heat transfer characteristics of the human upper respiratory tract under normal breathing and nasal obstructive conditions.</p><p><strong>Results: </strong>During normal breathing, the complex geometric shapes and large areas of heat transfer inside the nasal cavity of humans allowed the respiratory airflow in the posterior part of the nasal cavity to be warmed close to the body temperature. Under unilateral nasal obstruction, relatively high-speed gas and low-speed gas quickly mixed up and homogenized in the posterior part of the nasal cavity, and the airflow underwent sufficient heat transfer in the nasal cavity. Under bilateral nasal obstruction, air was inhaled through the mouth, and the flow velocity was significantly higher at the nasopharynx and the airway entrance compared to that of normal nasal air intake. Moreover, the heat transfer intensity of the airflow in the oral cavity was relatively weak, and the temperature rise of the airflow in the oral cavity was relatively small.</p><p><strong>Conclusion: </strong>A stable nasal breathing pattern plays an important role in maintaining human respiratory comfort and preventing respiratory diseases.</p>","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"56 1","pages":"215-221"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Role of Traditional Chinese Medicine in Pathogen-Induced Metabolic Reprogramming and Immune Suppression].","authors":"Yixiong Dang, Xin Sun, Jingwen Jiang","doi":"10.12182/20250160501","DOIUrl":"10.12182/20250160501","url":null,"abstract":"<p><p>Traditional Chinese medicine (TCM), an essential component of the traditional medicine practiced in China, has demonstrated unique therapeutic efficacy in combating infectious diseases caused by pathogens and various types of tumors. In particular, TCM plays a vital role in enhancing immune function, maintaining homeostasis, and improving metabolic balance. However, the complex ingredients used in TCM and its broad range of therapeutic targets present challenges for comprehensive analysis of the mechanisms involved. Moreover, the molecular and cellular mechanisms underlying TCM's effects remain underexplored, limiting its broader application in modern medicine. Recent studies have increasingly revealed that TCM can not only directly inhibit the activity of pathogens, such as bacteria, fungi, viruses, and tumor cells, but also exert profound effects on immune remodeling by regulating the metabolism of both pathogens and hosts. Therefore, a comprehensive understanding of the role of TCM in metabolic regulation is crucial for elucidating its anti-pathogenic effects. This review is focused on the metabolic pathways of pathogens and host metabolic reprogramming induced by pathogens. We systematically reviewed the mechanisms by which TCM regulates pathogen metabolism, influences pathogen-induced metabolic reprogramming in hosts, and mitigates immune suppression caused by pathogens. This review may provide new ideas for investigating the molecular mechanisms of TCM in disease development and progression.</p>","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"56 1","pages":"10-18"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11914019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Predicting Intensive Care Unit Mortality in Patients With Heart Failure Combined With Acute Kidney Injury Using an Interpretable Machine Learning Model: A Retrospective Cohort Study].","authors":"Xinyao Luo, Dingyuan Wan, Ke Wang, Yupei Li, Ruoxi Liao, Baihai Su","doi":"10.12182/20250160507","DOIUrl":"10.12182/20250160507","url":null,"abstract":"<p><strong>Objective: </strong>Heart failure (HF) complicated by acute kidney injury (AKI) significantly impacts patient outcomes, and it is crucial to make early predictions of short-term mortality. This study is focused on developing an interpretable machine learning model to enhance early prediction accuracy in such clinical scenarios.</p><p><strong>Methods: </strong>This retrospective cohort study utilized data from the Medical Information Mart for Intensive Care Ⅳ (MIMIC-Ⅳ, version 2.0) database. Data from the first 24 hours after admission to the ICU were extracted and divided into a training set (70%) and a validation set (30%). We utilized the SHapley Additive exPlanation (SHAP) method to interpret the workings of an extreme gradient boosting (XGBoost) model and identify key prognostic factors. The XGBoost model's predictive ability was evaluated against three other machine learning models using the area under the curve (AUC) metric, and its interpretation was enhanced using the SHAP method.</p><p><strong>Results: </strong>The study included 8028 patients with HF complicated by AKI. The XGBoost model outperformed the other models, achieving an AUC of 0.93 (95% confidence interval [CI]: 0.78-0.94; accuracy = 0.89), while neural network model showed the worst performance (AUC = 0.79, 95% CI: 0.77-0.82; accuracy = 0.82). Decision curve analysis showed the superior net benefit of the XGBoost model within the 9% to 60% threshold probabilities. SHAP analysis was performed to identify the top 20 predictors, with age (mean SHAP value 1.29) and Glasgow Coma Scale score (mean SHAP value 1.24) emerging as significant factors.</p><p><strong>Conclusions: </strong>Our interpretable model offers an enhanced ability to predict mortality risk in HF patients with AKI in ICUs. This model can be used to assist in formulating effective treatment plans and optimizing resource allocation.</p>","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"56 1","pages":"183-190"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11914016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Future-Oriented High-Quality Development of Integrated Traditional Chinese and Western Medicine].","authors":"Xiangmei Chen, Conghui Wang, Qinxiu Zhang, Yan Li, Yong Tang","doi":"10.12182/20250160401","DOIUrl":"10.12182/20250160401","url":null,"abstract":"<p><p>Integrated traditional Chinese and Western medicine refers to a clinical diagnosis and treatment model that incorporates the essence of traditional Chinese medicine in regard to treatment decisions based on syndrome differentiation and a reliance on modern western medicine as the basis of diagnosis and treatment. It is an organic combination of the two, with each bringing its own strengths into full play and complementing those of the other. After the founding of the People's Republic of China, the development of the integrated traditional Chinese and Western medicine has been increasingly valued. The unique advantages of the integration of traditional Chinese and Western medicine have been continuously utilized, resulting in fruitful achievements in theoretical inheritance and innovation and in the prevention and treatment of major diseases. Furthermore, integrated traditional Chinese and Western medicine has become a unique system of medicine of China. This article provides a review and elaboration of the entire process of the integration of traditional Chinese and Western medicine, including its significance, status of development, and achievements. It is suggested that, in the field of integrated traditional Chinese and Western medicine, efforts should made to delve into classic theories, identify the right entry points for integration, reconstruct and improve the theoretical system of integrated traditional Chinese and Western medicine by combining the cutting-edge achievements of modern medicine, and focus on enhancing clinical efficacy.</p>","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"56 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11914025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mechanisms of 6-Hydroxygen Genistein in the Treatment of Pulmonary Injury in High-Altitude Hypoxic Mice].","authors":"Chuan Ma, Xiaojuan Wang, Chenyu Yang, Shuyu Zhang, Baole Yang, Linlin Jing, Huiping Ma","doi":"10.12182/20250160205","DOIUrl":"10.12182/20250160205","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To investigate the mechanisms of 6-hydroxygenistein (6-OHG) in the treatment of high-altitude hypoxia-induced lung injury.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The intersection targets of 6-OHG and high-altitude hypoxia-induced lung injury were identified using databases, including Swiss Target Prediction, SuperPred, GeneCards, and OMIM. The STRING database and Cytoscape software were used to construct a protein interaction network for the intersection targets of drugs and diseases, and targets with degree values greater than the median were identified as key targets. GO and KEGG enrichment analyses of key targets were performed using the DAVID database to identify relevant signaling pathways. The Maestro 13.7 software was used for molecular docking validation. A large hypobaric hypoxic chamber was used to establish a high-altitude lung injury model in mice. A total of 42 male BALB/c mice were randomly assigned to 3 groups (&lt;i&gt;n&lt;/i&gt; = 14 in each group), including a normal control group, which was exposed to the environmental conditions at the altitude of 1400 m and received a single intraperitoneal injection of normal saline, a model group, which received a single intraperitoneal injection of normal saline, and a 6-OHG group, which received a single intraperitoneal injection of 6-OHG at 100 mg/kg. Then, 1 h after drug administration, mice in the model and 6-OHG groups were placed in a large hypobaric hypoxic simulation chamber for animal experiments. Then, they ascended to an altitude of 8000 m at a speed of 10 m/s, remained in that environment for 24 h, and then descended to an altitude of 3500 m. Mice in the three groups were sacrificed, and their lung tissues were extracted to measure the water content in the lungs. Pathological changes were observed using HE staining, and the levels of malondialdehyde (MDA), H2O2, total superoxide dismutase (T-SOD), and glutathione (GSH) were measured. Western blot was performed to determine the expression levles of p-PI3K/PI3K, p-AKT/AKT, hypoxia-inducible factor 1α (HIF-1α), and vascular endothelial growth factor (VEGF) proteins.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Key targets such as serine/threonine protein kinase 1 (AKT1), HIF-1α, epidermal growth factor receptor (EGFR), matrix metalloproteinase 9 (MMP9), and peroxisome proliferator-activated receptor A (PPARA) were identified. GO and KEGG enrichment analyses showed that the targets of 6-OHG in the treatment of high altitude hypoxia-induced lung injury were mainly involved in PI3K/AKT, HIF-1α/VEGF, tumor necrosis factor (TNF), and other signaling pathways. The results of animal experiments demonstrated that compared with the model group, the 6-OHG group showed significant improvement in the pathological damage of lung tissues induced by high altitude hypoxia, presenting statistically significant differences in the levels of MDA, H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt;, GSH, and T-SOD (&lt;i&gt;P&lt;/i&gt; &lt; 0.01). The results of Western blot assay revealed ","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"56 1","pages":"222-229"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11914032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mechanisms of the Anti-Fibrotic Effect of Ginsenoside Rh₁ on Hepatic Fibrosis].","authors":"Xuan Chen, Sai Yang, Bo Nan, Jisheng Ma, Yanfang Wang","doi":"10.12182/20250160203","DOIUrl":"10.12182/20250160203","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To investigate whether ginsenoside Rh&lt;sub&gt;1&lt;/sub&gt; (G-Rh&lt;sub&gt;1&lt;/sub&gt;) can alleviate liver fibrosis induced by a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) and to explore its underlying mechanisms.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Male C57BL/6J mice were randomly divided into 6 groups (&lt;i&gt;n&lt;/i&gt; = 8 in each group), including a standard diet group (or the control group), a high-fat diet group (or the CDAHFD group), a silymarin group (given silymarin at 5 mg/kg), a low-dose G-Rh&lt;sub&gt;1&lt;/sub&gt; group (given G-Rh&lt;sub&gt;1&lt;/sub&gt; at 5 mg/kg), a medium-dose G-Rh&lt;sub&gt;1&lt;/sub&gt; group (given G-Rh&lt;sub&gt;1&lt;/sub&gt; at 10 mg/kg), and a high-dose G-Rh&lt;sub&gt;1&lt;/sub&gt; group (given G-Rh&lt;sub&gt;1&lt;/sub&gt; at 20 mg/kg). The control group was given a standard feed, while the other groups were fed CDAHFD for 7 weeks to establish the mouse model of liver fibrosis. Starting from the first week, the mice in the treatment groups were administered the corresponding drugs by intragastric gavage once daily for 7 weeks in succession. After the administration of the final drug treatment, the body mass and organ mass of the mice in different groups were measured, and the organ index was obtained according. Liver tissues were examined using HE staining, Sirius red staining, and immunohistochemistry (IHC) staining. Western blot was performed to measure α-smooth muscle actin (α-SMA) and transforming growth factor-β&lt;sub&gt;1&lt;/sub&gt; (TGF-β&lt;sub&gt;1&lt;/sub&gt;), two liver fibrosis-related proteins, and fibroblast growth factor 12 (FGF-12), a pathway-related protein. The serum biochemical indicators, including aspartate transferase (AST), alanine aminotransferase (ALT), total bilirubin (TBIL), and direct bilirubin (DBIL), were measured. Additionally, RAW246.7 cells were randomly divided into 5 groups, including a control group, a lipopolysaccharide (LPS) group, and 3 G-Rh&lt;sub&gt;1&lt;/sub&gt; treatment groups. The control group had only RAW246.7 cells in the culture medium. The other groups were given LPS (500 ng/mL), and the 3 treatment groups received G-Rh&lt;sub&gt;1&lt;/sub&gt; at 10, 20, and 40 μmol/L in addition. The supernatants from the 5 groups of RAW246.7 cells were collected and cocultured with HSC-T6 cells for 24 hours to observe and compare the effects of G-Rh&lt;sub&gt;1&lt;/sub&gt; and LPS on the expression of fibrosis-related proteins, including α-SMA, Col1a1, etc, in HSC-T6 cells and on the expression of fibrotic signaling pathway-related proteins, including fibroblast growth factor 12 (FGF-12) and signal transducer and activator of transcription 3 (STAT3)/phosphorylated STAT3 (p-STAT3), in RAW264.7 cells. Flow cytometry was conducted to analyze the phenotypes of RAW246.7 cells, and ELISA was performed to measure fibrosis-related factors, including monocyte chemoattractant protein-1 (MCP-1) and transforming growth factor-β (TGF-β).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;Compared with the control mice, the mice in the CDAHFD group exhibited obvious liver fibrosis. Compared with CDAHFD mice, mi","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"56 1","pages":"120-128"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11914007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Preparation of a Microneedle Patch Loaded With Sodium Houttuyfonate Combined With Erythromycin and Its Antiacne Effects].","authors":"Tingting Wu, Qingru Chen, Xuemei Lu","doi":"10.12182/20250160206","DOIUrl":"10.12182/20250160206","url":null,"abstract":"<p><strong>Objective: </strong>To prepare a dissolvable microneedle (MN) patch loaded with sodium houttuyfonate (SH) combined with erythromycin (ERY) (SH + ERY-MN patch), to characterize its properties, and to investigate its synergistic anti-acne effects and the mechanisms involved.</p><p><strong>Methods: </strong>The SH + ERY-MN patch was prepared by two-step centrifugation. The morphology and skin puncture performance of the microneedles were evaluated using scanning electron microscopy, puncture tests, and other methods. The combined antibacterial effect was assessed using the checkerboard assay. The in vitro anti-inflammatory effect of the SH + ERY-MN patch was evaluated by quantitative PCR (qPCR) and enzyme-linked immunosorbent assay (ELISA). The therapeutic effect of the SH + ERY-MN patch on acnes in mice was investigated using an animal model.</p><p><strong>Results: </strong>The microneedle patch prepared in the study had well-aligned microneedles, excellent needle shape, and good mechanical properties, which could effectively penetrate the skin to reach the superficial dermis. The minimum inhibitory concentration (MIC) of ERY against acne was 1.28 mg/mL. When ERY was combined with SH, the results showed that the fractional inhibitory concentration (FIC) was 0.375, indicating that the medication combination had a synergistic antibacterial effect. In particular, the MIC value of ERY in combination was 1/4 of that of ERY used alone. The results of <i>in vitro</i> qPCR showed that SH + ERY-MN could downregulate the production of interleukin (<i>IL</i>)-<i>1β</i>, <i>IL-18</i>, tumor necrosis factor α (<i>TNF-α</i>), inhibitor of κB (<i>I-κB</i>), Toll-like receptor 4 (<i>TLR4</i>), NLR family pyrin domain containing 3 (<i>NLRP3</i>), and cysteine-aspartic proteases (<i>Caspase-1</i>) induced by <i>Propionibacterium acnes</i> (PA) in cell culture supernatant (<i>P</i> < 0.05). According to the <i>in vivo</i> findings, SH + ERY-MN had a significant anti-acne effect and effectively alleviated cytokine-mediated inflammatory responses. The results of qPCR and ELISA showed that SH + ERY-MN could effectively reduce the levels of IL-1β, IL-18, NLRP3, and Caspase-1 in the serum of the mouse acne inflammation model (<i>P</i> < 0.01), and had good antibacterial and anti-inflammatory effects.</p><p><strong>Conclusion: </strong>SH + ERY-MN patch was successfully prepared and demonstrated obvious anti-acne effects. The SH + ERY-MN patch enhances the transdermal delivery of SH combined with ERY. It provides an innovative method for acne treatment and creates new possibilities for achieving effective drug delivery and improving therapeutic efficacy.</p>","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"56 1","pages":"268-276"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11914031/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Optimal Timing of Moxibustion Intervention for Anti-inflammatory and Analgesic Effects Based on a Chronic Inflammatory Pain Model].","authors":"Ruizhu Zhou, Liuxuan He, Shuai Hou, Sha Yang, Haiyan Yin, Shuguang Yu","doi":"10.12182/20250160605","DOIUrl":"10.12182/20250160605","url":null,"abstract":"<p><strong>Objective: </strong>Based on a complete Freund's adjuvant (CFA)-induced chronic inflammatory pain model, we compared and analyzed the differences in anti-inflammatory and analgesic effects of moxibustion intervention initiated at different timepoints, aiming to identify the optimal timing for moxibustion intervention. The goal is to establish standardized intervention protocols for basic research on the anti-inflammatory and analgesic effects of moxibustion.</p><p><strong>Methods: </strong>Male C57BL/6 mice were randomly divided into 3 groups based on the moxibustion initiation timepoints of 4, 7, and 10 d after modeling. Then, the mice in each group were randomly assigned to 3 subgroups, including a control group, a model group, and a moxibustion group, with 8 mice in each subgroup. Chronic inflammatory pain was induced by injecting 20 μL of CFA into the sole of the right hind paw. Moxibustion applied at the \"Zusanli\" acupoint for 30 minutes started on the 4th, 7th, and 10th days after modeling, and the intervention continued for 7 days. The latency of paw withdrawal to thermal radiation was measured to evaluate the pain threshold before modeling, after modeling, and on the 1st, 4th, and 7th days of treatment. Foot volume was measured to assess toe swelling before modeling, after modeling, and on the 1st and 7th days of treatment.</p><p><strong>Results: </strong>Compared with the control group, the model group exhibited a reduced pain threshold (<i>P</i> < 0.0001) and increased paw volume (<i>P</i> < 0.0001). Compared with the model group, the subgroups receiving moxibustion intervention initiated on the 4th, 7th, and 10th days post-modeling exhibited an increased pain threshold (<i>P</i> < 0.05, <i>P</i> < 0.0001). However, the paw volume of the subgroups receiving moxibustion intervention initiated on the 4th day post-modeling increased (<i>P</i> < 0.0001), while those of the subgroups receiving moxibustion intervention initiated on the 7th and 10th days post-modeling decreased (<i>P</i> < 0.0001). Among the intervention subgroups receiving moxibustion initiated on days 4, 7, and 10, the day 7 intervention-initiating subgroup showed significant increase in pain threshold (<i>P</i> < 0.05, <i>P</i> < 0.0001), and the day 7 and day 10 intervention-initiating subgroups showed significantly reduced paw volume (<i>P</i> < 0.0001).</p><p><strong>Conclusion: </strong>Considering both the analgesic and anti-inflammatory effects of moxibustion, day 7 post-modeling may be the optimal time for moxibustion to achieve effective anti-inflammatory and analgesic outcomes.</p>","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"56 1","pages":"137-142"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11914027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical Manifestations of Early-Onset Capillary Leak Syndrome in Patients With Multiple Organ Failure Due to Severe Acute Pancreatitis].","authors":"Xueying Wu, Lan Li, Jiahua Shi, Jie Li, Ziyu Li, Ziqi Lin, Tingting Liu, Tao Jin, Qing Xia","doi":"10.12182/20250160609","DOIUrl":"10.12182/20250160609","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;To investigate the early dynamic changes of biomarkers associated with capillary leak syndrome (CLS) in patients with severe acute pancreatitis (SAP) and their correlation with multiple organ failure (MOF).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;A total of 171 SAP patients admitted to the West China Centre of Excellence for Pancreatitis, West China Hospital, Sichuan University between September 1, 2019 and December 31, 2020 were enrolled for this study. The patients were divided into MOF and non-MOF groups based on the occurrence of MOF in the first 5 days of hospitalization, and were further divided into subgroups based on the presence of moderate-to-severe intra-abdominal hypertension (IAH). We performed dynamic monitoring of the blood biomarkers (hematocrit [HCT], blood urea nitrogen [BUN], and creatinine [Cr]), plasma proteins (albumin [Alb], total protein [TP], and non-albumin plasma proteins [NAPP]), and intra-abdominal pressure. Trends in these indicators across groups were analyzed comprehensively.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;No significant differences in baseline data between the two groups were observed. The baseline data of the 2 groups were comparable. The MOF group had significantly higher rates of persistent systemic inflammatory response syndrome (SIRS) lasting 48 hours (91.3% vs. 71.8%), ICU admission (70.4% vs. 17.6%), and length-of-stay ([32 ± 17.7] days vs. [19.0 ± 12.2] days) compared to those of the non-MOF group (&lt;i&gt;P&lt;/i&gt; &lt; 0.05). The incidences of respiratory, circulatory, and renal failures were higher in the MOF group than those in the non-MOF group, showing significant differences in circulatory failure (69% vs. 3.5%) and renal failure (65.5% vs. 3.5%) (&lt;i&gt;P&lt;/i&gt; &lt; 0.05). In the first 5 days of hospitalization, the MOF group showed significantly elevated BUN and Cr levels, while Alb and TP levels dropped rapidly upon admission and then gradually recovered. The NAPP level of the MOF group continued to decrease after admission, and on the third day after admission, the NAPP level was lower than that of the Non-MOF group, showing statistically significant difference (&lt;i&gt;P&lt;/i&gt; &lt; 0.001). The Alb/NAPP ratio of the MOF group decreased significantly on day 1 and then rapidly increased, showing significant differences between the groups on days 3 and 4 (&lt;i&gt;P&lt;/i&gt; = 0.001). Subgroup analysis of MOF patients with moderate-to-severe IAH revealed similar trends in the dynamic changes and the overall changes in the indicators, and the difference was even more pronounced. The mixed linear model showed that the average levels of HCT, BUN, Alb/NAPP, and Alb/TP were higher and increased over time in the MOF combined with IAP subgroup (&lt;i&gt;P&lt;/i&gt; &lt; 0.001).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;The CLS model of SAP patients is validated, confirming that CLS is a key factor in the progression from SIRS to MOF. The loss of NAPP is an early and important indicator of CLS persistence and progression to MOF. Additionally, mo","PeriodicalId":39321,"journal":{"name":"四川大学学报(医学版)","volume":"56 1","pages":"277-283"},"PeriodicalIF":0.0,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11914012/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}