MorphologiePub Date : 2024-04-11DOI: 10.1016/j.morpho.2024.100779
A.M. Shurrab , A.T. Shatarat , M.H. Al-Muhtaseb , D.H. Badran , M.A. Salameh , H.M. Al-lahham , R.K. Altaweel , I. Altarawneh , D. Al-Qattan
{"title":"The effect of irisin on the ultrastructure of the thoracic aorta in rat: A morphometric study","authors":"A.M. Shurrab , A.T. Shatarat , M.H. Al-Muhtaseb , D.H. Badran , M.A. Salameh , H.M. Al-lahham , R.K. Altaweel , I. Altarawneh , D. Al-Qattan","doi":"10.1016/j.morpho.2024.100779","DOIUrl":"https://doi.org/10.1016/j.morpho.2024.100779","url":null,"abstract":"<div><h3>Background</h3><p>One of the most recent hormones to be identified and isolated is irisin, extracted from mouse skeletal muscle in 2012. Irisin has been proven to alter blood pressure, which has an impact on blood vessels, enhance endothelial functions, and prevent injury to endothelial cells. The current study aimed to study the effect of irisin on the ultrastructure of the rat thoracic aorta using the transmission electron microscope (TEM).</p></div><div><h3>Materials and methods</h3><p>Twenty female rats were recruited for this study and divided into a control group (non-injected), and four experimental groups (injected groups) each consisting of 4 rats. The experimental groups were injected intraperitoneally with different doses of irisin (250<!--> <!-->ng/mL, 500<!--> <!-->ng/mL, 1000<!--> <!-->ng/mL, and 2000<!--> <!-->ng/mL) twice a week for 4<!--> <!-->weeks. Then, the descending thoracic aorta of all experimental rats were resected and proceeded with imaging.</p></div><div><h3>Results</h3><p>The results of this study showed a change in the thickness of the tunica intima, internal elastic lamina, elastic lamellae, and external elastic lamina concerning increasing injected irisin concentration. While there was a significant increase in the thickness of tunica media (<em>P</em> <!--><<!--> <!-->0.0001) and smooth muscle cells (<em>P</em> <!--><<!--> <!-->0.05). Also, the results showed a significant increase in the number of elastic lamellae in the tunica media (<em>P</em> <!--><<!--> <!-->0.0001).</p></div><div><h3>Conclusion</h3><p>Irisin had a major impact on the elasticity of the rat thoracic aorta wall, suggesting that it influences the growth factors of the wall and activates smooth muscle cells in addition to endothelial cells.</p></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140546105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hox-A2 protein expression in avian jaws cartilages and muscle primordia development","authors":"Stéphane Louryan, Myriam Duterre, Nathalie Vanmuylder","doi":"10.1016/j.morpho.2024.100780","DOIUrl":"https://doi.org/10.1016/j.morpho.2024.100780","url":null,"abstract":"<div><h3>Objective</h3><p>To elucidate the branchial origin of the articular and the square (homology of the malleus and the incus of mammals), we used immunohistochemistry to analyse the expression of the Hox-A2 protein during cephalogenesis in chickens.</p></div><div><h3>Materials and methods</h3><p>Immunohistochemistry on paraffin sections of embryos from stage HH16 to HH40.</p></div><div><h3>Results</h3><p>In addition to the columella (equivalent to the mammalian stapes), the joint between the articular and the quadrate bones, and the retro-articular process of the articular (homologous to the short process of the malleus) express Hox-A2, suggesting an intervention of the 2nd arch in their formation. However, we fortuitously observed very intense expression within the early muscle plate of the second arch, which then generalized to all cephalic muscles, and extended to the trunk's myotomes. In the cartilage, the presence of the protein disappeared at stage 35.</p></div><div><h3>Discussion and conclusion</h3><p>The present results, while confirming the contribution of the second arch to the development of avian equivalents of the mammalian ear ossicles, strongly suggest that the <em>Hox-A2</em> gene plays a role in muscle development, which remains to be elucidated by more sophisticated techniques.</p></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140546106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MorphologiePub Date : 2024-04-04DOI: 10.1016/j.morpho.2024.100778
Kumara Kaluarachchi , Lakshman Samaranayake
{"title":"The first report of the presence of collagen X in mammalian dentinal matrix","authors":"Kumara Kaluarachchi , Lakshman Samaranayake","doi":"10.1016/j.morpho.2024.100778","DOIUrl":"https://doi.org/10.1016/j.morpho.2024.100778","url":null,"abstract":"<div><p>Collagen X is an extracellular matrix protein, usually found in the hypertrophic cartilage destined to be mineralized. It is intimately associated with the mineralization process of the mammalian hard tissues, and particularly, regulating the compartmentalization of matrix components. Despite the fact that the dentine of the tooth is highly mineralized, there are no previous reports to indicate the presence of collagen X in this connective tissue. Here we report, for the first time, its presence in mammalian dentine based on micromorphological and immunohistochemical data. We hypothesize that the collagen X in dentine may in the long term arrest the progression of the mineralization front towards the soft tissue components of the pulp that are not destined to be mineralized.</p></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140347728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MorphologiePub Date : 2024-04-04DOI: 10.1016/j.morpho.2024.100777
Marco P. Barros Pinto
{"title":"The importance of schistocytes in a patient in VA-ECMO","authors":"Marco P. Barros Pinto","doi":"10.1016/j.morpho.2024.100777","DOIUrl":"https://doi.org/10.1016/j.morpho.2024.100777","url":null,"abstract":"<div><p>The presence of schistocytes can be responsible for spurious thrombocytosis and spuriously low red blood count (RBC). The hemoglobin concentration will be correct (as the method usually used, destroys the red cells and converts a substantial proportion of the hemoglobin to a stable pigment) but mean corpuscular hemoglobin (MCH) is falsely high. The platelets and RBC histograms of the full blood count analyzers play an important role in the identification of schistocytes and must be carefully analyzed before reporting the previously full blood count parameters. In patients in ECMO, where can be expected the presence of a small number of schistocytes, this evaluation is particularly important to avoid wrong clinical decisions.</p></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S128601152400016X/pdfft?md5=1564f4142590344ffa80bd4e34a90028&pid=1-s2.0-S128601152400016X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140347729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MorphologiePub Date : 2024-03-30DOI: 10.1016/j.morpho.2024.100776
W.G. Lira-Bandeira , M.B. Montello , E.C. Moretti , I.B. Da Silva , G.A. Lemos , J.B. Oliveira , J.B. Da Silva , E.G. De Oliveira
{"title":"Frequency and characterization of the accessory heads of the biceps brachii: A multicentric cadaveric study","authors":"W.G. Lira-Bandeira , M.B. Montello , E.C. Moretti , I.B. Da Silva , G.A. Lemos , J.B. Oliveira , J.B. Da Silva , E.G. De Oliveira","doi":"10.1016/j.morpho.2024.100776","DOIUrl":"https://doi.org/10.1016/j.morpho.2024.100776","url":null,"abstract":"<div><h3>Purpose</h3><p>The biceps brachii muscle (BBM) is a large and thick muscle on the ventral portion of the upper arm. The scientific literature reports the existence of extra heads.</p></div><div><h3>Objective</h3><p>The present work investigated the frequency of occurrence of BBM accessory head(s) in four different Anatomy Laboratories at universities in Northeast Brazil.</p></div><div><h3>Material and methods</h3><p>Once the upper limbs with an anatomical variation of the BBM heads were identified, the accessory head was evaluated for morphometric characterization (the length, width, and thickness).</p></div><div><h3>Results</h3><p>The sample consisted of 249 upper limbs and the outcomes were 26 members with BBM accessory heads (10.44% of the total sample). Among the 26 members studied was found a single accessory head in 22 (84.62%), two accessory heads in 3 (11.54%), and three accessory heads in 1 (3.85%). BBMs with one, two, and three accessory heads were found, with different origins. The average length, width, and thickness of these accessory heads were 169.39<!--> <!-->mm, 10.25<!--> <!-->mm, and 3.39<!--> <!-->mm, respectively.</p></div><div><h3>Conclusion</h3><p>The frequency of BBM accessory heads in a population of northeastern Brazil in this cadaveric study was 10.44%.</p></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140330938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MorphologiePub Date : 2024-03-21DOI: 10.1016/j.morpho.2024.100775
P. Rishi , P. Sharma , S. Jain , A. Jain , P. Kumar , D. Shetty
{"title":"Correlation of palatal anatomic characteristics with dermatoglyphic heterogeneity in different growth patterns","authors":"P. Rishi , P. Sharma , S. Jain , A. Jain , P. Kumar , D. Shetty","doi":"10.1016/j.morpho.2024.100775","DOIUrl":"https://doi.org/10.1016/j.morpho.2024.100775","url":null,"abstract":"<div><h3>Aim and objective</h3><p>The study aimed to explore the correlation between dermatoglyphic patterns and quantitative palatal anatomic variables in individuals with different growth patterns.</p></div><div><h3>Materials and method</h3><p>A cross-sectional study was conducted involving 126 healthy patients aged 17–25 years. Participants were divided into three groups based on growth patterns: average, vertical, and horizontal. Dermatoglyphic patterns were recorded using an optical fingerprint sensor, and palatal characteristics were measured using digital software. Palatal characteristics, including intercanine width, intermolar width, and palatal depth, were measured using digital software. The results were statistically analyzed.</p></div><div><h3>Results</h3><p>Significant differences were observed in ridge counts among the three growth patterns. The average growth pattern showed lower ridge counts compared to the vertical and horizontal growth patterns. Dermatoglyphic patterns, such as double loops and tented arches, were significantly higher in the horizontal growth pattern. Weak correlations were found between certain dermatoglyphic patterns and palatal characteristics, with simple arch patterns showing a negative correlation with inter-canine width and symmetrical whorl patterns showing a positive correlation with palatal depth. Loop patterns, spiral patterns, double loop patterns, symmetrical whorl, and simple arch patterns were significant predictors of growth patterns.</p></div><div><h3>Conclusion</h3><p>This study revealed distinct dermatoglyphic patterns and ridge counts among individuals with different growth patterns. Weak correlations were observed between dermatoglyphic patterns and palatal characteristics. However, the predictive value of dermatoglyphics for skeletal malocclusion requires further investigation. Understanding the relationships between dermatoglyphic patterns and craniofacial growth can provide valuable insights into genetic and developmental factors affecting dental and orthodontic conditions.</p></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140180726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MorphologiePub Date : 2024-03-08DOI: 10.1016/j.morpho.2024.100772
A. Franco , A.P. Cornacchia , D. Moreira , P. Miamoto , J. Bueno , J. Murray , D. Heng , S. Mânica , L. Porto , A. Abade
{"title":"Radiographic morphology of canines tested for sexual dimorphism via convolutional-neural-network-based artificial intelligence","authors":"A. Franco , A.P. Cornacchia , D. Moreira , P. Miamoto , J. Bueno , J. Murray , D. Heng , S. Mânica , L. Porto , A. Abade","doi":"10.1016/j.morpho.2024.100772","DOIUrl":"https://doi.org/10.1016/j.morpho.2024.100772","url":null,"abstract":"<div><p>The permanent left mandibular canines have been used for sexual dimorphism when human identification is necessary. Controversy remains whether the morphology of these teeth is actually useful to distinguish males and females. This study aimed to assess the sexual dimorphism of canines by means of a pioneering artificial intelligence approach to this end. A sample of 13,046 teeth radiographically registered from 5838 males and 7208 females between the ages of 6 and 22.99 years was collected. The images were annotated using Darwin V7 software. DenseNet121 was used and tested based on binary answers regarding the sex (male or female) of the individuals for 17 age categories of one year each (i.e. 6–6.99, 7.7.99… 22.22.99). Accuracy rates, receiver operating characteristic (ROC) curves and confusion matrices were used to quantify and express the artificial intelligence's classification performance. The accuracy rates across age categories were between 57–76% (mean: 68%<!--> <!-->±<!--> <!-->5%). The area under the curve (AUC) of the ROC analysis was between 0.58 and 0.77. The best performances were observed around the age of 12 years, while the worst were around the age of 7 years. The morphological analysis of canines for sex estimation should be restricted and allowed in practice only when other sources of dimorphic anatomic features are not available.</p></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140061925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MorphologiePub Date : 2024-03-07DOI: 10.1016/j.morpho.2024.100774
S. Mânica , R. Merdietio Boedi , H. Pandey , C. Shields
{"title":"Identifying teeth and tooth fragments from digital 3D models","authors":"S. Mânica , R. Merdietio Boedi , H. Pandey , C. Shields","doi":"10.1016/j.morpho.2024.100774","DOIUrl":"https://doi.org/10.1016/j.morpho.2024.100774","url":null,"abstract":"<div><p>Dental anatomy is an essential skill for human identification in forensic odontology. With the advent of technology enabling virtual autopsies, there is scope for virtual consultation by forensic odontologists especially when the expertise is unavailable but needed in zones of conflict or disasters. This study aimed to investigate potential benefits and challenges of identifying intact and damaged teeth from 3D scanned digital models. Ten 3D tooth models – nine permanent and deciduous human teeth and one animal tooth were uploaded on a hosting platform. A 3-part survey was circulated among 60 forensic odontologists with questions about demography (P1), tooth identification of the scanned 3D models (P2) and the perceived usefulness of 3D models for tooth identification (P3). This was the first time that a survey required the identification of individual human teeth (intact or not) and animal tooth combined. The response rate for study participation was 58%. Substantial agreement among participants was seen in the determination of tooth classification (i.e., molars, premolars) or non-human and tooth within the same tooth class (i.e., lateral incisors, second molar) (both k<!--> <!-->=<!--> <!-->0.61). The least agreement (k<!--> <!-->=<!--> <!-->0.21) was seen in identification of tooth according to the FDI notation with a mean accuracy of 0.34. While most responders correctly identified the animal tooth, most incorrect responses were seen in the identification of the intact third molar. While 3D-scanned teeth have the potential to be identified virtually, forensic odontologists should continuously test their skills in tooth morphology and dental anatomy of humans (damaged or not) and animals.</p></div>","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1286011524000134/pdfft?md5=1fd1533851d2ee8cd4aa5314d9d561d1&pid=1-s2.0-S1286011524000134-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140061926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A case of significant loss of mesenteric fat: Educational and clinical relevance","authors":"K.M. Parr , I.G. Damjanovic , L.S.W. Bale, Madeline M. Damjanovic","doi":"10.1016/j.morpho.2024.100771","DOIUrl":"https://doi.org/10.1016/j.morpho.2024.100771","url":null,"abstract":"","PeriodicalId":39316,"journal":{"name":"Morphologie","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1286011524000109/pdfft?md5=1519703e2dd490d911a0e1ee6a27bbd3&pid=1-s2.0-S1286011524000109-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140062570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}