IF 4.2 3区生物学

Methods Pub Date : 2025-05-21 DOI: 10.1016/j.ymeth.2025.05.009

Biqing Chen, Jiayin Gao, Haizhu Sun, Zhi Chen, Xiaohong Qiu

{"title":"Surface-enhanced Raman scattering (SERS) technology: Emerging applications in cancer imaging and precision medicine","authors":"Biqing Chen, Jiayin Gao, Haizhu Sun, Zhi Chen, Xiaohong Qiu","doi":"10.1016/j.ymeth.2025.05.009","DOIUrl":"10.1016/j.ymeth.2025.05.009","url":null,"abstract":"<div><div>Recent advancements in Surface-enhanced Raman Scattering (SERS) bioprobes have substantially enhanced their bioimaging capabilities for disease theranostics. This review systematically analyzes three categories of engineered SERS probes: noble metal nanostructures, metal oxide hybrids, and multifunctional composite materials, emphasizing their optimized designs for targeted tumor detection and image-guided surgery. Key developments include improved in vitro biosensing platforms for rapid tumor screening and advanced in vivo probes enabling real-time intraoperative imaging with molecular specificity. The integration of SERS with complementary modalities (fluorescence, photoacoustic, MRI) is critically examined as a strategy to overcome individual technical limitations and achieve multiscale tissue characterization. Technical progress in spatial resolution enhancement, multiplex biomarker detection, and biocompatibility optimization is quantitatively highlighted. Current challenges in signal consistency across biological systems and scalable probe manufacturing are discussed, proposing standardized evaluation frameworks as essential for clinical translation. This work establishes SERS as a multimodal imaging cornerstone for precision oncology, particularly in tumor margin delineation and metastatic lesion identification.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"241 ","pages":"Pages 67-93"},"PeriodicalIF":4.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144131959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing RNA Delivery: Practical insights into NeoLNPTM transfection reagent 增强RNA传递：对NeoLNPTM转染试剂的实际见解

IF 4.2 3区生物学

Methods Pub Date : 2025-05-21 DOI: 10.1016/j.ymeth.2025.05.007

Xiaobao Chen , Yan Wu , Li Liu , Wei Wang

引用次数: 0

Quorum sensing: An essential factor in culturing the human promyelocytic leukemia cell line HL-60 and its neutrophil-related functions 群体感应：人早幼粒细胞白血病HL-60细胞培养及其中性粒细胞相关功能的重要因素。

IF 4.2 3区生物学

Methods Pub Date : 2025-05-20 DOI: 10.1016/j.ymeth.2025.05.008

Danfeng Li , Tian Yang , Siyuan Ma , Xinwei Lyu , Cheng Hu , Jiayin Yan , Lihong Guo , Jiali Tan

{"title":"Quorum sensing: An essential factor in culturing the human promyelocytic leukemia cell line HL-60 and its neutrophil-related functions","authors":"Danfeng Li , Tian Yang , Siyuan Ma , Xinwei Lyu , Cheng Hu , Jiayin Yan , Lihong Guo , Jiali Tan","doi":"10.1016/j.ymeth.2025.05.008","DOIUrl":"10.1016/j.ymeth.2025.05.008","url":null,"abstract":"<div><div>HL-60 cells are frequently employed as a standard <em>in vitro</em> model for neutrophil research and extensively utilized. However, the cultivation of HL-60 cells presents a recurring challenge. Historically, cell culture density has been ignored in the consistency of culture conditions. Here, we optimized the culture protocol and explored the impact of culture density on HL-60 cells. Additionally, we investigated the differentiated rate and antibacterial potential of differentiated HL-60 (dHL-60) neutrophils across varying cell density cultures. The findings revealed a positive correlation between cell proliferation activity and cell density, suggesting that increased density facilitates enhanced cell proliferation. Furthermore, as the density of the cell culture increased, there was a concomitant rise in the differentiation rate of HL-60 cells into neutrophils upon stimulation. Importantly, this elevated density also led to significantly higher levels of mitochondrial reactive oxygen species (ROS) production and bacterial phagocytosis. Further investigation revealed that small extracellular vesicles (sEVs) are crucial communicator in quorum sensing within HL-60 cells. Supplementation of HL60-derived sEVs (hEVs) in low-density cell populations resulted in a restoration of cell proliferation, in dose-dependent tendency. Conversely, the inhibition of EV-secretion in HL-60 cells restrains cell growth and proliferation. Overall, our study not only optimized the HL-60 cell culture protocol but also elucidated the critical role of culture density in enhancing HL-60 cell proliferation and antibacterial activity. This finding offers a noteworthy consideration for <em>in vitro</em> experiments of HL-60 cells and suggests the involvement of a quorum sensing mechanism within the neutrophil microenvironment.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"241 ","pages":"Pages 94-102"},"PeriodicalIF":4.2,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144126136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of structured light scanning probe configuration on the 3D scanning accuracy of the maxillofacial region in a smiling state: An in vitro study 结构光扫描探针配置对微笑状态下颌面部区域三维扫描精度的影响：体外研究

IF 4.2 3区生物学

Methods Pub Date : 2025-05-16 DOI: 10.1016/j.ymeth.2025.05.006

Miaosheng Guan , Xiaoyu Wang , Xue Li , Yaqi Wang , Kun Yan , Ran Huo , Tao Song , Lin Liu , Hongbo Li

{"title":"The influence of structured light scanning probe configuration on the 3D scanning accuracy of the maxillofacial region in a smiling state: An in vitro study","authors":"Miaosheng Guan , Xiaoyu Wang , Xue Li , Yaqi Wang , Kun Yan , Ran Huo , Tao Song , Lin Liu , Hongbo Li","doi":"10.1016/j.ymeth.2025.05.006","DOIUrl":"10.1016/j.ymeth.2025.05.006","url":null,"abstract":"<div><div>Currently, single-unit, dual-unit, and triple-unit structured light 3D scanning technologies have become the predominant facial scanning methods. However, the impact of different unit strategies on facial scanning accuracy remains unclear. A standardized 3D facial model in a smiling state was established. Key point reference coordinates and 3D data were obtained using a coordinate measurement instrument and an industrial-grade laser 3D scanner. Three structured light scanning techniques (single-, dual-, triple-unit) were utilized to capture the 3D information of the model. Linear distance deviations and 3D surface deviations (trueness and precision) of the three scanning strategies were compared. The triple-unit scanning strategy exhibited the lowest deviation among 20 trueness indicators and 22 precision indicators for linear distance measurements (P < 0.05). Furthermore, the accuracy of the triple-unit strategy (trueness: 0.1607 ± 0.0201 mm, precision: 0.0161 ± 0.0112 mm) for overall facial scanning was significantly lower than that of the single-unit and dual-unit strategies, particularly in critical regions for oral and maxillofacial aesthetic analysis, such as the orbital, nasal, and perioral regions. The triple-unit structured light scanning strategy significantly enhances the accuracy of facial 3D scanning, particularly when acquiring 3D facial information from the midline and perioral regions. This in vitro study demonstrates that the triple-unit structured light 3D scanning strategy effectively improves the accuracy of facial scanning, especially in the oral-maxillofacial aesthetic regions. This approach provides a foundation and support for both preoperative planning and postoperative evaluation of aesthetic restoration.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"241 ","pages":"Pages 43-50"},"PeriodicalIF":4.2,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-filter based signed heterogeneous graph convolutional networks for predicting activating/inhibiting drug-target interactions 基于多滤波器的预测药物-靶标相互作用激活/抑制的签名异构图卷积网络。

IF 4.2 3区生物学

Methods Pub Date : 2025-05-15 DOI: 10.1016/j.ymeth.2025.05.005

Ming Chen , Haike Li , Yunhan Pan , Yinglong Dai , Xiujuan Lei , Yi Pan

{"title":"Multi-filter based signed heterogeneous graph convolutional networks for predicting activating/inhibiting drug-target interactions","authors":"Ming Chen , Haike Li , Yunhan Pan , Yinglong Dai , Xiujuan Lei , Yi Pan","doi":"10.1016/j.ymeth.2025.05.005","DOIUrl":"10.1016/j.ymeth.2025.05.005","url":null,"abstract":"<div><div>The prediction of mechanisms within drug-target interactions (DTIs) can boost the drug discovery process, which has traditionally relied on time-consuming and expensive laboratory experiments. Despite much more attention has been paid to predicting DTIs, but few studies focused on their activating/inhibiting mechanisms. In this work, we model DTIs on signed heterogeneous networks, through categorizing activating/inhibiting DTIs into signed links, and accordingly introducing the coherence/incoherence between drugs on a common target to construct signed drug-drug links. We propose a multi-filter based signed heterogeneous graph convolutional network (MFSHGCN) for drugs and targets embedding, via employing dual filters on both the signed drug-drug sub-graph and the signed DTI sub-graph to converge the spectral information from positive and negative edges. We further put forward an end-to-end framework to predict activation and inhibition within DTIs. The comparison results demonstrate the introduction of coherence/incoherence of drug pairs and the design of our multi-filter system can effectively improve the prediction metrics, even without relying on rich node information and interactions from drug pairs or target pairs. Case studies on breast cancer and lung cancer confirm the model's feasibility.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"241 ","pages":"Pages 51-58"},"PeriodicalIF":4.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An NGS-based approach for precise and footprint-free CRISPR-based gene editing in human stem cells 一种基于ngs的方法，用于人类干细胞中基于crispr的精确和无足迹基因编辑

IF 4.2 3区生物学

Methods Pub Date : 2025-05-13 DOI: 10.1016/j.ymeth.2025.05.004

Bert Vandendriessche , Jolien Huyghebaert , Kirsten Van Rossem , Tycho Canter Cremers , Kevin De Man , Ewa Sieliwonczyk , Hanne Boen , Dogan Akdeniz , Laura Rabaut , Jolien Schippers , Peter Ponsaerts , R. Frank Kooy , Bart Loeys , Dorien Schepers , Maaike Alaerts

{"title":"An NGS-based approach for precise and footprint-free CRISPR-based gene editing in human stem cells","authors":"Bert Vandendriessche , Jolien Huyghebaert , Kirsten Van Rossem , Tycho Canter Cremers , Kevin De Man , Ewa Sieliwonczyk , Hanne Boen , Dogan Akdeniz , Laura Rabaut , Jolien Schippers , Peter Ponsaerts , R. Frank Kooy , Bart Loeys , Dorien Schepers , Maaike Alaerts","doi":"10.1016/j.ymeth.2025.05.004","DOIUrl":"10.1016/j.ymeth.2025.05.004","url":null,"abstract":"<div><div>Precise gene editing with conventional CRISPR/Cas9 is often constrained by low <em>knock-in</em> (KI) efficiencies (≈ 2–20 %) in human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs). This limitation typically necessitates labour-intensive manual isolation and genotyping of hundreds of colonies to identify correctly edited cells. Fluorescence- or antibiotic-based enrichment methods facilitate the identification process but can compromise cell viability and genomic integrity. Here, we present a footprint-free editing strategy that combines low-density seeding with next-generation sequencing (NGS) to rapidly identify cell populations containing precisely modified clones. By optimising the transfection workflow and adhering to CRISPR/Cas9 KI design principles, we achieved high average editing efficiencies of 64 % in hiPSCs (introducing a Brugada syndrome-associated variant) and 51 % in hESCs (introducing a neurodevelopmental disorder (NDD)-associated variant). Furthermore, under suboptimal CRISPR design conditions, this approach successfully identified hESC clones carrying a second NDD-associated variant, despite average KI efficiencies below 1 %. Importantly, genomic integrity was preserved throughout subcloning rounds, as confirmed by Sanger sequencing and single nucleotide polymorphism (SNP) array analysis. Hence, this NGS-based enrichment strategy reliably identifies desired KI clones under both optimal and challenging conditions, reducing the need for extensive colony screening and offering an effective alternative to fluorescence- and antibiotic-based selection methods.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"241 ","pages":"Pages 33-42"},"PeriodicalIF":4.2,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robust temporal knowledge inference via pathway snapshots with liquid neural network 基于路径快照的液体神经网络鲁棒时间知识推理

IF 4.2 3区生物学

Methods Pub Date : 2025-05-09 DOI: 10.1016/j.ymeth.2025.05.003

Peifu Han , Jianmin Wang , Dayan Liu , Lin Liu , Tao Song

{"title":"Robust temporal knowledge inference via pathway snapshots with liquid neural network","authors":"Peifu Han , Jianmin Wang , Dayan Liu , Lin Liu , Tao Song","doi":"10.1016/j.ymeth.2025.05.003","DOIUrl":"10.1016/j.ymeth.2025.05.003","url":null,"abstract":"<div><div>Static graphs play a pivotal role in modeling and analyzing biological and biomedical data. However, many real-world scenarios—such as disease progression and drug pharmacokinetic processes—exhibit dynamic behaviors. Consequently, static graph methods often struggle to robustly address new environments characterized by complex and previously unseen relationship changes. Here, we propose a method for constructing temporal knowledge inference agents tailored to disease pathways, enabling effective relation reasoning beyond their training environment under complex shifts. To achieve this, we developed an imitation learning framework using liquid neural networks, a class of continuous-time neural models inspired by the brain function that are causal and adaptable to changing conditions. Our findings indicate that liquid agents can distill the essential tasks from knowledge graph inputs while accounting temporal evolution, thereby enabling the transfer of temporal skills to novel time nodes. Compared to state-of-the-art deep reinforcement learning agents, experiments demonstrate that temporal robustness in decision-making emerges uniquely in liquid networks.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"241 ","pages":"Pages 24-32"},"PeriodicalIF":4.2,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical assay for the quantification of anticancer drugs and their inhibition mechanism 电化学法定量测定抗癌药物及其抑制机制

IF 4.2 3区生物学

Methods Pub Date : 2025-05-08 DOI: 10.1016/j.ymeth.2025.05.002

Ricardo Jose Branco Leote , Caroline G. Sanz , Victor C. Diculescu , Madalina Maria Barsan

{"title":"Electrochemical assay for the quantification of anticancer drugs and their inhibition mechanism","authors":"Ricardo Jose Branco Leote , Caroline G. Sanz , Victor C. Diculescu , Madalina Maria Barsan","doi":"10.1016/j.ymeth.2025.05.002","DOIUrl":"10.1016/j.ymeth.2025.05.002","url":null,"abstract":"<div><div>Overexpression of pyruvate kinase (PyK) is linked to many kinds of malignant tumors, representing therefore one of the most promising therapeutic targets for cancer treatment. Inhibition of PyK slows down tumor growth or causes tumor cell death, minimizing cancer cell proliferation, and understanding inhibitor mechanism of action can significantly improve cancer therapy. The present work describes the use of an amperometric bienzymatic biosensor, based on PyK and pyruvate oxidase (PyOx), in enzyme inhibition studies of four kinase inhibitors, CPG77675, Nilotinib, Ruxolitinib, Cerdulatinib. Their inhibition mechanism is studied and discussed in detail, with a thorough evaluation of their enzyme-inhibitor complex binding constants (<em>K<sub>i</sub></em>) and the inhibitor concentration required for 50% inhibition <em>(IC<sub>50</sub></em>), employing standard inhibition procedure graphical methods. The biosensor is successfully applied for the quantification of the inhibitors by fixed potential amperometry, with excellent detection limit values in the pM range. It is the first detection method reported for the anticancer drugs CPG77675 and Cerdulatinib. The electrochemical assay based on the biosensor brings several advantages over the available assay kits for high-throughput screening (HTS) of kinase inhibitors, namely: low cost, easy operability and robustness demonstrated by biosensor high reproducibility and both operational and storage stability, offering an opportunity to discover new inhibitors and optimize their therapeutic index.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"241 ","pages":"Pages 13-23"},"PeriodicalIF":4.2,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optical pooled screening for the discovery of regulators of the alternative lengthening of telomeres pathway 光学池筛选发现的调节端粒延长途径的选择

IF 4.2 3区生物学

Methods Pub Date : 2025-05-03 DOI: 10.1016/j.ymeth.2025.05.001

Isabel Quintanilla , Benura Azeroglu , Md Abdul Kader Sagar , Travis H. Stracker , Eros Lazzerini Denchi , Gianluca Pegoraro

{"title":"Optical pooled screening for the discovery of regulators of the alternative lengthening of telomeres pathway","authors":"Isabel Quintanilla , Benura Azeroglu , Md Abdul Kader Sagar , Travis H. Stracker , Eros Lazzerini Denchi , Gianluca Pegoraro","doi":"10.1016/j.ymeth.2025.05.001","DOIUrl":"10.1016/j.ymeth.2025.05.001","url":null,"abstract":"<div><div>Telomere elongation is essential for the proliferation of cancer cells. Telomere length control is achieved either by the activation of the telomerase enzyme, or by the recombination-based Alternative Lengthening of Telomeres (ALT) pathway. ALT is active in about 10–15% of human cancers, but its molecular underpinnings remain poorly understood, preventing the discovery of potential novel therapeutic targets. Pooled CRISPR-based functional genomic screens enable the unbiased discovery of molecular factors involved in cancer biology. Recently, Optical Pooled Screens (OPS) have significantly extended the capabilities of pooled functional genomics screens to enable sensitive imaging-based readouts at the single cell level and large scale. To gain a better understanding of the ALT pathway, we developed a novel OPS assay that employs telomeric native DNA FISH (nFISH) as an optical quantitative readout to measure ALT activity. The assay uses standard OPS protocols for library preparation and sequencing. As a critical element, an optimized nFISH protocol is performed before in situ sequencing to maximize the assay performance. We show that the modified nFISH protocol faithfully detects changes in ALT activity upon CRISPR knock-out (KO) of the <em>FANCM</em> and <em>BLM</em> genes, which were previously implicated in ALT. Overall, the OPS-nFISH assay is a reliable method that can provide deep insights into the ALT pathway in a high-throughput format.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"241 ","pages":"Pages 1-12"},"PeriodicalIF":4.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR revolution: Unleashing precision pathogen detection to safeguard public health and food safety CRISPR革命：开启病原体精准检测，保障公众健康和食品安全

IF 4.2 3区生物学

Methods Pub Date : 2025-04-29 DOI: 10.1016/j.ymeth.2025.04.018

Jacob Tizhe Liberty , Sabri Bromage , Endurance Peter , Olivia C. Ihedioha , Fatemah B. Alsalman , Tochukwu Samuel Odogwu

{"title":"CRISPR revolution: Unleashing precision pathogen detection to safeguard public health and food safety","authors":"Jacob Tizhe Liberty , Sabri Bromage , Endurance Peter , Olivia C. Ihedioha , Fatemah B. Alsalman , Tochukwu Samuel Odogwu","doi":"10.1016/j.ymeth.2025.04.018","DOIUrl":"10.1016/j.ymeth.2025.04.018","url":null,"abstract":"<div><div>Foodborne pathogens represent a significant challenge to global food safety, causing widespread illnesses and economic losses. The growing complexity of food supply chains and the emergence of antimicrobial resistance necessitate rapid, sensitive, and portable diagnostic tools. CRISPR technology has emerged as a transformative solution, offering unparalleled precision and adaptability in pathogen detection. This review explores CRISPR’s role in addressing critical gaps in traditional and modern diagnostic methods, emphasizing its advantages in sensitivity, specificity, and scalability. CRISPR-based diagnostics, such as Cas12 and Cas13 systems, enable rapid detection of bacterial and viral pathogens, as well as toxins and chemical hazards, directly in food matrices. Their integration with isothermal amplification techniques and portable biosensors enhances field applicability, making them ideal for decentralized and real-time testing. Additionally, CRISPR’s potential extends beyond food safety, contributing to public health efforts by monitoring antimicrobial resistance and supporting One Health frameworks. Despite these advancements, challenges remain, including issues with performance in complex food matrices, scalability, and regulatory barriers. This review highlights future directions, including AI integration for assay optimization, the development of universal CRISPR platforms, and the adoption of sustainable diagnostic solutions. By tackling these challenges, CRISPR has the potential to redefine global food safety standards and create a more resilient food system. Collaborative research and innovation will be critical to fully unlocking its transformative potential in food safety and public health.</div></div>","PeriodicalId":390,"journal":{"name":"Methods","volume":"240 ","pages":"Pages 180-194"},"PeriodicalIF":4.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143907880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methods最新文献