Enhancing RNA Delivery: Practical insights into NeoLNPTM transfection reagent

IF 4.3 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Methods Pub Date : 2025-05-21 DOI:10.1016/j.ymeth.2025.05.007

Xiaobao Chen , Yan Wu , Li Liu , Wei Wang

引用次数: 0

Abstract

The rapid advancement of RNA-based therapeutics, particularly in the wake of COVID-19 vaccine success, has prompted significant research into optimizing RNA delivery mechanisms. This study evaluates the NeoLNP^TM RNA Transfection Kit developed by Scindy Pharmaceutical, which utilizes lipid nanoparticles (LNPs) for efficient RNA encapsulation and delivery. We systematically investigate various parameters affecting transfection efficiency, including RNA concentration, RNA/LNP volume ratios, mixing techniques, LNP stability, and culture media. Our results demonstrate that the optimal RNA concentration for transfection efficiency is around 40–60 ng/µL, with a 1:0.75–1:1 RNA-to-LNP ratio yielding the highest protein expression. Additionally, we find that gentle mixing techniques outperform harsher methods, and the stability of LNP-RNA complexes significantly influences transfection outcomes. This research provides practical guidelines for enhancing RNA transfection efficiency, paving the way for more effective RNA therapeutics.

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增强RNA传递：对NeoLNPTM转染试剂的实际见解

基于RNA的治疗方法的快速发展，特别是在COVID-19疫苗成功之后，促进了对优化RNA递送机制的重要研究。本研究评估了由Scindy制药公司开发的NeoLNPTM RNA转染试剂盒，该试剂盒利用脂质纳米颗粒（LNPs）进行有效的RNA封装和传递。我们系统地研究了影响转染效率的各种参数，包括RNA浓度、RNA/LNP体积比、混合技术、LNP稳定性和培养基。我们的研究结果表明，转染效率的最佳RNA浓度约为40-60 ng/µL， RNA与lnp的比例为1:0.75-1:1，蛋白表达量最高。此外，我们发现温和混合技术优于苛刻的方法，LNP-RNA复合物的稳定性显著影响转染结果。本研究为提高RNA转染效率提供了实用指南，为开发更有效的RNA治疗方法铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods 生物-生化研究方法

CiteScore

9.80

自引率

2.10%

发文量

222

审稿时长

11.3 weeks

期刊介绍： Methods focuses on rapidly developing techniques in the experimental biological and medical sciences. Each topical issue, organized by a guest editor who is an expert in the area covered, consists solely of invited quality articles by specialist authors, many of them reviews. Issues are devoted to specific technical approaches with emphasis on clear detailed descriptions of protocols that allow them to be reproduced easily. The background information provided enables researchers to understand the principles underlying the methods; other helpful sections include comparisons of alternative methods giving the advantages and disadvantages of particular methods, guidance on avoiding potential pitfalls, and suggestions for troubleshooting.