Bioactive Carbohydrates and Dietary Fibre最新文献_第9页

Recent developments in encapsulation techniques for innovative and high-quality dairy products: Demands and challenges 创新型优质乳制品封装技术的最新发展：需求与挑战

Bioactive Carbohydrates and Dietary Fibre Pub Date : 2024-02-14 DOI: 10.1016/j.bcdf.2024.100406

Samah M. El-Sayed , Hoda S. El-Sayed , Ahmed M. Youssef

{"title":"Recent developments in encapsulation techniques for innovative and high-quality dairy products: Demands and challenges","authors":"Samah M. El-Sayed , Hoda S. El-Sayed , Ahmed M. Youssef","doi":"10.1016/j.bcdf.2024.100406","DOIUrl":"10.1016/j.bcdf.2024.100406","url":null,"abstract":"<div><p>The dairy industry is experiencing significant technological and societal changes, resulting in shifts in product availability and consumer preferences. Encapsulation technologies, such as nano/microencapsulation, are being used to increase ingredient efficiency and protect health components. Microencapsulation involves encapsulating substances in small, sealed capsules, preventing physical as well as chemical interactions, and maintaining core materials' functional, biological, and physicochemical abilities by generating a functional barrier among the wall material and core. Microencapsulation techniques like emulsification, extrusion, spray-drying, freeze-drying, coacervation, melt-extrusion, in situ polymerization, fluidized-bed coating, and supercritical fluid technology are used to encapsulate elements, vitamins, essential oils, and probiotics. The wall material selected is determined by the product's application and processing circumstances. Microencapsulation has the potential to revolutionize the manufacturing of dairy products. This review focuses on the expanding use of wall materials and diverse methods for protecting various nutraceutical components in encapsulation technology, as well as potential applications of encapsulation techniques in the dairy industry.</p></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"31 ","pages":"Article 100406"},"PeriodicalIF":0.0,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139892487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physicochemical, in vitro bile-acid binding and hypoglycaemic properties of red pitaya (Hylocereus polyrhizus) peel pectin 红番木瓜（Hylocereus polyrhizus）果皮果胶的物理化学、体外胆汁酸结合和降血糖特性

Bioactive Carbohydrates and Dietary Fibre Pub Date : 2024-02-01 DOI: 10.1016/j.bcdf.2024.100402

Majida Al-Ezzi , Kharidah Muhammad , Sri Puvanesvari Gannasin , Mohd Yusof Hamzah , Radhiah Shukri , Irene Carew

{"title":"Physicochemical, in vitro bile-acid binding and hypoglycaemic properties of red pitaya (Hylocereus polyrhizus) peel pectin","authors":"Majida Al-Ezzi , Kharidah Muhammad , Sri Puvanesvari Gannasin , Mohd Yusof Hamzah , Radhiah Shukri , Irene Carew","doi":"10.1016/j.bcdf.2024.100402","DOIUrl":"https://doi.org/10.1016/j.bcdf.2024.100402","url":null,"abstract":"<div><p>Physicochemical properties and cholesterol-lowering potential of high methoxyl pectin extracted from pitaya peel (PP) using citric acid, microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE) techniques have been reported. Enzyme-assisted citric acid extraction and its effect on the yield, physicochemical, technological, <em>in vitro</em> bile-acid binding and hypoglycaemic properties of PP pectins (PPP) were investigated and reported for the first time in this study. Pectins were extracted from whole PP using distilled water (DW) and different concentration levels of citric acid (CA) (0.5%, 1.0%, 1.5%, and 2%) with the aid of cellulase (C) enzyme. The characteristics of PPP were determined and compared to that of commercial citrus pectins (HS-R, HS-ES, and LC-S18). The PPP extract extracted with C1.5CA had the highest yield (24.63%), degree of esterification (50.20%), water-holding capacity (7.53 g water/g dry sample), oil-holding capacity (2.17 g oil/g dry sample), intrinsic viscosity, emulsifying activity (100%) and emulsion stability (100%). Good physicochemical properties of the C1.5CA-PPP extract can be associated with its notable <em>in vitro</em> bile acid-binding capacity and glucose-binding capacity. The findings indicate that the cellulase-assisted 1.5% citric acid (C1.5CA) extracted PPP has noteworthy physicochemical, hypocholesterolemic and hypoglycaemic properties. Thus, it could be a suitable alternative food ingredient for the management of high blood cholesterol and Type 2 diabetes mellitus.</p></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"31 ","pages":"Article 100402"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139699668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent developments in encapsulation techniques for innovative and high-quality dairy products: Demands and challenges 创新型优质乳制品封装技术的最新发展：需求与挑战

Bioactive Carbohydrates and Dietary Fibre Pub Date : 2024-02-01 DOI: 10.1016/j.bcdf.2024.100406

Samah M. El-Sayed, Hoda S. El‐Sayed, Ahmed M. Youssef

引用次数: 0

Chemical characterization and the bioaccessibility of bioactive compounds from seriguela (Spondias purpurea L.) pulp and by-products flours 蛇麻属植物（Spondias purpurea L.）纸浆和副产品面粉中生物活性化合物的化学特征和生物可利用性

Bioactive Carbohydrates and Dietary Fibre Pub Date : 2024-01-26 DOI: 10.1016/j.bcdf.2024.100404

Lais Brito Cangussu , Ana Letícia Rodrigues Costa , Adriana Silva Franca , Leandro Soares de Oliveira

{"title":"Chemical characterization and the bioaccessibility of bioactive compounds from seriguela (Spondias purpurea L.) pulp and by-products flours","authors":"Lais Brito Cangussu , Ana Letícia Rodrigues Costa , Adriana Silva Franca , Leandro Soares de Oliveira","doi":"10.1016/j.bcdf.2024.100404","DOIUrl":"10.1016/j.bcdf.2024.100404","url":null,"abstract":"<div><p>Seriguela (<em>Spondias purpurea</em><span><span><span> L.) is a fruit native to the semi-arid regions of Latin America. Flours prepared from Seriguela processing wastes (fibrous pulp, peels and seeds) were studied as a potential food ingredient rich in </span>bioactive compounds<span> and dietary fibers. The bioaccessibility of extractable phenolic compounds<span> was evaluated after exposure to simulated gastrointestinal conditions. Peel and seed flours presented higher dietary fiber contents than pulp flour and were deemed potential sources of carotenoids and polyphenols. However, pulp flours presented higher bioaccessibility of polyphenols than the other by-products. </span></span></span>Fourier Transform Infrared spectroscopy<span><span> (FTIR) and gas chromatography analysis indicated the presence of </span>polysaccharides<span><span> such as pectin and hemicelluloses in the analyzed flours. Furthermore, compounds not yet reported in the literature, such as trigonelline and </span>hydrolyzable tannins, were determined present in Seriguela wastes. As a contribution towards the valorization of seriguela by-products, our study demonstrated their technological potential to be applied in the formulation of processed foods.</span></span></span></p></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"31 ","pages":"Article 100404"},"PeriodicalIF":0.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139633316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of the marine red alga Laurencia papillosa extract on alloxan stimulated hyperglycemic activity in male wistar albino rats 海洋红藻乳头状月桂藻提取物对雄性 Wistar 白化大鼠阿脲刺激性高血糖活动的功效

Bioactive Carbohydrates and Dietary Fibre Pub Date : 2024-01-19 DOI: 10.1016/j.bcdf.2024.100403

Mostafa M. El-Sheekh , Naglaa R.A. Kasem , Hesham M. Alsoghier , Asmaa Jillany , Hamdy Galal , Eman A. Alwaleed

{"title":"Efficacy of the marine red alga Laurencia papillosa extract on alloxan stimulated hyperglycemic activity in male wistar albino rats","authors":"Mostafa M. El-Sheekh , Naglaa R.A. Kasem , Hesham M. Alsoghier , Asmaa Jillany , Hamdy Galal , Eman A. Alwaleed","doi":"10.1016/j.bcdf.2024.100403","DOIUrl":"10.1016/j.bcdf.2024.100403","url":null,"abstract":"<div><h3>Purpose</h3><p>Diabetes mellitus, a main health concern worldwide, is one of the most common metabolic disorders characterized by insulin secretion dysfunction.</p></div><div><h3>Materials and methods</h3><p>In this work, dietary fibers of the ethanolic extract of <em>Laurencia papillosa</em> were detected at 0.1 and 0.2 g/kg body weight using gas chromatography-mass spectrometry (GC-MS). The extract was given orally once daily to male diabetic rats induced with alloxan for two weeks. The blood cholesterol levels, complete blood count, liver function, kidney function, and antioxidant enzymes were analyzed. The molecular docking was operated using Auto Dock Vina.</p></div><div><h3>Results</h3><p>Treatment with extracts of <em>L. papillosa</em> (0.1–0.2 g/kg) induced a decrease in pre-prandial and post-prandial glucose levels. 0.1 g/kg pre-prandial extract had the highest activity to reduction glucose level (48.670 ± 0.57 mg/dL). Moreover, the complete blood count, kidney function, and liver function differed slightly in the treated mice group compared to the non-treated diabetic group. Docking analyses demonstrated that most ethanolic compounds found within <em>L. papillosa</em> extract have minimum docking scores and high binding affinity against human pancreatic alpha-amylase (PDB: <span>4W93</span><svg><path></path></svg>), insulin receptor (PDB:<span>1IR3</span><svg><path></path></svg>), and SIRT-6 (PDB: <span>3k35</span><svg><path></path></svg>)</p></div><div><h3>Conclusion</h3><p>Results further suggested that the concentration of 0.1 g/kg of <em>L. papillosa</em> extract was more effective than 0.2 g/kg in reducing glucose levels. Our study induced that in vivo and in silico results indicated that <em>L. papillosa</em> extract has been a viable natural bioactive source and might be a great potential source for future antidiabetic medicine.</p></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"31 ","pages":"Article 100403"},"PeriodicalIF":0.0,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139639583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the functional properties of ethanolic and polysaccharide extracts of Spirulina platensis 评估螺旋藻乙醇提取物和多糖提取物的功能特性

Bioactive Carbohydrates and Dietary Fibre Pub Date : 2024-01-05 DOI: 10.1016/j.bcdf.2024.100401

Reza Habibi , Mehrnoosh Tadayoni , Hossein Mohammadpour

{"title":"Evaluation of the functional properties of ethanolic and polysaccharide extracts of Spirulina platensis","authors":"Reza Habibi , Mehrnoosh Tadayoni , Hossein Mohammadpour","doi":"10.1016/j.bcdf.2024.100401","DOIUrl":"10.1016/j.bcdf.2024.100401","url":null,"abstract":"<div><p><span>This study aimed to investigate the prebiotic<span>, antioxidant and antimicrobial activity of ethanolic and polysaccharide extracts from </span></span><span><em>Spirulina platensis</em></span>. The ethanol extract was obtained by the soxhlet method and the polysaccharide was extracted with hot water. Total phenol content was obtained by the folin-ciocalteu method. The ethanol extract and the polysaccharide extracted from the <em>S. platensis</em> had a high phenol. Also, the extract had comparable prebiotic properties with commercial prebiotic (Inulin). Both extracts in the period of 24–72 h affected the growth and viability of the <span><em>Lactobacillus</em><em> plantarum</em></span><span> A7. Based on the results, the half-maximal inhibitory concentration (IC50) of DPPH free radical for ethanol extract and polysaccharide was estimated to be 0.279 and 0.319 mg/mL. Also, the IC50 of hydroxyl radical scavenging capacity for polysaccharide and ethanol extract was calculated to be 0.167, 0.14 mg/mL, respectively. Therefore, these compounds can be introduced as natural substances with antioxidant, prebiotic and antimicrobial capabilities in the food and pharmaceutical industries.</span></p></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"31 ","pages":"Article 100401"},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139394750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sulfated polysaccharides from the viscera of Mustelus shark: Characterization and antioxidant, anticoagulant and anti-proliferative activities 鼬鲨内脏中的硫酸化多糖：特性及抗氧化、抗凝血和抗增殖活性

Bioactive Carbohydrates and Dietary Fibre Pub Date : 2024-01-01 DOI: 10.1016/j.bcdf.2023.100399

Ola Abdelhedi , Mourad Jridi , Hanen Najjaa , Nacim Zouari , Hichem Sebai , Moncef Nasri

{"title":"Sulfated polysaccharides from the viscera of Mustelus shark: Characterization and antioxidant, anticoagulant and anti-proliferative activities","authors":"Ola Abdelhedi , Mourad Jridi , Hanen Najjaa , Nacim Zouari , Hichem Sebai , Moncef Nasri","doi":"10.1016/j.bcdf.2023.100399","DOIUrl":"https://doi.org/10.1016/j.bcdf.2023.100399","url":null,"abstract":"<div><p><span><span>In the present study, sulfated polysaccharides (SPs) from smooth hound shark were extracted using different precipitation agents, the cetylpyridinium chloride (CPC) or ethanol, named SHSP-I and SHSP-II, respectively. The UV–visible scan demonstrated the absence of </span>nucleic acids<span><span> or proteins in the extracted SHSPs. The antioxidant, anticoagulant and anti-proliferative activities were also investigated. Results showed that SHSPs displayed reducing and scavenging capacities, as shown by the oxygen radical absorbance capacity, the total </span>antioxidant capacity<span><span> and the hydroxyl radical-scavenging activities. Moreover, extracted SPs were able to prolong the activated partial thromboplastin time<span> (aPTT), the prothrombin time (PT) and the </span></span>thrombin time (TT) and they reduced the </span></span></span>fibrinogen<span><span> level (FL), without inducing erythrocytes hemolysis, which indicated that they inhibited the intrinsic and extrinsic coagulation pathways and the thrombin-mediated fibrin formation. The SHSP-I was more effective on prolonging the </span>blood clotting time, by more than 120 s at 1 mg/ml in the aPTT and about 70 s and 60 s at 2.5 mg/ml in the PT and TT, respectively, compared to the SHSP-II. The anti-proliferative activity of SHSPs was tested against the growth of K562 human myelogenous leukemia cells and Caco-2 colon cancer cells. Data showed that both polysaccharides have a dose-dependent inhibitory effect, where SHSP-I exhibited the strongest activity against the tested cells and the K562 was more sensitive than Caco-2 line cells to the action of SHSPs. The overall data suggested that SPs from shark viscera could be useful as natural nutraceutical agents.</span></p></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"31 ","pages":"Article 100399"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139107984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The wound healing effect and DNA damage repair of exopolysaccharide extracted from Weissella cibaria MED17 从西伯利亚魏氏菌MED17中提取的外多糖的伤口愈合效果和DNA损伤修复作用

Bioactive Carbohydrates and Dietary Fibre Pub Date : 2023-12-31 DOI: 10.1016/j.bcdf.2023.100400

Özge Kahraman-Ilıkkan , Selin Akad Dinçer , Elif Şeyma Bağdat , Işılsu Çağlar , Enes Dertli , Hümeyra İspirli , Feride İffet Şahin

{"title":"The wound healing effect and DNA damage repair of exopolysaccharide extracted from Weissella cibaria MED17","authors":"Özge Kahraman-Ilıkkan , Selin Akad Dinçer , Elif Şeyma Bağdat , Işılsu Çağlar , Enes Dertli , Hümeyra İspirli , Feride İffet Şahin","doi":"10.1016/j.bcdf.2023.100400","DOIUrl":"https://doi.org/10.1016/j.bcdf.2023.100400","url":null,"abstract":"<div><p><span>Lactic acid bacteria can synthesize exopolysaccharides (EPSs), and some of these EPSs have pharmaceutical or medicinal properties. However, the wound healing capacities of EPSs have rarely been reported. In this study, EPS extracted from </span><span><em>Weissella</em><em> cibaria</em></span><span><span><span> MED17 was used to investigate wound healing capacity. MED17 EPS was previously characterized and antioxidant capacity<span> was also demonstrated. The application dose of the compound was determined by the MTT assay. The </span></span>comet assay was applied to reveal the free radical scavenging activity and protection capacity of MED 17 against DNA damage by inducing </span>oxidative stress and DNA damage with Mitomycin-C (MMC) in the cells. The comet assay showed that 15 mg/ml MED 17 EPS could ameliorate DNA damage by 50%. Moreover, the scratch test investigated the wound-healing effect of MED 17 EPS on HT-29 colon cells. MED 17 EPS could stimulate the migration of HT-29 and ameliorate MMC-induced DNA damage. However, further studies need to be conducted to clarify the mechanisms of the wound-healing process of MED 17.</span></p></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"31 ","pages":"Article 100400"},"PeriodicalIF":0.0,"publicationDate":"2023-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139100020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of apricot, guar and locust bean gum hydrocolloids on pasting, antioxidant, rheology, thermal and sensory properties of gluten-free breads 杏胶、瓜尔豆胶和槐豆胶水胶体对无麸质面包的糊化、抗氧化、流变、热和感官特性的影响

Bioactive Carbohydrates and Dietary Fibre Pub Date : 2023-12-26 DOI: 10.1016/j.bcdf.2023.100397

Afshan Mumtaz Hamdani , Idrees Ahmed Wani , Naseer Ahmad Bhat , Khushnuma Maqbool , Shabir Ahmad Mir

{"title":"Effect of apricot, guar and locust bean gum hydrocolloids on pasting, antioxidant, rheology, thermal and sensory properties of gluten-free breads","authors":"Afshan Mumtaz Hamdani , Idrees Ahmed Wani , Naseer Ahmad Bhat , Khushnuma Maqbool , Shabir Ahmad Mir","doi":"10.1016/j.bcdf.2023.100397","DOIUrl":"https://doi.org/10.1016/j.bcdf.2023.100397","url":null,"abstract":"<div><p><span><span>Gluten-free breads were prepared from rice-chickpea composite flour. Apricot, guar and locust bean gum samples were added at 2% (w/w) test concentration to dough samples. The moisture, protein, fat, ash and carbohydrate contents of bread formulations were in the range of 41.00–42.34, 14.61–15.14, 2.03–2.70, 0.50–0.67 and 40.00–41.03 g/100 g, respectively. Addition of gums increased the peak, trough, breakdown, final and setback viscosities of dough formulations except for apricot gum where a decrease from 1348.0 to 1089 cP (peak viscosity), 1203.0–989.0 cP (trough viscosity), 145.0–100 cP (breakdown viscosity), 2276.0–1760.0 cP (final viscosity) and 1073.0–771.0 cP (setback viscosity) was observed. </span>Antioxidant activity of dough formulations increased from 10.61 to 16.83% (DPPH radical scavenging) and 1.13–1.98 AAE/g (reducing power) with the incorporation of gums. Hunter Colour </span><em>‘L’</em><span> value decreased from 91.89 to 88.39 and 72.83 to 68.36 in dough formulations and bread crumb, respectively, while it increased from 57.56 to 60.96 in bread crust, with the incorporation of gums. Significant increase and decrease was observed in gelatinization temperatures of flours and breads, respectively, upon incorporation of gums. Texture and sensory attributes of breads with incorporated gums were improved.</span></p></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"31 ","pages":"Article 100397"},"PeriodicalIF":0.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139100018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sulfated polysaccharide from brown algae Dictyota caribaea stimulates macrophages to an antitumoral phenotype 褐藻中的硫酸化多糖可刺激巨噬细胞形成抗肿瘤表型

Bioactive Carbohydrates and Dietary Fibre Pub Date : 2023-12-23 DOI: 10.1016/j.bcdf.2023.100396

Alexia Nathália Brígido Assef , Felipe Barros Teles , Leonardo Paes Cinelli , Diego Veras Wilke

{"title":"Sulfated polysaccharide from brown algae Dictyota caribaea stimulates macrophages to an antitumoral phenotype","authors":"Alexia Nathália Brígido Assef , Felipe Barros Teles , Leonardo Paes Cinelli , Diego Veras Wilke","doi":"10.1016/j.bcdf.2023.100396","DOIUrl":"https://doi.org/10.1016/j.bcdf.2023.100396","url":null,"abstract":"<div><p><span><span>Fucoidans are sulfated </span>polysaccharides<span> capable of exerting biological activities such as antitumor and immunomodulatory effects. Previous studies demonstrated the antitumor activity of non-cytotoxic fucoidans from the seaweed </span></span><em>Dictyota caribaea</em> (Dc-SP) <em>in vivo</em><span>. Macrophages (Mfs) are innate immune cells capable of promoting or inhibiting tumor growth depending on the stimulus. This study aimed to evaluate the immunostimulant activity of Dc-SP on a murine Mfs cell line (RAW 264.7) </span><em>in vitro</em><span>. Dc-SP was assessed for its ability to modify cell viability<span><span> and stimulate the production of antitumor markers on RAW 264.7 cells. Dc-SP induced an increase (p < 0.05) in the production of NO and cytokines TNF-α, IL-1β, and IL-10. The exposure of RAW 264.7 to Dc-SP also increased (p < 0.05) the expression of M1 phenotype markers such as iNOS, </span>CD86<span>, and MHC II. Dc-SP did not exhibit direct cytotoxicity on a murine melanoma cell line (B16–F10). However, the conditioned medium (CM) obtained from RAW 264.7 previously stimulated with Dc-SP (CM-Dc-SP) showed antiproliferative activity on tumor cells. B16–F10 incubated with CM-Dc-SP showed a cytostatic profile, tumor cells did not alter membrane integrity, however, they suffered morphological changes such as cell shrinkage and high granularity. In conclusion, Dc-SP stimulated RAW 264.7 to an antitumor phenotype.</span></span></span></p></div>","PeriodicalId":38299,"journal":{"name":"Bioactive Carbohydrates and Dietary Fibre","volume":"31 ","pages":"Article 100396"},"PeriodicalIF":0.0,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139100019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0