The wound healing effect and DNA damage repair of exopolysaccharide extracted from Weissella cibaria MED17

IF 3.6
Özge Kahraman-Ilıkkan , Selin Akad Dinçer , Elif Şeyma Bağdat , Işılsu Çağlar , Enes Dertli , Hümeyra İspirli , Feride İffet Şahin
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引用次数: 0

Abstract

Lactic acid bacteria can synthesize exopolysaccharides (EPSs), and some of these EPSs have pharmaceutical or medicinal properties. However, the wound healing capacities of EPSs have rarely been reported. In this study, EPS extracted from Weissella cibaria MED17 was used to investigate wound healing capacity. MED17 EPS was previously characterized and antioxidant capacity was also demonstrated. The application dose of the compound was determined by the MTT assay. The comet assay was applied to reveal the free radical scavenging activity and protection capacity of MED 17 against DNA damage by inducing oxidative stress and DNA damage with Mitomycin-C (MMC) in the cells. The comet assay showed that 15 mg/ml MED 17 EPS could ameliorate DNA damage by 50%. Moreover, the scratch test investigated the wound-healing effect of MED 17 EPS on HT-29 colon cells. MED 17 EPS could stimulate the migration of HT-29 and ameliorate MMC-induced DNA damage. However, further studies need to be conducted to clarify the mechanisms of the wound-healing process of MED 17.

从西伯利亚魏氏菌MED17中提取的外多糖的伤口愈合效果和DNA损伤修复作用
乳酸菌可以合成外多糖(EPSs),其中一些 EPSs 具有制药或药用特性。然而,EPSs 的伤口愈合能力却鲜有报道。在本研究中,研究人员利用从西伯利亚魏氏菌 MED17 中提取的 EPS 来研究伤口愈合能力。MED17 EPS 的特征和抗氧化能力也已得到证实。通过 MTT 试验确定了化合物的应用剂量。彗星试验是通过使用丝裂霉素-C(MMC)诱导细胞氧化应激和 DNA 损伤来揭示 MED17 清除自由基的活性和保护 DNA 免受损伤的能力。彗星试验表明,15 毫克/毫升的 MED 17 EPS 可使 DNA 损伤减轻 50%。此外,划痕试验研究了 MED 17 EPS 对 HT-29 结肠细胞的伤口愈合作用。MED 17 EPS 能刺激 HT-29 的迁移,并能改善 MMC 引起的 DNA 损伤。不过,MED 17 的伤口愈合机制还需要进一步研究。
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来源期刊
Bioactive Carbohydrates and Dietary Fibre
Bioactive Carbohydrates and Dietary Fibre Agricultural and Biological Sciences-Food Science
CiteScore
6.00
自引率
0.00%
发文量
38
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