{"title":"The Effect of Modified Media on The Antibacterial Activity of The Sea Sponge Symbion Fungi, Fusarium solani","authors":"Muslihuddin Aini","doi":"10.29303/jbt.v23i3.5281","DOIUrl":"https://doi.org/10.29303/jbt.v23i3.5281","url":null,"abstract":"The purpose of this study was to analyze the biomass production and bioactivity of sponge symbiont mushroom extracts on modified media using experimental laboratory methods. Biomass was calculated by weighing the wet weight of the symbiont mushroom mycelium and then tested for antibacterial activity with an extract concentration of 500 µg/disk. The results of this study, namely the weight of F.solani mycelium on MKD + MGV media was 10 grams, the mass of mycelium on MKD media was 9 grams, while the mass of mycelium on MEB standard media was 9.7 grams. The yield weight of the F.solani crude extract cultured on MKD + MGV media was 0.0386 grams or 0.3860% of the wet weight, on MKD media it showed that the crude extract weight was 0.0276 grams or 0.3067% of the wet weight, while on MEB the crude extract weight was 0.0119 grams or 0.1190% of the wet weight. The results of the antibacterial test showed that the largest inhibition zone for MDR (Meticilin Resistant Staphylococcus aureus) bacteria was F.solani crude extract cultured on MKD + MGV media, namely 25.83 ± 3.37 mm, F.solani cultured on MKD media of 22.10 ± 2.51 mm , while F. solani cultured on MEB media was 19.00 ± 0.52 mm. The results of the mass culture of the fungus Fusarium solani on modified media showed that the mushrooms cultured on MKD + MGV media had greater biomass, crude extract and antibacterial activity compared to MKD and MEB culture media.","PeriodicalId":382423,"journal":{"name":"Jurnal Biologi Tropis","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130651127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vera Septaria, Musliar Kasim, I. Suliansyah, A. Syarif, J. Juniarti
{"title":"Exploration and Characterization of Grain Morphology and Rice (caryopsis) Genotypes of Local Rice in Solok Regency, West Sumatra","authors":"Vera Septaria, Musliar Kasim, I. Suliansyah, A. Syarif, J. Juniarti","doi":"10.29303/jbt.v23i3.5010","DOIUrl":"https://doi.org/10.29303/jbt.v23i3.5010","url":null,"abstract":"South Solok Regency is one of the potential areas for rice development in West Sumatra Province. The local rice in South Solok Regency is a type of rice that can potentially be developed for germplasm conservation. One genetic resource that needs to be developed is abiotic and biotic resistance to conserve germplasm so that the local rice plants in South Solok can be maintained on certain lands. The first step that must be taken in conservation efforts is to find, identify and collect local rice genotypes in South Solok Regency. This study aimed to explore and characterize the morphology of local grain and rice in Solok Selatan Regency. This study used a survey method, and the exploration location was determined by purposive sampling. The survey results found 18 local South Solok rice genotypes from four sub-districts. The results of grain morphological characterization showed differences in quantitative properties (length, width, thickness, and weight of 100 grains of grain and caryopsis and grain tail length) as well as differences in qualitative characteristics (lemma and palea color, rice color (caryopsis) and shape, rice (caryopsis). Dendrogram results from cluster analysis of 18 local rice genotypes of South Solok, the coefficient of similarity was between 0.27 – 0.64 (27 -64%) with the highest similarity coefficient of genotypes G013 (rambuman variety) and G016 (redek sangir variety) with a value of 0.64 (64%).","PeriodicalId":382423,"journal":{"name":"Jurnal Biologi Tropis","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127916006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nur Hikmah, I. Budiarsa, F. Dhafir, M. Bialangi, Masrianih, M. Trianto
{"title":"Characteristics Morphology and Morphometry Lepidotrigona terminata (Hymenoptera: Meliponini) in Central Sulawesi","authors":"Nur Hikmah, I. Budiarsa, F. Dhafir, M. Bialangi, Masrianih, M. Trianto","doi":"10.29303/jbt.v23i3.5005","DOIUrl":"https://doi.org/10.29303/jbt.v23i3.5005","url":null,"abstract":"Stingless bees are eusocial bees belonging to the Apidae family and the Meliponini subfamily. This study aims to describe the morphological and morphometric and nest structure characteristics of Stingless bee Lepidotrigona terminata from Parigi Moutong Regency. This study used the roaming method with a purposive sampling technique. Obtained morphological and morphometry data were analyzed using the Principal Component Analysis (PCA) method with PAST4 software. The morphological characters of Lepidotrigona terminata had a dominant black body, on the thorax, black scutum and scutellum covered with brown hair and yellow packaging on the edges. The tegula is brown, the tibia on the legs is black, the hind tibia is slightly hairy (plumose), antennae with 11 flagellomeres, the number of hamuli is 8. The most dominant character in the formation of seven groups of stingless bees in this study were Hamuli Number (HN), Fore Wing Length (FWL), and Length of Forewing Including Tegula (WL1). There are differences in character size compared to the same or different species in other areas.","PeriodicalId":382423,"journal":{"name":"Jurnal Biologi Tropis","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128868110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. A. Akbar, Khairunnisa Khairunnisa, Mardiah Mardiah, E. Pandia
{"title":"The Effect of Fermentation Time on The Organoleptic Test of Kombucha Tea","authors":"M. A. Akbar, Khairunnisa Khairunnisa, Mardiah Mardiah, E. Pandia","doi":"10.29303/jbt.v23i3.5096","DOIUrl":"https://doi.org/10.29303/jbt.v23i3.5096","url":null,"abstract":"Kombucha is a fermented product of sweet tea solution which is added with kombucha starter and scoby. Kombucha culture is a combination of bacteria and yeast called SCOBY (Symbiotic Culture of Bacteria and Yeast). This activity aims to determine the effect of time on the organoleptic test in making kombucha tea. This study used quantitative methods and data collection techniques by conducting organoleptic tests on respondents, in this study using a completely randomized design (CRD). Organoleptic consisting of taste, aroma and color. The results showed that on average all respondents preferred kombucha tea on the 15th day of fermentation. The organoleptic test conducted on 30 respondents had a preference level with the highest percentage for each parameter consisting of 60% taste, 70% aroma and 70% color. Where the longer the fermentation time, the level of preference of the testers for the organoleptic response increases.","PeriodicalId":382423,"journal":{"name":"Jurnal Biologi Tropis","volume":"85 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121738429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phytoplankton as Bioindikator of Water Quality in The Batu Dendeng River West Lombok","authors":"Afiatul Hafifah, Agil Al Idrus, Lalu Japa","doi":"10.29303/jbt.v23i3.5407","DOIUrl":"https://doi.org/10.29303/jbt.v23i3.5407","url":null,"abstract":"This exploratory descriptive study with a qualitative approach was aims to analyze the water quality of the Batu Dendeng River in West Lombok through a study of the phytoplankton community structure. Sampling was carried out using purposive sampling method. Water sampling was carried out using plankton net of 20µ mesh sized. Observation and identification of phytoplankton was carried out at the Biology Laboratory of FKIP University of Mataram using a binocular microscope. Phytoplankton data was analyzed based on abundance, species diversity, species uniformity and species dominance indexs. The results showed that the community structure of identified phytoplankton in the Batu Dendeng River consisted of 5 classes and 52 species with abundance value of 714.074 (ind/L). Phytoplankton species diversity, uniformity, and dominance indexs of Batu Dendeng River were 2.591, 0.66, and 0.13 respectively. The water pollution criteria based on the diversity index of phytoplankton species, indicates the water quality of the Batu Dendeng River West Lombok was in the slightly polluted category.","PeriodicalId":382423,"journal":{"name":"Jurnal Biologi Tropis","volume":"260 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132327649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad Yuda Putratama, Rafdinal, Dwi Gusmalawati, Program Studi Rafdinal, Fakultas Biologi, Matematika dan, Ilmu Pengetahuan, Alam
{"title":"Utilization of Plants in Pangari Activities by The Dayak Ribun Tribe Community in Suka Gerundi Village, Parindu District Sanggau Regency","authors":"Muhammad Yuda Putratama, Rafdinal, Dwi Gusmalawati, Program Studi Rafdinal, Fakultas Biologi, Matematika dan, Ilmu Pengetahuan, Alam","doi":"10.29303/jbt.v23i3.5227","DOIUrl":"https://doi.org/10.29303/jbt.v23i3.5227","url":null,"abstract":"Pangari activity is a mutual cooperation activity in farming carried out by the Dayak people in West Kalimantan. However, there is no scientific information that examines the use of plants in pangari activities by the Dayak Ribun people in Suka Gerundi Village, Parindu District, Sanggau Regency. Therefore, this research was carried out with the aim of finding out how the pangari activities were carried out by the Dayak Ribun people, identifying the types of plants, the proportions of plant organ parts, and the benefits of the forms of the types and plant organs used. This research was conducted for 6 months, from March to September 2022. The method used in this study was the snowball sampling method with 15 respondents. The results showed that the pangari activities carried out took place at the time of planting and harvesting rice. 58 species from 32 plant families were used. The highest Citation Frequency value is rice (Oryza sativa L.) at 100%. The most commonly found plant families are Arecaceae, Moraceae, Poaceae, and Zingiberaceae. The percentage of plant parts that are widely used are leaves (31%). Forms of utilization of plants, namely, as building materials, crafts, medicines and food. The highest Informant Agreement Ratio (IAR) value is a form of utilization as food with a value of 0.8156.","PeriodicalId":382423,"journal":{"name":"Jurnal Biologi Tropis","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130799256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Community Structure of Phytoplankton in The Bongkok River of Kubu Raya Regency, West Kalimantan","authors":"Armawati Armawati, E. Daningsih, Wolly Candramila","doi":"10.29303/jbt.v23i3.5238","DOIUrl":"https://doi.org/10.29303/jbt.v23i3.5238","url":null,"abstract":"Located around the Mangrove Production and Protection Forest in Tanjung Harapan Village, Kubu Raya Regency, the Bongkok River quality needs to be studied to determine the quality of the river. This study aims to determine the physicochemical quality of water and the community structure of phytoplankton in the Bongkok River during the dry and rainy seasons. The survey method was used at three stations and 9 observation points in both seasons. Phytoplankton types are identified up to the species level. The physicochemical factors observed included temperature, transparency, water flow, pH, salinity, dissolved oxygen, dissolved carbon dioxide, and levels of nitrate and phosphate. The community structure is seen from the abundance, diversity, uniformity, and dominance index values. From a physicochemical perspective, the Bongkok River is a brackish water type with relatively low levels of phosphate and nitrate nutrients and high dissolved CO2, but dissolved oxygen is still good enough to support aquatic life. There are 41 phytoplankton species from 6 classes, including Bacillariophyceae, Chlorophyceae, Cyanophyceae, Dinophyceae, Euglenaphyceae, and Xanthophyceae. The phytoplankton abundance in the dry season is higher than in the rainy season but is still at a low to moderate level. The phytoplankton diversity in the Bongkok River is in the medium category (2.46 – 2.67) but has a high evenness (0.72-0.89) and low dominance (0.11-0.18). Overall, the water conditions in the Bongkok River are sufficient to support the life of aquatic biota. However, the abundance of phytoplankton is still at a low to moderate level, especially during the rainy season.","PeriodicalId":382423,"journal":{"name":"Jurnal Biologi Tropis","volume":"83 12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128175843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Diversity and Dominance of Sea Urchins (Echinoidea) on Sambalagi Beach, Siompu Island, South Buton Regency","authors":"Maretik Maretik, Ramad Arya Fitra, Winardi Winardi","doi":"10.29303/jbt.v23i3.5338","DOIUrl":"https://doi.org/10.29303/jbt.v23i3.5338","url":null,"abstract":"Many species of sea urchins (Echinoidea) can be found in the waters of Sembalagi Beach, Siompu Island. However, the diversity of sea urchins (Echinoidea) on the coast has not been scientifically researched and published. This study aims to determine the types, hunting, poaching and dominance of sea urchins (Echinoidea) at Sambalagi Beach, Siompu Island, South Buton Regency. This study uses a quantitative descriptive method with observation techniques. There are three research stations, namely station I (rocky and seagrass substrate), station II (sandy and seagrass substrate), and station III (rocky and seagrass substrate). The data obtained were analyzed using the formulation of documents, documents, secrecy and Shannon-Winner domination. The results showed that there were 5 species of sea urchins (Echinoidea) which namely Diadema setosum, Echinothrix calamaris, Tripneustes gratilla, Echinometra mathaei and Salmacis sphaeroides. The species diversity index (H') for the three abnormalities was classified as moderate, namely station I (H' 1,34), station II (H' 1,18) and station III (H' 1,22). The highest relative abundance of Echinoidea was found in Tripneustes gratilla species at Station II (37,67%) which had a lot and the lowest relative decline index was found in Echinothrix calamaris and Echinometra mathaei at Station III (1,52%) less abundant. The highest evenness index is at station II (0,85) which is very even and the lowest is at station III (0,76) which is more evenly distributed. And the highest dominance index is at station II (0,32) with moderate dominance and the lowest dominance index is at station I (0,29) with low dominance.","PeriodicalId":382423,"journal":{"name":"Jurnal Biologi Tropis","volume":"353 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134450274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mosquito Repellent Bioactivity Combination Extract Of Nutmeg Leaf (Myrisctica fragrans Houtt), Lemongrass (Cymbopogon nardus L.) and Apis dorsata Binghami Nests","authors":"Meisa Tabita Rogahang, O. Naharia, Y. Mokosuli","doi":"10.29303/jbt.v23i3.5037","DOIUrl":"https://doi.org/10.29303/jbt.v23i3.5037","url":null,"abstract":"Nutmeg leaf, lemongrass, and bee nests contain bioactive compounds that have potential as mosquito repellents. The purpose of this study was to learn about the bioactivity of a combination extract of nutmeg leaf, lemongrass, and bee nests, as well as to determine the efficient mosquito repellent lotion recipe from a combination of nutmeg leaf, lemongrass, and bee nests extract. The maceration process was used for extraction. Total flavonoid content was estimated by comparing it to the flavonoid quercetin. The absorbance value was measured with a UV-Vis spectrophotometer. The concentration of the extract combination in the lotion used was 0%, 2.5%, 5% and 10%. The total flavonoid concentration was found to be 16.67 mgQE/g. The 2.5% formula lotion has a protective power of 22%, the 5% formula lotion has a power of 62.96%, and the 10% formula lotion has a power of 82.41%. From these results, it can be seen that a formula with a concentration of nutmeg leaf, lemongrass, and bee nests as much as 10% can be the most effective mosquito repellent. The outcomes of this study are expected to help the community select environmentally friendly and safe insect repellents.","PeriodicalId":382423,"journal":{"name":"Jurnal Biologi Tropis","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133829534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antibacterial Activity of Pineapple Peel Eco-enzyme (Ananas comosus L.) on Growth Pseudomonas aeruginosa and Staphylococcus epidermidis","authors":"H. Hendri, Zulfa Zakiah, R. Kurniatuhadi","doi":"10.29303/jbt.v23i3.5272","DOIUrl":"https://doi.org/10.29303/jbt.v23i3.5272","url":null,"abstract":"Eco-enzyme is a fermented product from an organic waste substrate, molasses, and water. Eco-enzyme can be used as antibacterial because it produces organic compounds such as alcohol and acetic acid. Pineapple peel waste (Ananas comosus L.) can be used as an eco-enzyme. This study aimed to test the antibacterial activity of pineapple peel eco enzyme against Pseudomonas aeruginosa and Staphylococcus epidermidis. The study used the method of determining the Minimum Inhibitory Concentration (MIC) and Minimum Killing Concentration (MBC) of pineapple peel eco-enzyme with 8 treatment levels namely negative control (aquadest), positive control (ciprofloxacin 5 mg/mL), 3.125%, 6.25 %, 12.5%, 25%, 50%, and 100% eco-enzymes. Based on the research results, it was known that the MIC value of pineapple peel eco-enzyme against P. aeruginosa, and S. epidermidis was 12.5% and 3.125%, respectively. Eco- enzymes from pineapple peel did not show minimum killing concentrations. Eco-enzyme of pineapple peel is only bacteriostatic towards the growth of P. aeruginosa and S. epidermidis bacteria.","PeriodicalId":382423,"journal":{"name":"Jurnal Biologi Tropis","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133724559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}