Antibacterial Activity of Pineapple Peel Eco-enzyme (Ananas comosus L.) on Growth Pseudomonas aeruginosa and Staphylococcus epidermidis

H. Hendri, Zulfa Zakiah, R. Kurniatuhadi
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Abstract

Eco-enzyme is a fermented product from an organic waste substrate, molasses, and water. Eco-enzyme can be used as antibacterial because it produces organic compounds such as alcohol and acetic acid. Pineapple peel waste (Ananas comosus L.) can be used as an eco-enzyme. This study aimed to test the antibacterial activity of pineapple peel eco enzyme against Pseudomonas aeruginosa and Staphylococcus epidermidis. The study used the method of determining the Minimum Inhibitory Concentration (MIC) and Minimum Killing Concentration (MBC) of pineapple peel eco-enzyme with 8 treatment levels namely negative control (aquadest), positive control (ciprofloxacin 5 mg/mL), 3.125%, 6.25 %, 12.5%, 25%, 50%, and 100% eco-enzymes. Based on the research results, it was known that the MIC value of pineapple peel eco-enzyme against P. aeruginosa, and S. epidermidis was 12.5% and 3.125%, respectively. Eco- enzymes from pineapple peel did not show minimum killing concentrations. Eco-enzyme of pineapple peel is only bacteriostatic towards the growth of P. aeruginosa and S. epidermidis bacteria.
菠萝皮生态酶对铜绿假单胞菌和表皮葡萄球菌的抑菌活性研究
生态酶是由有机废物基质、糖蜜和水发酵而成的产物。生态酶可以用于抗菌,因为它产生有机化合物,如酒精和乙酸。菠萝皮废弃物(Ananas comosus L.)可作为一种生态酶。研究了菠萝皮生态酶对铜绿假单胞菌和表皮葡萄球菌的抑菌活性。采用阴性对照(aquadest)、阳性对照(环丙沙星5 mg/mL)、3.125%、6.25%、12.5%、25%、50%和100%生态酶8种处理水平,测定菠萝皮生态酶的最小抑制浓度(MIC)和最小杀伤浓度(MBC)。根据研究结果可知,菠萝皮生态酶对铜绿假单胞菌和表皮葡萄球菌的MIC值分别为12.5%和3.125%。从菠萝皮中提取的生物酶没有显示出最低杀伤浓度。菠萝皮生态酶仅对铜绿假单胞菌和表皮葡萄球菌有抑菌作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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