Journal of the Taiwan Institute of Chemical Engineers最新文献

{"title":"Mechanistic insights into the impact of sinter ore basicity on softening and melting behavior","authors":"Wen-chien Tsai ,&nbsp;Yu-ning Chiu ,&nbsp;Kai-chun Chang ,&nbsp;Jia-shyan Shiau ,&nbsp;Ke-miao Lu ,&nbsp;Tsung-yen Huang ,&nbsp;Shan-wen Du ,&nbsp;Ker-chang Hsieh ,&nbsp;Hao-long Chen ,&nbsp;Shih-kang Lin","doi":"10.1016/j.jtice.2025.106536","DOIUrl":"10.1016/j.jtice.2025.106536","url":null,"abstract":"<div><h3>Background</h3><div>Traditional empirical indices, such as T₁₀ (softening onset) and T₄₀ (softening completion), are widely used to evaluate how sinter basicity (B₂) influences the softening and melting (S&amp;M) behavior in blast furnace ironmaking. Although simple and intuitive, these indices show weak correlation with the underlying physicochemical mechanisms and microstructural evolution.</div></div><div><h3>Methods</h3><div>This study employs a systematic analytical framework integrating a dynamically controlled atmosphere, microstructural characterization, exhaust gas analysis, and thermodynamic modeling. By comparing sinter ore with different B₂, we evaluate the limitations of empirical indices and provide a mechanistic understanding of B₂ effects on S&amp;M behavior.</div></div><div><h3>Significant Finding</h3><div>Comparing typical (B₂ = 1.93) and high-basicity sinter (B₂ = 2.36), we find that increased B₂ lowers the initial FeO liquefaction temperature, promoting earlier, more abundant liquid formation. This accelerates the direct reduction (FeO + C → Fe + CO) and results in greater shrinkage within the 1100−1250 °C range. Extensive early liquid formation fills pore spaces, intensifying softening at lower temperatures. Additionally, high-B₂ sinter lowers the slag dripping temperature (1399 °C vs. 1456 °C), facilitating early FeO liquid drainage and suppressing FeO coarsening in 1330–1430 °C range. These findings indicate that increasing sinter basicity enhances S&amp;M behavior, improves gas permeability, and optimizes reduction reactions in blast furnace operations.</div></div>","PeriodicalId":381,"journal":{"name":"Journal of the Taiwan Institute of Chemical Engineers","volume":"181 ","pages":"Article 106536"},"PeriodicalIF":6.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145615096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of transition metals (Co, Ni, Fe) on methanol selective catalytic oxidation performance of CeMOy mixed oxide catalysts","authors":"Junhao Jing ,&nbsp;Zhitao Han ,&nbsp;Liangzheng Lin ,&nbsp;Sihan Yin ,&nbsp;Tingjun Liu ,&nbsp;Chuanqiu Gao ,&nbsp;You Tian ,&nbsp;Dong Ma","doi":"10.1016/j.jtice.2025.106565","DOIUrl":"10.1016/j.jtice.2025.106565","url":null,"abstract":"<div><h3>Background</h3><div>Methanol selective catalytic oxidation (CH₃OH-SCO) is an effective technology for CH₃OH removal. However, the development of catalysts with excellent stability, cost-effectiveness, and high activity at low temperatures remains a major challenge in reducing unburned methanol emissions from methanol-fueled engines.</div></div><div><h3>Methods</h3><div>In this study, CeMO<em>_y</em> (<em>M</em> = Co, Ni, Fe) mixed oxide catalysts were synthesized via the sol-gel method and characterized by XRD, XPS, EPR, H₂-TPR, O₂-TPD, CO₂-TPD, and in-situ DRIFTS to investigate the influence of transition metals on CH₃OH-SCO performance.</div></div><div><h3>Significant findings</h3><div>Catalytic activity tests demonstrated that the CeCoO<em>_y</em> catalyst exhibited the best performance, achieving 90 % methanol conversion at 173 °C and maintaining complete conversion without detectable byproducts over a broad temperature range (200–450 °C). Furthermore, the CeCoO<em>_y</em> catalyst showed excellent stability and sulfur resistance. Characterization analyses revealed that its superior catalytic performance was attributed to the enhanced interfacial effect between CeO₂ and Co₃O₄. In-situ DRIFTS analysis confirmed that methanol oxidation followed the Mars-van Krevelen (MvK) mechanism. This study clarified the role of different transition metals in modulating oxygen vacancies, oxygen species, and basic sites in CeMO<em>_y</em> catalysts, providing guidance for the design of efficient and low-cost mixed oxide catalysts.</div></div>","PeriodicalId":381,"journal":{"name":"Journal of the Taiwan Institute of Chemical Engineers","volume":"181 ","pages":"Article 106565"},"PeriodicalIF":6.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Covalent bridging strategy with multiscale silicon architectures for polyurethane composites: Enhanced interfacial compatibility and long-term durability","authors":"Qian Chen ,&nbsp;Lili Guo ,&nbsp;Shihui Chen ,&nbsp;Xiaoyun Li ,&nbsp;Junwei Wang","doi":"10.1016/j.jtice.2025.106531","DOIUrl":"10.1016/j.jtice.2025.106531","url":null,"abstract":"<div><h3>Background</h3><div>Polyurethane (PU) composites face critical limitations in industrial applications due to weak interfacial adhesion and polarity mismatch between conventional fillers and polymer matrices. This study proposes a multiscale interface engineering approach to mitigate these limitations.</div></div><div><h3>Methods</h3><div>The silica (SiO₂) particle-modified graphene oxide (SKG) via silane coupling agent was prepared, and the corresponding composites were fabricated by incorporating various SKG ratios (0-5 wt‰) into PU through melt blending. The SAXS analysis was conducted to characterize the microphase separation, and mechanical tests were utilized, especially aging measurements were carried out to explore the durability performance.</div></div><div><h3>Significant findings</h3><div>The incorporation of SKG at extremely low loading level led to a remarkable improvement in mechanical properties, with tensile strength increasing by 37.1% from 25.3 MPa to 34.7 MPa, alongside a high tear resistance of 78.4 kN/m. More importantly, aging tests demonstrated outstanding durability, retaining over 80% of the original tensile strength even under simulated 48-month service conditions. This strategy establishes a versatile and scalable pathway for developing high-performance elastomers that combine strength with long-term durability in concise way, showing promise for shock-absorbing applications.</div></div>","PeriodicalId":381,"journal":{"name":"Journal of the Taiwan Institute of Chemical Engineers","volume":"181 ","pages":"Article 106531"},"PeriodicalIF":6.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precise regulation of structural properties in manganese ferrite nanocatalysts for enhanced ozone decomposition","authors":"Xiaotong Li ,&nbsp;Yitong Fu ,&nbsp;Zhuxu Li ,&nbsp;Guoqing Rui ,&nbsp;Lei Zhong ,&nbsp;Zhen Li ,&nbsp;Wei Liu ,&nbsp;Songjian Zhao","doi":"10.1016/j.jtice.2025.106540","DOIUrl":"10.1016/j.jtice.2025.106540","url":null,"abstract":"<div><h3>Background</h3><div>Manganese ferrite (MnFe₂O₄) is a promising alternative to precious metal catalyst, yet its application in ozone decomposition (deO₃) remains underexplored.</div></div><div><h3>Methods</h3><div>Herein, a series of MnFe₂O₄ nanocatalysts with different structural properties were precisely designed.</div></div><div><h3>Significant findings</h3><div>The optimal MnFe₂O₄ prepared via alkaline co-precipitation method (abbreviated as MFO-AP) catalyst, exhibits superior ozone decomposition performance, which can be attributed to its unique amorphous structure, nanoscale characteristics, enhanced redox properties, abundant oxygen vacancies, and Lewis acid sites. In particular, Lewis acid sites promote ozone activation by coordinating with O₃ oxygen atoms, enhancing ozone decomposition. At a weight hourly space velocities (WHSV) of 600 L·g⁻¹·h⁻¹, the decomposition rate of 50 ppm ozone in dry air reaches 99%, and there is still a decomposition efficiency of 98% after 10 h. Furthermore, under 60% relative humidity (RH), the atomic oxygen from ozone dissociation preferentially occupies the Mn unsaturated sites due to confinement and spatial separation, enhancing ozone decomposition efficiency, while water molecules preferentially bind to the Fe unsaturated sites, improving moisture resistance.</div></div>","PeriodicalId":381,"journal":{"name":"Journal of the Taiwan Institute of Chemical Engineers","volume":"181 ","pages":"Article 106540"},"PeriodicalIF":6.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145615091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emission reduction characteristics of N2O from low COD/N ratio sewage treated with different potential biocathodes","authors":"Yuling Fu ,&nbsp;Jing Tian ,&nbsp;Yucheng Zhang ,&nbsp;Qingyun Wang ,&nbsp;Jingran Zhang ,&nbsp;Huimin Zhang ,&nbsp;Chuqiao Wang ,&nbsp;Shan Huang","doi":"10.1016/j.jtice.2025.106534","DOIUrl":"10.1016/j.jtice.2025.106534","url":null,"abstract":"<div><h3>Background</h3><div>Nitrous oxide (N₂O) is a byproduct of biological nitrogen removal and a potent greenhouse gas. While biocathodes enhance denitrification performance, systematic studies on their N₂O emission characteristics remain limited. Furthermore, the majority of urban sewage has a low chemical oxygen demand/nitrogen (COD/N) ratio (&lt; 5) that requires additional carbon sources, producing secondary pollution and undermining the low-carbon treatment strategy.</div></div><div><h3>Methods</h3><div>In this study, a denitrifying biocathode system was constructed to investigate N₂O emissions under varying COD/N ratios (3, 2, and 1) and applied electric potentials (−0.4 V to 0.0 V).</div></div><div><h3>Significant findings</h3><div>The results demonstrated that N₂O emission factors (N₂O_ef) in the experimental group ranged from 0.51 % (−0.4 V) to 2.44 % (0.0 V), significantly lower than the control group (3.36 %). N₂O_ef decreased by 79.10 % at −0.4 V compared with 0.0 V, attributable to enhanced electron supply and complete denitrification. Under varying COD/N ratios, the N₂O_ef values in the experimental group (1.17–1.57 %) were approximately half those in the control (2.29–4.80 %), confirming the dominant role of applied potential compared with the COD/N ratio. High-throughput sequencing revealed enrichment in N₂O-reducing genera (<em>Truepera</em> and <em>Ignavibacterium</em>) under low potentials, while PICRUSt2 predicted the upregulation of key functional genes such as <em>narGHI, nirK, nirS, norC</em>, and <em>nosZ</em>, particularly <em>nosZ</em> (9.7-fold increase at −0.4 V). In addition, this study proposed a strategy for N₂O abatement using biocathodes, providing theoretical support for their application in mitigating N₂O emissions.</div></div>","PeriodicalId":381,"journal":{"name":"Journal of the Taiwan Institute of Chemical Engineers","volume":"181 ","pages":"Article 106534"},"PeriodicalIF":6.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145615098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adsorption mechanism of polyepoxysuccinic acid on the sphalerite surface and its effect on flotation separation","authors":"Jiaxi Jin,&nbsp;Hao Lai,&nbsp;Yuyang Xiao,&nbsp;Xuemei Lu,&nbsp;Peilun Shen,&nbsp;Jinpeng Cai,&nbsp;Xiao Wei,&nbsp;Dianwen Liu","doi":"10.1016/j.jtice.2025.106518","DOIUrl":"10.1016/j.jtice.2025.106518","url":null,"abstract":"<div><h3>Background</h3><div>This study elucidated the adsorption mechanism of polyepoxysuccinic acid (PESA) on sphalerite (ZnS) surfaces and its consequential effect on the flotation-based separation of sphalerite from chalcopyrite.</div></div><div><h3>Methods</h3><div>Micro-flotation experiments, contact angle measurements, adsorption behavior studies, and zeta potential analysis were conducted. Density Functional Theory (DFT) calculations, X-ray Photoelectron Spectroscopy (XPS), and Time-of-Flight Secondary Ion Mass Spectrometry (ToF-SIMS) were employed to investigate the interaction mechanism.</div></div><div><h3>Findings</h3><div>PESA selectively depressed sphalerite, reducing its recovery to &lt;4 % at pH 7 while chalcopyrite recovery remained above 91 %, facilitating efficient separation. PESA enhanced sphalerite hydrophilicity, decreasing the contact angle from 56.32° to 22.67°. Adsorption and zeta potential analyses demonstrated competitive adsorption between PESA and SBX on sphalerite, but preferential SBX adsorption on chalcopyrite. DFT revealed strong carboxyl–Zn coordination (−74.86 kJ/mol) at bridge sites, corroborated by XPS shifts in Zn 2p and enrichment of O–C<img>O, as well as ToF-SIMS detection of PESA–Zn fragments. These findings establish PESA as an environmentally benign, selective depressant, enabling sustainable Cu–Zn separation under low-alkali conditions.</div></div>","PeriodicalId":381,"journal":{"name":"Journal of the Taiwan Institute of Chemical Engineers","volume":"181 ","pages":"Article 106518"},"PeriodicalIF":6.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145464901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dual strategy of hydroxylation modified Si-O-C bond cooperation and π-π stacking: Efficient photocatalytic degradation of tetracycline","authors":"Yunjing Li ,&nbsp;Yangrui Xu ,&nbsp;Jie Chen ,&nbsp;Yuchen Wang ,&nbsp;Yu Cheng ,&nbsp;Liguang Tang ,&nbsp;Hongping Li ,&nbsp;Jie Jin ,&nbsp;Xinlin Liu ,&nbsp;Ziyang Lu","doi":"10.1016/j.jtice.2025.106523","DOIUrl":"10.1016/j.jtice.2025.106523","url":null,"abstract":"<div><h3>Background</h3><div>Tetracycline (TC) a common antibiotic. However, its residues in the environment can cause water pollution, posing potential threats to the ecological environment and human health.</div></div><div><h3>Methods</h3><div>In this study, a novel BSM-PDI photocatalyst was hydrothermally synthesized by modifying perylene diimide (PDI) with bagasse-derived material (BSM) through hydrochloric acid-mediated strategy.</div></div><div><h3>Significant Findings</h3><div>During synthesis, surface hydroxyl groups on BSM condensed with PDI carboxyl groups, forming Si-O-C covalent bonds that enhanced stability and transformed PDI stacking from disordered J-aggregates to planar H-aggregates, strengthening π-π conjugation. Furthermore, BSM hydroxyls promoted TC adsorption via hydrogen bonding. The modified BSM-PDI demonstrated strengthened visible light absorption capacity, enhanced charge separation and transfer performance, and a more negative LUMO level, facilitating oxygen reduction to ·O₂^- for TC degradation. Under visible light, BSM-PDI achieved 62.37% TC degradation within 10 min, representing a 5.07-fold and 3.41-fold increase over unmodified PDI and N-PDI, respectively. In this study, biomass waste was used to develop new photocatalytic materials, which provided a new idea for the efficient elimination of TC residues in water.</div></div>","PeriodicalId":381,"journal":{"name":"Journal of the Taiwan Institute of Chemical Engineers","volume":"181 ","pages":"Article 106523"},"PeriodicalIF":6.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145518314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reactive Green 19–anchored poly(hexamethylene biguanide) on regenerated cellulose nanofiber membranes for durable, reusable antibacterial activity against E. coli","authors":"Thi My Huong Dinh ,&nbsp;Quang-Vinh Le ,&nbsp;Yi-Hua Gao ,&nbsp;Bing-Lan Liu ,&nbsp;Anusorn Seubsai ,&nbsp;Chen-Yaw Chiu ,&nbsp;Chi-Yun Wang ,&nbsp;Shen-Long Tsai ,&nbsp;Kuei-Hsiang Chen ,&nbsp;Yu-Kaung Chang","doi":"10.1016/j.jtice.2025.106522","DOIUrl":"10.1016/j.jtice.2025.106522","url":null,"abstract":"<div><h3>Background</h3><div>Regenerated cellulose (RC) nanofiber membranes are hydrophilic and renewable yet rarely provide durable, low-leaching antibacterial activity. We use Reactive Green 19 (RG19) as a chemical anchor to immobilize polyhexamethylene biguanide (PHMB) on RC, thereby creating a contact-active surface for capturing and killing <em>Escherichia coli</em> without biocide release.</div></div><div><h3>Methods</h3><div>Cellulose acetate was hydrolyzed to RC to increase surface –OH density; RG19 was grafted to introduce sulfonate (–SO₃⁻) sites; PHMB was immobilized via electrostatic/ionic interactions. SEM, FTIR, and thermal analysis verified functionalization. Antibacterial performance was evaluated using time-kill (0–120 min) and antibacterial efficiency (<em>AE</em>, %). Durability (5 cycles), PHMB leaching (72 h in saline), PHMB loading, and cytocompatibility were evaluated.</div></div><div><h3>Significant findings</h3><div>Optimized RC–RG19–PHMB membranes achieved complete <em>E. coli</em> inactivation within 120 min with ∼100 % <em>AE</em>. PHMB immobilization capacity was 0.69 mg/g; full inhibition occurred with 0.373 mg/g. Membranes maintained full antibacterial activity over five reuse cycles and showed 8.39 % PHMB leaching after 72 h in saline, consistent with strong RG19–PHMB–RC interactions and contact-active behavior. Cytocompatibility remained high (≥ 100 % relative viability). Overall, RG19-anchored PHMB on RC yields a sustainable, reusable, non-leaching antibacterial platform applicable to wound dressings, protective fabrics, antimicrobial coatings, and water purification.</div></div>","PeriodicalId":381,"journal":{"name":"Journal of the Taiwan Institute of Chemical Engineers","volume":"181 ","pages":"Article 106522"},"PeriodicalIF":6.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145518247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cationic cellulose aerogel immobilized with horseradish peroxidase for visible light-assisted degradation and detoxification of Crystal Violet dye","authors":"Hayam A. Alwabsi","doi":"10.1016/j.jtice.2025.106526","DOIUrl":"10.1016/j.jtice.2025.106526","url":null,"abstract":"<div><h3>Background</h3><div>The persistent release of toxic dyes such as crystal violet (CV) into aquatic environments poses significant ecological and health risks due to their chemical stability, toxicity, and resistance to biodegradation.</div></div><div><h3>Methodology</h3><div>In this study, a novel bio-catalytic platform based on horseradish peroxidase (HRP) immobilized on a cationic cellulose-based aerogel (CATCE aerogel) was developed for the efficient and sustainable degradation of CV under visible light irradiation. Firstly, microcrystalline cellulose was cationized using 3‑chloro-2-hydroxypropyl) trimethylammonium chloride for the formation of cationic cellulose (CATCE). Secondly, CATCE-HRP aerogel was formed by mixing HRP with CATCE and followed by a lyophilization process. The finding illustrated that the CATCE aerogel exhibited a porous structure, and biocompatible matrix that enhanced enzyme stability, reusability, and catalytic efficiency.</div></div><div><h3>Significant findings</h3><div>The immobilized HRP exhibited superior thermal and pH stability, maintaining 79.4% activity after 28 days of storage and 58.4% activity after ten reuse cycles, significantly outperforming free HRP enzyme. Complete CV degradation (100%) was achieved within 90 min under optimized conditions (aerogel dose 0.5 g/L, CV concentration 5 ppm, pH 6, temperature 45 °C). Kinetic studies confirmed pseudo-first-order reaction behavior (<em>k</em> = 0.03021 min⁻¹, R² = 0.9283). Response Surface Methodology (RSM) further optimized the opertating parameters, predicting 80.06% degradation at 17.5 ppm CV under mild operational conditions. The CATCE-HRP aerogel demonstrated high operational stability, retaining 74.5% of its catalytic activity after five successive reuse cycles. Importantly, the Microtox® bioassay revealed a dramatic reduction in acute toxicity post-treatment, converting the effluent from extremely toxic to non-toxic and biocompatible status. These findings underscore the potential of CATCE-HRP aerogel as a robust, eco-friendly, and scalable solution for advanced wastewater treatment, contributing a novel strategy for mitigating dye pollution in industrial effluents.</div></div>","PeriodicalId":381,"journal":{"name":"Journal of the Taiwan Institute of Chemical Engineers","volume":"181 ","pages":"Article 106526"},"PeriodicalIF":6.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered miniature CRISPR activation system promotes dodecanedioic acid production in Candida viswanathii","authors":"Nam Ngoc Pham ,&nbsp;Chih-Hsuan Chien ,&nbsp;Chin-Wei Chang,&nbsp;Yu-Chen Hu","doi":"10.1016/j.jtice.2025.106548","DOIUrl":"10.1016/j.jtice.2025.106548","url":null,"abstract":"<div><h3>Background</h3><div>Dodecanedioic acid (DDA) is a valuable monomer with broad industrial applications. The yeast <em>Candida viswanathii</em> can convert dodecane into 12-carbon DDA, making it an attractive host for metabolic engineering toward sustainable production. A recently developed miniature dCasMINI protein, when fused with the VPR activator, enables efficient CRISPR activation (CRISPRa) in mammalian cells, thereby simplifying gene cloning and advancing gene therapy applications. However, its potential for metabolic engineering in yeast has yet to be explored.</div></div><div><h3>Methods</h3><div>We reconstituted the dCasMINI system in <em>C. viswanathii</em> and optimized it by modifying nuclear localization signals, sgRNA design, promoters and transcriptional activators. The redesigned system was applied to activate various endogenous genes. The impact of activating different transporter genes on DDA production was evaluated.</div></div><div><h3>Significant findings</h3><div>The native dCasMINI-VPR system impaired <em>C. viswanathii</em> growth and failed to induce gene expression. By tuning design elements and replacing VPR with another yeast activator (Med2), we established a new dCasMINI-Med2 system that enabled robust activation of both constitutive and inducible genes while minimizing growth defects. Using this system, activation of transporter genes STL1_4 and HST6 enhanced DDA titers by 13–16 %. This study repurposes dCasMINI-based CRISPRa in <em>C. viswanathii</em> for metabolic engineering. The optimized dCasMINI-Med2 platform expands the CRISPRa toolbox for yeasts and demonstrates that transporter regulation can alleviate metabolic bottlenecks and improve DDA biosynthesis.</div></div>","PeriodicalId":381,"journal":{"name":"Journal of the Taiwan Institute of Chemical Engineers","volume":"181 ","pages":"Article 106548"},"PeriodicalIF":6.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145681061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}