New Microbes and New Infections最新文献

{"title":"Unveiling the presence of ESBL-producing coliform bacteria in the aquaculture system of Cumilla District of Bangladesh","authors":"Rakibul Islam ,&nbsp;Nazia Afrin ,&nbsp;Md Shakhawate Hossain","doi":"10.1016/j.nmni.2026.101706","DOIUrl":"10.1016/j.nmni.2026.101706","url":null,"abstract":"<div><div>The increasing use of antibiotics in aquaculture has raised global concerns about the emergence and spread of antimicrobial resistance (AMR) in aquatic environments.</div></div><div><h3>Objectives</h3><div>This study investigated the presence of extended-spectrum β-lactamase (ESBL) producing coliform bacteria and their antibiotic resistance profiles in aquaculture systems across six Upazilas of the Cumilla district of Bangladesh.</div></div><div><h3>Methods</h3><div>Water quality parameters (pH, dissolved oxygen, temperature, and ammonia) were measured with HACH kits. Bacterial isolates were screening through culture dependent methods and biochemical tests. Antibiotic susceptibilities were tested by disc diffusion method and resistant genes were identified using qPCR. Isolates ESBL were confirmed by double disc synergy test (DDST).</div></div><div><h3>Results</h3><div>Microbiological analysis revealed significant variation in the bacterial contamination across sites. The most dominant bacteria were <em>Escherichia coli</em>, <em>Klebsiella</em> spp., <em>Enterobacter</em> spp., and <em>Salmonella</em> spp. All isolates were resistant to multiple antibiotics and multi-drug resistance index (MDRI) values exceeded the threshold 0.2. Isolates showed higher resistance against β-lactam antibiotics. Phenotypic ESBL testing identified 34.3 % of isolates as ESBL producers. The qPCR analysis confirmed the presence of multiple antibiotic resistance genes (ARGs), including bla SHV, bla TEM, bla CMY, bla CTX-M15, Sul1, Sul2, and tetracycline resistance genes, along with mobile genetic elements (MGEs) such as TSO.</div></div><div><h3>Conclusion</h3><div>The findings suggest that aquaculture environments in the Cumilla District may serve as a significant reservoir of multi-drug resistant and ESBL-producing coliform bacteria. Responsible antibiotic practices and regular surveillance are necessary to limit the spread of AMR in the aquaculture industry of Bangladesh.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101706"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146025443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multidrug-resistant Klebsiella pneumoniae in a pediatric ICU: Emergence of newly identified ST7120 and the first report of ST792 in Taiwan","authors":"Yu-Chin Chen ,&nbsp;Cheng-Yen Kao ,&nbsp;Chi-Chung Chen ,&nbsp;Hung-Jen Tang ,&nbsp;Tu-Hsuan Chang","doi":"10.1016/j.nmni.2026.101721","DOIUrl":"10.1016/j.nmni.2026.101721","url":null,"abstract":"<div><h3>Background</h3><div>Multidrug-resistant (MDR) <em>Klebsiella pneumoniae</em> outbreaks in pediatric intensive care units (PICUs) pose serious risks to vulnerable patients. We report a one-year cluster of MDR <em>K. pneumoniae</em> in a PICU, along with the genetic relatedness, antimicrobial resistance profiles, and resistance mechanisms of the isolates.</div></div><div><h3>Methods</h3><div>We investigated a one-year cluster of MDR <em>K. pneumoniae</em> in a 14-bed PICU from September 2021 to August 2022. Selected clinical isolates underwent pulsed-field gel electrophoresis, multilocus sequence typing, and resistance gene detection. Patient location and transfer records were reviewed to infer possible transmission pathways.</div></div><div><h3>Results</h3><div>Twenty <em>K. pneumoniae</em> isolates from 12 patients, including 10 preterm infants, were obtained and classified into six sequence types. Two predominant sequence types (STs) were identified: ST792 (CTX-M-14, SHV-12), not previously reported in Taiwan, and the newly identified ST7120 (CTX-M-15, SHV-11, TEM-1). The cluster exhibited two temporal peaks, driven by different STs. During the second peak, up to three STs circulated simultaneously. One patient carried both ST792 and ST7120, suggesting coinfection or sequential acquisition. Three invasive infections occurred, two with carbapenem-resistant ST7120 (including one fatal case) and one with extended-spectrum β-lactamase-producing ST792, which had severe neurologic sequelae.</div></div><div><h3>Conclusion</h3><div>This MDR <em>K. pneumoniae</em> cluster involved a newly identified ST, ST7120, and the first reported case of ST792 in Taiwan, both were associated with prolonged colonization and invasive disease. Molecular typing revealed complex transmission routes not detectable through phenotypic methods alone. Integrating molecular epidemiology into routine MDRO surveillance could improve early outbreak detection and containment.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101721"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146174243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary estimation of the basic reproductive number of yellow fever in Colombia, 2024–2026","authors":"Alfonso J. Rodriguez-Morales ,&nbsp;Hernán Vargas","doi":"10.1016/j.nmni.2026.101724","DOIUrl":"10.1016/j.nmni.2026.101724","url":null,"abstract":"","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101724"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146174246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Staphylococcus pettenkoferi: first report from blood culture in Rio de Janeiro, Brazil","authors":"Bruna Ribeiro Sued-Karam ,&nbsp;Julianna Giordano Botelho Olivella ,&nbsp;Ana Paula D'Alincourt Carvalho-Assef ,&nbsp;Ana Luiza Mattos-Guaraldi ,&nbsp;Paula Marcele Afonso Pereira Ribeiro","doi":"10.1016/j.nmni.2026.101697","DOIUrl":"10.1016/j.nmni.2026.101697","url":null,"abstract":"","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101697"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145982115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Persistent signals from MERS-CoV in 2025: surveillance gaps, clinical heterogeneity, and preparedness priorities","authors":"Jaffar A. Al-Tawfiq ,&nbsp;Abdullah M. Assiri","doi":"10.1016/j.nmni.2026.101704","DOIUrl":"10.1016/j.nmni.2026.101704","url":null,"abstract":"","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101704"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146025438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing health care delivery by adapting diagnostics in a low-resource setting: The case of San Miguel Hospital, Sucumbíos, Ecuador","authors":"Willemijn Johanna Catharina van Keizerswaard ,&nbsp;Jacob van der Ende ,&nbsp;Carolien Maria Bouwman ,&nbsp;Maria Vanessa Dávila Campos ,&nbsp;Martin Peter Grobusch","doi":"10.1016/j.nmni.2025.101696","DOIUrl":"10.1016/j.nmni.2025.101696","url":null,"abstract":"<div><h3>Background</h3><div>To strategically optimize diagnostic capacity in a low-resource, rural hospital setting, we developed a systematic evaluation of diagnostic tool needs and associated costs. This local data-driven method, accounting for patient characteristics and disease prevalence, can be adapted to other contexts.</div></div><div><h3>Methods</h3><div>A retrospective patient record analysis was conducted at San Miguel Hospital (SMH) in Sucumbíos, Ecuador, which provides outpatient and emergency care to inhabitants of the Ecuadorian and Colombian Amazon basin. Ethics approval was granted retrospectively by the Research Ethics Committee on Human Beings of the Universidad San Francisco de Quito.</div><div>Data was retrieved from electronic medical records (EMRs) of the first 796 patients seen after hospital opening. For each of the 1975 diagnoses made, patient characteristics and the presence or absence of appropriate diagnostic tools were recorded. Unavailable tools were further evaluated for accessibility within the local context.</div></div><div><h3>Results</h3><div>Serving a population primarily of mixed and indigenous ethnicities, SMH confirmed 66 % of diagnoses using existing resources, with potassium hydroxide (KOH) fungal microscopy, chikungunya and influenza rapid tests, and access to anatomical pathology identified as the diagnostic tools offering the highest return on investment.</div></div><div><h3>Conclusions</h3><div>Data from SMH's EMRs suggest which diagnostic tools would offer the greatest return on investment through increased diagnostic confirmation. This evaluation tool supports improved health care delivery at SMH and, with adaptation, can be applied in comparable health care settings.</div></div><div><h3>Trial registration</h3><div>N/A.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101696"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146025440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial potential of silver nanoparticles synthesized from mushroom extracts against drug-resistant pathogens","authors":"Mehrajud Din Talie ,&nbsp;Nusrat Ahmad,&nbsp;Mansoor Ahmad Malik ,&nbsp;Abdul Hamid Wani,&nbsp;Mohd Yaqub Bhat","doi":"10.1016/j.nmni.2026.101716","DOIUrl":"10.1016/j.nmni.2026.101716","url":null,"abstract":"<div><div>Green synthesis of silver nanoparticles (AgNPs) has emerged as a sustainable alternative to conventional chemical methods due to the presence of natural bioactive compounds in biological systems. Although Ascomycetous macrofungi are rich sources of antibacterial and antioxidant metabolites, their potential in nanoparticle synthesis remains largely unexplored. In the present study, silver nanoparticles were synthesized for the first time using extracts of the Ascomycetous mushrooms <em>Geopora sumneriana</em> and <em>Verpa bohemica</em> as eco-friendly reducing and capping agents. The biosynthesized AgNPs were characterized using UV–visible spectroscopy, field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. UV–visible analysis confirmed nanoparticle formation through a characteristic surface plasmon resonance band at ∼420–450 nm, while FESEM revealed predominantly spherical to hexagonal nanoparticles. XRD analysis confirmed the crystalline nature of AgNPs with a face-centered cubic structure, and FTIR results indicated the involvement of fungal biomolecules such as proteins, phenolics, and polysaccharides in nanoparticle reduction and stabilization. The biosynthesized AgNPs were subsequently evaluated for their antimycotic and antibacterial efficacy, including minimum inhibitory concentration (MIC) determination, against a range of phytopathogenic fungi and clinically relevant bacterial strains. The AgNPs exhibited strong antifungal and antibacterial activities against <em>Aspergillus niger</em>, <em>Penicillium chrysogenum</em>, <em>Pythium ultimum</em>, <em>Escherichia coli</em>, <em>Salmonella gallinarum</em>, and <em>Staphylococcus aureus</em>. The MIC values of AgNPs synthesized using <em>Verpa bohemica</em> ranged from 0.9 to 1.0 mg/mL for fungal strains and 0.7–0.8 mg/mL for bacterial strains. Similarly, AgNPs derived from <em>Geopora sumneriana</em> showed MIC values ranging from 0.6 to 1.1 mg/mL for fungi and 0.4–0.8 mg/mL for bacteria. The pronounced growth inhibition of diverse pathogenic microorganisms underscores the strong antimicrobial potential of these mycosynthesized AgNPs. Overall, the study highlights <em>Geopora sumneriana</em> and <em>Verpa bohemica</em> as efficient biogenic nanofactories and demonstrates the potential of mycosynthesized AgNPs as eco-friendly nanofungicides and nanobactericides for controlling multidrug-resistant pathogens in agriculture, aquaculture, and horticulture.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101716"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146174157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yellow fever among captive non-human primates in La Paz, Bolivia, 2025","authors":"Juan Pablo Escalera-Antezana ,&nbsp;Jorge Luis Aviles-Sarmiento ,&nbsp;Claudia Marcela Montenegro-Narvaez ,&nbsp;Hugo Antonio Castro-Calderon ,&nbsp;Jorge Luis Bonilla-Aldana ,&nbsp;D. Katterine Bonilla-Aldana ,&nbsp;Alfonso J. Rodriguez-Morales","doi":"10.1016/j.nmni.2025.101695","DOIUrl":"10.1016/j.nmni.2025.101695","url":null,"abstract":"","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"69 ","pages":"Article 101695"},"PeriodicalIF":5.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145939196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First-ever imported case of clade Ib Mpox in Senegal","authors":"Martin Faye ,&nbsp;Abdourahmane Sow ,&nbsp;Mamadou Aliou Barry ,&nbsp;Papa samba Dieye ,&nbsp;Boly Diop ,&nbsp;Ndeye Codou Sall ,&nbsp;Boubacar Diallo ,&nbsp;Serigne Fallou MBacké Ngom ,&nbsp;Aminata Massaly ,&nbsp;Fatou Diène Thiaw ,&nbsp;Viviane Marie Pierre Cisse ,&nbsp;Landry Gérard Boussiengui ,&nbsp;Daye Ka ,&nbsp;Ousmane Faye ,&nbsp;NDongo Dia ,&nbsp;Moussa Seydi ,&nbsp;El Hadj Mamadou Ndiaye ,&nbsp;Ibrahima Socé Fall","doi":"10.1016/j.nmni.2026.101700","DOIUrl":"10.1016/j.nmni.2026.101700","url":null,"abstract":"","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"69 ","pages":"Article 101700"},"PeriodicalIF":5.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145977893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A letter in response to the article in new microbes and new infections entitled “Shortage of praziquantel in Europe: a clarion call for urgent action”","authors":"Johannes Waltz,&nbsp;Harald Nusser","doi":"10.1016/j.nmni.2025.101683","DOIUrl":"10.1016/j.nmni.2025.101683","url":null,"abstract":"","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"69 ","pages":"Article 101683"},"PeriodicalIF":5.4,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}