New Microbes and New Infections最新文献

{"title":"Targeted next-generation sequencing enhances precision and rapid detection in healthcare-associated infection Surveillance: Unveiling multidrug-resistant colonization in ICUs","authors":"Tangchun Liu ,&nbsp;Shuyan Deng ,&nbsp;Wandi Liu,&nbsp;Jinzhao Zhang,&nbsp;Pengfei Wang ,&nbsp;Zhengfei Yang","doi":"10.1016/j.nmni.2025.101589","DOIUrl":"10.1016/j.nmni.2025.101589","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aims to evaluate the potential advantages of targeted next-generation sequencing (tNGS) over conventional bacterial culture methods for pathogen detection in hospital-associated infections (HAIs).</div></div><div><h3>Methods</h3><div>All EICU medical staff and all medical staff from the Physical Examination Centre completed a questionnaire. Nasopharyngeal specimens were collected from medical staff who met all of the inclusion criteria and none of the exclusion criteria. EICU medical staff provided 2 samples each, while Physical Examination Centre staff provided 1 sample each. For EICU medical staff, one of their two nasopharyngeal swabs was subjected to tNGS testing, and the other to bacterial culture testing. For the PEC staff, their nasopharyngeal swabs were subjected to tNGS testing. Additionally, six pairs of spectacles and six keyboards used by EICU medical staff were randomly selected, and the surfaces were swabbed with sterile swabs for tNGS testing.</div></div><div><h3>Results</h3><div>In 23 nasal swab samples from EICU group, tNGS detected 14 species of microorganism in 29 instances within 19 h. Bacterial culture detected 2 species of microorganism in 4 instances, 2 positive samples within 19 h and confirmed another 2 positive samples within 69 h. A total of 42 samples with 14 different microorganism species were collected from the nasopharyngeal swabs of 23 EICU members and 15 PEC members. Among them, 29 cases (69 %) of 14 different microorganisms were detected in EICU staff, with an average of 1.3 microorganism species detected per person, while 13 cases (28 %) of 6 different microorganisms were detected in PEC staff, with an average of 0.9 microorganism species detected per person. The most common colonizing bacteria included <em>Staphylococcus aureus</em>, <em>Acinetobacter baumannii</em>, and <em>Klebsiella</em> spp. Compared to bacterial culture, tNGS offers advantages in monitoring HAIs, including a broad range of detectable microorganisms, high sensitivity of results, and shorter reporting time for positive results. Bacteria colonizing the EICU carry more antibiotic resistance genes.</div></div><div><h3>Conclusions</h3><div>tNGS outperforms conventional culture in healthcare-associated infection surveillance, with higher sensitivity and accelerated pathogen identification. Simultaneously, tNGS revealed extensive colonization of multidrug-resistant (MDR) pathogens (e.g., <em>Acinetobacter baumannii</em>, MRSA) in EICU environments, highlighting its utility in monitoring complex antimicrobial resistance patterns.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101589"},"PeriodicalIF":2.9,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143887316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and antimicrobial resistance of gram-positive pathogens in Lebanon: The need for surveillance and stewardship","authors":"Yara Khachab ,&nbsp;Racha Khoumassi ,&nbsp;Elie Salem Sokhn","doi":"10.1016/j.nmni.2025.101588","DOIUrl":"10.1016/j.nmni.2025.101588","url":null,"abstract":"<div><h3>Background</h3><div>Resistance in Gram-positive organisms, including methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) and vancomycin-resistant <em>Enterococcus</em> (VRE), poses a significant healthcare challenge globally. However, data on these organisms in Lebanon remain limited. This retrospective study aimed to assess the prevalence and antimicrobial resistance patterns of <em>Staphylococcus aureus</em> (<em>S. aureus</em>), coagulase-negative <em>Staphylococci</em> (CoNS), and <em>Enterococcus</em> spp. in clinical infections at the Lebanese Hospital Geitaoui – UMC from 2017 to 2023.</div></div><div><h3>Methods</h3><div>A total of 2676 isolates were collected from urine, blood, respiratory specimens, and other infection sites. Bacterial identification was performed following WHO clinical bacteriology procedures, utilizing gram staining, catalase and coagulase tests, and biochemical assays. Antimicrobial susceptibility testing was conducted using the Kirby-Bauer disk diffusion method and minimum inhibitory concentration (MIC) analysis, interpreted according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Statistical analyses were performed using SPSS® version 24, with significance set at p &lt; 0.05.</div></div><div><h3>Results</h3><div>CoNS were the most prevalent (42.83 %), followed by <em>Enterococcus</em> spp. (28.81 %) and <em>S. aureus</em> (28.36 %). Blood cultures had the highest isolation rates (29.04 %), predominantly CoNS (76.45 %). <em>Enterococcus</em> spp. dominated urinary tract infections (85.01 %), while <em>S. aureus</em> was prevalent in wound/surgical site infections (59.23 %). Gender-specific trends showed CoNS and <em>S. aureus</em> more in males, while <em>Enterococcus</em> spp. infections were more common in females.</div></div><div><h3>Conclusion</h3><div>This study provides valuable insights into the prevalence and resistance patterns of Gram-positive pathogens in a Lebanese hospital setting. The findings highlight the need for continuous surveillance and stringent antibiotic stewardship to combat antimicrobial resistance effectively.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101588"},"PeriodicalIF":2.9,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arboviral infections and pregnancy: An overlooked public health challenge","authors":"Ibrahim Nagmeldin Hassan","doi":"10.1016/j.nmni.2025.101587","DOIUrl":"10.1016/j.nmni.2025.101587","url":null,"abstract":"","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101587"},"PeriodicalIF":2.9,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota influences Plasmodium falciparum malaria susceptibility","authors":"Aly Kodio ,&nbsp;Drissa Coulibaly ,&nbsp;Safiatou Doumbo ,&nbsp;Salimata Konaté ,&nbsp;Abdoulaye Kassoum Koné ,&nbsp;Souleymane Dama ,&nbsp;Amadou Niangaly ,&nbsp;Mamadou Lamine Tall ,&nbsp;Ahmed Mohamed Konaté ,&nbsp;Coralie L'Ollivier ,&nbsp;A. Levasseur ,&nbsp;Fadi Bittar ,&nbsp;Abdoulaye Djimdé ,&nbsp;Ogobara K. Doumbo ,&nbsp;Didier Raoult ,&nbsp;Mahamadou Ali Thera ,&nbsp;Stéphane Ranque","doi":"10.1016/j.nmni.2025.101586","DOIUrl":"10.1016/j.nmni.2025.101586","url":null,"abstract":"<div><h3>Background</h3><div>The gut microbiota has recently been associated with malaria susceptibility/resistance in animal models and humans. This study aimed to assess its influence on malaria attack and <em>Plasmodium</em> parasitemia in children living in a malaria-endemic area of Mali.</div></div><div><h3>Methods</h3><div>Healthy children were enrolled in a 16-month cohort study in Bandiagara. Their gut bacteria and fungi community structures were characterized via 16S and ITS metabarcoding at enrolment. Clinicians monitored malaria attacks. Asymptomatic <em>Plasmodium</em> carriage was assessed by real-time polymerase chain reaction.</div></div><div><h3>Results</h3><div>Of the 300 children, 107 (36 %) had at least one malaria attack, and 82 (27 %) had at least one episode of asymptomatic <em>Plasmodium</em> parasitemia. The gut bacterial community structure, but not the fungal community, was associated with susceptibility/resistance to both malaria attacks and asymptomatic <em>P. falciparum</em> parasitemia. Higher gut bacteria richness was independently associated with susceptibility to both asymptomatic parasitemia episodes and malaria attacks. 17 bacteria, and 7 fungi were associated with susceptibility to malaria attacks, and 8 bacteria, and 3 fungi were associated with resistance. 15 bacteria and 13 fungi were associated with susceptibility to asymptomatic <em>Plasmodium</em> parasitemia episodes, and 19 bacteria and 3 fungi were associated with resistance.</div></div><div><h3>Conclusion</h3><div>Further studies are needed to confirm these findings, which point the way to strategies aimed at reducing the risk of malaria by modulating gut microbiota components in at-risk populations.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101586"},"PeriodicalIF":2.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mogibacterium bacteraemia in an elderly woman with severe periodontal disease","authors":"Victoria Jordan ,&nbsp;Syeda Naqvi","doi":"10.1016/j.nmni.2025.101585","DOIUrl":"10.1016/j.nmni.2025.101585","url":null,"abstract":"","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101585"},"PeriodicalIF":2.9,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative microbial risk assessment of Legionella pneumophila in a drinking water distribution system: A case study","authors":"Seyed Mohammad Ranjdoost ,&nbsp;Mina Owrang","doi":"10.1016/j.nmni.2025.101584","DOIUrl":"10.1016/j.nmni.2025.101584","url":null,"abstract":"<div><h3>Background</h3><div><em>Legionella pneumophila</em> poses a significant health risk in hospital water systems. This study assessed the risk associated with <em>Legionella</em> contamination in a hospital drinking water system in Sari, Iran, over one year.</div></div><div><h3>Methods</h3><div>Water samples were collected seasonally from various hospital taps, including patient room showers and toilet faucets. Both cold and warm water sources were analyzed. Water quality parameters, including pH, chlorine levels, and temperature, were measured. <em>Legionella</em> spp. were isolated and enumerated using standard microbiological techniques, and species identification was confirmed via 16S rRNA gene sequencing. A Quantitative Microbial Risk Assessment (QMRA) model was employed to estimate the infection risk from shower and faucet use.</div></div><div><h3>Results</h3><div><em>Legionella</em> counts were significantly higher in warm water samples and during the summer season. A positive correlation was observed between <em>Legionella</em> counts and water pH, whereas negative correlations were found with chlorine levels and water temperature. QMRA results indicated that the estimated annual infection risk exceeded the acceptable limits set by the World Health Organization (WHO) and the United States Environmental Protection Agency (USEPA), particularly during summer.</div></div><div><h3>Conclusions</h3><div>The findings suggest that existing water management practices may be inadequate for controlling <em>Legionella</em> growth and transmission. Seasonal variations significantly impact infection risk, emphasizing the need for improved monitoring and control strategies. However, limitations related to sampling methodology, geographic specificity, and dose-response modeling should be considered when interpreting the results.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101584"},"PeriodicalIF":2.9,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reemergence of Oropouche virus infection in Venezuela, 2025","authors":"Alfonso J. Rodriguez-Morales ,&nbsp;Juan-Carlos Navarro ,&nbsp;Alberto Paniz-Mondolfi ,&nbsp;David A. Forero-Peña ,&nbsp;Daniel Romero-Alvarez ,&nbsp;Laura Naranjo-Lara ,&nbsp;Jose A. Suárez","doi":"10.1016/j.nmni.2025.101583","DOIUrl":"10.1016/j.nmni.2025.101583","url":null,"abstract":"","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101583"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143792466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferritin's role in infectious diseases: Exploring pathogenic mechanisms and clinical implications","authors":"Yingying Liao ,&nbsp;Tao Zeng ,&nbsp;Xiaoyan Guo,&nbsp;Xinhua Li","doi":"10.1016/j.nmni.2025.101582","DOIUrl":"10.1016/j.nmni.2025.101582","url":null,"abstract":"<div><div>Ferritin, an iron storage protein, is crucial for maintaining iron metabolism balance throughout the body and serves as a key biomarker for evaluating the body's iron reserves. Reduced ferritin levels typically indicate iron deficiency, whereas elevated ferritin levels indicate an acute inflammatory response in infectious diseases. Recent research has established a significant link between elevated ferritin levels and disease severity and prognosis. The concept of hyperferritinemic syndrome has underscored ferritin's role as a pathogenic mediator. During infections, ferritin not only serves as a biomarker of inflammation but also exerts pro-inflammatory functions, which is a key factor in perpetuating the vicious pathogenic cycle. This review offers a comprehensive exploration of ferritin, covering its structural characteristics, regulatory mechanisms, and how diverse pathogens modulate ferritin. Understanding its pivotal role in infectious diseases is essential for identifying novel therapeutic prospects and enhancing disease management and prevention.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101582"},"PeriodicalIF":2.9,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case of Flavobacterium lindanitolerans meningitis and bacteraemia in an immunocompetent host","authors":"Amelia Benjamin,&nbsp;Robert Gray,&nbsp;Madhuri Vidwans","doi":"10.1016/j.nmni.2025.101581","DOIUrl":"10.1016/j.nmni.2025.101581","url":null,"abstract":"<div><div><em>Flavobacterium lindanitolerans,</em> a gram-negative, non-spore forming predominantly aerobic rod, is an unusual cause of disease in humans. It has previously been described as an opportunistic pathogen in immunocompromised hosts. Here we described a case of meningitis and bacteraemia, in an immunocompetent individual who had undergone recent neurosurgical intervention.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101581"},"PeriodicalIF":2.9,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143737843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case of tinea nigra in children","authors":"Tengteng Xin ,&nbsp;Jiahao Li ,&nbsp;Li Lin,&nbsp;Yijin Luo,&nbsp;Wenying Cai,&nbsp;Junmin Zhang","doi":"10.1016/j.nmni.2025.101577","DOIUrl":"10.1016/j.nmni.2025.101577","url":null,"abstract":"<div><div>A 6-year-old girl presented with brown patches on her right palm that had persisted for 5 years. Based on clinical manifestations, dermoscopy, and fungal microscopy, the child was diagnosed with tinea nigra. Molecular sequencing confirmed the pathogen as <em>Hortaea werneckii</em>. The patient was successfully treated with the topical application of an antifungal ointment.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"65 ","pages":"Article 101577"},"PeriodicalIF":2.9,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}