New Microbes and New Infections最新文献

{"title":"Re-emergence of Nipah Virus in Eastern India: Urgent need for enhanced surveillance","authors":"Chiranjib Chakraborty , Manojit Bhattacharya , C. George Priya Doss , Shyam Sundar Nandi","doi":"10.1016/j.nmni.2026.101718","DOIUrl":"10.1016/j.nmni.2026.101718","url":null,"abstract":"","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101718"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146174247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycobacterium polyniensis sp.nov, a non-tuberculous mycobacterium clinical isolate from Tahiti, French Polynesia","authors":"M.L. Keita ,&nbsp;M. Morsli ,&nbsp;M. Drancourt ,&nbsp;M. Levy ,&nbsp;G. Grine","doi":"10.1016/j.nmni.2025.101691","DOIUrl":"10.1016/j.nmni.2025.101691","url":null,"abstract":"<div><div>In this study, we investigated a previously unidentified respiratory tract isolate, CSUR_Q5927, collected in Tahiti, French Polynesia. Through a comprehensive polyphasic approach combining phenotypic traits with whole genome sequencing, we sought to elucidate the identity and characteristics of this isolate. After cultivating colonies in Middlebrook 7H10 at 37 °C for nine days, we observed acid-fast bacilli under microscopic examination and Ziehl-Neelsen staining. Additionally, electron microscopy revealed the presence of unsporulated bacilli, approximately 1.27 μm ± 0.26 μm in size, displaying morphology consistent with that of a mycobacterium. Matrix-assisted laser desorption/ionization mass spectrometry provided an identification score of 1.42, indicating a low confidence level. However, clustering analysis grouped isolate CSUR_Q5927 within the <em>Mycobacterium terrae</em> complex. Whole genome sequencing unveiled a CG-content of 68.5 % and a coding ratio of 93.6 %, comprising 4337 genes encoding proteins, 55 tRNA genes, and two rRNA. Further comparison with reference strain <em>Mycobacterium terrae</em> NCTC 10856 using DNA-DNA hybridisation values revealed a similarity of 35.8 %, confirming the presence of a new species within the <em>M. terrae</em> complex. In vitro susceptibility testing demonstrated the isolate's susceptibility to eight antimicrobials and resistance to five others. After meticulously conducting appropriate negative controls and analysing all the data, we propose the name <em>Mycobacterium polyniensis</em> for this novel <em>M. terrae</em> species, with isolate CSUR_Q5927 serving as the prototype strain. This discovery expands our understanding of the microbial diversity in the Polynesian region and highlights the importance of combining advanced techniques to accurately characterise and classify previously unknown mycobacteria species.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101691"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surveillance of zoonotic pathogens in cattle and dromedaries sacrificed at the Grand Magal of Touba: a cross-sectional survey","authors":"Ihssane Ouaddane ,&nbsp;Coumba Diouf ,&nbsp;Georges Diatta ,&nbsp;Adama Zan Diarra ,&nbsp;Mbayang Faye ,&nbsp;Cheikh Sokhna ,&nbsp;Philippe Gautret","doi":"10.1016/j.nmni.2026.101714","DOIUrl":"10.1016/j.nmni.2026.101714","url":null,"abstract":"<div><h3>Introduction</h3><div>Mass gatherings increase infectious disease risks through human, environmental, and zoonotic pathways. The Grand Magal of Touba (GMT) involves the annual sacrifice of animals under limited biosafety, yet data on zoonotic pathogens are scarce. This cross-sectional exploratory study aimed to detect major zoonotic agents in livestock during the GMT and to provide baseline evidence to guide One Health surveillance.</div></div><div><h3>Materials and methods</h3><div>From 2022 to 2024, post-mortem samples (blood, nasal and rectal swabs, skin, ticks) were collected from sacrificed animals and analyzed using molecular and culture-based methods.</div></div><div><h3>Results</h3><div>88 animals were included. <em>Borrelia</em> spp. was detected in 28.8 % of bovine blood samples, mostly in 2023 and 8.3 % of dromedaries. Enterohemorrhagic <em>Escherichia coli</em> occurred in 64.3 % of cattle and 33.3 % of dromedaries’ rectal samples. Nasal swabs showed <em>Mycobacterium</em> spp in 54.9 % bovines and 75.0 % dromedaries. Among hard ticks from bovines (predominantly <em>Hyalomma</em> spp.), 10.2 % and 11.5 %) were infected with <em>Borrelia</em> spp. and <em>Rickettsia</em> spp., including <em>R. aeschlimannii</em> and <em>R. sibirica mongolitimonae</em>. and one case of <em>Coxiella burnetii</em> was found. In dromedaries, only <em>Borrelia</em> spp. (43.7 %) was found in ticks. No dermatophytes were isolated.</div></div><div><h3>Conclusion</h3><div>These findings demonstrate active zoonotic circulation and stress the need for continuous surveillance of zoonotic pathogens in animals involved in the GMT. Given the close proximity of humans and livestock during such events, the potential for spillover of zoonotic agents necessitates a <em>One Health</em> approach, integrating veterinary, environmental, and human health surveillance. Future investigations should further characterize pathogen species to better inform risk reduction strategies.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101714"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rare case of breakthrough tick-borne encephalitis in early adolescence after vaccination with four doses of TBE vaccine","authors":"S. Morgardt ,&nbsp;M. Veje ,&nbsp;T. Bergström ,&nbsp;L. Ivarsson ,&nbsp;M. Studahl","doi":"10.1016/j.nmni.2026.101720","DOIUrl":"10.1016/j.nmni.2026.101720","url":null,"abstract":"<div><div>Tick-borne encephalitis (TBE) is an emerging infectious disease with effective vaccines available. However, breakthrough TBE occurs in previously vaccinated patients, a condition that is difficult to diagnose. We report a case of a previously healthy teenager who fell severely ill and was hospitalized for three months despite full TBE vaccination. Initial fever and headache progressed during the first week to encephalitis and, since autoimmune encephalitis was suspected, treatment with immunoglobulins and methylprednisolone was given. This case demonstrates the delayed response of TBEV IgM, typical for breakthrough infection, and the diagnostic value of an early IgG response to the non-structural protein 1 (NS1) in the cerebrospinal fluid. It also discusses the diagnostic challenges, the phenomenon of serological cross reactions with other flaviviruses, and the potential effect of immunomodulatory treatment in TBE. Serial MRI examinations of the brain were performed and confirmed persistent changes years after onset. The reasons for the severe outcome are discussed, but remain unclear.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101720"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146174159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recurrent Nipah virus infections: Implications for preparedness and public health response","authors":"Kevin Smith P. Cabuhat ,&nbsp;Christian Joseph N. Ong ,&nbsp;Jerico B. Ogaya ,&nbsp;Don Eliseo Lucero-Prisno III ,&nbsp;Rich Milton R. Dulay","doi":"10.1016/j.nmni.2026.101719","DOIUrl":"10.1016/j.nmni.2026.101719","url":null,"abstract":"","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101719"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146174248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the anti-quorum sensing potential of synthetic lipstick dyes against Pseudomonas aeruginosa","authors":"Anshula Narayanasamy ,&nbsp;Abinaya Kanagaraja ,&nbsp;Anantha Krishnan Dhanabalan ,&nbsp;Pradeep Sudhakaran ,&nbsp;Munusamy Thirumavalavan ,&nbsp;Palaniyandi Velusamy ,&nbsp;Periasamy Anbu ,&nbsp;Pachaiappan Raman","doi":"10.1016/j.nmni.2026.101708","DOIUrl":"10.1016/j.nmni.2026.101708","url":null,"abstract":"<div><div>Lip care products such as balms, glosses, and lipsticks are widely used to enhance lip health, appearance, and protection. Lipsticks provide aesthetic appeal, social and psychological benefits, that act as a protective barrier against environmental stressors. However, growing concerns have emerged regarding chronic lip infections associated with multidrug-resistant bacteria, particularly <em>Pseudomonas aeruginosa</em>. This is especially concerning in individuals with conditions such as juvenile bullous pemphigoid, hypochromic microcytic anemia, and sickle cell trait. The persistence of <em>P. aeruginosa</em> is largely attributed to its quorum sensing systems, which regulate its virulence. This study explores the anti-QS potential of synthetic dyes found in lipsticks, such as acid red 92, brilliant blue FCF, eosin yellow, titanium dioxide, and carmine, against <em>P. aeruginosa</em>. By the broth dilution method, the minimum inhibitory concentrations (MICs) and sub-MIC levels were determined, and the effects on virulence factors, such as biofilm, pyocyanin, elastase, and rhamnolipid, were assessed by biochemical assays. Further, biofilms formation was visualized using confocal laser scanning microscopy, and gene expression analysis revealed a significant QS inhibition by acid red 92. <em>In-silico</em> analysis by molecular docking studies inferred the potential binding of the dyes with the QS-regulating proteins. Thus, our findings highlight acid red 92 as a promising candidate for therapeutic lip products, offering both cosmetic and anti-QS properties.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101708"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and characterization of bacterial rectal colonization patterns in pediatric patients: A cross-sectional study","authors":"Aynaa Al-Khatib ,&nbsp;Amal Naous ,&nbsp;Elie Salem Sokhn","doi":"10.1016/j.nmni.2026.101707","DOIUrl":"10.1016/j.nmni.2026.101707","url":null,"abstract":"<div><h3>Background</h3><div>Antimicrobial resistance is a major public health concern requiring ongoing surveillance to determine its extent and associated factors. This study evaluates bacterial isolates from pediatric patients in terms of distribution, resistance mechanisms, and demographic/clinical correlations.</div></div><div><h3>Methods</h3><div>In this cross-sectional study, after obtaining parental assent, rectal swabs were aseptically collected from children by pediatric doctors at Healthcare Centers in Beirut. Necessary data were collected through validated questionnaires provided to parents. Bacterial identification was performed using standard culture, isolation, and biochemical techniques. Susceptibility of bacterial strains was studied using antimicrobial susceptibility testing. Finally, Polymerase Chain Reaction (PCR) was used to characterize resistance genes. Associations between resistance phenotypes and sample characteristics were assessed using chi-square or Fisher's exact test, as appropriate.</div></div><div><h3>Results</h3><div>Among 118 rectal isolates, <em>Escherichia coli</em> predominated (75.4 %), followed by other Enterobacterales and <em>Staphylococci</em>. Overall, 31.36 % of isolates harbored a major resistance mechanism (ESBL, AmpC, CRE, or MRSA), with ESBL-producing <em>E. coli</em> being the most frequent (22 %) and <em>bla</em>CTX-M detected in the majority of ESBL isolates. Previous antacid intake was the only characteristic significantly associated with resistance phenotypes (p = 0.03). AmpC, CRE, and MRSA remained relatively infrequent but represented clinically important resistance mechanisms.</div></div><div><h3>Conclusion</h3><div>Rectal colonization with multidrug-resistant organisms was frequent, driven mainly by ESBL-producing, <em>bla</em>CTX-M–positive <em>E</em>. <em>coli</em>, while AmpC, CRE, and MRSA were less common but remained important resistance mechanisms. The association between resistance phenotypes and prior antacid use highlights the need for larger pediatric surveillance studies and careful evaluation of antacid and antibiotic use in this population.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101707"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146025441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological profile of patients undergoing microbiological testing for tuberculosis at a tertiary hospital in the Jazan region, Saudi Arabia: A retrospective study","authors":"Sarah Salih ,&nbsp;Badria Said ,&nbsp;Zakaria Shubayli ,&nbsp;Osama Mobarki ,&nbsp;Albaraa Hakami ,&nbsp;Moataz Zugail ,&nbsp;Abdulaziz Zaylaee ,&nbsp;Ahmed Majrashi ,&nbsp;Jaber Mohammed Hakami ,&nbsp;Moayad Haddad ,&nbsp;Abdulaziz H. Alhazmi","doi":"10.1016/j.nmni.2026.101722","DOIUrl":"10.1016/j.nmni.2026.101722","url":null,"abstract":"<div><h3>Background</h3><div>Tuberculosis (TB) is a contagious bacterial disease that primarily affects the lungs and other parts of the body. This study investigates the epidemiological profile of patients tested for TB at a tertiary hospital in Saudi Arabia<strong>.</strong></div></div><div><h3>Methods</h3><div>The descriptive retrospective study examined the epidemiological profile of 1093 patients, who underwent TB testing at a tertiary hospital in Jazan from January 2020 to December 2022. Data on demographics, diagnoses, and test results were collected from hospital records.</div></div><div><h3>Results</h3><div>Among the 1093 subjects, 59.4% were males, and 68% were Saudi. The mean age was 40.12 years. The test results showed positive TB results among 13.4 patients per 100 individuals. Non-Saudi nationalities were significantly associated with positive test results. Younger age groups had higher positivity rates, with a mean age of 34.83 ± 15.47 years for positive cases. TB positivity rate varied over the three years, peaking in 2021, with the highest positive results in June, October, and November.</div></div><div><h3>Conclusions</h3><div>The higher TB positivity rate among non-Saudi individuals highlights the need for targeted screening and intervention efforts for migrant populations. Furthermore, the increased positivity in younger age groups suggests that additional focus on early detection in this demographic is essential.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101722"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146174245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole genome analysis of carbapenem resistant Acinetobacter baumannii isolates from healthcare-associated infections in Dakar, Senegal","authors":"Issa Ndiaye ,&nbsp;Ousmane Sow ,&nbsp;Abdoulaye Cissé ,&nbsp;Bissoume Sambe Ba ,&nbsp;Farma Thiam ,&nbsp;Mouhamadou Moustapha Boye ,&nbsp;Baidy Dièye ,&nbsp;Cheikh Fall ,&nbsp;Yakhya Dieye ,&nbsp;Assane Dieng ,&nbsp;Amadou Diop ,&nbsp;Guillaume Constantin de Magny ,&nbsp;Abdoulaye Seck","doi":"10.1016/j.nmni.2026.101713","DOIUrl":"10.1016/j.nmni.2026.101713","url":null,"abstract":"<div><h3>Background</h3><div>Carbapenem resistant <em>Acinetobacter baumannii</em> (CRAB) is considered a top priority pathogen by the World Health Organization and is also considered a leading cause of healthcare-associated infections (HAIs). This study aimed to investigate antimicrobial resistance (AMR) patterns and perform comprehensive whole-genome analysis on CRAB strains isolated from HAIs in Dakar, Senegal.</div></div><div><h3>Methods</h3><div>CRAB isolates were collected from 2018 to 2021 in two major hospitals in Dakar and subjected to antimicrobial susceptibility tests followed by whole genome sequencing and comprehensive bioinformatic analysis to identify resistance genes, mobile genetic elements (MGEs) and perform phylogeny analysis.</div></div><div><h3>Results</h3><div>The isolates demonstrated an alarming level of resistance to multiple classes of antibiotics, categorizing them as extremely drug resistant. Genetically, they present 5 distinct sequence types (ST1, ST52, ST85, ST107, and ST164), and an average of 71.9% accessory genes. They also present 30 to 51 genes associated with resistance to numerous classes of antibiotics. These genes included those responsible for resistance to β-lactams (such as <em>bla</em>_{<em>OXA-23</em>}, <em>bla</em>_{<em>NDM-1</em>}, <em>bla</em>_{<em>OXA-51</em>} and <em>bla</em>_{<em>CTX-M15</em>}), to aminoglycosides (<em>ant(3″)-IIa</em>, <em>aph(3″)-Ib</em>, <em>aph(3′)-Ia</em>), to fluoroquinolones (such as <em>qnrS1</em>, <em>qnrB17</em>, <em>AdeFGH</em> efflux pump), to fosfomycin (<em>fos2A</em>, <em>fosA6</em>, <em>AbaF</em>), and to tetracyclines (<em>tet(A)</em>, <em>tet(B)</em>, <em>tet(39)</em>).</div></div><div><h3>Conclusion</h3><div>This pioneering study offers insight into the molecular mechanisms underlying carbapenem-resistant bacteria associated with HAIs in Senegal. These findings emphasize the critical need to implement surveillance programs particularly for carbapenem-resistant bacteria to gain a comprehensive understanding of their prevalence, propagation, impact on patient health outcomes, and prolonged hospital stays.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101713"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146174158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enterococcus thailandicus: Genomic evidence of a potential new player in enterococcal virulence and antimicrobial resistance","authors":"Juan C. Vázquez-Ucha ,&nbsp;Ana C. Almeida-Santos ,&nbsp;David Velasco ,&nbsp;Salud Rodríguez-Pallares ,&nbsp;Michelle Outeda-García ,&nbsp;Arianna Rodríguez-Coello ,&nbsp;Ana Fernández ,&nbsp;Pablo Aja-Macaya ,&nbsp;Carla Novais ,&nbsp;Alejandro Beceiro ,&nbsp;Luisa Peixe ,&nbsp;Ana R. Freitas ,&nbsp;Germán Bou","doi":"10.1016/j.nmni.2026.101711","DOIUrl":"10.1016/j.nmni.2026.101711","url":null,"abstract":"<div><h3>Background</h3><div><em>Enterococcus thailandicus</em> has been isolated from animals, fermented foods, wastewater, and humans, but its clinical relevance remains unclear. We investigated the pathogenic potential of <em>E. thailandicus</em> by phenotypic and genomic characterization of two human isolates from acute abdominal infections, complemented by a species-wide comparative genomic analysis of all publicly available <em>E. thailandicus</em> genomes.</div></div><div><h3>Methods</h3><div>Two <em>E. thailandicus</em> isolates were recovered from patients with acute cholecystitis and small bowel perforation. Species identification was performed by MALDI-TOF MS and ANI analysis, and antimicrobial susceptibility testing was conducted. Whole-genome sequencing (WGS) was performed, followed by <em>in silico</em> analysis of antimicrobial resistance genes, virulence factors, bacteriocins, and plasmid replicons. All available <em>E. thailandicus</em> genomes were included for phylogenetic comparison.</div></div><div><h3>Results</h3><div>Both clinical isolates were broadly susceptible to antimicrobials. WGS revealed the presence of the <em>ptsD</em> gene (linked to hospital-adapted <em>E. faecium</em>) and the bacteriocin gene <em>enkB</em> in both isolates. One isolate carried the <em>tet(M)</em> gene and a plasmid replicase associated with <em>E. faecium</em> plasmids. Among 46 genomes analyzed, 61 % carried <em>ptsD</em>, all carried <em>enkB</em>, and 48 % harbored acquired AMR genes, including functional <em>optrA</em> (n = 12) and <em>poxtA</em> (n = 5), both conferring linezolid resistance. Phylogenetic analysis showed wide genetic diversity with clustering by host or geography, consistent with ecological versatility.</div></div><div><h3>Conclusions</h3><div>This study reports the first genomic characterization of clinical <em>E. thailandicus</em> isolates, demonstrating its ecological adaptability, opportunistic pathogenic potential, and role as a reservoir of clinically relevant adaptive traits. Expanded genomic surveillance is warranted to clarify its evolutionary trajectory and potential impact on human health.</div></div>","PeriodicalId":38074,"journal":{"name":"New Microbes and New Infections","volume":"70 ","pages":"Article 101711"},"PeriodicalIF":5.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146079509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}