MicroRNA (Shariqah, United Arab Emirates)最新文献

{"title":"Exploring the Role of Non-Coding RNAs in the Gut and Skin Microbiome: Implications for Colorectal Cancer and Healthy Longevity.","authors":"Soumya V S, Chaithra Prasad, Sreejith Parameswara Panicker","doi":"10.2174/0122115366342509250401043719","DOIUrl":"https://doi.org/10.2174/0122115366342509250401043719","url":null,"abstract":"<p><p>In the last forty years, cancer mortality rates have risen by more than 40%, with colo-rectal cancer (CRC) ranking as the third most common kind worldwide, significantly affected by dietary factors. Restricted access to sophisticated medical treatment and insufficient comprehen-sion of colorectal cancer's biology contribute to its elevated occurrence. Researchers have recog-nized dysbiosis of the gut microbiome as a critical contributor to the development of colorectal cancer, as it influences the expression of non-coding RNAs (ncRNAs) and subsequent molecular pathways essential for tumor proliferation. Moreover, interactions between gut and skin microbi-ota can impact systemic health and ncRNA regulation, influencing CRC advancement. This study shows how important the gut-skin microbiome axis is in developing colorectal cancer. It suggests that targeting this axis may lead to new treatments, such as changing the microbiome through probiotics, prebiotics, or fecal microbiota transplantation. Nonetheless, we must address obstacles such as population heterogeneity and intricate microbiome-host interactions to facilitate the tran-sition of these medicines into clinical practice. This study seeks to elucidate the roles of dietary treatments, microbiomes, and ncRNAs in the etiology and prevention of colorectal cancer (CRC).</p>","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Exosomal Non-Coding RNAs In Vivo and In Vitro Studies in Colorectal Cancer.","authors":"Mohamed Y Zaky, Hadeer M Hamdalla","doi":"10.2174/0122115366331268250303031240","DOIUrl":"https://doi.org/10.2174/0122115366331268250303031240","url":null,"abstract":"<p><p>Colorectal Cancer (CRC) is the third most lethal cancer worldwide. Complex intercel-lular communication within the tumor microenvironment influences cancer progression, thera-peutic resistance, with Exosomes (Exos) and Circulating Extracellular Vesicles (EVs) playing a critical role in this communication. Exosomes can impact recipient cells by carrying various bio-molecules, promoting changes that support cancer progression. This review focuses specifically on exosome-derived noncoding RNAs (ncRNAs) in CRC, including microRNAs (miRNAs]), circular RNAs (circRNAs), and long noncoding RNAs (lncRNAs), as significant regulators of cancer biology. The roles of these exosomal ncRNAs in CRC are central to tumor progression, metastasis, and treatment resistance. This review delves into specific molecular mechanisms, such as exosomal lncRNA H19, which enhances CRC chemoresistance by activating the β-catenin pathway, and exo-miR-21, which is implicated in 5-FU chemoresistance. We also high-light emerging evidence on exosomal circRNAs like circ_0006174, linked to doxorubicin re-sistance through miR-1205/CCND2 axis modulation. These exo-ncRNAs have shown promise as biomarkers and potential therapeutic targets, with studies indicating their diagnostic and prog-nostic capabilities in CRC patient cohorts. By examining recent in vivo and in vitro studies, we offer a comprehensive understanding of exosomal ncRNAs' roles in CRC pathogenesis and po-tential applications in clinical trials.</p>","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143664902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA-Mediated Regulation of Cancer Stem Cells: Implications for Radioresistance and Chemoresistance in Cancer Treatment.","authors":"Vasanth Kanth Thasma Loganathbabu, Harin N Ganesh, Ravi Gor, Kandasamy Nagarajan Aruljothi, Satish Ramalingam","doi":"10.2174/0122115366300151250107091224","DOIUrl":"https://doi.org/10.2174/0122115366300151250107091224","url":null,"abstract":"<p><p>MicroRNAs (miRNAs) are a class of noncoding RNAs that regulate gene expression in cancer cells and Cancer Stem Cells (CSCs), and play an indispensable role in the development of resistance to radiotherapy and chemotherapy in cancer treatment. The dysregulation of miRNAs in CSCs plays a vital role in influencing the features of CSCs and their resistance mechanisms in different forms of cancer. These miRNAs are also engaged in regulating important signaling pathways, such as Notch and Wnt, which have an impact on the behavior of CSCs and their response to treatment. The intricate network of miRNAs and their interactions with essential proteins and major pathways highlight their potential as therapeutic targets for controlling cancer stem cell behavior and improving the outcomes of cancer therapy by understanding their effects. Comprehending the complex relationship among the production of microRNA, the environment surrounding the tumor, and the processes by which cells communicate can provide vital knowledge for developing successful treatments to overcome resistance to chemotherapy and radiation in cancer therapy.</p>","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143459978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing miRNAs' Janus-Faced Nature: Transforming Cold Tumours into Immunotherapy Hotspots and Overcoming Chemoresistance.","authors":"Shinjini Sen, Ranu Nayak, Banashree Bondhopadhyay, Sudeep Bose","doi":"10.2174/0122115366340113241216043551","DOIUrl":"https://doi.org/10.2174/0122115366340113241216043551","url":null,"abstract":"<p><p>MicroRNA (miRNA) modulation has emerged as a promising strategy in cancer immunotherapy, particularly in converting \"cold\" tumors with limited immune cell infiltration into \"hot\" tumors responsive to immunotherapy. miRNAs regulate immune cell recruitment and activation within the tumor microenvironment, influencing tumor behavior targeting specific miRNAs in cold tumors aims to enhance the immune response, potentially improving therapeutic efficacy. Despite ongoing research challenges, such as tumor complexity and treatment resistance, miRNA-based therapies offer personalized approaches with potential ethical considerations. Advances in miRNA profiling may enable early cancer detection and tailored treatments, underscoring its role in future oncology. This review sheds light on the role of miRNA in cold and hot tumor microenvironments, how they modulate depending on the tumor niche and the current research challenges in implementing miRNA-based therapies include the complexity of tumors and their heterogeneity, which makes it difficult to identify the most relevant miRNAs to target. Additionally, treatment resistance can develop over time, reducing the effectiveness of miRNA modulation. Despite these challenges, ongoing research and advancements in miRNA profiling hold promise for overcoming these obstacles and improving the outcomes of cancer immunotherapy. To overcome the challenges of identifying relevant miRNAs to target, researchers can employ high-throughput sequencing techniques to comprehensively profile miRNA expression in different tumor subtypes. They can also utilize bioinformatics tools and databases to analyze the vast amount of miRNA-related data and identify potential candidate miRNAs. Furthermore, collaborations between scientists and clinicians can help validate the functional significance of identified miRNAs and their potential as therapeutic targets.</p>","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143068465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Hub Genes and Analysis of their Regulatory miRNAs in Patients with Thymoma Associated Myasthenia Gravis Based on TCGA Database.","authors":"Wei Zhou, Jia Hu, Jun Nie","doi":"10.2174/0122115366299210240823062457","DOIUrl":"10.2174/0122115366299210240823062457","url":null,"abstract":"<p><strong>Background: </strong>Myasthenia gravis is an autoimmune disease, and 30% of patients with thymoma often have myasthenia gravis. Patients with thymoma-associated MG (TAMG) have many different clinical presentations compared to non-MG thymoma (NMG), yet their gene expression differences remain unclear.</p><p><strong>Objective: </strong>In this study, we analyzed the Differentially Expressed Genes (DEGs) and analyzed their regulatory microRNAs (miRNAs) in TAMG, which will further clarify the possible pathogenesis of TAMG.</p><p><strong>Methods: </strong>DEGs were calculated using the RNA-sequencing data of TAMG and NMG downloaded from The Cancer Genome Atlas (TCGA) database. R software was then used to analyze the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of DEGs, while STRING was applied to build the protein-protein interaction (PPI) network and Cytoscape to identify and visualize the hub genes. Immune infiltration significances of hub genes were also explored by using the TIMER database and TCGA database. Upstream microRNAs (miRNAs) of the hub genes were predicted by online software.</p><p><strong>Results: </strong>We comparatively analyzed the gene expression differences between TAMG and NMG groups. A total of 977 DEGs were identified between the two groups (|log fold change (FC)| >2, adjusted P value <0.050), with 555 down-regulated genes and 422 up-regulated genes. Five top hub genes (CTNNB1, EGFR, SOX2, ERBB2, and EGF) were recognized in the PPI network. Analysis based on the TIMER and TCGA databases suggested that 5 hub genes were correlated with multiple immune cell infiltrations and immune checkpoint-related markers, such as PDCD1, CTLA-4, and CD274, in TAMG patients. Lastly, 5 miRNAs were identified to have the potential function of regulating the hub gene expression.</p><p><strong>Conclusion: </strong>Our study identified 5 hub genes (CTNNB1, EGFR, SOX2, ERBB2, and EGF) and their 5 regulatory miRNAs in TAMG, and the hub genes were correlated with multiple immune cell infiltrations and immune checkpoint-related markers. Our findings could help partially clarify the pathophysiology of TAMG, which could be new potential targets for subsequent clinical immunotherapy.</p>","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":" ","pages":"49-58"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12246741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142082090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Robust NSCLC Biomarker- miR-7-5p: Its <i>In-Silico</i> Validation and Potential SPR-Based Probe for Detection.","authors":"Chandrajeet Dhara, Anindita Dhara, Saumyatika Gantayat","doi":"10.2174/0122115366325862241031071038","DOIUrl":"10.2174/0122115366325862241031071038","url":null,"abstract":"<p><p>MicroRNA abundance as a particular biomarker for precisely identifying cancer metastases has emerged in recent years. The expression levels of miRNA are analyzed to get insights into cancer tissue detection and subtypes. Similar to other cancer types, the miRNA shows high levels of target mRNA dysregulation in association with non-small cell lung carcinoma (NSCLC). Among many promising cancer biomarkers for NSCLC, miR-7-5p has shown significant downregulation in the NSCLC tissues and targets proto-oncogenes like PAK2 and NOVA2. The expression levels of different proto-oncogenes targeting the miR-7-5p in NSCLC showed that the EGFR-mutated NSCLC has an experimental validation. The target validation of the miR-7-5p could be analyzed using SPR (Surface plasmon resonance) based sensors at a single nanoparticle level, such as Au nanocube, due to its high specificity and accountability. Despite being an accountable tool for cancer diagnosis, miRNA-based biomarkers sometimes cause poor diagnostic specificity and reproducibility due to their heterogenicity and immunogenicity in cancer detection. To overcome these shortcomings, the biomarkers need to be validated according to recent clinical studies.</p>","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":" ","pages":"112-123"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142928473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Key miRNAs in Endometriosis.","authors":"Francesca Blandino, Saviana Antonella Barbati, Luca Forlani, Giulia Coppola, Noemi Meschino, Ilde Cecchinelli, Antonietta Cosco Mazzuca, Valentina Veltri, Riccardo Giannico, Graziella Calugi","doi":"10.2174/0122115366333556241014115206","DOIUrl":"10.2174/0122115366333556241014115206","url":null,"abstract":"<p><strong>Introduction: </strong>Endometriosis, a prevalent gynecological disorder characterized by the presence of endometrial-like tissue outside the uterus, poses significant challenges in diagnosis and management due to its unclear pathogenesis and lack of specific biomarkers.</p><p><strong>Objective: </strong>This study investigates the potential use of microRNAs (miRNAs) as key markers in endometriosis by studying two cohorts of patients (14 patients diagnosed with endometriosis and 15 patients with gynecological benign lesions, different from endometriosis).</p><p><strong>Methods: </strong>MicroRNA sequencing analysis was tested within data management by a custom pipeline designed by Eurofins Genoma Group.</p><p><strong>Results: </strong>We identified a specific miRNA expression profile associated with endometriosis to feature specific disease molecular clusters to further elucidate the underlying mechanisms driving endometriosis pathogenesis. Data from the present study suggest a specific miRNA scar for endometriosis compared to other gynecological diseases to develop screening tools in early diagnosis and to ameliorate the management of the disease itself.</p><p><strong>Conclusion: </strong>This study lays the foundation for the identification of key miRNAs involved in the disease pathogenesis to unveil the molecular signatures in the complex scenario of endometriosis. Further validation and exploration of these findings are needed to develop tools to improve molecular diagnosis and to create a machine-learning prediction algorithm in the future.</p>","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":" ","pages":"170-175"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142548048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Salivary and Serum micro RNA 146a, 200c and its Target Gene PTEN in Chronic Periodontitis Patients and their Response to Non-Surgical Periodontal Therapy.","authors":"Jammula Surya Prasanna, Kunnel Apoorva","doi":"10.2174/0122115366319964241020165218","DOIUrl":"10.2174/0122115366319964241020165218","url":null,"abstract":"<p><strong>Background: </strong>Periodontitis destroys the tooth's supporting structures and attachment apparatus. Local or systemic factors can cause it. Traditionally, diagnosis is based on clinical parameters that may not consistently reflect an accurate confirmation. Biochemical and genetic analyses can provide deeper insights. MicroRNAs (miRNAs) regulate the immune and inflammatory response to microbial pathogens. Detecting and evaluating miRNAs can be an important diagnostic parameter. This study aimed to assess the expression of miRNA 146a,200c, and its target gene PTEN to non-surgical periodontal therapy in serum and saliva.</p><p><strong>Materials and methods: </strong>This interventional comparative study was conducted on 120 patients of both genders, ages between 35 and 55. Non-surgical periodontal therapy (NSPT) scaling and root planing were performed on all subjects, and their saliva and serum samples were collected before and after 8 weeks of NSPT. Quantitative rt-PCR (reverse transcriptase Polymerase Chain Reaction) analysis was conducted on all samples. The statistical analysis was done using SPSS version 22, and comparisons were made using paired t-tests, independent t-tests, and Pearson's correlation coefficient. The statistical significance level was set at a 'P' value of less than 0.05.</p><p><strong>Results: </strong>It has been observed that there was a significant difference of miRNA in both serum and saliva samples 146a,200c, and the PTEN gene expression, from the beginning to 8 weeks. Significant variation was not observed when comparing the levels between serum and saliva.</p><p><strong>Conclusion: </strong>miRNA 146A, 200c, and PTEN genes are interrelated with periodontitis. We can consider them as future biomarkers of periodontal diseases.</p>","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":" ","pages":"136-146"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12645113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142956391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"micro-RNA 451-a as a Circulating Biomarker for Neuroblastoma.","authors":"Aditya Kumar Gupta, Aijaz Ahmad, Disha Kakker, Jagdish Prasad Meena, Ravi Kumar Majhi, Ambreen Jan, Rachna Seth, Venkatesawaran Iyer, Saumyaranjan Mallick","doi":"10.2174/0122115366361597250108075627","DOIUrl":"10.2174/0122115366361597250108075627","url":null,"abstract":"<p><strong>Introduction: </strong>Micro ribonucleic acids (miRNAs) are small non-coding RNAs that modulate the expression of various genes. They have an important role in cancer pathogenesis. Differential expression of multiple miRNAs have been used as potential diagnostic and prognostic markers.</p><p><strong>Methods: </strong>Various miRNAs have lately been employed as diagnostic and therapeutic targets in different cancers. This prospective study included untreated pediatric neuroblastoma (NB) patients. In the discovery phase, global miRNA profiling was done using next-generation sequencing (NGS) on biopsy tissue samples of NB patients. In this phase, the top expressing miRNAs were identified and chosen for further validation as circulating miRNA in blood samples of a different set of NB patients by real-time polymerase chain reaction (PCR).</p><p><strong>Results: </strong>Based on the read counts on the global miRNA profiling in the discovery phase, we found that the miRNA that consistently had high reads across the majority of the NB samples were miRNA 451-a, 19b-3p, 106b-5p, and 21-5p. Of these, we selected miRNA 451-a and 19-b for the validation phase of the study as they had consistent overexpression. In the validation phase, the expression of the circulating miRNA 451-a in the blood was found to be higher. The average value for the relative fold (RF) expression for miRNA 451-a was 1.52.</p><p><strong>Conclusion: </strong>miRNA 451-a is overexpressed both in the cancer tissue and the blood of NB patients. It can serve as a potential diagnostic marker. Further studies can elucidate its role in the pathogenesis of NB and it could have utility as a therapeutic target.</p>","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":" ","pages":"226-233"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142984992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Potential Regulatory Network of Selected Human Erythrocytic miRNAs with <i>Plasmodium falciparum</i> 3D7 mRNAs: A Computational Analysis.","authors":"Urja Joshi, Harsha Motwani, Dhara Jani, Linz-Buoy George, Hyacinth Highland","doi":"10.2174/0122115366321119250123113447","DOIUrl":"10.2174/0122115366321119250123113447","url":null,"abstract":"<p><strong>Background: </strong>miRNAs are small non-coding conserved RNA molecules (18-24 nts) that act as crucial gene regulators via post-transcriptional/translational modifications through interacting with the respective mRNAs during various pathophysiological conditions. Recent research has suggested that non-coding RNAs, particularly miRNAs, can be passed from one species to another to regulate gene expression. Since miRNA-mediated gene regulation has not yet been found in Plasmodia, it is hypothesized that erythrocytic miRNAs from Plasmodium falciparum (P. falciparum) could potentially migrate from the cytoplasm to the parasitophorous vacuole developed intracellularly by the parasite to regulate its transcriptome.</p><p><strong>Objective: </strong>The objective of this study is to investigate the role of trans-kingdom interactions in host-parasite dynamics and their implications for malaria infection.</p><p><strong>Methods: </strong>Using the trans-kingdom target gene prediction tool, psRNA target server, a total of 15 human erythrocytic miRNAs from 12 distinct families were selected and obtained from miRBase to find potential P. falciparum candidate genes. This study utilized ShinyGO (version 0.80) for gene enrichment analysis with statistical analysis of the selected features. The PPI-network analysis was performed using the Maximal Clique Centrality (MCC) approach, along with the CytoHubba plugin for identifying hub nodes. The PPI network was visualized using Cytoscape version 3.7.</p><p><strong>Results: </strong>A total of 145 target genes of Pf3D7 were predicted, with the following genes repeatedly targeted: conserved Plasmodium proteins, conserved Plasmodium membrane proteins, PfEMP1, rifin, RAD54, E3 ubiquitin-protein ligase, and transcription factors related genes. Outputs of ShinyGO included enriched GO pathways of 62 uniquely identified Pf3D7 genes with detailed descriptions and visualized networks. For overlapping relationships, a hierarchical clustering tree of enriched gene sets was carried out, along with a genome plot for representing the chromosomal locations of these genes. According to their coding-noncoding distribution chart, most of these genes were found to be members of the coding gene family. Additionally, PPI-network analysis reported the top 10 hub nodes: PFE0400w, MAL13P1.380, MAL7P1.167, PFD0900w, PF11_0243, PFE0440w, PFE1120w, MAL13P1.315, PF08_0126, and MAL8P1.23. Three KEGG pathway diagrams of pfa 05144 for Malaria, pfa 03440 for homologous recombination, and pfa 00750 for vitamin B6 metabolism with identified Pf3D7 genes were drawn and highlighted in red.</p><p><strong>Conclusion: </strong>The important target genes of Plasmodium falciparum 3D7 were identified by carrying out a trans-kingdom investigation, thus offering preliminary insights into the potential of erythrocytic miRNAs-mediated trans-kingdom regulation.</p>","PeriodicalId":38067,"journal":{"name":"MicroRNA (Shariqah, United Arab Emirates)","volume":" ","pages":"147-169"},"PeriodicalIF":1.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143415616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}