Human Microbiome Journal最新文献_第5页

Comparison of the prokaryotic and eukaryotic microbial communities in peripheral blood from amyotrophic lateral sclerosis, multiple sclerosis, and control populations 肌萎缩性侧索硬化症、多发性硬化症和对照人群外周血原核和真核微生物群落的比较

Human Microbiome Journal Pub Date : 2019-08-01 DOI: 10.1016/j.humic.2019.100060

Jeremy E. Ellis , Dara S. Missan , Matthew Shabilla , Constantine Moschonas , David Saperstein , Delyn Martinez , Christian V. Becker , Stephen E. Fry

{"title":"Comparison of the prokaryotic and eukaryotic microbial communities in peripheral blood from amyotrophic lateral sclerosis, multiple sclerosis, and control populations","authors":"Jeremy E. Ellis , Dara S. Missan , Matthew Shabilla , Constantine Moschonas , David Saperstein , Delyn Martinez , Christian V. Becker , Stephen E. Fry","doi":"10.1016/j.humic.2019.100060","DOIUrl":"10.1016/j.humic.2019.100060","url":null,"abstract":"<div><p>Neurodegenerative diseases are estimated to afflict hundreds of thousands of Americans with vastly more worldwide. The etiologies of amyotrophic lateral sclerosis (ALS) and multiple sclerosis (MS) have yet to be established. Previous studies have suggested an association of these diseases with viruses, bacteria, and eukaryotic microbes, no new therapies have been forthcoming. High-throughput DNA sequencing has enabled the comprehensive analysis of microbial DNA profiles in diseased populations. To date, no amplicon-based next-generation DNA sequencing prokaryotic and eukaryotic community profiling studies have been completed for these diseases. Analysis of peripheral blood samples from control participants as well as ALS and MS participants was used to characterize the hematologic population of microbial DNA. Categorical and multivariate analysis with control for multiple comparisons and aged matched controls revealed differences in microbial DNA contribution in ALS patients compared to others. Notably, sequences that belonging to <em>Ochrophyta</em> were enriched in ALS patient samples. Mechanisms underlying this association, the role of microbial DNA sequences, and the development or progression of ALS may become a fertile subject of inquiry.</p></div>","PeriodicalId":37790,"journal":{"name":"Human Microbiome Journal","volume":"13 ","pages":"Article 100060"},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.humic.2019.100060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42192592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Digestive tract mycobiota and microbiota and the effects on the immune system 消化道菌群和微生物群及其对免疫系统的影响

Human Microbiome Journal Pub Date : 2019-06-01 DOI: 10.1016/j.humic.2019.100056

Nina Gouba , Yeri Esther Hien , Marie Laure Guissou , Maxime Descartes Mbogning Fonkou , Yves Traoré , Zekiba Tarnagda

引用次数: 10

Whole genome metagenomic analysis of the gut microbiome of differently fed infants identifies differences in microbial composition and functional genes, including an absent CRISPR/Cas9 gene in the formula-fed cohort 对不同喂养方式婴儿肠道微生物组的全基因组宏基因组分析发现了微生物组成和功能基因的差异，包括配方奶喂养队列中缺失的CRISPR/Cas9基因

Human Microbiome Journal Pub Date : 2019-06-01 DOI: 10.1016/j.humic.2019.100057

Matthew D. Di Guglielmo , Karl Franke , Courtney Cox , Erin L. Crowgey

{"title":"Whole genome metagenomic analysis of the gut microbiome of differently fed infants identifies differences in microbial composition and functional genes, including an absent CRISPR/Cas9 gene in the formula-fed cohort","authors":"Matthew D. Di Guglielmo , Karl Franke , Courtney Cox , Erin L. Crowgey","doi":"10.1016/j.humic.2019.100057","DOIUrl":"10.1016/j.humic.2019.100057","url":null,"abstract":"<div><h3>Background</h3><p>Advancements in sequencing capabilities have enhanced the study of the human microbiome. There are limited studies focused on the gastro-intestinal (gut) microbiome of infants, particularly the impact of diet between breast-fed (BF) versus formula-fed (FF). It is unclear what effect, if any, early feeding has on short-term or long-term composition and function of the gut microbiome.</p></div><div><h3>Results</h3><p>Using a shotgun metagenomics approach, differences in the gut microbiome between BF (n = 10) and FF (n = 5) infants were detected. A Jaccard distance principle coordinate analysis was able to cluster BF versus FF infants based on the presence or absence of species identified in their gut microbiome. Thirty-two genera were identified as statistically different in the gut microbiome sequenced between BF and FF infants. Furthermore, the computational workflow identified 371 bacterial genes that were statistically different between the BF and FF cohorts in abundance. Only seven genes were lower in abundance (or absent) in the FF cohort compared to the BF cohort, including CRISPR/Cas9; whereas, the remaining candidates, including autotransporter adhesins, were higher in abundance in the FF cohort compared to BF cohort.</p></div><div><h3>Conclusions</h3><p>These studies demonstrated that FF infants have, at an early age, a significantly different gut microbiome with potential implications for function of the fecal microbiota. Interactions between the fecal microbiota and host hinted at here have been linked to numerous diseases. Determining whether these non-abundant or more abundant genes have biological consequence related to infant feeding may aid in understanding the adult gut microbiome, and the pathogenesis of obesity.</p></div>","PeriodicalId":37790,"journal":{"name":"Human Microbiome Journal","volume":"12 ","pages":"Article 100057"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.humic.2019.100057","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39197388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Analysis of blood and fecal microbiome profile in patients with celiac disease 乳糜泻患者血液和粪便微生物组分析

Human Microbiome Journal Pub Date : 2019-03-01 DOI: 10.1016/j.humic.2018.12.001

Gloria Serena , Camron Davies , Murat Cetinbas , Ruslan I. Sadreyev , Alessio Fasano

{"title":"Analysis of blood and fecal microbiome profile in patients with celiac disease","authors":"Gloria Serena , Camron Davies , Murat Cetinbas , Ruslan I. Sadreyev , Alessio Fasano","doi":"10.1016/j.humic.2018.12.001","DOIUrl":"10.1016/j.humic.2018.12.001","url":null,"abstract":"<div><p>Celiac disease is a multifactorial autoimmune enteropathy triggered by ingestion of gluten in genetically predisposed individuals. The increase of incidence in celiac disease suggests that additional environmental factors other than gluten may contribute to its onset and development. While intestinal dysbiosis has already been associated with celiac disease, the role that the blood microbiome plays in the loss of tolerance to gluten is unknown. In this study we aimed at evaluating weather celiac patients are characterized by alterations in the blood microbiome and how these changes may relate to the intestinal microbiome composition and, ultimately, to the loss of tolerance to gluten. Our data highlight alterations in the blood microbiome composition and taxonomic diversity in celiac patients as compared to healthy subjects. Although preliminary, these findings suggest that changes in the blood microbiome may contribute to the pathogenesis of celiac disease and open the possibility of new therapeutic and diagnostic tools for celiac patients.</p></div>","PeriodicalId":37790,"journal":{"name":"Human Microbiome Journal","volume":"11 ","pages":"Article 100049"},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.humic.2018.12.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41959819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Virus and microbiota relationships in humans and other mammals: An evolutionary view 人类和其他哺乳动物中病毒和微生物群的关系:一种进化观点

Human Microbiome Journal Pub Date : 2019-03-01 DOI: 10.1016/j.humic.2018.11.001

Maurício Teixeira Lima, Ana Cláudia dos Santos Pereira Andrade, Graziele Pereira Oliveira, Jacques Robert Nicoli, Flaviano dos Santos Martins, Erna Geessien Kroon, Jônatas Santos Abrahão

引用次数: 11

Current and future targets for faecal microbiota transplantation 粪便微生物群移植的当前和未来目标

Human Microbiome Journal Pub Date : 2019-03-01 DOI: 10.1016/j.humic.2018.08.004

James Roger Mcilroy , Jonathan P. Segal , Benjamin H. Mullish , Mohammed Nabil Quraishi , Antonio Gasbarrini , Giovanni Cammarota , Gianluca Ianiro

{"title":"Current and future targets for faecal microbiota transplantation","authors":"James Roger Mcilroy , Jonathan P. Segal , Benjamin H. Mullish , Mohammed Nabil Quraishi , Antonio Gasbarrini , Giovanni Cammarota , Gianluca Ianiro","doi":"10.1016/j.humic.2018.08.004","DOIUrl":"10.1016/j.humic.2018.08.004","url":null,"abstract":"<div><p>The human gastrointestinal tract is home to the most diverse microbial ecosystem in the human body and is made up of bacteria, viruses and eukarya. Collectively known as the gut microbiota, our knowledge of these microbial communities has historically been restricted by the relative limitations of culturing techniques. However, the recent development and utilisation of next-generation sequencing techniques has enhanced our understanding of its structure, diversity and function.</p><p>There is emerging evidence that the gut microbiota plays a pivotal role in both health and disease. Perturbations to the structure and function of the gut microbiota are known to be associated with certain disease states. Therefore, manipulating the gut microbiota in an attempt to restore structure and function represents a promising therapeutic strategy. Recently, there has been a surge in clinical and scientific interest in manipulating the gut microbiota using a method called faecal microbiota transplantation. This increase in interest has gathered after it was shown in randomised controlled trials to be highly effective in treating recurrent <em>Clostridium difficile</em> infection.</p><p>Despite success in treating recurrent <em>Clostridium difficile,</em> there remain many unknowns about how best to optimise its preparation, regulation, mode of delivery and safety. This review aims to summarise the literature surrounding the current knowledge regarding faecal microbiota transplantation and explore potential future research avenues that aim to enhance the safety, efficacy and utilisation of faecal microbiota transplantation.</p></div>","PeriodicalId":37790,"journal":{"name":"Human Microbiome Journal","volume":"11 ","pages":"Article 100045"},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.humic.2018.08.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49327775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Association of gut dysbiosis with intestinal metabolites in response to antibiotic treatment 肠道生态失调与肠道代谢物对抗生素治疗反应的关联

Human Microbiome Journal Pub Date : 2019-03-01 DOI: 10.1016/j.humic.2018.11.004

Tariq Jamal Khan , Mohammed Nihal Hasan , Esam I. Azhar , Muhammad Yasir

{"title":"Association of gut dysbiosis with intestinal metabolites in response to antibiotic treatment","authors":"Tariq Jamal Khan , Mohammed Nihal Hasan , Esam I. Azhar , Muhammad Yasir","doi":"10.1016/j.humic.2018.11.004","DOIUrl":"10.1016/j.humic.2018.11.004","url":null,"abstract":"<div><p>Gut microbiota (GM) is associated with metabolism, provides energy-harvesting efficiency and protection against opportunistic pathogens through competitive exclusion to the host. Previous studies highlighted the temporary as well as permanent alteration to GM resulting from different antibiotics treatment. The diverse class of antibiotics may damage the metabolic homeostasis and can alter the level of intestinal metabolites [including amino acids, bile acids (BAs), glucose, short chain fatty acids (SCFAs)] through alteration in abundance of metabolically active bacteria. The antibiotics administration causes the disturbed profile of related microbial metabolites, especially that of BAs, primary and secondary BAs (conjugated or unconjugated BAs). The antibiotics intake causes the reduced bacterial diversity that makes the individuals susceptible towards diseases. To a large extent, we tried to clarify the adverse effects of classes of antibiotics on the GM composition, and the consequent impacts of dysbiosis on the BAs feedback loop between liver and gut, which involves the farnesoid-X-receptor-fibroblast growth factor (FXR-FGF) pathway. The current review discusses the antibiotics-GM-BAs nexus during <em>Clostridium difficile</em> infection (CDI) and the recommended therapy includes faecal microbial transplant (FMT) in countering the exposure of harmful antibiotic and bacteriotherapy as an alternative therapeutic intervention in treating the recurrent CDI.</p></div>","PeriodicalId":37790,"journal":{"name":"Human Microbiome Journal","volume":"11 ","pages":"Article 100054"},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.humic.2018.11.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45166471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Exhaustive repertoire of human vaginal microbiota 人类阴道微生物群的详尽曲目

Human Microbiome Journal Pub Date : 2019-03-01 DOI: 10.1016/j.humic.2018.11.002

Khoudia Diop , Jean-Charles Dufour , Anthony Levasseur , Florence Fenollar

{"title":"Exhaustive repertoire of human vaginal microbiota","authors":"Khoudia Diop , Jean-Charles Dufour , Anthony Levasseur , Florence Fenollar","doi":"10.1016/j.humic.2018.11.002","DOIUrl":"10.1016/j.humic.2018.11.002","url":null,"abstract":"<div><p>Bacteria that colonize the vaginal microbiota of women play an important role in health and homeostasis. Disruption of the proportion of bacteria predisposes to dysbiosis like bacterial vaginosis or severe gynecological conditions such as preterm birth, pelvic inflammatory disease and also sexually transmitted diseases. Knowledge about normal and abnormal vaginal microbiota has become a little clearer in recent years. Culture techniques have made it possible to isolate and describe many bacterial species, whereas molecular methods have highlighted the limits of culture by showing that the vagina was a complex ecosystem containing a wide range of non-cultured or difficult-to-identify bacteria. Based on an exhaustive review of the scientific literature, we built the repertoire of all the bacteria found using culture-based and/or independent methods on the human vagina. So, whether they are valid or not, we inventoried 581 bacteria identified in the human vagina distributed into 10 taxa, mainly in the phyla of <em>Actinobacteria</em>, <em>Bacteroidetes</em>, <em>Firmicutes</em>, and <em>Proteobacteria</em> with 206 distinct genera classified in 96 different families. This repertoire is essential for microbiologists and clinicians and represents the starting point for a Vaginal Microbiome Project such a project aimed to map the human vaginal microbiota, to better understand the dysbioses or infections caused by its imbalance in order to offer more appropriate treatments.</p></div>","PeriodicalId":37790,"journal":{"name":"Human Microbiome Journal","volume":"11 ","pages":"Article 100051"},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.humic.2018.11.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43249064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49

Increasing and eliminating the (Fecal coliforms, thermotolerant coliform and fecal streptococcus) bacteria by resin of the poly (para carboxy acid phenol-d-Glucose) to clean up waste water 利用聚对羧酸苯酚-葡萄糖树脂净化废水，增加和消除粪便大肠菌群、耐热大肠菌群和粪便链球菌

Human Microbiome Journal Pub Date : 2019-03-01 DOI: 10.1016/j.humic.2018.11.003

Safia Miloudi , Messaoud Chaib , Moulkheir Ayat , Abdelkader Rahmouni

{"title":"Increasing and eliminating the (Fecal coliforms, thermotolerant coliform and fecal streptococcus) bacteria by resin of the poly (para carboxy acid phenol-d-Glucose) to clean up waste water","authors":"Safia Miloudi , Messaoud Chaib , Moulkheir Ayat , Abdelkader Rahmouni","doi":"10.1016/j.humic.2018.11.003","DOIUrl":"10.1016/j.humic.2018.11.003","url":null,"abstract":"<div><p>In this research paper we represent a novel synthetic antibacterial phenolic polymer (pAP-DG) containing carboxylic acid group in para position. The study of poly (p-carboxylic acid phenol <span>d</span>-Glucose) as an antibacterial resin was focused on the development of antimicrobial polymers to clean up the water of pathogenic bacteria. The polycondensation reaction was realized between p-carboxy acid phenol (pAP) and <span>d</span>-Glucose (DG) as monomers; using sulfuric acid as homogeneous catalyst.</p><p>The obtained polymer was used via <em>fecal coliform</em> (TC), <em>thermotolerant coliforms</em> (CTT) and <em>fecal streptococci</em> (ST) bacteria to evaluate the water quality. The antibacterial activity of the synthesized resin was carried out by the contact method so the capacity of this resin was confirmed. In general, the polymer has a good antimicrobial activity against the microorganisms tested; the resulting polymer was characterized by various spectroscopy methods such as: <sup>1</sup>H NMR, <sup>13</sup>C NMR, DSC and FTIR and a mechanism has been proposed for this polycondensation reaction.</p></div>","PeriodicalId":37790,"journal":{"name":"Human Microbiome Journal","volume":"11 ","pages":"Article 100053"},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.humic.2018.11.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48143019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel targets to develop new antibacterial agents and novel alternatives to antibacterial agents 开发新的抗菌剂和新的抗菌剂替代品的新靶点

Human Microbiome Journal Pub Date : 2019-03-01 DOI: 10.1016/j.humic.2019.01.001

Tafere Mulaw Belete

{"title":"Novel targets to develop new antibacterial agents and novel alternatives to antibacterial agents","authors":"Tafere Mulaw Belete","doi":"10.1016/j.humic.2019.01.001","DOIUrl":"10.1016/j.humic.2019.01.001","url":null,"abstract":"<div><p>Antibacterial agents have saved many lives and helped the growth of modern medicine over the past half century. The emergence of drug resistance, jeopardizing the effectiveness of these life-saving treatments. This clearly highlights the urgent need for new and improved antibacterial drugs with a novel target and new molecular structure agent to obviate cross-resistance. This paper reviewed the possible new ways to discover novel antibacterial agents. The most widely studied new bacterial targets for novel drug development are quorum sensor biosynthesis, bacterial virulence factor, bacteria cell division machinery, Bacterial cell wall synthesis, PDF inhibitor, isoprenoid biosynthesis, shikimate synthesis pathway, biofilm synthesis and fatty acid biosynthesis. These new discovery routes have given rise to agents that are in preclinical trials. This review also discusses the alternatives approaches that act <em>bacteria</em> or any approaches that target the host. The most advanced approaches that are on clinical development are phages and other approaches that are on preclinical development are antimicrobial peptides. These alternatives ways may use as adjunctive therapies, which suggest that conventional antibacterial agents are still essential.</p></div>","PeriodicalId":37790,"journal":{"name":"Human Microbiome Journal","volume":"11 ","pages":"Article 100052"},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.humic.2019.01.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47988710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 66