OpenNano最新文献

{"title":"Carotenoid containing cationic nanoparticles for effective therapy for suppressing oxidative stress: An intranasal approach","authors":"Namdev Dhas ,&nbsp;Atul Garkal ,&nbsp;Ritu Kudarha ,&nbsp;Srinivas Hebbar ,&nbsp;Srinivas Mutalik ,&nbsp;Tejal Mehta","doi":"10.1016/j.onano.2023.100172","DOIUrl":"10.1016/j.onano.2023.100172","url":null,"abstract":"<div><p>Alzheimer's disease is the most common type of dementia. Oxidative stress is involved in the progression of aging and Alzheimer's disease. It is known that lutein is a carotenoid having antioxidant properties. The present research explores Lutein loaded chitosan nanoparticles for Suppressing Oxidative Stress in the treatment of Alzheimer's. The developed nanoparticles are administered through the nose to target brain via nose-to-brain pathway. For optimization of formulation a systematic QbD approach was used. The developed nanoparticles further characterized for Physicochemical parameters, Morphology, In-vitro drug releases, Ex-vivo diffusion, In-vitro Cell viability, cellular uptake, In-vitro BBB Permeation, antioxidant properties, In-vivo biodistribution, and stability study. The developed nanoparticles' surface morphology suggested homogeneously dispersed spherical nanoparticles having &lt; 200 nm size. The drug release study demonstrate the controlled release of lutein for more than 96 h while less than 50% lutein was released after 24 h in an <em>ex-vivo</em> diffusion study. The cell cytotoxicity assay confirms the nontoxicity of <span>l</span>-CNPs. The cellular uptake study shows enhanced internalization of <span>l</span>-CNPs through the caveolae-mediated endocytosis pathway. The ROS generation confirmed the absence of any significant ROS generation nanoparticles. The antioxidant assay shows significant ROS scavenging activity of <span>l</span>-CNPs. <em>In-vitro</em> BBB permeation demonstrates the efficient passage of <span>l</span>-CNPs through BBB compared to pure lutein. This was further supported by bio-distribution demonstrating the deposition of nanoparticles in the brain through nasal administration. The acquired outcomes prove the possible activities of lutein-loaded <span>l</span>-CNPs for reducing oxidative stress in the brain for Alzheimer's treatment.</p></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"13 ","pages":"Article 100172"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42953694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dendrimer-based nanocomposites for the production of RNA delivery systems","authors":"Chiou Chyi Lim,&nbsp;Le Yi Chia,&nbsp;Palanirajan Vijayaraj Kumar","doi":"10.1016/j.onano.2023.100173","DOIUrl":"10.1016/j.onano.2023.100173","url":null,"abstract":"<div><p>A novel approach to treating cancer has been revealed to be effective cancer vaccination. It has also been demonstrated that nucleic acid therapies such as mRNA and siRNA are very efficient in the treatment of cancer. However, the instability of mRNA makes a delivery system necessary to reach the target sites. Dendrimers have emerged as being of important interest in healthcare due to their ideal characteristic of having a very strong drug delivery capacity to become carriers. The dendrimer's center can be loaded with specific pharmaceutical active ingredients, or they can be bonded to the surface. RNA delivery systems that transport siRNA, mRNA, and other forms of RNA can be made using dendrimers. This review article focuses on several dendrimer-based RNA delivery systems, such as dendrimers modified with PEG, mannosylated dendrimers, dendrisomes, amphiphilic dendrimer vectors, magnetic nanoparticles modified with dendrimers, peptide dendrimers, and gold nanoparticles coated with dendrimers.</p></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"13 ","pages":"Article 100173"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45243847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal doped nanocomposites for detection of pesticides and phenolic compounds by colorimetry: Trends and challenges","authors":"Sammi Boher ,&nbsp;Rooh Ullah ,&nbsp;Mustafa Tuzen ,&nbsp;Tawfik A. Saleh","doi":"10.1016/j.onano.2023.100168","DOIUrl":"10.1016/j.onano.2023.100168","url":null,"abstract":"<div><p>Colorimetric methods are classical techniques that have been broadly subjected to and applied for the detection of many analytes. The method is low cost, simple, practically active, and suitable to determine the sample due to color changes which are accessed visually even at low concentrations of target analysis. Hence, different methods are applied for detecting and determining pesticides (insecticides) and phenolic compounds (bisphenol A). Moreover, researchers detected OCPs (Organochloride pesticides) in breast milk and adipose tissues, which may exhibit estrogenic, and antiestrogenic activities mostly associated with breast cancer. The common thing between two the analytes is nature because they both show endocrine-disrupting properties. Bisphenol A (phenolic compound) is commonly used in the high-volume production of monomers and plastics. Bisphenol A is used to manufacture polycarbonate plastics, used in food cans coating, baby formula bottles, milk containers, paints, etc. Pesticides and phenolic compounds identification methods can be sluggish and need extremely skilled workforces to function cultured instruments that are mostly too expensive, delicate, or immense to a position outside of a devoted laboratory facility. This review deals with the discriminatory and low-cost method for the colorimetric detection of pesticides and phenolic compounds (bisphenol A) with different bio-synthesized metals and metal-doped nanocomposites. Based on existing publications, using colorimetry with nanocomposites provides low detection limits and good reproducibility for the detection of pesticides and phenolic compounds in various samples such as food samples. These results serve as a guide for controlling pesticides and phenolic chemicals in food processing, lowering the dangers involved.</p></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"13 ","pages":"Article 100168"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44630649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial activity of nanoformulations of carvacrol and thymol: New trend and applications","authors":"Aida Hajibonabi ,&nbsp;Mina Yekani ,&nbsp;Simin Sharifi ,&nbsp;Javid Sadri Nahad ,&nbsp;Solmaz Maleki Dizaj ,&nbsp;Mohammad Yousef Memar","doi":"10.1016/j.onano.2023.100170","DOIUrl":"10.1016/j.onano.2023.100170","url":null,"abstract":"<div><p>Thymol and carvacrol are aromatic compounds derived from plants that exhibit considerable broad-spectrum antimicrobial effects. They have also shown extensive biological effects, including antispasmodic, anti-inflammatory, and anti-carcinogenic. Carvacrol and thymol also have pleasant smells, tastes, and potent antioxidant effects. Therefore, biological effects, along with their favorable toxicity, make thymol and carvacrol an option as an additive to inhibit microbial spoilage of foods and potent antimicrobial agents against antibiotic-resistant bacteria. However, volatility, low stability, and high hydrophobicity are some of the limitations of carvacrol and thymol, which limit their application. To increase the efficacy of thymol and carvacrol, especially antimicrobial properties, using a drug delivery system might be a practical option. Encapsulation of the essential oils into appropriated nanocarriers may decrease their potential limitations. Carvacrol and thymol-encapsulated nanomaterials have been shown to have more solubility and increased antibacterial effects. Here, we provide a brief review of the antimicrobial effects of carvacrol and thymol nanoformulation to give a prospect on their applications for future studies as natural antimicrobial agents and food additives.</p></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"13 ","pages":"Article 100170"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49177728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategies for lipid-based nanocomposites with potential activity against Mycobacterium tuberculosis: Microbial resistance challenge and drug delivery trends","authors":"Arthur Cavalcante Hatae ,&nbsp;Cesar Augusto Roque-Borda ,&nbsp;Fernando Rogério Pavan","doi":"10.1016/j.onano.2023.100171","DOIUrl":"10.1016/j.onano.2023.100171","url":null,"abstract":"<div><p><em>Mycobacterium tuberculosis</em> (MTB), the causative agent of tuberculosis, stands as an immensely devastating and persistently relevant pathogen, claiming the lives of millions each year. This infectious bacterium remains a formidable global health challenge, necessitating urgent attention and comprehensive strategies to combat its profound impact on public health. MTB is a finicky bacterium that manages to sneak into macrophages and fibroblasts to avoid being eliminated. Current first-line treatments allow the control of the spread of an active MTB, but are not capable of effectively controlling when MTB is in its latent phase and struggle against MTB resistant strains. Lipid-based nanoparticles have gained significant attention in the field of tuberculosis nanotechnology treatments, owing to their compelling logical underpinnings, remarkable merits, and acknowledged demerits. These nanoparticles offer a rational approach by harnessing the unique properties of lipids, such as biocompatibility and stability, to encapsulate and protect anti-tuberculosis drugs. Their inherent ability to actively target infected macrophages holds immense promise for precise drug delivery to the infection site, enhancing therapeutic efficacy. However, it is crucial to consider potential limitations, such as the restricted payload capacity due to their small size and challenges in achieving sustained drug release. Despite these challenges, lipid-based nanotechnology represents an exciting frontier for combating drug resistance and advancing tuberculosis treatment strategies, warranting further exploration and development in this field. In addition, we emphasize the characteristics of lipid-based nanoparticles with the ability to improve the administration, stability, and dosage of these molecules. New modified systems are expected to be successful in the coming years as nanotechnology has improved various treatments in other diseases.</p></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"13 ","pages":"Article 100171"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48482738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advancements in the targeted delivery of Gemcitabine: Harnessing nanomedicine for enhanced cancer therapy","authors":"Mehrab Pourmadadi ,&nbsp;Ali Aslani ,&nbsp;Dilawar Hassan ,&nbsp;Ayesha Sani ,&nbsp;Abbas Rahdar ,&nbsp;Dora I． Medina ,&nbsp;Majid Abdouss ,&nbsp;Luiz Fernando Romanholo Ferreira","doi":"10.1016/j.onano.2023.100177","DOIUrl":"10.1016/j.onano.2023.100177","url":null,"abstract":"<div><p>Gemcitabine (GEM) is a chemotherapeutic drug widely used for treating pancreatic cancer and other cancers. Despite its efficacy, GEM is associated with adverse side effects and tumor resistance, hampering its therapeutic outcomes. To address these challenges, innovative strategies have emerged to enhance GEM delivery. This comprehensive review explores various distribution systems, including polymer-based platforms, liposomes, and inorganic nanoparticles, highlighting their unique characteristics for improving GEM efficacy in cancer treatment. Additionally, we discuss the promising approach of codelivery with genes and present several methods for augmenting GEM's chemotherapeutic properties. Our findings shed light on novel insights and provide recommendations for overcoming the limitations associated with GEM, guiding researchers and clinicians toward more effective cancer therapies.</p></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"13 ","pages":"Article 100177"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48887735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organ-on-a-Chip Microfluidic Systems for Tracking Exosomal Dynamic Communication","authors":"Abhimanyu Thakur","doi":"10.1016/j.onano.2023.100179","DOIUrl":"10.1016/j.onano.2023.100179","url":null,"abstract":"<div><p>Organ-on-a-chip microfluid systems (OCMS) are miniaturized three-dimension models of human tissue and organ, designed to recapitulate the crucial physiological and biological parameters of their corresponding <em>in vivo</em> parts. They have emerged as a powerful multifunctional tool for various applications such as personalized medicine, drug screening, due to its ability to show biomimetic composition, designs, and functions. Recently, OCMS have been employed to model and decode inter-organ communication via exosomes. Exosomes are biological nanovesicles with approximately 30-200 nm diameter, released from most of the cell types and participate in various cellular functions via intracellular communication and by carrying different cargoes including protein, and nucleic acids. Under pathological conditions such as cancer, the release of exosomes enhances tremendously, which are either fused or internalized by the recipient cells to elicit specific biological responses. The research pertaining to the exosomal communication has employed different methods for characterizing their release by the donor cells and uptake by the recipient cells, such as nano tracking analyzer, protein quantification, transmission electron microscopy (TEM), scanning EM (SEM), and immunogold-EM, exosome labeling kits, microbead-based flow cytometry. However, the research associated with the regulation of exosomal release and uptake has been impeded by the dearth of advanced techniques for capturing dynamics of exosomes. Here in, we discuss the advances in biosensing for tracking exosomal dynamic communication in OCMS, which will open new avenues of exosomal research using microfluidic engineering for modeling intracellular communication in OCMS.</p></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"13 ","pages":"Article 100179"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47223839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and Characterization of Alginate Nanocarriers as Mucoadhesive Intranasal Delivery Systems for Ameliorating Antibacterial Effect of Rutin Against Pasteurella Multocida Infection in Mice","authors":"Amany M. Mohamed ,&nbsp;Nagwa I. Toaleb ,&nbsp;Ahmad Mohammad Allam ,&nbsp;Sahar Hussein Abdalla Hekal ,&nbsp;Sherein S. Abdelgayed ,&nbsp;Abeer S. Hassan","doi":"10.1016/j.onano.2023.100176","DOIUrl":"10.1016/j.onano.2023.100176","url":null,"abstract":"<div><p>Rutin is a natural product has various biological activities. Pasteurellosis is crucial bacterial infection of respiratory system caused by <em>Pasteurella multocida</em>. This study aimed to investigate the improved antibacterial effect of Rutin nanocarriers as mucoadhesive intranasal delivery against <em>Pasteurella multocida</em>. Different formulations of Rutin niosomes and nanostructure lipid carriers (NLCs) were formulated and well characterized. The in vivo antibacterial performance of the developed formulations against <em>Pasteurella multocida</em> in infected mice was conducted. Further, cytokines levels of Interferon Gamma (INF-γ) and Interlukin-12 (IL -12) in mice sera were assessed. The results revealed that developed Rutin nanocarriers were in nanosized range and exhibited high drug encapsulation. However, Rutin NLCs showed smaller particle size (240.34 ± 5.5 nm), higher encapsulation% (97.34 ± 0.15%), and higher drug release of 94.5% within 12 h comparing with Rutin niosomes. Further, Rutin NLCs presented the highest antibacterial effect against <em>P. multocida</em> infection compared with other treated groups. The bacterial count in lungs and livers was reduced in treated groups compared to the infected non treated one. Our results indicate that mucoadhesive Rutin nanocarriers introduce a new promising antibacterial agent for intranasal delivery against <em>P. multocida</em> and open vision for veterinary applications to utilize advanced nanocarriers in the management of several infections.</p></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"13 ","pages":"Article 100176"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43937062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of CeO2/spherical silica and halloysite nanotubes engineered for targeted drug delivery system to treat breast cancer cells","authors":"Sarah Almofty ,&nbsp;Vijaya Ravinayagam ,&nbsp;Norah Alghamdi ,&nbsp;Wejdan Alghamdi ,&nbsp;Zainab Albazroun ,&nbsp;Layan Almulla ,&nbsp;Sultan Akhtar ,&nbsp;Ali Awad Almofleh ,&nbsp;Gazali Tanimu ,&nbsp;H. Dafalla ,&nbsp;B. Rabindran Jermy","doi":"10.1016/j.onano.2023.100169","DOIUrl":"10.1016/j.onano.2023.100169","url":null,"abstract":"<div><p>Cerium oxide nanoparticles (CeO₂ NPs) and flavonoid curcumin that has been widely studied for treating diseases involving high reactive oxygen species (ROS). In nanotherapeutics, the particle size, shape, metal oxide dispersity and surface properties of nanocarriers are vital for drug delivery and therapeutic efficiency. Here, cisplatin release behavior on cerium impregnated two different shaped nanocarriers, CeO₂/monodispersed spherical silica (Sil) and CeO₂/halloysite (Hal) nanotube was studied for potential anti-cancer therapies. For comparison, CeO₂ impregnated mesoporous silica MCM-41, SBA-16, Hydroxyapatite and clay were prepared. Subsequently, the nanocomposites were coated with curcumin (25% wt/wt), and cisplatin (Cp) functionalization (5% wt/wt). 5wt%CeO₂/Hal/Cp and 5wt%CeO₂/Sil/Cp samples were pegylated using lyophilization technique. Physico-chemical analyses revealed the nanosized distribution of CeO₂ and functionalization of cisplatin and curcumin. Cp release was studied using automated Franz cell and dialysis membrane techniques. The different structured nanocarriers delivering mechanism was studied by determining the drug kinetic release using four different kinetic models (first order, second order, Higuchi and Korsmeyer-Peppas). <em>In vitro</em> cytotoxicity assay of nano formulations along with free cisplatin and curcumin (Cur) were tested against the breast cancer cell line (MCF-7) for multiple timepoints by MTT assay. The results reveled the efficacy of 5wt%CeO₂/Sil/Cp/Cur nanoparticles in delivering cisplatin. On the other hand, 5wt%CeO₂/Hal/Cur nanoparticles enhanced the uptake of curcumin in comparison to free curcumin. Overall, pegylated CeO₂/Silica nano formulation demonstrated an effective carrier to cisplatin for potential treatment of breast cancer.</p></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"13 ","pages":"Article 100169"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45335433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modelling tenofovir release kinetics from hyaluronidase-sensitive nanomedicine: A deterministic approach","authors":"Coulibaly S. Fohona ,&nbsp;Vivek Agrahari ,&nbsp;Naveen K. Vaidya ,&nbsp;Bi-Botti C. Youan","doi":"10.1016/j.onano.2023.100167","DOIUrl":"10.1016/j.onano.2023.100167","url":null,"abstract":"<div><p>Despite being convenient and practical, current nanomedicine (NM) release kinetic models remain unscalable, non-specific and less descriptive of the underlying physicochemical determinants. However, a deterministic mathematical modelling could overcome these limitations. In this study, we develop a model, based on a system of two differential equations (accounting for nanoparticle (NP) degradation and then drug release from degraded NM), which enable us to estimate per capita rate constant α (#NP degraded/hr) and β (Drug Amount Released/NP), the net effect of the nanomedicine (NE factor ɣ= α.β) and the controlled release index (φ, ratio of drug release to NP degradation). The model analysis conducted with tenofovir loaded hyaluronidase sensitive NM clearly shows the α factor significantly increased with triggering stimuli due to its enzymatic action on its substrate (hyaluronic acid). However, the β factor remained relatively unchanged, due to intrinsic physicochemical properties of the drug as limiting factor. The application of the solutions of this model clearly enabled us to effectively screen among various nanoformulations and identified the best hyaluronidase-sensitive NM formulation, exhibiting the highest ratio (3.7-fold increase compared to no enzyme). The φ value confirmed the controlled release and stimuli sensitivity of the nanosystem. Moreover, the computed drug release rate (dM/dt) is consistent with other existing mathematical models (under valid assumption). The key advantages of this approach are i) relevancy to underlying physicochemical and biochemical process, ii) versatility and application to various NM kinetics, and iii) prediction of temporo-spatial distribution of the drug loaded NP that could potentially improve <em>in-vitro/in vivo</em> correlation study. This unique approach is applicable for a more specific and more meaningful/physicochemically relevant description of bioactive agents release from NM or NP for various applications.</p></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"13 ","pages":"Article 100167"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42037157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}