Preparation and Characterization of Alginate Nanocarriers as Mucoadhesive Intranasal Delivery Systems for Ameliorating Antibacterial Effect of Rutin Against Pasteurella Multocida Infection in Mice

Q2 Pharmacology, Toxicology and Pharmaceutics

OpenNano Pub Date : 2023-09-01 DOI:10.1016/j.onano.2023.100176

Amany M. Mohamed , Nagwa I. Toaleb , Ahmad Mohammad Allam , Sahar Hussein Abdalla Hekal , Sherein S. Abdelgayed , Abeer S. Hassan

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Abstract

Rutin is a natural product has various biological activities. Pasteurellosis is crucial bacterial infection of respiratory system caused by Pasteurella multocida. This study aimed to investigate the improved antibacterial effect of Rutin nanocarriers as mucoadhesive intranasal delivery against Pasteurella multocida. Different formulations of Rutin niosomes and nanostructure lipid carriers (NLCs) were formulated and well characterized. The in vivo antibacterial performance of the developed formulations against Pasteurella multocida in infected mice was conducted. Further, cytokines levels of Interferon Gamma (INF-γ) and Interlukin-12 (IL -12) in mice sera were assessed. The results revealed that developed Rutin nanocarriers were in nanosized range and exhibited high drug encapsulation. However, Rutin NLCs showed smaller particle size (240.34 ± 5.5 nm), higher encapsulation% (97.34 ± 0.15%), and higher drug release of 94.5% within 12 h comparing with Rutin niosomes. Further, Rutin NLCs presented the highest antibacterial effect against P. multocida infection compared with other treated groups. The bacterial count in lungs and livers was reduced in treated groups compared to the infected non treated one. Our results indicate that mucoadhesive Rutin nanocarriers introduce a new promising antibacterial agent for intranasal delivery against P. multocida and open vision for veterinary applications to utilize advanced nanocarriers in the management of several infections.

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海藻酸盐纳米载体的制备及其对芦丁抗多杀性巴氏杆菌感染的作用

芦丁是一种天然产物，具有多种生物活性。巴氏杆菌病是由多杀性巴氏杆菌引起的重要的呼吸系统细菌感染。本研究旨在探讨芦丁纳米载体作为黏附剂鼻内给药对多杀性巴氏杆菌的抗菌效果。制备了不同配方的芦丁乳小体和纳米结构脂质载体，并对其进行了表征。研究了所研制的制剂对多杀性巴氏杆菌感染小鼠的体内抗菌性能。进一步，评估小鼠血清中干扰素γ (INF-γ)和白细胞介素12 (IL -12)的细胞因子水平。结果表明，制备的芦丁纳米载体在纳米级范围内，具有较高的药物包封性。与芦丁niosomes相比，芦丁NLCs的粒径更小(240.34±5.5 nm)，包封率更高(97.34±0.15%)，12 h内释药率高达94.5%。此外，与其他处理组相比，芦丁NLCs对多杀假单胞菌感染的抗菌效果最高。与未接受治疗的感染组相比，治疗组肺部和肝脏中的细菌数量减少。我们的研究结果表明，黏附芦丁纳米载体为鼻内给药抗多杀双球菌提供了一种新的有前景的抗菌药物，并为利用先进的纳米载体治疗多种感染开辟了兽医应用的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

OpenNano Medicine-Pharmacology (medical)

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

50 days

期刊介绍： OpenNano is an internationally peer-reviewed and open access journal publishing high-quality review articles and original research papers on the burgeoning area of nanopharmaceutics and nanosized delivery systems for drugs, genes, and imaging agents. The Journal publishes basic, translational and clinical research as well as methodological papers and aims to bring together chemists, biochemists, cell biologists, material scientists, pharmaceutical scientists, pharmacologists, clinicians and all others working in this exciting and challenging area.