OpenNano最新文献

{"title":"Evaluation of Chitosan-Encapsulated Lemongrass (Cymbopogon citratus) Essential Oil Nanoemulsion for Fruit Edible Coating","authors":"Panji Setya Utama Putra ,&nbsp;Damar Rastri Adhika ,&nbsp;Gita Genecya ,&nbsp;Muhammad Samman Al Madanie ,&nbsp;Lia Amelia Tresna Wulan Asri","doi":"10.1016/j.onano.2025.100246","DOIUrl":"10.1016/j.onano.2025.100246","url":null,"abstract":"<div><div>Agricultural and fishery products generally have a limited shelf life since these products are at high risk of experiencing changes in sensory properties in a fairly short time due to oxidation, enzymatic reactions, and microbial activity. Therefore, a food preservation system is needed to maintain its quality for longer. One preservation method for post-harvest products such as fruits and vegetables is edible coating. Edible coatings can be developed using biodegradable polymers derived from polysaccharides, proteins, fats, and their derivatives. Chitosan is a type of biopolymer that is commonly derived from the exoskeletons of marine animals such as shrimp and it possesses good antimicrobial properties. In addition, the development of food packaging technology. This study aims to develop and evaluate edible coatings that contain nano-chitosan and lemongrass essential oil nanoemulsions as antioxidant and antimicrobial agents for fruit packaging. The synthesized lemongrass essential oil nanoemulsion size was less than 100 nm and had good stability with a below 10% size change during 56 days of storage. Lemongrass essential oil nanoemulsion had a high antioxidant activity, as indicated by the low IC₅₀ value in the DPPH reagent test. The combination of lemongrass essential oil nanoemulsion and chitosan as an edible coating had high antimicrobial activity, as indicated by the large inhibition zone in <em>Bacillus cereus</em> and <em>Escherichia coli</em> bacteria. Edible coating implementation on unpeeled banana fruit was evaluated through various tests such as pH, hardness, weight loss, and color changes. These tests showed some improvement in fruit preservation using nanoemulsion-based edible coatings treatment. The bananas were observed for 11 days and showed that the edible coating made from the combination of chitosan and lemongrass essential oil nanoemulsion can delay the ripening process of fruits, maintain firmness, and reduce weight loss. The best results for unpeeled banana preservation were obtained from edible coating with variations of lemongrass essential oil nanoemulsion and chitosan with a ratio of 2:1.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"24 ","pages":"Article 100246"},"PeriodicalIF":0.0,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Localized Periodontitis Treatment with Azithromycin-Loaded Niosomal Thermosensitive Gels","authors":"Kunchorn Kerdmanee ,&nbsp;Chuencheewit Thongsiri ,&nbsp;Nuttawut Supachawaroj ,&nbsp;Sucharat Limsitthichaikoon","doi":"10.1016/j.onano.2025.100247","DOIUrl":"10.1016/j.onano.2025.100247","url":null,"abstract":"<div><div>Periodontitis is a chronic inflammatory disease requiring localized drug delivery for sustained therapeutic action. Conventional treatments face challenges such as a rapid drug clearance and poor bioavailability. This study develops and evaluates an azithromycin-loaded niosomal thermoresponsive gel (AZG) to enhance drug retention and treatment efficacy. Azithromycin-loaded niosomes (AZN) were optimized using a 3² full factorial design with Span 60 and cholesterol, achieving nanoscale size, stable zeta potential, and high drug encapsulation. The optimized AZN was incorporated into a poloxamer 407-hyaluronic acid gel, ensuring in situ gelation and prolonged drug retention. AZG exhibited favorable gelation, biocompatibility, accelerated wound closure, enhanced cell proliferation, and significant anti-inflammatory effects (twofold IL-1β and sixfold TNF-α reduction, p &lt; 0.05). By integrating sustained drug release with antibacterial and anti-inflammatory properties, AZG presents a promising adjunctive therapy for periodontitis, addressing key limitations of existing treatments.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"24 ","pages":"Article 100247"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-cancer activity of Kaempferia galanga L.–loaded polydopamine nanoparticles against colorectal cancer","authors":"Paweena Dana ,&nbsp;Anukul Taweechaipaisankul ,&nbsp;Yodsathorn Wongngam ,&nbsp;Suttipun Sungsuwan ,&nbsp;Walailuk Chonniyom ,&nbsp;Saksorn Klibaim ,&nbsp;Prattana Tanyapanyachon ,&nbsp;Monthira Rattanatayarom ,&nbsp;Onuma Phoraksa ,&nbsp;Udom Asawapirom ,&nbsp;Primana Punnakitikashem ,&nbsp;Duangporn Polpanich ,&nbsp;Nattika Saengkrit","doi":"10.1016/j.onano.2025.100242","DOIUrl":"10.1016/j.onano.2025.100242","url":null,"abstract":"<div><div><em>Kaempferia galanga</em> L. (KGL) is an aromatic ginger that has been used as a medicinal plant. Specifically, KGL possesses anti-inflammatory, antioxidant, anti-bacterial, and anti-cancer effects. However, the key component of KGL, ethyl <em>p</em>‑methoxy cinnamate (EPMC), is insoluble in water, resulting in low bioavailability. Hence, a nano-drug delivery system is used to enhance KGL activities. This study aimed to employ polydopamine (PDA) nanoparticles as a carrier for KGL delivery to improve its anti-cancer activity against colorectal cancer cells. PDA- and PDA nanoparticle–loaded KGL (PDA-KGL) were synthesized using a spontaneous oxidation process. The physicochemical properties of the PDA-KGL were characterized by dynamic light scattering methods. The anti-cancer activity of PDA-KGL was evaluated in HT-29, a colorectal cancer (CRC) cell line. Average hydrodynamic sizes of PDA and PDA-KGL were 236.2 ± 1.2 and 316.6 ± 2.0 nm, respectively, and the zeta potential of PDA and PDA-KGL were -23.0 ± 0.4 and -39.5 ± 0.6 mV, respectively. The morphology of PDA-KGL observed under TEM was spherical in shape. Anti-proliferative activity was monitored in HT-29 cells using MTT and 3D tumor spheroid assays. PDA-KGL strongly inhibited cell viability of HT-29 cells compared to free KGL and PDA treatments. PDA-KGL induced apoptosis in HT-29 cells as shown by an Annexin V binding assay. In addition, PDA-KGL suppressed the invasive ability of HT-29 cells compared to free KGL or PDA, which was determined by a transwell invasion assay. Taken together, it implies that PDA-KGL might be used as a nano-drug delivery approach for colorectal cancer treatment.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"23 ","pages":"Article 100242"},"PeriodicalIF":0.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced immunomodulator activity of Carthamus tinctorius (L.) extracts, a traditional medicine using nanostructured lipid carrier approach","authors":"Rachmat Mauludin ,&nbsp;Insan Nurul Padjar ,&nbsp;Neng Fisheri Kurniati ,&nbsp;Ratna Annisa Utami","doi":"10.1016/j.onano.2025.100244","DOIUrl":"10.1016/j.onano.2025.100244","url":null,"abstract":"<div><div>Considering the potential of <em>Carthamus tinctorius (</em>L.<em>)</em> (CT) for therapeutic purposes mainly as immunomodulator as well as the ability of nanostructured lipid carriers (NLC) to protect phytochemicals from degradation and improving their solubility, the objective of this study was design, optimization, and characterization CT-loaded NLC (CT<img>NLC) system as an immunomodulator. The NLCs were prepared using hot homogenization techniques, which were later subjected to ultrasonication according to the Box Behnken design. The NLCs were characterized in terms of polydispersity index (PDI), particle size (PS), zeta potential (ZP), entrapment efficiency (%EE), and surface morphologies. The immunomodulatory activity was carried out by measuring phagocytic activity using carbon clearance method, organ index, antibody titres using indirect hemagglutination test and level of interferon-gamma (IFN-γ). The CT extracts (300 mg/kg), CT-NLC (100 mg/kg and 300 mg/kg) were administered orally. Levamisole (3 mg/kg) and Methylprednisolone (16 mg/kg) was used as standard drug. The selected CT<img>NLC displayed a spherical particle with an average of ∼199.6 nm, polydispersity index of ∼0.305 and zeta potential of ∼-27.4 mV. %EE ∼74.04 % was attained. TEM images exhibited nanoparticles to be non-aggregated and in spherical shape. CTNLC treatment significantly increased the phagocytic index, antibody titres and level of IFN-γ but did not influence organ index compared to free CT. <em>Carthamus tinctorius (</em>L.<em>)</em> was successfully encapsulated into nanostructured lipid carriers. The developed NLC exhibited desirable parameters and was found to further improve immunomodulation efficacy. NLC has the potential to significantly enhance the efficacy of CT.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"23 ","pages":"Article 100244"},"PeriodicalIF":0.0,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143697055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and evaluation of drug-loaded niosomes fabricated by flow chemistry: A novel vortex tube reactor approach","authors":"Kampanart Huanbutta ,&nbsp;Kittipat Suwanpitak ,&nbsp;Possawee Ponlakorn ,&nbsp;Phatsawi Sonkongdang ,&nbsp;Isariya Kongklai ,&nbsp;Kunlathida Luangpraditkun ,&nbsp;Tistaya Semangoen ,&nbsp;Tanikan Sangnim","doi":"10.1016/j.onano.2025.100243","DOIUrl":"10.1016/j.onano.2025.100243","url":null,"abstract":"<div><div>Continuous flow technology has been increasingly applied in the fabrication of nanoparticle drug delivery systems due to its ability to provide enhanced process control, scalability, and improved uniformity in particle size. Therefore, this study aims to utilize a newly designed flow chemistry vortex tube reactor for the preparation of ketoconazole-loaded niosomes, with the goal of enhancing mixing efficiency and increasing production rates. The experiment was designed using a central composite design to investigate the effects of key preparation parameters, including total flow rate, surfactant concentration, and cholesterol content, on particle size, size distribution, zeta potential, entrapment efficiency, and drug loading percentage. The optimized formulation (Span 80 = 25 mg, Cholesterol = 50 mg) was achieved using a total flow rate of 20 mL/min. The resulting niosomes exhibited a particle size of 212.3 nm, a zeta potential of 40.2 mV, a polydispersity index of 0.282, an entrapment efficiency of 50.84 %, a drug loading of 0.58 %, and a productivity of 70.67 mg/min. Moreover, ketoconazole-loaded niosomes prepared using the newly designed flow chemistry vortex tube reactor demonstrated prolonged inhibition of <em>Candida albicans</em> growth compared to ketoconazole solution. A comparison with batch synthesis revealed that flow chemistry produces smaller particles with a narrower size distribution and significantly improved productivity. These findings indicate the potential for further development of the vortex reactor for industrial-scale production of nanovesicular drug delivery systems.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"23 ","pages":"Article 100243"},"PeriodicalIF":0.0,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143724154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanotechnology for effective bovine mastitis treatment: Applications in biosensors and vaccines","authors":"Soheil Sadr ,&nbsp;Babak Khoramian ,&nbsp;Zahra Yousefsani ,&nbsp;Ahad Jafari Rahbar Alizadeh ,&nbsp;Abbas Rahdar ,&nbsp;Hassan Borji","doi":"10.1016/j.onano.2025.100238","DOIUrl":"10.1016/j.onano.2025.100238","url":null,"abstract":"<div><div>A dairy farm's most significant economic loss is caused by mastitis, one of the most common diseases in dairy cows. Mastitis occurs due to many different pathogens, such as bacteria, fungi, and algae, and antibiotics are the first line of treatment and control. Alternative methods are therefore being investigated to eradicate pathogenic microorganisms that cause mastitis. Nanotechnology is a rapidly growing field that can manufacture new materials at the nanoscale level and has tremendous potential to revolutionize the agri-food industry by offering new treatments for bovine mastitis. Considering the increasing ineffectiveness of current therapies for resistant bacteria, developing innovative products that combine nanotechnology may provide a sustainable animal health solution that can support the production of safe and high-quality food products while directly addressing a critical global problem such as antimicrobial resistance. Additionally, nanotechnology offers promising advancements in bovine mastitis research, with nanovaccines providing targeted and efficient delivery of antigens to enhance the cow's immune response and prevent the disease. On the other hand, nanobiosensors play a crucial role in the early detection of bovine mastitis, allowing for real-time monitoring of biomarkers and enabling prompt intervention, thus reducing economic losses and promoting animal welfare. Although metalic nanoparticles have the potential to offer great benefits in terms of treating bovine mastitis, there are several challenges related to their toxicity, stability, environmental safety, and production costs that need to be carefully examined and addressed in order to facilitate their use. Considering the potential of nanoparticles, these challenges can be successfully managed if applied to the right areas, which will be a new and efficient therapeutic method for improving bovine mastitis and reducing drug resistance in livestock farms, given the potential of nanoparticles. In summary, nanovaccines, nanodrugs, and nanobiosensors have great potential in managing bovine mastitis, offering targeted prevention, effective treatment, and early detection capabilities, respectively.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100238"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic selenium nanoparticles encapsulated Lactobacillus probiotics targeting colon tumorigenesis: A promising drug delivery system","authors":"Rehab M. Abdel-Megeed ,&nbsp;Shaymaa A. Ismail ,&nbsp;Mai O. Kadry","doi":"10.1016/j.onano.2025.100239","DOIUrl":"10.1016/j.onano.2025.100239","url":null,"abstract":"<div><div>Selenium nanoparticles are less toxic compared to other selenium forms, and their biogenic production leads to improve bioavailability. However, Lactobacillus probiotics previously exhibited anticancer activity. The current study was evaluated to investigate the therapeutic potential of biogenic selenium nanoparticles that was synthesized via <em>Lactbacillus plantarum (</em>L. <em>plantarum)</em> and <em>Lactbacillus casei (</em>L. <em>casei)</em> against colorectal cancer. The induction of colorectal cancer was carried out via a single dose (5 mg/kg) of 3-methyl-1, 2-dyhydrobenzo(j)aceanthrylene [3-MBA]. After that, animals were treated using 200 µl (from stock concentration 1.0 × 10⁹ CFUs per ml) of selenium nanoparticles derived from L. <em>casei</em> (SeNPs-Ca) or selenium nanoparticles derived from L. <em>plantarum</em> (SeNPs-P) as two separate treated groups for two weeks. Colorectal tumor induction declared a significant increment in total antioxidant capacity. Meanwhile, malondialdehyde level decreased upon 3-MBA intoxication. Molecular analysis of <em>Beclin1</em> as an autophagy signaling pathway revealed a remarkable reduction upon 3-MBA intoxication. Meanwhile, a significant overexpression was declared in both <em>EGFR</em> and <em>MMP-9</em> genes post 3-MBA-induced colorectal tumor. Furthermore, a remarkable alteration was recorded in the protein expression of both <em>p53</em> and <em>TNF-α</em> post 3-MBA injection. Both two treated groups of biogenic selenium nanoparticles declared a significant improvement in the measured parameters, with the superiority of SeNPs-Ca in modulating the altered biomarkers. Histopathological examination of colon tissue confirmed the obtained results. In conclusion, biogenic selenium nanoparticles derived from Lactobacillus probiotics could be a promising candidate as a safe and effective antitumor agent in colorectal tumor therapy and could be a promising drug delivery system targeting tumors.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100239"},"PeriodicalIF":0.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbicidal mechanisms for light-activated molecular nanomachines in Mycobacterium smegmatis: A model for pathogenic bacteria","authors":"Thushara Galbadage ,&nbsp;Dongdong Liu ,&nbsp;James M. Tour ,&nbsp;Jeffrey D. Cirillo ,&nbsp;Richard S. Gunasekera","doi":"10.1016/j.onano.2025.100240","DOIUrl":"10.1016/j.onano.2025.100240","url":null,"abstract":"<div><div>There is a global health crisis of antimicrobial resistance, responsible for over a million deaths annually. Mycobacterial infections are a major contributor to this crisis, causing more deaths than any other single infectious agent. Notably, the rise of multidrug-resistant (MDR), extensively drug-resistant (XDR), and totally drug-resistant (TDR) strains of <em>Mycobacterium tuberculosis</em> has led to higher mortality rates and challenge all existing antibiotic regimens. Light-activated molecular nanomachines (MNMs) represent a promising class of broad-spectrum antimicrobial agents that could help counter this rise in antimicrobial resistance. Addressing a key knowledge gap, this study explores the mechanisms of action for MNMs in <em>Mycobacterium smegmatis</em>, a surrogate model for pathogenic mycobacteria. We show that fast-rotor MNMs significantly reduce bacterial viability, achieving up to 97 % reduction in <em>M. smegmatis</em> with 30 minutes of light activation when compared to non-activated MNM <strong>1</strong> (<em>p</em> &lt; 0.0001, <em>t</em> = 24.55), as determined by an unpaired <em>t</em>-test. Using fluorescence and confocal microscopy, we also show the colocalization of MNM <strong>1</strong> with <em>M. smegmatis</em> as part of their mechanism of action. The ability to translate these observations to pathogenic mycobacteria was demonstrated by the ability of MNM <strong>1</strong> to kill 93.5 % of <em>M. tuberculosis</em> with 5 minutes of light activation when compared to non-activated MNM <strong>1</strong> (<em>p</em> &lt; 0.0001, <em>t</em> = 19.24). These findings suggest that MNMs have the potential to be innovative and sustainable antimicrobial agents for the treatment of pathogenic mycobacterial infections.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"23 ","pages":"Article 100240"},"PeriodicalIF":0.0,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoformulation, characterization, and biological activity assays of extracts of Derris trifoliata Lour, a rutin-rich mangrove plant","authors":"Prattana Tanyapanyachon,&nbsp;Walailuk Chonniyom,&nbsp;Kananat Naksomboon,&nbsp;Jakarwan Yostawonkul,&nbsp;Paweena Dana,&nbsp;Udom Asawapirom,&nbsp;Nattika Saengkrit","doi":"10.1016/j.onano.2025.100236","DOIUrl":"10.1016/j.onano.2025.100236","url":null,"abstract":"<div><div><em>Derris trifoliata</em> Lour (DT) is a mangrove plant species with a promising anti-inflammatory effect. However, the application of DT as a topical anti-inflammatory agent is limited due to its poor bioavailability. Here, DT extract (DTE) from Kapoe District, Ranong Province, Thailand was selected due to its highest rutin over three investigated sources. The DTE was loaded into a nanoemulsion (NE), giving 74.33 ± 0.12 % and 75.29 ± 0.51 %, encapsulation efficiency of rutin and total phenolic content (TPC), respectively. The DTE-loaded NE (DTE-NE) exhibited a nano size (90.59 ± 1.27 nm) spherical, negative charge (-33.13 ± 0.12 mV), and narrow dispersity index (0.28 ± 0.01). The <em>in vitro</em> release profile of rutin and TPC from DTE-NE was slower compared to those from DTE. DTE-NE displayed a superior antioxidant capacity to DTE. Compared to DTE, DTE-NE treatment potentially lowered the inflammatory mediators (nitrite, interleukin-6, and tumor necrosis factor-alpha). Moreover, the physicochemical characteristics, antioxidant and anti-inflammatory activities remained unchanged after 1 month storage. In conclusion, the present study demonstrated the possible use of DTE-NE as a topical anti-inflammatory product.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100236"},"PeriodicalIF":0.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resveratrol loaded zein nanoparticles for inhibiting proliferation of osteosarcoma cells: Synthesis, characterization, release profile, and cytotoxicity","authors":"Thanida Chuacharoen ,&nbsp;Carlos E. Astete ,&nbsp;Cristina M. Sabliov","doi":"10.1016/j.onano.2025.100237","DOIUrl":"10.1016/j.onano.2025.100237","url":null,"abstract":"<div><div>Resveratrol loaded zein nanoparticles (Res ZNPs) were synthesized to deliver resveratrol to bone cells for inhibiting the proliferation of osteosarcoma cells. Zein chemically cross-linked with alendronate as a bone-targeting agent using EDC and NHS chemistry was also synthesized and the cross-linking was confirmed using Fourier-Transform Infrared and Nuclear Magnetic Resonance spectroscopy. Subsequently, Res ZNPs with and without alendronate were synthesized and characterized. The particles measured 273 to 294 nm with a narrow polydispersity index, and a zeta potential of -29 to -33 mV, respectively as evaluated by dynamic light scattering. The particles showed spherical morphology imaged by transmission electron microscopy and the entrapment efficiency and loading capacity were 63.0 % and 13.6 % for Res ZNPs and 69.1 % and 21.0 % for Res ZNPs with alendronate, respectively. Furthermore, the entrapped resveratrol of both systems was released in a three-phase manner under physiological condition (phosphate-buffered saline, PBS) at 37 °C over 24 h. Both systems exhibited suppression of osteosarcoma MG-63 cell proliferation and the inhibition rate was found slightly higher for targeted, Res ZNPs with alendronate. This research suggested that Res ZNPs conjugated with alendronate could be a candidate for effective bone-targeting delivery systems to inhibit the proliferation of osteosarcoma cells.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"22 ","pages":"Article 100237"},"PeriodicalIF":0.0,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143386463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}