OpenNano Pub Date : 2026-05-01 Epub Date: 2026-04-28 DOI: 10.1016/j.onano.2026.100305

Valentina Pacciani , Deborah Bonavolontà , Jacopo Cardellini , Lucrezia Caselli , Arianna Balestri , Debora Berti

{"title":"Engineering organic-organic and organic-inorganic nanohybrids for Drug Delivery","authors":"Valentina Pacciani , Deborah Bonavolontà , Jacopo Cardellini , Lucrezia Caselli , Arianna Balestri , Debora Berti","doi":"10.1016/j.onano.2026.100305","DOIUrl":"10.1016/j.onano.2026.100305","url":null,"abstract":"<div><div>Hybrid nanoparticles (HNPs) are emerging as cutting-edge materials in nanomedicine, offering unique opportunities for drug delivery, diagnostic imaging, and therapeutics. By harnessing interactions between organic or inorganic components, these systems achieve precise control over size, surface, and functional properties. The synergy between different building blocks enables enhanced biocompatibility, targeted delivery, and stimuli-responsive functions that are challenging to achieve with single-component systems. This review highlights the state of the art in organic–organic and organic–inorganic nanohybrids, focusing on recent advances for drug delivery applications. Examples include organic-organic hybrids combining lipids, polymers, and biological constituents, <em>e.g.</em>, extracellular vesicle- or cell membranes-derived components, as well as inorganic NPs hybridized with polymers, peptides, or lipids. We further discuss current challenges and future perspectives in the field, including hybrids that are responsive to internal or external triggers, which hold significant promise for advancing personalized medicine and translating nanomedicine innovations into the clinic.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"29 ","pages":"Article 100305"},"PeriodicalIF":0.0,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147802301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flexible asymmetric liposomes as a platform for improved plasmid DNA delivery: An in vitro study 柔性不对称脂质体作为改进质粒DNA传递的平台：一项体外研究

OpenNano Pub Date : 2026-03-01 Epub Date: 2026-01-27 DOI: 10.1016/j.onano.2026.100283

Denisse Gardea-Gutiérrez , Manuel Román-Aguirre , Raúl Loera-Valencia , Blanca Sánchez-Ramírez , Yocanxóchitl Perfecto-Avalos , Silvia L. Montes-Fonseca

{"title":"Flexible asymmetric liposomes as a platform for improved plasmid DNA delivery: An in vitro study","authors":"Denisse Gardea-Gutiérrez , Manuel Román-Aguirre , Raúl Loera-Valencia , Blanca Sánchez-Ramírez , Yocanxóchitl Perfecto-Avalos , Silvia L. Montes-Fonseca","doi":"10.1016/j.onano.2026.100283","DOIUrl":"10.1016/j.onano.2026.100283","url":null,"abstract":"<div><div>As a versatile type of nanocarrier, liposomes have gained attention due to their biocompatibility, biodegradability, and ability to encapsulate a wide range of compounds. Asymmetric liposomes are characterized by distinct lipid compositions in their inner and outer layers, enhancing drug encapsulation and stability. Surfactant compounds and ethanol confer the flexibility of liposomes in their lipid layer. This characteristic allows liposomes to deform without breaking or releasing their contents, enabling them to cross complex barriers, such as the skin. This research investigates the innovative design and application of flexible asymmetric liposomes for transdermal DNA delivery. By using an adapted inverse emulsion technique, this study successfully encapsulated plasmid DNA within flexible asymmetric liposomes to evaluate its biocompatibility and skin permeation capacity in an in vitro model. Several formulations of liposomes containing DOTMA, DOPE, and/or cholesterol as the inner layer and DSPC, Span80, and/or ethanol as the outer layer were assessed through viability assay, transfection efficiency, and permeability testing using the Parallel Artificial Membrane Permeability Assay (PAMPA). All formulations demonstrated over 69% cell viability at a concentration of 5 μg, and transfection efficiency was significantly enhanced, reaching transfection rates of 32.37% ±3.32% in liposomes with all components. Permeability testing showed that the liposomal formulations exhibited high permeability, further improved by including ethanol and surfactants. Key findings indicate that cholesterol, Span 80, and ethanol substantially contribute to transfection rates and permeation. This research underscores the potential of flexible asymmetric liposomes for effective transdermal delivery of genetic material.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"28 ","pages":"Article 100283"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146078862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microalgae-derived biomolecule and nanoparticle conjugates: Emerging therapeutic platforms for cancer treatment 微藻衍生的生物分子和纳米颗粒缀合物：癌症治疗的新兴治疗平台

OpenNano Pub Date : 2026-03-01 Epub Date: 2026-02-23 DOI: 10.1016/j.onano.2026.100291

Ozge Cinar, Ilgin Kimiz-Gebologlu, Sevval Kurt, Suphi S. Oncel

{"title":"Microalgae-derived biomolecule and nanoparticle conjugates: Emerging therapeutic platforms for cancer treatment","authors":"Ozge Cinar, Ilgin Kimiz-Gebologlu, Sevval Kurt, Suphi S. Oncel","doi":"10.1016/j.onano.2026.100291","DOIUrl":"10.1016/j.onano.2026.100291","url":null,"abstract":"<div><div>Nanoparticle-based drug delivery systems have the potential to provide a promising platform for overcoming the limitations of therapeutics in cancer treatment, thanks to advantages such as controlled release, tumor targeting, and reduced systemic toxicity. In parallel, microalgal biomolecules possess strong antioxidant and antiproliferative properties. However, these biomolecules are therapeutically limited due to their low stability and restricted bioavailability. The approach of using microalgal compounds in combination with nanoparticles both improves their pharmacokinetic properties and enhances multiple anticancer mechanisms (ROS modulation, caspase activation, etc.). This review focuses on the anticancer potential of microalgal biomolecules and nanoparticulate systems, the intracellular mechanisms of action of the conjugated platforms they form, and their biocompatibility advantages. It also describes the potential of conjugated platforms for future clinical applications, highlighting their importance as biologically synergistic and targeted next-generation therapeutic platforms in cancer treatment.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"28 ","pages":"Article 100291"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147397606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enantiomeric lysine-based cationic lipids: Design, synthesis, and characterization for in vitro gene delivery 基于赖氨酸的阳离子脂质对映体：设计、合成和表征体外基因传递

OpenNano Pub Date : 2026-03-01 Epub Date: 2025-12-28 DOI: 10.1016/j.onano.2025.100280

Berenice Erendira Oseguera-Guerra , Oliver Lopez-Villegas , Manuel Román-Aguirre , Alfredo Aguilar-Elguezabal , Raúl Loera-Valencia , Silvia Lorena Montes-Fonseca

{"title":"Enantiomeric lysine-based cationic lipids: Design, synthesis, and characterization for in vitro gene delivery","authors":"Berenice Erendira Oseguera-Guerra , Oliver Lopez-Villegas , Manuel Román-Aguirre , Alfredo Aguilar-Elguezabal , Raúl Loera-Valencia , Silvia Lorena Montes-Fonseca","doi":"10.1016/j.onano.2025.100280","DOIUrl":"10.1016/j.onano.2025.100280","url":null,"abstract":"<div><div>Nucleic acid delivery is crucial for gene therapy, vaccination, and cancer treatment. Despite advances in cationic lipid-based vectors, their transfection efficiency is often limited by structural complexity. In this study, we synthesized lysine-based cationic lipids by esterifying <span>d</span>- or <span>l</span>-lysine with alcohols of varying chain lengths. The compounds were characterized by infrared spectroscopy and mass spectrometry, incorporated into liposomes, and evaluated for transfection efficiency, particle size, morphology, and cytotoxicity <em>in vitro.</em> Transfection performance increased with chain length, peaking at 20 carbons, with no significant differences between <span>d</span>- and <span>l</span>-enantiomers. Compared to Lipofectamine 2000, the optimized liposomes showed superior gene delivery while preserving cell viability. They displayed predominantly spherical to oval morphologies, particle sizes of 30–130 nm, and negligible cytotoxicity. These results suggest lysine-based cationic lipids are promising, safe, and effective nonviral vectors for nucleic acid delivery.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"28 ","pages":"Article 100280"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145904056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obesity-associated pathologies: Recent advances in stimuli-responsive nanocarriers for adipose tissue browning and beyond 肥胖相关病理：脂肪组织褐变及其他刺激反应性纳米载体的最新进展

OpenNano Pub Date : 2026-03-01 Epub Date: 2026-02-17 DOI: 10.1016/j.onano.2026.100290

Asmaa M. Elsherbini, Shaymaa A. Mohamed, Aya M. Zayed, Wafaa A. Mohamed, Sally A. Sabra

{"title":"Obesity-associated pathologies: Recent advances in stimuli-responsive nanocarriers for adipose tissue browning and beyond","authors":"Asmaa M. Elsherbini, Shaymaa A. Mohamed, Aya M. Zayed, Wafaa A. Mohamed, Sally A. Sabra","doi":"10.1016/j.onano.2026.100290","DOIUrl":"10.1016/j.onano.2026.100290","url":null,"abstract":"<div><div>Obesity is a chronic multifactorial disorder affecting more than 600 million people worldwide. It is initially caused by an imbalance between energy intake and consumption, and it is strongly correlated to many chronic and life-threatening disorders such as cardiovascular diseases, digestive diseases, colorectal cancer, and others. Obesity can be primarily managed via changing caloric consumption and including physical activity in the daily routine. Another solution is pharmacological therapies such as appetite suppressors and glucagon-like peptide-1 receptor agonists. Surgical intervention is also an available solution, but it is invasive, whereas changing caloric consumption or physical activity usually affords poor outcomes. Browning agents can convert WATs (white adipose tissues, energy-storing cells) into BATs (brown adipose tissues, energy-expending cells), which is an effective approach to reduce body weight. However, most of the available browning agents, either synthetic or natural, suffer from several drawbacks ranging from poor aqueous solubility to severe cardiovascular toxicity. As a result, more innovative approaches can be utilized, which can target obesity, such as passively targeted nanocarriers, depending on their lipophilicity or cationic nature. Actively targeted nanocarriers can target obesity depending on endothelial, adipocyte, or macrophage-based targeting. However, most recent research has transferred to develop more precise stimuli-responsive nanocarriers that can provide an on-demand drug release in response to different stimuli, either intrinsic or extrinsic. In this review, the most recent advances in stimuli-responsive nanocarriers in managing obesity and some of its related disorders including cardiovascular diseases and colorectal cancer, will be discussed.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"28 ","pages":"Article 100290"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147397605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Scaling up nanoformulation manufacturing: A multi–case study linking target product profiles, critical quality attributes, and quality by design 扩大纳米制剂制造：多案例研究链接目标产品概况，关键质量属性和质量的设计

OpenNano Pub Date : 2026-03-01 Epub Date: 2026-02-11 DOI: 10.1016/j.onano.2026.100288

Shriraj B. Patel, Dhanashree P. Sanap

{"title":"Scaling up nanoformulation manufacturing: A multi–case study linking target product profiles, critical quality attributes, and quality by design","authors":"Shriraj B. Patel, Dhanashree P. Sanap","doi":"10.1016/j.onano.2026.100288","DOIUrl":"10.1016/j.onano.2026.100288","url":null,"abstract":"<div><div>Nanoformulation platforms have transformed drug delivery; however, reliable scale-up from laboratory to manufacturing remains the principal barrier to clinical translation. This review integrates target product profile (TPP)–driven requirements with critical quality attributes (CQAs), platform-specific unit operations, and Quality by Design (QbD) principles to analyze scalable manufacturing of five major nanoformulation classes—lipid/liposome, polymeric/micellar, nanoemulsion, nanocrystal, and albumin/biopolymer systems—which collectively represent over 80 % of clinically approved nanomedicines. Quantitative analysis across these platforms demonstrates that controlled micromixing and solvent displacement routinely yield lipid and polymeric nanoparticles in the 50–200 nm range, while energy density–constrained high-pressure homogenization governs droplet size distributions in nanoemulsions, and stress intensity and stabilizer adsorption dictate nanocrystal quality. Protein-based carriers are shown to be particularly sensitive to raw-material variability and crosslinking kinetics. Five industrial case studies (Doxil®/CAELYX®, Onpattro®, Comirnaty®, Abraxane®, and AmBisome®) illustrate how orthogonal analytics (e.g., DLS and AF4–MALS), closed single-use architectures, and digitally enabled QbD–PAT frameworks link critical process parameters (CPPs) to robust control strategies across development and commercial manufacture. Overall, the review highlights standardization, quantitative comparability, and data-driven control as central enablers of scalable and regulatory-ready nanomedicine manufacturing.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"28 ","pages":"Article 100288"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147397603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formulation, characterization, and valorization of the antioxidant, antibiofilm, and anti-adhesion potentials of a clove essential oil nanoemulsion 丁香精油纳米乳的抗氧化、抗生物膜和抗粘附潜能的配方、表征和评价

OpenNano Pub Date : 2026-03-01 Epub Date: 2026-02-07 DOI: 10.1016/j.onano.2026.100286

Enis Ben Bnina , Jihen Missaoui , Mohamed Firas Gannouni , Lotfi Achour , Badr Bahloul

{"title":"Formulation, characterization, and valorization of the antioxidant, antibiofilm, and anti-adhesion potentials of a clove essential oil nanoemulsion","authors":"Enis Ben Bnina , Jihen Missaoui , Mohamed Firas Gannouni , Lotfi Achour , Badr Bahloul","doi":"10.1016/j.onano.2026.100286","DOIUrl":"10.1016/j.onano.2026.100286","url":null,"abstract":"<div><div>Food safety and control are fundamental public health issues that maintain consumer confidence and protection. This design (management) requires monitoring production, processing, and conservation processes while limiting microbiological, chemical, and physical risks. In this context, clove essential oil, a bioactive essence with a broad spectrum of biological activities, is increasingly studied as a natural biopreservative in innovative food safety strategies. This study reports the encapsulation of this oil into a nanoemulsion at concentrations of 10 mg/mL, 1 mg/mL, and 0.1 mg/mL. Dynamic light scattering, zeta potential, polydispersity index, and particle size all demonstrated optimal morphology and a stable system.</div><div>The nanoformulation significantly improves the antioxidant activity of clove essential oil, even at high dilutions, while protecting the degradation of natural active ingredients and ensuring controlled release. This nanoemulsion exhibits a moderate antimicrobial effect against various resistant pathogenic bacteria and fungi. However, at a concentration of 10 mg/mL, it showed a 100 % antibiofilm effect against both Gram-positive and Gram-negative bacteria. This nanoemulsion demonstrated significant potential to prevent surface adhesion against all bacteria tested, except <em>L. monocytogenes</em>.</div><div>This research reveals crucial information about an innovative natural nanoemulsion for food safety and control, unveiling its antioxidant, anti-adhesive, and anti-biofilm potential against resistant pathogens. These results represent a promising step forward in reducing contamination risks and extending shelf life without altering organoleptic properties, offering environmental benefits and hygiene practices while targeting sustainable industrial applications to increase food safety.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"28 ","pages":"Article 100286"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147397763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and in vitro evaluation of self-emulsifying drug delivery systems liquisolid lozenges of ibuprofen via design of experiments for solubility enhancement and oral epithelial cell permeation 布洛芬液体含片自乳化给药系统的开发及体外评价，通过设计溶解度增强和口腔上皮细胞渗透的实验

OpenNano Pub Date : 2026-03-01 Epub Date: 2026-01-11 DOI: 10.1016/j.onano.2026.100281

Sukannika Tubtimsri, Poonsak Charoensak, Apichaya Sukontachard, Yotsanan Weerapol

{"title":"Development and in vitro evaluation of self-emulsifying drug delivery systems liquisolid lozenges of ibuprofen via design of experiments for solubility enhancement and oral epithelial cell permeation","authors":"Sukannika Tubtimsri, Poonsak Charoensak, Apichaya Sukontachard, Yotsanan Weerapol","doi":"10.1016/j.onano.2026.100281","DOIUrl":"10.1016/j.onano.2026.100281","url":null,"abstract":"<div><div>Ibuprofen lozenges were developed by converting liquid self-emulsifying drug delivery systems (L-SEDDS) into liquisolid compacts (LSCs) to enhance aqueous solubility, support local anti-inflammatory action in the oral and throat regions, and reduce gastrointestinal irritation. The solubility of ibuprofen in oils and nonionic surfactants was evaluated. Tween 60 showed the highest solubility (285.53 ± 19.31 mg/g), whereas Imwitor® 742 (135.12 ± 0.21 mg/g) provided a suitable oil phase. L-SEDDS composed of ibuprofen, Tween 60, and Imwitor® 742 (9:1, w/w) formed nanoemulsions in simulated salivary fluid (SSF) with droplet sizes of approximately 200 nm. The L-SEDDS was loaded onto polyvinylpyrrolidone, magnesium aluminosilicate, sucrose, or F-Melt® C (FMC) to obtain LSC lozenges. A three-factor Box–Behnken design was employed to investigate the effects of L-SEDDS level, PVP content, and diluent type on tablet hardness and mean dissolution time (MDT). Suitable sucrose-LSC (Run7) and FMC-LSC (Run13) formulations showed rapid dissolution in SSF, with MDT values of 6.59 ± 0.15 and 5.91 ± 0.13 min, respectively, compared with 45.34 ± 1.11 min for ibuprofen powder. In a KON cell monolayer model, L-SEDDS and FMC-LSC (Run13) increased the apparent permeability coefficient of ibuprofen by approximately 3.6- and 3.5-fold, respectively, compared with ibuprofen powder. Both formulations maintained ibuprofen content, droplet size, dissolution behavior, and mechanical strength after 6 months of storage under both ambient and accelerated conditions. These results indicate that L-SEDDS-based LSC lozenges, particularly the FMC formulation, provide a stable dosage form that improves dissolution and mucosal permeation of poorly water-soluble ibuprofen.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"28 ","pages":"Article 100281"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145979783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spacer-engineered carboxylated curdlan derivatives for dendritic cell activation and cancer immunotherapy 用于树突状细胞活化和癌症免疫治疗的间隔器工程羧化凝乳蛋白衍生物

OpenNano Pub Date : 2026-03-01 Epub Date: 2026-02-13 DOI: 10.1016/j.onano.2026.100289

Xin Shu , Eiji Yuba

{"title":"Spacer-engineered carboxylated curdlan derivatives for dendritic cell activation and cancer immunotherapy","authors":"Xin Shu , Eiji Yuba","doi":"10.1016/j.onano.2026.100289","DOIUrl":"10.1016/j.onano.2026.100289","url":null,"abstract":"<div><div>Effective cancer immunotherapy requires cytosolic delivery of antigens into dendritic cells together with their maturation to elicit robust cellular immunity. Conventional vaccine carriers often combine multiple components to achieve both adjuvant activity and antigen delivery, which increases formulation complexity and raises safety concerns. Here, we present a strategy to integrate these functions into a single material by synthesizing carboxylated curdlan derivatives with tailored spacer structures. Introducing carboxy groups into curdlan, a representative polysaccharide adjuvant, conferred pH-sensitivity and improved water solubility, while spacer hydrophobicity modulated protonation behavior and interactions with lipid membranes. Among the derivatives, Chex-Curd, bearing a cyclohexyl spacer within carboxylated units, exhibited strong adjuvant activity and enhanced antigen presentation <em>via</em> the MHC class I pathway, which might result from cytosolic delivery of antigen. Chex-Curd also induced high levels of TNF-α and IL-12 secretion and upregulated dendritic cell maturation markers. Conjugates of Chex-Curd with a model antigenic protein elicited strong CD8⁺ T-cell responses and achieved significant tumor growth suppression in a mouse tumor model. These findings demonstrate that spacer engineering of polysaccharide-based adjuvants enables simultaneous dendritic cell activation and cytosolic antigen delivery, providing a promising platform for next-generation cancer vaccines.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"28 ","pages":"Article 100289"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147397761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of new delivery systems of hyaluronic acid based on silica hydrogels: In vitro kinetics of release and enzymatic degradation of the drug 基于二氧化硅水凝胶的透明质酸新递送系统的开发：药物的体外释放动力学和酶降解

OpenNano Pub Date : 2026-03-01 Epub Date: 2025-12-27 DOI: 10.1016/j.onano.2025.100279

Ekaterina Dolinina, Elena Parfenyuk

{"title":"Development of new delivery systems of hyaluronic acid based on silica hydrogels: In vitro kinetics of release and enzymatic degradation of the drug","authors":"Ekaterina Dolinina, Elena Parfenyuk","doi":"10.1016/j.onano.2025.100279","DOIUrl":"10.1016/j.onano.2025.100279","url":null,"abstract":"<div><div>Hyaluronic acid (HA) is a well-known anti-inflammatory drug that is widely used clinically to treat various diseases and as a component in cosmetic products. However some properties of HA (rapid release and enzymatic degradation) significantly reduce its functioning in the body and, consequently, its therapeutic effect. To develop new soft delivery system of HA with improved functional properties, the drug was encapsulated into silica hydrogels with various surface chemistry of silica matrix. The hydrogels of silica modified with aminopropyl and mercaptopropyl groups, as well as unmodified silica were studied as HA carriers. The effects of silica matrix modification, average molecular weight and concentration HA on the kinetics of its release from the formed composite hydrogels, as well as enzymatic degradation were revealed in vitro. It was found that the encapsulation of HA into silica hydrogels significantly slows down release process of the acid and, in most cases, its enzyme-induced degradation. The release controlled by delayed (pseudo-Fickian) diffusion as well as the protective effect of the silica hydrogel matrixes on the enzymatic degradation showed that the silica hydrogels are promising materials for creation of new soft formulations of HA.</div></div>","PeriodicalId":37785,"journal":{"name":"OpenNano","volume":"28 ","pages":"Article 100279"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145979782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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