Biomedical Photonics最新文献

{"title":"Photodynamic therapy in the treatment of HPV-associated cervical cancer: mechanisms, challenges and future prospects","authors":"N. А. Shanazarov, S. V. Zinchenko, S. D. Kisikova, A. A. Rizvanov, S. Smailova, K. A. Petukhov, Zh. Zh. Salmaganbetova","doi":"10.24931/2413-9432-2023-13-1-47-55","DOIUrl":"https://doi.org/10.24931/2413-9432-2023-13-1-47-55","url":null,"abstract":"Photodynamic therapy (PDT) has shown promise as a modality for the treatment of cervical cancer caused by the human papillomavirus (HPV). This review provides a comprehensive examination of the role of PDT in overcoming the challenges presented by conventional treatments for cervical cancer. Beginning with an overview of the relationship between cervical cancer and HPV infection, the review introduces the principles of PDT, its mechanism of action, and its potential as an innovative treatment strategy. The review highlights preclinical studies in animal models that demonstrate the efficacy of PDT in targeting HPV-infected cervical cells and provide mechanistic insights into its cytotoxic effects. We reviewed clinical studies and case reports highlighting the potential of PDT as an alternative or adjunctive treatment option. Challenges and limitations, including depth of light penetration, photosensitizer specificity, and standardization of protocols, will be discussed in the context of potential side effects and comparison with conventional treatments. Future directions include ongoing research, combination therapies with immunotherapy or targeted agents, advances in photosensitizer development, and personalized approaches. The advancement of PDT promises to change the landscape of HPV-associated cervical cancer treatment by providing a targeted, personalized, and minimally invasive approach.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"66 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140964876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photodynamic therapy of leukoplakia of the oral mucosa: experience of using method in 223 patients","authors":"T. Artsemyeva, D. Tzerkovsky","doi":"10.24931/2413-9432-2023-13-1-39-46","DOIUrl":"https://doi.org/10.24931/2413-9432-2023-13-1-39-46","url":null,"abstract":"The aim of this study was to analyze the immediate and long-term results of using photodynamic therapy (PDT) in patients with leukoplakia of the oral mucosa. The retrospective study included 223 patients with morphologically verified leukoplakia of the oral mucosa. Patients received treatment at the hyperthermia and photodynamic therapy department from 2013 to 2023. The average age was 51.9±8.9 years. Clinically, 211 patients (94.6%) had a flat form of the disease, 12 patients (5.4%) had a verrucous form. The photosensitizer (PS), based on chlorin e6, was administered intravenously once in doses of 1.7 to 2.5 mg/kg body weight. Irradiation of pathologically changed lesions was carried out 2-2.5 hours after the end of its infusion, using a semiconductor laser device “UPL PDT”, with a wavelength λ=665±5 nm. The laser radiation dose density varied from 25 to 100 J/cm², the power density from 0.07 to 0.32 W/cm², the duration of one irradiation field - from 2 to 13.5 minutes, depending on its linear dimensions. The severity of adverse reactions was assessed on days 1-30 after treatment using the CTCAE 4.03 scale. The immediate results of treatment were assessed up to 3 months after PDT, with morphological confirmation of the response to treatment. No serious adverse reactions were observed during PS infusion and irradiation. No symptoms of dermal phototoxicity were reported. During control examinations, up to 3 months after irradiation in patients with flat and verrucous forms, the frequency of complete regressions was 97.1% (n=205) and 58.3% (n=6) cases, partial regressions - 2,9% (n=7) and 25% (n=3) of cases, respectively. Objective response rates were observed at 100% and 83.3%, respectively. The period of observation of patients varies from 3 to 120 months. (average 66 months). The frequency of disease relapses during this period was 9%. Patients with partial regression and identified relapse were treated with repeated PDT sessions. PDT is a well-tolerated and effective treatment method for patients with leukoplakia of the oral mucosa, which has significant advantages over traditional methods of treating this precancerous pathology. These include minimal toxicity to the normal tissues surrounding the pathological foci due to the selective accumulation of PS in leukoplakia tissues, a slight risk of serious adverse reactions, the possibility of an outpatient session, the possibility of repeated treatment over a large area of damage and good cosmetic results).","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"15 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140968926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photodynamic therapy in treatment of squamous cell carcinoma of oral cavity with chlorine e6 photosensitizer with long-term follow up","authors":"Yury Panaseykin, V. Kapinus, E. Filonenko, V. Polkin, F. Sevrukov, M. A. Smirnova, P. Isaev, S. A. Ivanov, A. D. Kaprin","doi":"10.24931/2413-9432-2023-13-1-28-38","DOIUrl":"https://doi.org/10.24931/2413-9432-2023-13-1-28-38","url":null,"abstract":"Photodynamic therapy is an effective method for treating superficial forms of malignant neoplasms, characterized by a minimal risk of damage to normal tissues. In this study, we presented our experience of treating cancer of the oral mucosa using photodynamic therapy, and analyzed the immediate and long-term results of treatment. 38 patients with squamous cell carcinoma of oral cavity mucosa, with a depth of invasion no more than 7 mm, were included in the study. All patients underwent photodynamic therapy with chlorine e6 based photosensitizer. Photosensitizers were administered intravenously 3 hours before irradiation, at a dosage of 1 mg/kg of the patient’s weight. Photodynamic therapy was performed with the following parameters: P – 1.0 W, Ps – 0.31 W/cm2, E – 300 J/cm2. The area of one irradiation field ranged 1.0-2.0 cm2. Treatment effect was evaluated by RECIST 1.1. Overall survival, cancer-specific survival, and disease-free survival were calculated using Kaplan-Meier curves. Evaluation of adverse events was made by .TCAE 5.0 criteria. At 35 (92.1%) out of 38 cases, complete regression was observed after photodynamic therapy. Among them in 3 out of 35 patients relapse was diagnosed in 11.5 to 43.2 months. The total number of patients who didn’t respond to treatment was 6 (15.8%). Follow-up period was 4.2-87.3 months. (mean 42.9). 34 (89.5%) out of 38 patients are alive, 1 (2.6%) died from progression, and three died from other causes. The 5-year overall survival rate was 82.1%, cancer-specific survival rate was 97.0%, and disease-free survival rate was 81.1%. Among the factors significantly (p < 0.05) influencing relapse-free survival: depth of invasion < 5 mm (p – 0.013) and the presence of leukoplakia (p – 0.007). When assessing cancer-specific survival, factors worsening the prognosis were: age >70 years (p – 0.034) and the presence of leukoplakia (p – 0.007). Photodynamic therapy is an alternative treatment method of oral cancer superficial lesions, in case of proper assessment of primary lesion and in case of possibility of full irradiation of the tumor. Moreover, after using photodynamic therapy, the underlying connective-muscular structures are preserved, which promotes rapid healing with minimal scarring, the functions of the affected organ remain intact, and cosmetic defects do not form.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":" 58","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141128257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of silver nanoparticle and low-level laser on the immune response and healing of albino mice skin wounds","authors":"H. H. Soltan, A. Afifi, A. Mahmoud, M. Refaat, O. F. Al Balah","doi":"10.24931/2413-9432-2023-13-1-16-27","DOIUrl":"https://doi.org/10.24931/2413-9432-2023-13-1-16-27","url":null,"abstract":"The structural integrity of the skin, which acts as a barrier to keep harmful external substances out of the body, is compromised by wounds. The process of wound healing is a multifaceted and ever-changing phenomenon that entails the replacement of bodily tissues or damaged skin. It has been demonstrated that nanoparticles, especially silver nanoparticles, have anti-microbial and anti-inflammatory qualities that encourage cell migration and proliferation. Low level laser therapy has the potential to accelerate wound healing by stimulating cell regeneration after injury, reducing pain, and modulating the immune system. The aim of our study is to evaluate the healing process after treatment with silver nanoparticle and/or low level laser by measuring the serum levels of some pro-inflammatory cytokines (IL1b, IL6, and TNF-α), wound healing rate and histological analysis. Wounds were inflicted into 63 adult male albino mice (Mus musculus) and randomly divided into nine groups (7 per each). Control was left to normal healing. Other groups received a different treatment with laser, silver nanoparticle or both for 21 days. Injured skin was sampled for histopathological examination. Quantitative determination of TNFα, IL1 beta and IL6 were carried out using the sandwich enzyme-linked immunosorbent assay (ELISA) twice (day 2 and day 21). One-way and two-way analysis of variance (ANOVA) was used for statistical analysis. The results showed that the groups treated with silver nanoparticles and / or low-level laser promoted wound healing by reducing pro- inflammatory cytokines (IL1β, IL6 and TNFα) and showed significantly better wound closure with a significant reduction in wound size. At day 2 histopathological changes were very similar in different groups. When silver nanoparticles were applied, either alone or in combination with laser exposure, better granulation tissue and fibrosis also necrosis in the canter of the lesion and high score of re-epithelialization with less inflammation observed gradually till day 21. The results of this study suggested that silver nanoparticles and low-level laser have a wound healing potential, since topical treatment with silver nanoparticles and low-level leasers has effectively improved the wound healing process.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"117 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140987805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of 650 nm red laser photobiomodulation therapy to accelerate wound healing post tooth extraction","authors":"S. D. Astuti, R. Nashichah, P. Widiyanti, E. M. Setiawatie, M. S. Amir, A. Apsari, .. Widyastuti, E. Hermanto, Y. Susilo, A. K. Yaqubi, D.Z. I. Nurdin, N. Anuar","doi":"10.24931/2413-9432-2024-13-1-4-15","DOIUrl":"https://doi.org/10.24931/2413-9432-2024-13-1-4-15","url":null,"abstract":"After tooth extraction, there can be consequences involving injury to the tissue surrounding the extracted tooth, which may lead to severe problems such as inflammation and infection. The wound healing process comprises inflammation, proliferation, and remodeling phases. Photobiomodulation is a therapy form that utilizes the interaction of a light source with tissue. This interaction can activate an increase in Adenosine Triphosphate (ATP), which subsequently triggers a chain reaction leading to the creation of new blood vessels and an increase in the number of fibroblasts. This study used a red laser light source with a power of 3.32 ± 0.01 mW, delivering a dose of 3.5 J to patients for extraction indications. The parameters observed included Interleukin 1_ (IL-1_), Prostaglandin E2 (PGE2), Human Beta defensin 2 (HBD2), and Gingival Index (GI). The results of testing saliva samples using the enzyme-linked immunosorbent test (ELISA) for the parameters IL-1_, PGE2, and HBD2 show a significant influence between the control and therapy groups. Meanwhile, GI revealed a significant influence of therapy on the wound-healing process. Using the Mann-Whitney U test, on day 1, the p-value was found to be 0.32, indicating no significant deference between the control and therapy groups. However, on the third day after the therapy was administered, the p-value was obtained as 0.01, signifying a significant deference between the control and therapy groups. On day 5, a p-value of 0.034 was obtained, signifying a significant deference between the control and therapy groups. Based on the research results, it can be observed that there is a decrease in the values of IL-1_, PGE2, HBD2, and GI. This indicates that local immune cells, including resident macrophages, are activated by pro-inflammatory mediators released in response to injury, and they play an essential role in accelerating wound healing.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":" 31","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140991728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photodynamic therapy for a patient with basal cell skin cancer of the lower eyelid (clinical case)","authors":"E. Filonenko, N. I. Grigoryevykh","doi":"10.24931/2413-9432-2023-12-4-30-32","DOIUrl":"https://doi.org/10.24931/2413-9432-2023-12-4-30-32","url":null,"abstract":"The article provides an example of successful treatment of a patient with basal cell skin cancer of the lower eyelid of the right eye, stage IB cT2aN0M0, with the presence of concomitant pathology – a decrease in the activity of plasma factor XII of blood coagulation (Hageman disease). The patient’s medical history is associated with long-term ineffective treatment of the tumor with Curaderm ointment. After diagnosis and further examination at the Moscow Research Institute named after. P.A. Herzen, at the Center for Laser and Photodynamic Diagnostics and Therapy of Tumors, the patient underwent organ-preserving treatment using photodynamic therapy. 1 course of photodynamic therapy with the photosensitizer chlorin e6 was carried out. Complete tumor regression was obtained; relapse-free follow-up period was 1 year","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"8 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140260304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modern diagnostic technologies in oncodermatology","authors":"E. Filonenko, A. D. Kaprin","doi":"10.24931/2413-9432-2023-12-4-4-14","DOIUrl":"https://doi.org/10.24931/2413-9432-2023-12-4-4-14","url":null,"abstract":"Skin tumors occupy the first place in terms of incidence in the structure of oncological neoplasms. The WHO estimates that 60,000 people die each year from malignant neoplasms of the skin: 48,000 from melanoma and 12,000 from skin cancer. Timely diagnosis of skin cancer makes it possible to achieve a cure for cancer patients with long periods of relapse-free follow-up after the completion of specialized treatment. The introduction of high-tech optical methods for diagnosing skin neoplasms into clinical practice has significantly increased the specificity, sensitivity, and accuracy of diagnostics. The review is devoted to a discussion of such methods for diagnosing skin neoplasms as fluorescent diagnostics, digital dermatoscopy, SIA-scopy, and confocal microscopy. The features of the application of each of the methods are discussed, the results of the most significant Russian and foreign studies in this field are presented, as well as our own results of the practical application of a number of high-tech optical diagnostic methods at the P.A. Herzen Moscow Oncology Research Center","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"13 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140259951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatomical-functional state of surface lymphatic system of lower extremities in chronic vein diseases according to fluorescence lymphograph","authors":"Kh. A. Abduvosidov, S. M. Chudnykh, V. G. Shestakova, A. G. Alekseev, M. M. Kokoev, N. S. Kozlov, G. M. Korolyuk","doi":"10.24931/2413-9432-2023-12-4-15-21","DOIUrl":"https://doi.org/10.24931/2413-9432-2023-12-4-15-21","url":null,"abstract":"Despite the large arsenal of diagnostic methods for studying the lymphatic system, there are isolated works on its morpho-functional state in chronic venous insufluciency. The purpose of the study was to study the anatomical and physiological state of the surface lymphatic system of the lower extremities in persons with different clinical classes of chronic vein diseases using fluorescence lifography. The study was conducted in 105 patients divided into six groups according to the clinical class of chronic diseases of the veins of the lower extremities according to the CEAP classiffication. We used fluorescent lymphography using sodium fluorescein to study the anatomical and functional capabilities of the lymphotone. The study revealed that morphofunctional changes in superficial lymphatic vessels in chronic lower extremity vein diseases depend on venous system decompensation. With an increase in the clinical class of chronic diseases of the veins of the lower extremities, the rate of lymph flow through the superficial lymphatic vessels is statistically significantly reduced. At the same time, the antegrade lymph cell is completely absent in С5-С6, with the appearance of retrograde outflow and discharge of the lymph into the deep lymph vessels. Thus, the progression of chronic venous insufficiency leads to proportional progression of morphofunctional changes in the superficial lymphatic system, which leads to the formation of lymphovenous insufficiency.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"18 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140261078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photodynamic therapy of Bowen’s disease","authors":"E. Filonenko, V. Ivanova-Radkevich","doi":"10.24931/2413-9432-2023-12-4-22-29","DOIUrl":"https://doi.org/10.24931/2413-9432-2023-12-4-22-29","url":null,"abstract":"Bowen’s disease is a form of non-invasive (in situ) squamous cell skin cancer localized in the non-genital area. Russian and European clinical guidelines include photodynamic therapy (PDT) in the standard of care for patients with Bowen’s disease. In the present review, the efficacy and safety profile of different PDT regimens for Bowen’s disease are analyzed according to the available literature data. PDT can be used to treat large Bowen’s disease lesions localized in areas of the body characterized by severe healing and in cases where surgery is not feasible. Analysis of the results of studies shows that PDT is superior in efficacy and cosmetic results to traditional local treatments such as 5-fluorouracil or cryotherapy. In all analyzed studies in patients with Bowen’s disease, PDT achieved a significant clinical effect. Complete regression of pathologic foci was achieved in 67-100% of patients. In studies evaluating the recurrence rate of Bowen’s disease after PDT, this value ranged from 2-28% with the range of 6-18% in most of the studies. Most often, 5-aminolevulinic acid and its methyl ester are used for PDT in Bowen’s disease. According to some researchers, aminolevulinic acid allows to achieve a more long-term positive clinical effect with a lower incidence of painful reactions during treatment","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"13 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140259956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical case of successful application of photodynamic therapy in advanced vulvar cancer","authors":"N. A. Shanazarov, T. G. Grishachevа, G. S. Aldabergen, S. B. Smailova, Zh. Zh. Salmaganbetova","doi":"10.24931/2413-9432-2023-12-3-21-24","DOIUrl":"https://doi.org/10.24931/2413-9432-2023-12-3-21-24","url":null,"abstract":"A significant therapeutic effect of photodynamic therapy (PDT) is shown in a patient with extensive vulvar cancer after ineffective surgical and chemoradiotherapy. During the year, three courses of local PDT with a photosensitizer based on chlorin e6 were carried out. The photosensitizer was administered intravenously three hours before irradiation at a dose of 1.2 mg/kg. For laser irradiation (662 nm) of the vulvar tumor, a light guide for external irradiation was used: the power density was 0.2 W/cm 2 , the light dose was from 100 to 250 J/cm 2 . As a result of treatment, tumor regression and stable remission are observed. The patient remains under observation.","PeriodicalId":37713,"journal":{"name":"Biomedical Photonics","volume":"50 7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135414384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}