Journal of Circulating Biomarkers最新文献_第6页

The signature of circulating microparticles in heart failure patients with metabolic syndrome. 心力衰竭合并代谢综合征患者循环微粒的特征。

Journal of Circulating Biomarkers Pub Date : 2016-11-10 eCollection Date: 2016-01-01 DOI: 10.1177/1849454416663659

Alexander E Berezin, Alexander Kremzer, Tatyana Berezina, Yu Martovitskaya

{"title":"The signature of circulating microparticles in heart failure patients with metabolic syndrome.","authors":"Alexander E Berezin, Alexander Kremzer, Tatyana Berezina, Yu Martovitskaya","doi":"10.1177/1849454416663659","DOIUrl":"https://doi.org/10.1177/1849454416663659","url":null,"abstract":"The role of pattern of circulating endothelial cell-derived microparticles, platelet-derived microparticles (PMPs), and monocyte-derived microparticles (MMPs) in metabolic syndrome (MetS) patients with chronic heart failure (CHF) is not still understood. The aim of the study was to investigate a pattern of circulating microparticles (MPs) in MetS patients with CHF in relation to neurohumoral and inflammatory activation. The study retrospectively involved 101 patients with MetS and 35 healthy volunteers. Biomarkers were measured at baseline of the study. The results of the study have shown that numerous circulating PMPs- and MMPs in subjects with MetS (with or without CHF) insufficiently distinguished from level obtained in healthy volunteers. We found elevated level of CD31+/annexin V+ MPs in association with lower level of CD62E+ MPs. Therefore, we found that biomarkers of biomechanical stress serum N-terminal brain natriuretic peptide and inflammation (high-sensitive C-reactive protein ,osteoprotegerin) remain statistically significant predictors for decreased CD62E+ to CD31+/annexin V+ ratio in MetS patients with CHF. In conclusion, decreased CD62E+ to CD31+/annexin V+ ratio reflected that impaired immune phenotype of MPs may be discussed as a surrogate marker of CHF development in MetS population.","PeriodicalId":37524,"journal":{"name":"Journal of Circulating Biomarkers","volume":"5 ","pages":"1849454416663659"},"PeriodicalIF":0.0,"publicationDate":"2016-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1849454416663659","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35427688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Microvesicles shed from fibroblasts act as metalloproteinase carriers in a 3-D collagen matrix. 从成纤维细胞脱落的微泡在三维胶原基质中充当金属蛋白酶的载体。

Journal of Circulating Biomarkers Pub Date : 2016-11-07 eCollection Date: 2016-01-01 DOI: 10.1177/1849454416663660

Valentina Laghezza Masci, Anna Rita Taddei, Gabriella Gambellini, Franco Giorgi, Anna Maria Fausto

{"title":"Microvesicles shed from fibroblasts act as metalloproteinase carriers in a 3-D collagen matrix.","authors":"Valentina Laghezza Masci, Anna Rita Taddei, Gabriella Gambellini, Franco Giorgi, Anna Maria Fausto","doi":"10.1177/1849454416663660","DOIUrl":"https://doi.org/10.1177/1849454416663660","url":null,"abstract":"This study shows that fibroblasts migrating into a collagen matrix release numerous microvesicles into the surrounding medium. By spreading in regions of the matrix far distant from cells of origin, microvesicles carry metalloproteinase 9 (MMP-9) to act upon the collagen fibrils. As a result, the collagen matrix is gradually transformed from a laminar to a fibrillar type of architecture. As shown by western blots and gelatin zymography, MMP-9 is secreted as a 92 kDa precursor and activated upon release of 82 kDa product into the culture medium. Activation is more efficient under three-dimensional than in two-dimensional culturing conditions. While MMP-9 labeling is associated with intraluminal vesicles clustered inside the microvesicles, the microvesicle's integrin β1 marker is bound to the outer membrane. The intraluminal vesicles are recruited from the cortical cytoplasm and eventually released following uploading inside the microvesicle. Here, we propose that fusion of the intraluminal vesicles with the outer microvesicle's membrane could work as a mechanism controlling the extent to which MMP-9 is first activated and then released extracellularly.","PeriodicalId":37524,"journal":{"name":"Journal of Circulating Biomarkers","volume":"5 ","pages":"1849454416663660"},"PeriodicalIF":0.0,"publicationDate":"2016-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1849454416663660","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35427689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Novel blood test to predict neoplastic activity in healthy patients and metastatic recurrence after primary tumor resection. 预测健康患者肿瘤活动性和原发肿瘤切除后转移性复发的新型血液检测。

Journal of Circulating Biomarkers Pub Date : 2016-11-04 eCollection Date: 2016-01-01 DOI: 10.1177/1849454416663661

Mohamed Abdouh, Dana Hamam, Vincenzo Arena, Manuel Arena, Hussam Alamri, Goffredo Orazio Arena

{"title":"Novel blood test to predict neoplastic activity in healthy patients and metastatic recurrence after primary tumor resection.","authors":"Mohamed Abdouh, Dana Hamam, Vincenzo Arena, Manuel Arena, Hussam Alamri, Goffredo Orazio Arena","doi":"10.1177/1849454416663661","DOIUrl":"https://doi.org/10.1177/1849454416663661","url":null,"abstract":"We reported that single oncosuppressor-mutated (SOM) cells turn malignant when exposed to cancer patients' sera. We tested the possibility to incorporate this discovery into a biological platform able to detect cancer in healthy individuals and to predict metastases after tumor resection. Blood was drawn prior to tumor resection and within a year after surgery. Blood samples from healthy individuals or metastatic patients were used as negative and positive controls, respectively. Patients at risk for cancer were included in the screening cohort. Once treated, cells were injected into nonobese diabetic/severe combined immunodeficiency mice to monitor tumor growth. All samples of sera coming from metastatic patients transformed SOM cells into malignant cells. Four samples from screened patients transformed SOM cells. Further clinical tests done on these patients showed the presence of early cancerous lesions despite normal tumor markers. Based on the xenotransplants size, we were able to predict metastasis in three patients before diagnostic tests confirmed the presence of the metastatic lesions. These data show that this serum-based platform has potentials to be used for cancer screening and for identification of patients at risks to develop metastases regardless of the Tumor Node Metastasis (TNM) stage or tumor markers level.","PeriodicalId":37524,"journal":{"name":"Journal of Circulating Biomarkers","volume":"5 ","pages":"1849454416663661"},"PeriodicalIF":0.0,"publicationDate":"2016-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1849454416663661","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35428135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

X-rays and metformin cause increased urinary excretion of cell-free nuclear and mitochondrial DNA in aged rats. x射线和二甲双胍引起老年大鼠无细胞核和线粒体DNA尿排泄增加。

Journal of Circulating Biomarkers Pub Date : 2016-10-25 eCollection Date: 2016-01-01 DOI: 10.1177/1849454416670782

Azhub Gaziev, Serazhutdin Abdullaev, Gulchachak Minkabirova, Kristina Kamenskikh

{"title":"X-rays and metformin cause increased urinary excretion of cell-free nuclear and mitochondrial DNA in aged rats.","authors":"Azhub Gaziev, Serazhutdin Abdullaev, Gulchachak Minkabirova, Kristina Kamenskikh","doi":"10.1177/1849454416670782","DOIUrl":"https://doi.org/10.1177/1849454416670782","url":null,"abstract":"Activation of cell death in mammals can be assessed by an increase of an amount of cell-free DNA (cf-DNA) in urine or plasma. We investigated the excretion of cf nuclear DNA (nDNA) and cf mitochondrial DNA (mtDNA) in the urine of rats 3 and 24 months in age after X-irradiation and metformin administration. Analyses showed that prior to treatment, the amount of cf-nDNA was 40% higher and cf-mtDNA was 50% higher in the urine of aged rats compared to that of young animals. At 12 h after irradiation, the content of cf-nDNA and cf-mtDNA in the urine of young rats was increased by 200% and 460%, respectively, relative to the control, whereas in the urine of aged rats, it was 250% and 720% higher. After 6 h following metformin administration, the amount of cf-nDNA and cf-mtDNA in the urine of young rats was elevated by 25% and 55% and by 50% and 160% in the urine of aged rats. Thus, these preliminary data suggest that X-rays and metformin cause a significant increase of cf-DNA in the urine of older rats caused by the active cell death in tissues. These results also suggest that metformin possibly initiates the death of the cells containing structural and functional abnormalities.","PeriodicalId":37524,"journal":{"name":"Journal of Circulating Biomarkers","volume":"5 ","pages":"1849454416670782"},"PeriodicalIF":0.0,"publicationDate":"2016-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1849454416670782","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35428136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Addition of thrombin reduces the recovery of extracellular vesicles from blood plasma. 凝血酶的加入减少了血浆中细胞外囊泡的恢复。

Journal of Circulating Biomarkers Pub Date : 2016-10-04 eCollection Date: 2016-01-01 DOI: 10.1177/1849454416663648

Anush Arakelyan, Wendy Fitzgerald, Murad Vagida, Elena Vasilieva, Leonid Margolis, Jean-Charles Grivel

引用次数: 6

Circulating cell-free DNA is a predictor of short-term neurological outcome in stroke patients treated with intravenous thrombolysis. 循环无细胞DNA是静脉溶栓治疗的脑卒中患者短期神经预后的预测因子。

Journal of Circulating Biomarkers Pub Date : 2016-09-26 eCollection Date: 2016-01-01 DOI: 10.1177/1849454416668791

Alejandro Bustamante, Fernando Mancha, Hada C Macher, Teresa García-Berrocoso, Dolors Giralt, Marc Ribó, Juan M Guerrero, Joan Montaner

{"title":"Circulating cell-free DNA is a predictor of short-term neurological outcome in stroke patients treated with intravenous thrombolysis.","authors":"Alejandro Bustamante, Fernando Mancha, Hada C Macher, Teresa García-Berrocoso, Dolors Giralt, Marc Ribó, Juan M Guerrero, Joan Montaner","doi":"10.1177/1849454416668791","DOIUrl":"https://doi.org/10.1177/1849454416668791","url":null,"abstract":"Circulating cell-free DNA (cfDNA) has been described as a prognostic marker for several diseases. Its prognostic value for short-term outcome in stroke patients treated with intravenous thrombolysis remains unexplored. cfDNA was measured on admission in 54 tissue plasminogen activator (tPA)-treated patients and 15 healthy controls using a real-time quantitative polymerase chain reaction assay. Neurological outcome was assessed at 48 h. Predictors of neurological improvement were evaluated by logistic regression analysis, and the additional predictive value of cfDNA over clinical variables was determined by integrated discrimination improvement (IDI). Stroke patients presented higher baseline cfDNA than healthy controls (408.5 (179-700.5) vs. 153.5 (66.9-700.5) kilogenome-equivalents/L, p = 0.123). A trend towards lower cfDNA levels was found in patients who neurologically improved at 48 h (269.5 (143.3-680) vs. 504 (345.9-792.3) kilogenome-equivalents/L, p = 0.130). In logistic regression analysis, recanalization at 1 h and cfDNA < 302.75 kilogenome-equivalents/L was independently associated with neurological improvement after adjustment by age, gender and baseline National Institutes of Health Stroke Scale score. The addition of cfDNA to the clinical predictive model improved its discrimination (IDI = 21.2% (9.2-33.3%), p = 0.009). These data suggest that cfDNA could be a surrogate marker for monitoring tPA efficacy by the prediction of short-term neurological outcome.","PeriodicalId":37524,"journal":{"name":"Journal of Circulating Biomarkers","volume":"5 ","pages":"1849454416668791"},"PeriodicalIF":0.0,"publicationDate":"2016-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1849454416668791","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35427690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Elevated Adiponectin Antibody Levels in Sera of Patients with Atherosclerosis-Related Coronary Artery Disease, Cerebral Infarction and Diabetes Mellitus. 动脉粥样硬化相关性冠心病、脑梗死和糖尿病患者血清脂联素抗体水平升高

Journal of Circulating Biomarkers Pub Date : 2016-04-07 eCollection Date: 2016-01-01 DOI: 10.5772/63218

Takaki Hiwasa, Xiao-Meng Zhang, Risa Kimura, Mikiko Ohno, Po-Min Chen, Eiichiro Nishi, Koh Ono, Takeshi Kimura, Ikuo Kamitsukasa, Takeshi Wada, Akiyo Aotsuka, Seiichiro Mine, Hirotaka Takizawa, Koichi Kashiwado, Minoru Takemoto, Kazuki Kobayashi, Harukiyo Kawamura, Ryoichi Ishibashi, Koutaro Yokote, Rika Nakamura, Go Tomiyoshi, Natsuko Shinmen, Hideyuki Kuroda

{"title":"Elevated Adiponectin Antibody Levels in Sera of Patients with Atherosclerosis-Related Coronary Artery Disease, Cerebral Infarction and Diabetes Mellitus.","authors":"Takaki Hiwasa, Xiao-Meng Zhang, Risa Kimura, Mikiko Ohno, Po-Min Chen, Eiichiro Nishi, Koh Ono, Takeshi Kimura, Ikuo Kamitsukasa, Takeshi Wada, Akiyo Aotsuka, Seiichiro Mine, Hirotaka Takizawa, Koichi Kashiwado, Minoru Takemoto, Kazuki Kobayashi, Harukiyo Kawamura, Ryoichi Ishibashi, Koutaro Yokote, Rika Nakamura, Go Tomiyoshi, Natsuko Shinmen, Hideyuki Kuroda","doi":"10.5772/63218","DOIUrl":"https://doi.org/10.5772/63218","url":null,"abstract":"Adiponectin secreted from the adipocytes plays pleiotropic, anti-atherosclerotic roles, such as enhancement of insulin secretion and an increase in energy expenditure. The measurement of levels of circulating adiponectin is useful to evaluate the progression of atherosclerosis-related diseases, such as coronary artery disease (CAD), cerebral infarction (CI) and diabetes mellitus (DM). We examined the serum antibody levels against recombinant adiponectin protein via the amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) method. The results revealed that the antibody levels were significantly higher in patients with CAD, CI and type 2 DM, than in healthy donors. Receiver operating curve analysis showed that the sensitivity was in a range of 41-48% for CAD, CI and DM. Thus, the serum anti-adiponectin antibody levels could be a common marker for atherosclerosis-related diseases.","PeriodicalId":37524,"journal":{"name":"Journal of Circulating Biomarkers","volume":"5 ","pages":"8"},"PeriodicalIF":0.0,"publicationDate":"2016-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/63218","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35535116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Exosome Secretion - More Than Simple Waste Disposal? Implications for Physiology, Diagnostics and Therapeutics. 外泌体分泌-不仅仅是简单的废物处理?对生理学、诊断学和治疗学的启示。

Journal of Circulating Biomarkers Pub Date : 2016-04-01 eCollection Date: 2016-01-01 DOI: 10.5772/62975

Sivappriyan Nagarajah

引用次数: 24

Prognostication in Different Heart Failure Phenotypes: The Role of Circulating Biomarkers. 不同心衰表型的预后:循环生物标志物的作用。

Journal of Circulating Biomarkers Pub Date : 2016-03-16 eCollection Date: 2016-01-01 DOI: 10.5772/62797

Alexander E Berezin

{"title":"Prognostication in Different Heart Failure Phenotypes: The Role of Circulating Biomarkers.","authors":"Alexander E Berezin","doi":"10.5772/62797","DOIUrl":"https://doi.org/10.5772/62797","url":null,"abstract":"Heart failure (HF) is multifactorial syndrome with high cardiovascular (CV) morbidity and mortality rates associated with an increasing prevalence worldwide. Measuring plasma levels of circulating biomarkers, i.e., natriuretic peptides, cardiac-specific troponins, metabolomic intermediates, Galectin-3, ST2, cardiotrophin-1, soluble endoglin and growth differentiation factor 15, may assist in the prognostication of HF development. However, the role of biomarker models in the prediction of an early stage of HF with a preserved ejection fraction (HFpEF) and HF with a reduced ejection fraction (HFrEF) is not still understood. This review explores the knowledge regarding the utility of cardiac biomarkers, aiming to reclassify patients with different phenotypes of HF. The review reports that several biomarkers reflected on subsequently alter collagen turnover, cardiac fibrosis and inflammation, which might have diagnostic and predictive value in HFpEF and HFrEF. The best candidates for determining the early stage of HF development were sST2, Galectin-3, CT-1 and GDF-15. However, increased plasma concentrations of these biomarkers were not specific to a distinct disease group of HFpEF and HFrEF. Finally, more investigations are required to determine the role of novel biomarkers in the prediction of HF and the determination of the early stages of HFpEF and HFrEF development.","PeriodicalId":37524,"journal":{"name":"Journal of Circulating Biomarkers","volume":"5 ","pages":"6"},"PeriodicalIF":0.0,"publicationDate":"2016-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/62797","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35535114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 53

Analyses of 123 Peripheral Human Immune Cell Subsets: Defining Differences with Age and between Healthy Donors and Cancer Patients Not Detected in Analysis of Standard Immune Cell Types. 123个人类外周免疫细胞亚群的分析:在标准免疫细胞类型分析中未检测到的年龄和健康供体与癌症患者之间的差异

Journal of Circulating Biomarkers Pub Date : 2016-03-10 eCollection Date: 2016-01-01 DOI: 10.5772/62322

Lauren M Lepone, Renee N Donahue, Italia Grenga, Simon Metenou, Jacob Richards, Christopher R Heery, Ravi A Madan, James L Gulley, Jeffrey Schlom

{"title":"Analyses of 123 Peripheral Human Immune Cell Subsets: Defining Differences with Age and between Healthy Donors and Cancer Patients Not Detected in Analysis of Standard Immune Cell Types.","authors":"Lauren M Lepone, Renee N Donahue, Italia Grenga, Simon Metenou, Jacob Richards, Christopher R Heery, Ravi A Madan, James L Gulley, Jeffrey Schlom","doi":"10.5772/62322","DOIUrl":"https://doi.org/10.5772/62322","url":null,"abstract":"Recent advances in human immunology have led to the identification of novel immune cell subsets and the biological function of many of these subsets has now been identified. The recent US Food and Drug Administration approval of several immunotherapeutics for the treatment of a variety of cancer types and the results of ongoing immunotherapy clinical studies requires a more thorough interrogation of the immune system. We report here the use of flow cytometry-based analyses to identify 123 immune cell subsets of peripheral blood mononuclear cells. The use of these panels defines multiple differences in younger (< 40 years) vs. older (≥ 40 years) individuals and between aged-matched apparently healthy individuals and metastatic cancer patients, aspects not seen in the analysis of the following standard immune cell types: CD8, CD4, natural killer, natural killer-T, regulatory T, myeloid derived suppressor cells, conventional dendritic cells (DCs), plasmacytoid DCs and B cells. The use of these panels identifying 123 immune cell subsets may aid in the identification of patients who may benefit from immunotherapy, either prior to therapy or early in the immunotherapeutic regimen, for the treatment of cancer or other chronic or infectious diseases.","PeriodicalId":37524,"journal":{"name":"Journal of Circulating Biomarkers","volume":"5 ","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2016-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/62322","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35535113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49