Journal of Circulating Biomarkers最新文献_第7页

X-rays and metformin cause increased urinary excretion of cell-free nuclear and mitochondrial DNA in aged rats. x射线和二甲双胍引起老年大鼠无细胞核和线粒体DNA尿排泄增加。

Journal of Circulating Biomarkers Pub Date : 2016-10-25 eCollection Date: 2016-01-01 DOI: 10.1177/1849454416670782

Azhub Gaziev, Serazhutdin Abdullaev, Gulchachak Minkabirova, Kristina Kamenskikh

{"title":"X-rays and metformin cause increased urinary excretion of cell-free nuclear and mitochondrial DNA in aged rats.","authors":"Azhub Gaziev, Serazhutdin Abdullaev, Gulchachak Minkabirova, Kristina Kamenskikh","doi":"10.1177/1849454416670782","DOIUrl":"https://doi.org/10.1177/1849454416670782","url":null,"abstract":"Activation of cell death in mammals can be assessed by an increase of an amount of cell-free DNA (cf-DNA) in urine or plasma. We investigated the excretion of cf nuclear DNA (nDNA) and cf mitochondrial DNA (mtDNA) in the urine of rats 3 and 24 months in age after X-irradiation and metformin administration. Analyses showed that prior to treatment, the amount of cf-nDNA was 40% higher and cf-mtDNA was 50% higher in the urine of aged rats compared to that of young animals. At 12 h after irradiation, the content of cf-nDNA and cf-mtDNA in the urine of young rats was increased by 200% and 460%, respectively, relative to the control, whereas in the urine of aged rats, it was 250% and 720% higher. After 6 h following metformin administration, the amount of cf-nDNA and cf-mtDNA in the urine of young rats was elevated by 25% and 55% and by 50% and 160% in the urine of aged rats. Thus, these preliminary data suggest that X-rays and metformin cause a significant increase of cf-DNA in the urine of older rats caused by the active cell death in tissues. These results also suggest that metformin possibly initiates the death of the cells containing structural and functional abnormalities.","PeriodicalId":37524,"journal":{"name":"Journal of Circulating Biomarkers","volume":"5 ","pages":"1849454416670782"},"PeriodicalIF":0.0,"publicationDate":"2016-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1849454416670782","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35428136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Addition of thrombin reduces the recovery of extracellular vesicles from blood plasma. 凝血酶的加入减少了血浆中细胞外囊泡的恢复。

Journal of Circulating Biomarkers Pub Date : 2016-10-04 eCollection Date: 2016-01-01 DOI: 10.1177/1849454416663648

Anush Arakelyan, Wendy Fitzgerald, Murad Vagida, Elena Vasilieva, Leonid Margolis, Jean-Charles Grivel

引用次数: 6

Circulating cell-free DNA is a predictor of short-term neurological outcome in stroke patients treated with intravenous thrombolysis. 循环无细胞DNA是静脉溶栓治疗的脑卒中患者短期神经预后的预测因子。

Journal of Circulating Biomarkers Pub Date : 2016-09-26 eCollection Date: 2016-01-01 DOI: 10.1177/1849454416668791

Alejandro Bustamante, Fernando Mancha, Hada C Macher, Teresa García-Berrocoso, Dolors Giralt, Marc Ribó, Juan M Guerrero, Joan Montaner

{"title":"Circulating cell-free DNA is a predictor of short-term neurological outcome in stroke patients treated with intravenous thrombolysis.","authors":"Alejandro Bustamante, Fernando Mancha, Hada C Macher, Teresa García-Berrocoso, Dolors Giralt, Marc Ribó, Juan M Guerrero, Joan Montaner","doi":"10.1177/1849454416668791","DOIUrl":"https://doi.org/10.1177/1849454416668791","url":null,"abstract":"Circulating cell-free DNA (cfDNA) has been described as a prognostic marker for several diseases. Its prognostic value for short-term outcome in stroke patients treated with intravenous thrombolysis remains unexplored. cfDNA was measured on admission in 54 tissue plasminogen activator (tPA)-treated patients and 15 healthy controls using a real-time quantitative polymerase chain reaction assay. Neurological outcome was assessed at 48 h. Predictors of neurological improvement were evaluated by logistic regression analysis, and the additional predictive value of cfDNA over clinical variables was determined by integrated discrimination improvement (IDI). Stroke patients presented higher baseline cfDNA than healthy controls (408.5 (179-700.5) vs. 153.5 (66.9-700.5) kilogenome-equivalents/L, p = 0.123). A trend towards lower cfDNA levels was found in patients who neurologically improved at 48 h (269.5 (143.3-680) vs. 504 (345.9-792.3) kilogenome-equivalents/L, p = 0.130). In logistic regression analysis, recanalization at 1 h and cfDNA < 302.75 kilogenome-equivalents/L was independently associated with neurological improvement after adjustment by age, gender and baseline National Institutes of Health Stroke Scale score. The addition of cfDNA to the clinical predictive model improved its discrimination (IDI = 21.2% (9.2-33.3%), p = 0.009). These data suggest that cfDNA could be a surrogate marker for monitoring tPA efficacy by the prediction of short-term neurological outcome.","PeriodicalId":37524,"journal":{"name":"Journal of Circulating Biomarkers","volume":"5 ","pages":"1849454416668791"},"PeriodicalIF":0.0,"publicationDate":"2016-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/1849454416668791","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35427690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Elevated Adiponectin Antibody Levels in Sera of Patients with Atherosclerosis-Related Coronary Artery Disease, Cerebral Infarction and Diabetes Mellitus. 动脉粥样硬化相关性冠心病、脑梗死和糖尿病患者血清脂联素抗体水平升高

Journal of Circulating Biomarkers Pub Date : 2016-04-07 eCollection Date: 2016-01-01 DOI: 10.5772/63218

Takaki Hiwasa, Xiao-Meng Zhang, Risa Kimura, Mikiko Ohno, Po-Min Chen, Eiichiro Nishi, Koh Ono, Takeshi Kimura, Ikuo Kamitsukasa, Takeshi Wada, Akiyo Aotsuka, Seiichiro Mine, Hirotaka Takizawa, Koichi Kashiwado, Minoru Takemoto, Kazuki Kobayashi, Harukiyo Kawamura, Ryoichi Ishibashi, Koutaro Yokote, Rika Nakamura, Go Tomiyoshi, Natsuko Shinmen, Hideyuki Kuroda

{"title":"Elevated Adiponectin Antibody Levels in Sera of Patients with Atherosclerosis-Related Coronary Artery Disease, Cerebral Infarction and Diabetes Mellitus.","authors":"Takaki Hiwasa, Xiao-Meng Zhang, Risa Kimura, Mikiko Ohno, Po-Min Chen, Eiichiro Nishi, Koh Ono, Takeshi Kimura, Ikuo Kamitsukasa, Takeshi Wada, Akiyo Aotsuka, Seiichiro Mine, Hirotaka Takizawa, Koichi Kashiwado, Minoru Takemoto, Kazuki Kobayashi, Harukiyo Kawamura, Ryoichi Ishibashi, Koutaro Yokote, Rika Nakamura, Go Tomiyoshi, Natsuko Shinmen, Hideyuki Kuroda","doi":"10.5772/63218","DOIUrl":"https://doi.org/10.5772/63218","url":null,"abstract":"Adiponectin secreted from the adipocytes plays pleiotropic, anti-atherosclerotic roles, such as enhancement of insulin secretion and an increase in energy expenditure. The measurement of levels of circulating adiponectin is useful to evaluate the progression of atherosclerosis-related diseases, such as coronary artery disease (CAD), cerebral infarction (CI) and diabetes mellitus (DM). We examined the serum antibody levels against recombinant adiponectin protein via the amplified luminescent proximity homogeneous assay-linked immunosorbent assay (AlphaLISA) method. The results revealed that the antibody levels were significantly higher in patients with CAD, CI and type 2 DM, than in healthy donors. Receiver operating curve analysis showed that the sensitivity was in a range of 41-48% for CAD, CI and DM. Thus, the serum anti-adiponectin antibody levels could be a common marker for atherosclerosis-related diseases.","PeriodicalId":37524,"journal":{"name":"Journal of Circulating Biomarkers","volume":"5 ","pages":"8"},"PeriodicalIF":0.0,"publicationDate":"2016-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/63218","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35535116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Exosome Secretion - More Than Simple Waste Disposal? Implications for Physiology, Diagnostics and Therapeutics. 外泌体分泌-不仅仅是简单的废物处理?对生理学、诊断学和治疗学的启示。

Journal of Circulating Biomarkers Pub Date : 2016-04-01 eCollection Date: 2016-01-01 DOI: 10.5772/62975

Sivappriyan Nagarajah

引用次数: 24

Prognostication in Different Heart Failure Phenotypes: The Role of Circulating Biomarkers. 不同心衰表型的预后:循环生物标志物的作用。

Journal of Circulating Biomarkers Pub Date : 2016-03-16 eCollection Date: 2016-01-01 DOI: 10.5772/62797

Alexander E Berezin

{"title":"Prognostication in Different Heart Failure Phenotypes: The Role of Circulating Biomarkers.","authors":"Alexander E Berezin","doi":"10.5772/62797","DOIUrl":"https://doi.org/10.5772/62797","url":null,"abstract":"Heart failure (HF) is multifactorial syndrome with high cardiovascular (CV) morbidity and mortality rates associated with an increasing prevalence worldwide. Measuring plasma levels of circulating biomarkers, i.e., natriuretic peptides, cardiac-specific troponins, metabolomic intermediates, Galectin-3, ST2, cardiotrophin-1, soluble endoglin and growth differentiation factor 15, may assist in the prognostication of HF development. However, the role of biomarker models in the prediction of an early stage of HF with a preserved ejection fraction (HFpEF) and HF with a reduced ejection fraction (HFrEF) is not still understood. This review explores the knowledge regarding the utility of cardiac biomarkers, aiming to reclassify patients with different phenotypes of HF. The review reports that several biomarkers reflected on subsequently alter collagen turnover, cardiac fibrosis and inflammation, which might have diagnostic and predictive value in HFpEF and HFrEF. The best candidates for determining the early stage of HF development were sST2, Galectin-3, CT-1 and GDF-15. However, increased plasma concentrations of these biomarkers were not specific to a distinct disease group of HFpEF and HFrEF. Finally, more investigations are required to determine the role of novel biomarkers in the prediction of HF and the determination of the early stages of HFpEF and HFrEF development.","PeriodicalId":37524,"journal":{"name":"Journal of Circulating Biomarkers","volume":"5 ","pages":"6"},"PeriodicalIF":0.0,"publicationDate":"2016-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/62797","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35535114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 53

Analyses of 123 Peripheral Human Immune Cell Subsets: Defining Differences with Age and between Healthy Donors and Cancer Patients Not Detected in Analysis of Standard Immune Cell Types. 123个人类外周免疫细胞亚群的分析:在标准免疫细胞类型分析中未检测到的年龄和健康供体与癌症患者之间的差异

Journal of Circulating Biomarkers Pub Date : 2016-03-10 eCollection Date: 2016-01-01 DOI: 10.5772/62322

Lauren M Lepone, Renee N Donahue, Italia Grenga, Simon Metenou, Jacob Richards, Christopher R Heery, Ravi A Madan, James L Gulley, Jeffrey Schlom

{"title":"Analyses of 123 Peripheral Human Immune Cell Subsets: Defining Differences with Age and between Healthy Donors and Cancer Patients Not Detected in Analysis of Standard Immune Cell Types.","authors":"Lauren M Lepone, Renee N Donahue, Italia Grenga, Simon Metenou, Jacob Richards, Christopher R Heery, Ravi A Madan, James L Gulley, Jeffrey Schlom","doi":"10.5772/62322","DOIUrl":"https://doi.org/10.5772/62322","url":null,"abstract":"Recent advances in human immunology have led to the identification of novel immune cell subsets and the biological function of many of these subsets has now been identified. The recent US Food and Drug Administration approval of several immunotherapeutics for the treatment of a variety of cancer types and the results of ongoing immunotherapy clinical studies requires a more thorough interrogation of the immune system. We report here the use of flow cytometry-based analyses to identify 123 immune cell subsets of peripheral blood mononuclear cells. The use of these panels defines multiple differences in younger (< 40 years) vs. older (≥ 40 years) individuals and between aged-matched apparently healthy individuals and metastatic cancer patients, aspects not seen in the analysis of the following standard immune cell types: CD8, CD4, natural killer, natural killer-T, regulatory T, myeloid derived suppressor cells, conventional dendritic cells (DCs), plasmacytoid DCs and B cells. The use of these panels identifying 123 immune cell subsets may aid in the identification of patients who may benefit from immunotherapy, either prior to therapy or early in the immunotherapeutic regimen, for the treatment of cancer or other chronic or infectious diseases.","PeriodicalId":37524,"journal":{"name":"Journal of Circulating Biomarkers","volume":"5 ","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2016-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/62322","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35535113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49

Protein Complexes in Urine Interfere with Extracellular Vesicle Biomarker Studies. 尿中的蛋白质复合物干扰细胞外囊泡生物标志物研究。

Journal of Circulating Biomarkers Pub Date : 2016-03-01 eCollection Date: 2016-01-01 DOI: 10.5772/62579

Magda Wachalska, Danijela Koppers-Lalic, Monique van Eijndhoven, Michiel Pegtel, Albert A Geldof, Andrea D Lipinska, R Jeroen van Moorselaar, Irene V Bijnsdorp

{"title":"Protein Complexes in Urine Interfere with Extracellular Vesicle Biomarker Studies.","authors":"Magda Wachalska, Danijela Koppers-Lalic, Monique van Eijndhoven, Michiel Pegtel, Albert A Geldof, Andrea D Lipinska, R Jeroen van Moorselaar, Irene V Bijnsdorp","doi":"10.5772/62579","DOIUrl":"https://doi.org/10.5772/62579","url":null,"abstract":"Urine exosomes (extracellular vesicles; EVs) contain (micro)RNA (miRNA) and protein biomarkers that are useful for the non-invasive diagnosis of various urological diseases. However, the urinary Tamm-Horsfall protein (THP) complex, which forms at reduced temperatures, may affect EV isolation and may also lead to contamination by other molecules including microRNAs (miRNAs). Therefore, we compared the levels of three miRNAs within the purified EV fraction and THP- protein-network. Urine was collected from healthy donors and EVs were isolated by ultracentrifugation (UC), two commercial kits or sepharose size-exclusion chromatography (SEC). SEC enables the separation of EVs from protein-complexes in urine. After UC, the isolation of EV-miRNA was compared with two commercial kits. The EV isolation efficiency was evaluated by measuring the EV protein markers, Alix and TSG101, CD63 by Western blotting, or miR-375, miR-204 and miR-21 by RT-qPCR. By using commercial kits, EV isolation resulted in either low yields or dissimilar miRNA levels. Via SEC, the EVs were separated from the protein-complex fraction. Importantly, a different ratio was observed between the three miRNAs in the protein fraction compared to the EV fraction. Thus, protein-complexes within urine may influence EV-biomarker studies. Therefore, the characterization of the isolated EV fraction is important to obtain reproducible results.","PeriodicalId":37524,"journal":{"name":"Journal of Circulating Biomarkers","volume":"5 ","pages":"4"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/62579","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35535112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication. 研究非接触细胞外囊泡通讯的细胞培养系统的特性。

Journal of Circulating Biomarkers Pub Date : 2016-02-26 eCollection Date: 2016-01-01 DOI: 10.5772/62580

Anne Louise Schacht Revenfeld, Evo Kristina Lindersson Søndergaard, Allan Stensballe, Rikke Bæk, Malene Møller Jørgensen, Kim Varming

{"title":"Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication.","authors":"Anne Louise Schacht Revenfeld, Evo Kristina Lindersson Søndergaard, Allan Stensballe, Rikke Bæk, Malene Møller Jørgensen, Kim Varming","doi":"10.5772/62580","DOIUrl":"https://doi.org/10.5772/62580","url":null,"abstract":"Appropriate and well-documented in vitro cell-culturing systems are necessary to study the activity and biological function of extracellular vesicles (EVs). The aim of this study was to describe an experimental system, in which dynamic, vesicle-based cell communication can be investigated. A commercially available cell-culturing system was applied to study contact-independent cell communication, which separated two cell populations using a membrane with a pore size of 0.4 μm. The EV exchange characteristics between the two compartments in the culture set-up was preliminarily investigated in a cell-free set-up, and analysed using the Extracellular Vesicle (EV) Array and Nanoparticle Tracking Analysis. The application of the cell-culturing set-up was demonstrated using co-cultures of human primary cells. The effects of the relative placement of the two cell populations on the phenotype of EVs found in the cell supernatant were investigated. The results indicate that this placement can be important for the biological hypothesis that is being investigated. These observations are relevant for short (<24h) as well as long (several days) studies of vesicle-based cell communication. Moreover, the introduced cell-culturing set-up and analytical strategy can be used to study contact-independent vesicle communication in a reproducible manner.","PeriodicalId":37524,"journal":{"name":"Journal of Circulating Biomarkers","volume":"5 ","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2016-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/62580","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35535111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Onward to 2016. 进入2016年。

Journal of Circulating Biomarkers Pub Date : 2016-01-28 eCollection Date: 2016-01-01 DOI: 10.5772/62278

Shidong Jia, Antonio Chiesi, Winston Patrick Kuo

引用次数: 0