BioImpacts : BI最新文献

{"title":"Recent advances in immunosensor for narcotic drug detection","authors":"S. Gandhi, P. Suman, Ashok Kumar, Prince Sharma, N. Capalash, Raman Suri","doi":"10.15171/bi.2015.30","DOIUrl":"https://doi.org/10.15171/bi.2015.30","url":null,"abstract":"Introduction: Immunosensor for illicit drugs have gained immense interest and have found several applications for drug abuse monitoring. This technology has offered a low cost detection of narcotics; thereby, providing a confirmatory platform to compliment the existing analytical methods. Methods: In this minireview, we define the basic concept of transducer for immunosensor development that utilizes antibodies and low molecular mass hapten (opiate) molecules. Results: This article emphasizes on recent advances in immunoanalytical techniques for monitoring of opiate drugs. Our results demonstrate that high quality antibodies can be used for immunosensor development against target analyte with greater sensitivity, specificity and precision than other available analytical methods. Conclusion: In this review we highlight the fundamentals of different transducer technologies and its applications for immunosensor development currently being developed in our laboratory using rapid screening via immunochromatographic kit, label free optical detection via enzyme, fluorescence, gold nanoparticles and carbon nanotubes based immunosensing for sensitive and specific monitoring of opiates.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115775267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laboratory environment and bio-medical experience: the impact of administration technique on the quality of immune-behavior data results in stress experience","authors":"Nessaibia Issam, T. Abdelkrim, Chouba Ibtissem, Kaarar Narjess","doi":"10.15171/bi.2015.25","DOIUrl":"https://doi.org/10.15171/bi.2015.25","url":null,"abstract":"Introduction: Often in an experiment, the control group and the intact group are not identified because most scientists neglect the fact that the sets of manipulation as technical administrations may be considered as an undesirable stress on the clarity of the data obtained from a scientific research specifically if it focuses on studying the effects of stress. Methods: This study was conducted in two parts using 40 male Wistar rats. The first part aimed to treat a group of rats by repeated injections i.p route (1 mL/kg) of placebo or NaCl (0.9%) and the other by direct oral administration of NaCl (0.9%). Both groups spent 1 h of jet air stress with stressed group. Our objective was to consider the effects that these manipulations would have on the validity of behavioral results (the elevated plus maze test, the open field, the light/dark box test) and immune data (immune cell count) during this stress experience. The second part was devoted to the measurement of ACTH, IL6, and CRP in these experimental groups. Results: Unlike oral administration, repeated intra-peritoneal injections cause a significant increase of plasma obtained levels of the adrenocorticotropin hormone (ACTH), interleukin-6 (IL-6) and the C-reactive protein (CRP) using injections of placebo: NaCl 0.9% (1 mL/kg) and it may have side effect on significant immune and behavioral alterations data quality induced by 1 h of air jet in the animal’s cage identified by the leukocyte formula and behavioral tests. Conclusion: In an experimental protocol conducted on animal models, it is essential to opt for painless techniques such as oral administration instead of painful injections to avoid confusion at the behavioral and immunological results from biomedical experiments specifically one that focuses on the stress study.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114145912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Network of nanomedicine researches: impact of Iranian scientists","authors":"M. Biglu, S. Riazi","doi":"10.15171/bi.2015.29","DOIUrl":"https://doi.org/10.15171/bi.2015.29","url":null,"abstract":"Introduction: We may define the nanomedicine as the use of nanotechnology in the health care, disease diagnoses and treatment in order to maintain and increase the health status of a population through improve pharmacotherapy. The main objective of the current study is to analyze and visualize the co-authorship network of all papers in the field of nanomedicine published throughout 2002-2014 in journals and indexed in the Web of Science database. Methods: The Web of Science database was used to extract all papers indexed as a topic of nanomedicine through 2002-2014. The Science of Science Tool was used to map the co-authorship network of papers. Results: Total number of papers extracted from the Web of Science in the field of nanomedicine was 3092 through 2002-2014. Analysis of data showed that the research activities in the field of nanomedicine increased steadily through the period of study. USA, China, and India were the most prolific countries in the field. The dominant language of publications was English. The co-authorship connection revealed a network with a density of 0.0006. Conclusion: Nanomedicine researches have markedly been increased in Iran. Ninety-five percent of Iranian papers were cooperated with multi-authors. The collaboration coefficient degree was 0.731.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128711019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of retinal capillary nonperfusion in fundus fluorescein angiogram of diabetic retinopathy","authors":"S. Rasta, Shima Nikfarjam, A. Javadzadeh","doi":"10.15171/bi.2015.27","DOIUrl":"https://doi.org/10.15171/bi.2015.27","url":null,"abstract":"Introduction: Retinal capillary nonperfusion (CNP) is one of the retinal vascular diseases in diabetic retinopathy (DR) patients. As there is no comprehensive detection technique to recognize CNP areas, we proposed a different method for computing detection of ischemic retina, non-perfused (NP) regions, in fundus fluorescein angiogram (FFA) images. Methods: Whilst major vessels appear as ridges, non-perfused areas are usually observed as ponds that are surrounded by healthy capillaries in FFA images. A new technique using homomorphic filtering to correct light illumination and detect the ponds surrounded in healthy capillaries on FFA images was designed and applied on DR fundus images. These images were acquired from the diabetic patients who had referred to the Nikookari hospital and were diagnosed for diabetic retinopathy during one year. Our strategy was screening the whole image with a fixed window size, which is small enough to enclose areas with identified topographic characteristics. To discard false nominees, we also performed a thresholding operation on the screen and marked images. To validate its performance we applied our detection algorithm on 41 FFA diabetic retinopathy fundus images in which the CNP areas were manually delineated by three clinical experts. Results: Lesions were found as smooth regions with very high uniformity, low entropy, and small intensity variations in FFA images. The results of automated detection method were compared with manually marked CNP areas so achieved sensitivity of 81%, specificity of 78%, and accuracy of 91%.The result was present as a Receiver operating character (ROC) curve, which has an area under the curve (AUC) of 0.796 with 95% confidence intervals. Conclusion: This technique introduced a new automated detection algorithm to recognize non-perfusion lesions on FFA. This has potential to assist detecting and managing of ischemic retina and may be incorporated into automated grading diabetic retinopathy structures.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133081632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spectroscopic analysis of bosentan in biological samples after a liquid-liquid microextraction","authors":"Sanaz SAJEDI-AMIN, Karim Assadpour-Zeynali, Vahid Panahi-Azar, A. Kebriaeezadeh, M. Khoubnasabjafari, K. Ansarin, V. Jouyban-Gharamaleki, A. Jouyban","doi":"10.15171/bi.2015.28","DOIUrl":"https://doi.org/10.15171/bi.2015.28","url":null,"abstract":"Introduction:Microextraction processes with UV-Vis measurement have been developed and validated for analysis of bosentan in biological samples. Methods:In this work, liquid–liquid microextraction procedures (DLLME & USAEME) were employed for cleanup, pre-concentration, and determination of bosentan in biological samples by UV-Vis spectroscopy at 270 nm. The method was validated and applied to the determination of bosentan in spiked serum, exhaled breath condensate and urine samples. Results:Various experimental factors including type of extraction and dispersive solvents and their volumes, pH, sonication time and centrifuging time were investigated. Under the optimum conditions, the method was linear in the range of 1.0–5.0 μg.mL-1, with coefficient of determination (R2) of > 0.998. The limit of detection (LOD) was 0.07 mg.L-1. Recovery of the target analyte in biological samples was 106.2%. The method could be easily applied for higher concentration of bosentan and needs more improvement for application in the pharmacokinetic investigations where more sensitive methods are required. Conclusion:A simple, low cost, precise and accurate spectrophotometric analysis of bosentan in biological samples after liquid-liquid microextraction were developed and validated for routine analyses.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122484567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem cell therapy of cataract","authors":"M. Maleki","doi":"10.15171/bi.2015.24","DOIUrl":"https://doi.org/10.15171/bi.2015.24","url":null,"abstract":"Introduction: Cataract is recognized as a disease of the lens resulting in many blindness cases, while the only therapeutic procedure is surgery. Thus, to tackle this disease, alternative methods are required. Stem cell therapy is one of the alternative treatment modalities. Paired lens’ epithelial pieces induced by vitreous body were shown to produce lens-like structures. Here, Wharton’s jelly derived stem cells are suggested as the best candidates for this purpose, as these cells have potency for the differentiation into the lens fiber cells. Hypothesis: It is hypothesized that Wharton’s jelly derived stem cells could be used as a novel and appropriate source for the treatment of cataract. Evaluation of Hypothesis: To attain this aim, lens of an animal model of cataract can be removed. Then, the human Wharton’s jelly stem cells (hWJSCs) are injected into a capsule. Finally, the expression of crystalline proteins and vision function are analyzed. Conclusion: It is hypothesized that the lens capsule could act as a natural scaffold and hWJSCs could be used to restore the lens structure in the empty capsule.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"233 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115107441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of orange juice and hesperetin on serum paraoxonase activity and lipid profile in hyperuricemic rats.","authors":"Fatemeh Haidari,&nbsp;Mohammad-Reza Rashidi,&nbsp;Majid Mohammad-Shahi","doi":"10.5681/bi.2012.005","DOIUrl":"https://doi.org/10.5681/bi.2012.005","url":null,"abstract":"<p><strong>Introduction: </strong>Hypouricemic, antioxidant and xanthine oxidase inhibitory effects of orange juice and hesperetin have been already indicated. The objective of this study was to investigate the effects of orange juice and hesperetin on paraoxonase and arylesterase activity and lipid profile of hyperuricemic rats.</p><p><strong>Methods: </strong>Forty eight male Wistar rats were divided into 8 equal groups of healthy control, healthy+orange juice, healthy+hesperetin, healthy+allopurinol, hyperuricemic control, hyperuricemic+orange juice, hyperurice-mic+hesperetin and hyperuricemic+allopurinol. Hyperuricemia was induced using potassi-um oxonate (250 mg/kg ip). The treatments were carried out by daily gavage of 5 ml/kg orange juice, 5 mg/kg hesperetin and 5 mg/kg allopurinol for 2 weeks. Paraoxonase activi-ty in serum was measured spectrophotometrically using paraoxon and phenylacetate as substrates. Serum lipids levels were determined using enzymatic colorimetric methods.</p><p><strong>Results: </strong>Hyperuricemia-induced reduction of paraoxonase and arylesterase activity was restored after treatment with orange juice and hesperetin (p<0.05). The effect of both treatments on lipid profile was marginal and only orange juice could significantly increase the levels of HDL-C.</p><p><strong>Conclusion: </strong>Supplementation of orange juice and hesperetin could restore paraoxonase and arylesterase activity in hyperuricemic rats. Orange juice could also partially improve the lipid profile. These effects could have major implications with respect to the prevention of cardiovascular disease in hyperuricemic patients. However, more studies are needed in future investigations.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"39-45"},"PeriodicalIF":2.6,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5681/bi.2012.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31433793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prophylactic Antibiotic Therapy in Contaminated Traumatic Wounds: Two Days versus Five Days Treatment.","authors":"Hamed-Basir Ghafouri,&nbsp;Barzin Bagheri-Behzad,&nbsp;Mohammad-Reza Yasinzadeh,&nbsp;Ehsan Modirian,&nbsp;Dorsa Divsalar,&nbsp;Shervin Farahmand","doi":"10.5681/bi.2012.004","DOIUrl":"https://doi.org/10.5681/bi.2012.004","url":null,"abstract":"<p><strong>Introduction: </strong>Emergency department manages several kinds of wounds including simple, non-bite traumatic wounds and lacerations. Prophylactic antibiotic therapy is one of pre-scribed treatment in these conditions. We aimed to compare the clinical efficacy of the two day regimen of prophylactic antimicrobial agents with the five day regimen in simple traumatic but highly contaminated wounds.</p><p><strong>Methods: </strong>Between January 2010 and May 2010, patients presenting with simple traumatic wounds or lacerations in different parts of the body, highly contaminated with soil, debris or feces in emergency department of a referral educational hospital in Tehran (Rasul-Akram hospital), Iran, went for primary closure. All of the patients were provided prophylactic antibiotic, however, prescribed for one group (A) of patients for 2 days and other group (B) received for 5 days, according to the physician concerned. As these treatments were routine, we selected 70 patients from each group using table of random numbers. The patients were warned about the signs of infection including long-lasting erythema, purulent discharge and inflammation and were supposed to inform the concerned physician in any of such alarming situations. Oral Cephalexin 500 mg qid was prescribed for all patients enrolled for prophylaxis treatment.</p><p><strong>Results: </strong>On follow-up 11 (8.2%) patients were found to develop sutured site infection (6 out of 70 (8.57%) in group A, and five out of 70 (7.14%) in group B (P=0.31)). There was no statistical difference between infection rates between men (8.6%) in comparison to women (6.25%) (P>0.05; CI=95%).</p><p><strong>Conclusion: </strong>Our study showed that 2-day prophylactic antibiotic therapy using Cephalexin is at least as effective as a 5-day regimen in relation to development of surgical site infection in patients with simple traumatic contaminated wounds or lacerations.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"33-7"},"PeriodicalIF":2.6,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5681/bi.2012.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31433792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting tumor microenvironment: the key role of immune system.","authors":"Jaleh Barar","doi":"10.5681/bi.2012.001","DOIUrl":"https://doi.org/10.5681/bi.2012.001","url":null,"abstract":"<p><p>In recent years, huge investigations on cancer progression and invasion have led to under-stand the pivotal role of tumor microenvironment. The current era of cancer therapy is based on the concept of simply targeting precise mechanisms to kill or to suppress the growth and expansion of malignant cells. Clinical data clearly correlate with in-vitro re-sults, emphasizing the direct impact of cancer environment on disease progression. This provides the opportunity to advance cancer therapy by virtue of targeting cancerous cells and non-cancerous component of tumor in a combinatorial manner. This tailor-made strategy demands the profound knowledge of cross talk between the bio-factors of tumor environment and corresponding pharmacology of drug candidates. The neighborhood of tumor is critical for how cancer cells grow and invade surrounding tissues. It appears that the tumor microenvironment as a \"co-op\" includes malignant cells, blood vessels, im-mune/inflammatory factors and extracellular matrix. As a longstanding dilemma, it is well-proved that immune system plays a direct role in the existence and progression of such coop. In some cases, immune cells e.g. tumor associated macrophages (TAMs) infiltrate into tumor and instead of fighting cancer cells, support them to grow. As an important fact, this tumor complexity should not be taken as granted where it can be advantageous in cancer therapy as well as early detection and prevention. The central aim of this editorial article is to highlight the importance of tumor microenvironment for successful cancer therapy.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"1-3"},"PeriodicalIF":2.6,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5681/bi.2012.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31434370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promoters of cancer genes for recombinant protein expression in human cancer cell lines.","authors":"Mohammad Pourhassan-Moghaddam,&nbsp;Behrouz Farhadi,&nbsp;Kazem Nejati-Koshki,&nbsp;Tamouchin Moharrami","doi":"10.5681/bi.2012.007","DOIUrl":"https://doi.org/10.5681/bi.2012.007","url":null,"abstract":"<p><strong>Introduction: </strong>Production of complex human recombinant proteins is an important issue in medical biotechnology. These proteins are mostly expressed in non-human mammalian host cells. This has some problems including non-human post-translational modifications, application of high-cost agents for inducing protein expression and low yields. Thus, it is necessary to use new expression systems to overcome the indicated challenges.</p><p><strong>Methods: </strong>In this paper, we hypothesize the application of promoter regions of cancer genes, which have a high rate of transcription in human cancer cell lines, for designing new expression vectors.</p><p><strong>Results: </strong>After designing, these vectors could be applied to produce complex hu-man recombinant proteins in the human cancer cell lines as production hosts.</p><p><strong>Conclusion: </strong>Application of these expression vectors for the production of recombinant human proteins in the human cancer cell lines have some advantages including authentic post-translational modifications, proper-cost of commercialization, and high yields.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"55-60"},"PeriodicalIF":2.6,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5681/bi.2012.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31433795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}