实验室环境与生物医学经验:给药技术对免疫行为数据质量的影响导致应激经验

Nessaibia Issam, T. Abdelkrim, Chouba Ibtissem, Kaarar Narjess
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引用次数: 2

摘要

引言:通常在实验中,控制组和完整组是不确定的,因为大多数科学家忽视了这样一个事实,即作为技术管理的操作集可能被认为是对科学研究中获得的数据的清晰度的不良压力,特别是如果它侧重于研究压力的影响。方法:采用雄性Wistar大鼠40只,分为两部分进行研究。第一部分以1 mL/kg剂量的安慰剂或0.9%的氯化钠(NaCl)反复注射,另一部分以0.9%的氯化钠(NaCl)直接口服。两组均以应激组进行1 h的喷射空气应激。我们的目的是考虑在这种应激体验中,这些操作对行为结果(升高加迷宫测试、开阔场地、光/暗盒测试)和免疫数据(免疫细胞计数)的有效性的影响。第二部分为实验组ACTH、il - 6、CRP的测定。结果:与口服给药不同,反复腹腔注射安慰剂:氯化钠0.9% (1 mL/kg)可导致血浆促肾上腺皮质激素(ACTH)、白细胞介素-6 (IL-6)和c反应蛋白(CRP)水平显著升高,并可能对白细胞配方和行为试验确定的动物笼中空气喷射1小时引起的显著免疫和行为改变数据质量产生副作用。结论:在对动物模型进行的实验方案中,选择无痛技术如口服给药而不是疼痛注射是必要的,以避免在生物医学实验中行为和免疫结果的混淆,特别是在应激研究中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Laboratory environment and bio-medical experience: the impact of administration technique on the quality of immune-behavior data results in stress experience
Introduction: Often in an experiment, the control group and the intact group are not identified because most scientists neglect the fact that the sets of manipulation as technical administrations may be considered as an undesirable stress on the clarity of the data obtained from a scientific research specifically if it focuses on studying the effects of stress. Methods: This study was conducted in two parts using 40 male Wistar rats. The first part aimed to treat a group of rats by repeated injections i.p route (1 mL/kg) of placebo or NaCl (0.9%) and the other by direct oral administration of NaCl (0.9%). Both groups spent 1 h of jet air stress with stressed group. Our objective was to consider the effects that these manipulations would have on the validity of behavioral results (the elevated plus maze test, the open field, the light/dark box test) and immune data (immune cell count) during this stress experience. The second part was devoted to the measurement of ACTH, IL6, and CRP in these experimental groups. Results: Unlike oral administration, repeated intra-peritoneal injections cause a significant increase of plasma obtained levels of the adrenocorticotropin hormone (ACTH), interleukin-6 (IL-6) and the C-reactive protein (CRP) using injections of placebo: NaCl 0.9% (1 mL/kg) and it may have side effect on significant immune and behavioral alterations data quality induced by 1 h of air jet in the animal’s cage identified by the leukocyte formula and behavioral tests. Conclusion: In an experimental protocol conducted on animal models, it is essential to opt for painless techniques such as oral administration instead of painful injections to avoid confusion at the behavioral and immunological results from biomedical experiments specifically one that focuses on the stress study.
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