BioImpacts : BI最新文献

{"title":"An opinion on the impacts of COVID-19 worldwide","authors":"R. Speth","doi":"10.34172/bi.2022.24124","DOIUrl":"https://doi.org/10.34172/bi.2022.24124","url":null,"abstract":"","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"94 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122415748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Opioid overdose, interventions, and challenges","authors":"S. Alaei, H. Omidian","doi":"10.34172/bi.2022.24093","DOIUrl":"https://doi.org/10.34172/bi.2022.24093","url":null,"abstract":"Summary This short letter briefly reviews the significance of opioid overdose as well as the existing approaches to combat the epidemic. It also signifies the importance of naloxone, its commercially available dosage forms, and the unmet need for developing safe and effective naloxone dosage forms that can easily be administered in emergency settings.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125321004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advancements in cell-based models for auditory disorders","authors":"J. Langlie, Ariel Finberg, N. Bencie, Jeenu Mittal, H. Omidian, Y. Omidi, R. Mittal, A. Eshraghi","doi":"10.34172/bi.2022.23900","DOIUrl":"https://doi.org/10.34172/bi.2022.23900","url":null,"abstract":"Introduction: Cell-based models play an important role in understanding the pathophysiology and etiology of auditory disorders. For the auditory system, models have primarily focused on restoring inner and outer hair cells. However, they have largely underrepresented the surrounding structures and cells that support the function of the hair cells. Methods: In this article, we will review recent advancements in the evolution of cell-based models of auditory disorders in their progression towards three dimensional (3D) models and organoids that more closely mimic the pathophysiology in vivo. Results: With the elucidation of the molecular targets and transcription factors required to generate diverse cell lines of the components of inner ear, research is starting to progress from two dimensional (2D) models to a greater 3D approach. Of note, the 3D models of the inner ear, including organoids, are relatively new and emerging in the field. As 3D models of the inner ear continue to evolve in complexity, their role in modeling disease will grow as they bridge the gap between cell culture and in vivo models. Conclusion: Using 3D cell models to understand the etiology and molecular mechanisms underlying auditory disorders holds great potential for developing more targeted and effective novel therapeutics.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115815157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes as versatile nanoscaled biocompartments in cancer therapy and/or resistance","authors":"Yalda Rahbar Saadat, J. Barar","doi":"10.34172/bi.2022.24253","DOIUrl":"https://doi.org/10.34172/bi.2022.24253","url":null,"abstract":"Summary Cancer remains to be a major hurdle to global health. Exosomes as a versatile bio-derived platform, hold a bright prospect in nano-scaled delivery/targeting strategies. Shreds of evidence indicate that exosomes have a critical role in drug resistance in cancer cells through various mechanisms including shuttling of miRNAs, drug efflux transporters, and anti-apoptotic signaling. Exosomes’ cargo, particularly miRNAs, may exert both resistance and in a few cases sensitivity to the anticancer agents in targeted cells. Therefore, the source and components of the exosomes should be carefully considered before any application. Our aim in this editorial is to further highlight the role of exosomes in the development of resistance to therapy in cancer cells. As a new chapter for drug delivery, the challenges should be elucidated before exosomes emerge as novel nanoplatforms for cancer therapy.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"113 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124278596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cholinergic anti-inflammatory pathway and COVID-19","authors":"D. Mehranfard, R. Speth","doi":"10.34172/bi.2022.23980","DOIUrl":"https://doi.org/10.34172/bi.2022.23980","url":null,"abstract":"The cholinergic anti-inflammatory pathway (CAP) first described by Wang et al, 2003 has contemporary interest arising from the COVID-19 pandemic. While tobacco smoking has been considered an aggravating factor in the severity of COVID-19 infections, it has been suggested by some that the nicotine derived from tobacco could lessen the severity of COVID-19 infections. This spotlight briefly describes the CAP and its potential role as a therapeutic target for the treatment of COVID-19 infections using vagus nerve stimulation or selective alpha7 nicotinic acetylcholine receptor agonists.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121506742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"To detect potential pathways and target genes in infantile Pompe patients using computational analysis","authors":"Aynur Karadağ Gürel, S. Gürel","doi":"10.34172/bi.2022.23467","DOIUrl":"https://doi.org/10.34172/bi.2022.23467","url":null,"abstract":"Introduction: Pompe disease (PD) is a disease caused by pathogenic variations in the GAA gene known as glycogen storage disease type II, characterized by heart hypertrophy, respiratory failure, and muscle hypotonia, leading to premature death if not treated early. The only treatment option, enzyme replacement therapy (ERT), significantly improves the prognosis for some patients while failing to help others. In this study, the determination of key genes involved in the response to ERT and potential molecular mechanisms were investigated. Methods: Gene Expression Omnibus (GEO) data, accession number GSE38680, containing samples of biceps and quadriceps muscles was used. Expression array data were analyzed using BRB-Array Tools. Biceps group patients did not receive ERT, while quadriceps received treatment with rhGAA at 0, 12, and 52 weeks. Differentially expressed genes (DEGs) were deeply analyzed by DAVID, GO, KEGG and STRING online analyses, respectively. Results: A total of 1727 genes in the biceps group and 1198 genes in the quadriceps group are expressed differently. It was observed that DEGs were enriched in the group that responded poorly to ERT in the 52nd week. Genes frequently changed in the weak response group; the expression of 530 genes increased and 1245 genes decreased compared to 0 and 12 weeks. The GO analysis demonstrated that the DEGs were mainly involved in vascular smooth muscle contraction, lysosomes, autophagy, regulation of actin cytoskeleton, inflammatory response, and the WNT signaling pathway. We also discovered that the WNT signaling pathway is highly correlated with DEGs. Several DEGs, such as WNT11, WNT5A, CTNNB1, M6PR, MYL12A, VCL, TLN, FYN, YES1, and BCL2, may be important in elucidating the mechanisms underlying poor response to ERT. Conclusion: Early diagnosis and treatment of PD are very important for the clinic of the disease. As a result, it suggests that the enriched genes and new pathways emerging as a result of the analysis may help identify the group that responds poorly to treatment and the outcome of the treatment. Obtained genes and pathways in neonatal screening will guide diagnosis and treatment.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128832275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CRISPRi-mediated knock-down of PRDM1/BLIMP1 programs central memory differentiation in <i>ex vivo</i>-expanded human T cells.","authors":"Mohammad Azadbakht,&nbsp;Ali Sayadmanesh,&nbsp;Naghme Nazer,&nbsp;Amirhossein Ahmadi,&nbsp;Sara Hemmati,&nbsp;Hoda Mohammadzade,&nbsp;Marzieh Ebrahimi,&nbsp;Hossein Baharvand,&nbsp;Babak Khalaj,&nbsp;Mahmoud Reza Aghamaali,&nbsp;Mohsen Basiri","doi":"10.34172/bi.2021.23522","DOIUrl":"https://doi.org/10.34172/bi.2021.23522","url":null,"abstract":"<p><p><i><b>Introduction:</b> </i> B lymphocyte-induced maturation protein 1 (BLIMP1) encoded by the positive regulatory domain 1 gene (<i>PRDM1</i>), is a key regulator in T cell differentiation in mouse models. BLIMP1-deficiency results in a lower effector phenotype and a higher memory phenotype. <i><b>Methods:</b> </i> In this study, we aimed to determine the role of transcription factor BLIMP1 in human T cell differentiation. Specifically, we investigated the role of BLIMP1 in memory differentiation and exhaustion of human T cells. We used CRISPR interference (CRISPRi) to knock-down BLIMP1 and investigated the differential expressions of T cell memory and exhaustion markers in BLIMP1-deficient T cells in comparison with BLIMP1-sufficient ex vivo expanded human T cells. <i><b>Results:</b> </i> BLIMP1-deficiency caused an increase in central memory (CM) T cells and a decrease in effector memory (EM) T cells. There was a decrease in the amount of TIM3 exhaustion marker expression in BLIMP1-deficient T cells; however, there was an increase in PD1 exhaustion marker expression in BLIMP1-deficient T cells compared with BLIMP1-sufficient T cells. <i><b>Conclusion:</b> </i> Our study provides the first functional evidence of the impact of BLIMP1 on the regulation of human T cell memory and exhaustion phenotype. These findings suggest that BLIMP1 may be a promising target to improve the immune response in adoptive T cell therapy settings.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"337-347"},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/85/3c/bi-12-337.PMC9376159.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40420451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COVID-19: Artificial sputum, respiratory obstruction method and screening of pyolitic and antihypoxic drugs.","authors":"Aleksandr L Urakov,&nbsp;Natalya A Urakova,&nbsp;Ilnur I Yagudin,&nbsp;Milena D Svetova,&nbsp;Darya O Suntsova","doi":"10.34172/bi.2022.23877","DOIUrl":"https://doi.org/10.34172/bi.2022.23877","url":null,"abstract":"<p><p>COVID-19 causes non-specific pneumonia, which has become a new cause of hypoxia, leading to the death of many patients. Today, there are no effective drugs that provide an urgent increase in blood oxygenation. Therefore, it is urgently necessary to develop drugs to increase blood oxygenation in order to save the lives of patients with the new coronavirus infection. Since hypoxia develops in this disease due to the blockage of respiratory tract with viscous mucus and sputum, an appropriate experimental model is needed for screening and finding new drugs. However this model is yet missing. Therefore, the development of an experimental model of respiratory obstruction by sputum with traces of blood can accelerate the discovery of drugs that eliminate hypoxia and prevent the death of patients with nonspecific pneumonia complicated by respiratory obstruction. The purpose of this letter was to present a model for evaluating the biological activity of drugs, which can become a new vector for the development of effective ways to increase blood oxygenation across pulmonary and save the lives of patients with severe atypical pneumonia complicated by respiratory obstruction in COVID-19.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"393-394"},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7e/7d/bi-12-393.PMC9376158.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40420453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intra-ovarian injection of bone marrow-derived c-Kit⁺ cells for ovarian rejuvenation in menopausal rats.","authors":"Sepideh Sheshpari,&nbsp;Mahnaz Shahnazi,&nbsp;Shahin Ahmadian,&nbsp;Mohammad Nouri,&nbsp;Mehran Mesgari Abbasi,&nbsp;Rahim Beheshti,&nbsp;Reza Rahbarghazi,&nbsp;Ali Honaramooz,&nbsp;Mahdi Mahdipour","doi":"10.34172/bi.2021.23499","DOIUrl":"https://doi.org/10.34172/bi.2021.23499","url":null,"abstract":"<p><p><i><b>Introduction:</b> </i> Cell-based therapies with certain cell types are touted as novel and hopeful therapeutic intervention in the clinical setting. Here, we aimed to assess the regenerative potential of c-Kit⁺ cells in the rejuvenation of ovarian tissue and fertility rate in rat model of premature ovarian failure (POF). <i><b>Methods:</b> </i> Rats were treated with 160 mg/kg/BW of 4-vinylcyclohexene dioxide for 15 days. Freshly enriched rat bone marrow-derived c-Kit⁺ (MACS) and c-Kit^- cells (4×10⁵ cells/10 µL) were transplanted into the ovaries of treatment and control animals. Prior to transplantation as well as 2, 4, 6, and 8 weeks post-transplantation, randomly-selected rats were euthanized and ovarian tissues were subjected to pathophysiological examinations and real-time PCR analyses. <i><b>Results:</b> </i> POF status was confirmed by the presence of pathological features and a decreased number of immature and mature follicles compared with the control group (<i>P </i>< 0.05). Histological examination revealed a substantial reduction of atretic follicles in POF rats receiving c-Kit⁺ cells in comparison with POF rats that did not receive these cells (<i>P </i>< 0.05). Compared with the control samples, angiogenesis-related genes, <i>Angpt2</i> and <i>KDR</i>, showed increased and decreased expressions in POF ovaries, respectively (<i>P </i>< 0.05). c-Kit⁺ cells had potential to restore angiogenesis in the ovarian tissue within normal ranges. Systemic levels of FSH did not significantly change in pre- or post-transplantation time points for any group (<i>P </i>> 0.05). Notable reduction of collagen deposition was found in c-Kit-treated rats. Transplantation of c-Kit⁺ cells also restored the reduced fertility rate (<i>P </i>< 0.05). <i><b>Conclusion:</b> </i> The administration of c-Kit⁺ cells can modulate angiogenesis and pathological changes, leading to the rejuvenation of ovarian function of a rat model of premature menopause.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"325-335"},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/24/13/bi-12-325.PMC9376162.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40420449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optogenetics: A new tool for cancer investigation and treatment.","authors":"Siamak Alizadeh,&nbsp;Abolghasem Esmaeili,&nbsp;Jaleh Barar,&nbsp;Yadollah Omidi","doi":"10.34172/bi.2021.22179","DOIUrl":"https://doi.org/10.34172/bi.2021.22179","url":null,"abstract":"<p><p>Despite the progress made in the diagnosis and treatment of cancer, it has remained the second cause of death in industrial countries. Cancer is a complex multifaceted disease with unique genomic and proteomic hallmarks. Optogenetics is a biological approach, in which the light-sensitive protein modules in combination with effector proteins that trigger reversibly fundamental cell functions without producing a long-term effect. The technology was first used to address some key issues in neurology. Later on, it was also used for other diseases such as cancer. In the case of cancer, there exist several signaling pathways with key proteins that are involved in the initiation and/or progression of cancer. Such aberrantly expressed proteins and the related signaling pathways need to be carefully investigated in terms of cancer diagnosis and treatment, which can be managed with optogenetic tools. Notably, optogenetics systems offer some advantages compared to the traditional methods, including spatial-temporal control of protein or gene expression, cost-effective and fewer off-target side effects, and reversibility potential. Such noticeable features make this technology a unique drug-free approach for diagnosis and treatment of cancer. It can be used to control tumor cells, which is a favorable technique to investigate the heterogeneous and complex features of cancerous cells. Remarkably, optogenetics approaches can provide us with outstanding tool to extend our understanding of how cells perceive, respond, and behave in meeting with complex signals, particularly in terms of cancer evasion from the anticancer immune system functions.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"295-299"},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b7/70/bi-12-295.PMC9376163.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40617281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}