BioImpacts : BI最新文献

{"title":"High efficacy of tamoxifen-loaded L-lysine coated magnetic iron oxide nanoparticles in cell cycle arrest and anti-cancer activity for breast cancer therapy.","authors":"Soheila Rostami,&nbsp;Farzaneh Tafvizi,&nbsp;Hamid Reza Kheiri Manjili","doi":"10.34172/bi.2021.23337","DOIUrl":"https://doi.org/10.34172/bi.2021.23337","url":null,"abstract":"<p><p><i><b>Introduction:</b></i> Due to the side effects of drugs, the development of nanoscale drug delivery systems has led to a significant improvement in medicinal therapies due to drug pharmacokinetics changes, decreased toxicity, and increased half-life of the drug. This study aimed to synthesize tamoxifen (TMX)-loaded L-lysine coated magnetic iron oxide nanoparticles as a nano-carrier to investigate its cytotoxic effects and anti-cancer properties against MCF-7 cancer cells. <i><b>Methods:</b></i> Magnetic Fe₃O₄ nanoparticles were synthesized and coated with L-lysine (F-Lys NPs). Then, TMX was loaded onto these NPs. The characteristics of synthesized nanoparticles (F-Lys-TMX NPs) were evaluated by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), differential scanning calorimetry (DSC), vibrating sample magnetometer (VSM), and thermogravimetric analysis (TGA). The drug release was analyzed at pH 5.8 and pH 7.4. The MCF-7 cells were exposed to F-Lys-TMX NPs, F-Lys NPs, and TMX for 24, 48, and 72 hours. To evaluate the cytotoxic potential of designed nanoparticles, MTT and apoptosis assays, real-time PCR, and cell cycle analysis was carried out. <i><b>Results:</b></i> The F-Lys-TMX NPs had spherical morphology with a size ranging from 9 to 30 nm. By increasing the nanoparticles concentration and treatment time, more cell proliferation inhibition and apoptosis induction were observed in F-Lys-TMX NPs-treated cells compared to the TMX. The expression levels of ERBB2, cyclin D1, and cyclin E genes were down-regulated and expression levels of the caspase-3 and caspase-9 genes were up-regulated. Studies on the drug release revealed a slow and controlled pH-dependent release of the nanoparticles. Cell cycle analysis indicated that F-Lys-TMX NPs could arrest the cells at the G0/G1 phase. <i><b>Conclusion:</b></i> The findings suggest that F-Lys-TMX NPs are more effective and have the potential for cell proliferation inhibition and apoptosis induction compared to the TMX. Hence, F-Lys-TMX NPs can be considered as an anti-cancer agent against MCF-7 breast cancer cells.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"301-313"},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a9/ab/bi-12-301.PMC9376161.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40704044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic effects of silver nanoparticle and L-carnitine on aerobic vaginitis in mice: an experimental study.","authors":"Mozhgan Fatahi Dehpahni,&nbsp;Khosrow Chehri,&nbsp;Mehri Azadbakht","doi":"10.34172/bi.2021.22037","DOIUrl":"https://doi.org/10.34172/bi.2021.22037","url":null,"abstract":"<p><p><i><b>Introduction:</b></i> Aerobic vaginitis (AV) is a type of vaginal infection that occurs at the reproductive age of women. In this study, we aimed to study the possible anti-AV therapeutic effects of silver nanoparticles (AgNPs) and L-carnitine (LC) in the mouse model. <i><b>Methods:</b></i> AV model was established by intra-vaginal inoculation of 10⁸ CFU/mL <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> (1:1) in adult NMRI mice. Susceptibilities of the bacteria were examined against AgNPs by inhibitory concentration (IC-50 and IC-90) and minimum biofilm inhibitory concentration (MBIC- 90) methods. The regimens therapy was intra-vaginal inoculation of AgNPs at MBIC- 90 and a daily injection of 250 mg/kg LC for two weeks. Mice were classified into healthy (control) and AV groups and then treated by LC, AgNPs, and AgNPs + LC. The vaginal smears were taken daily and tissue sections were prepared using the hematoxylin and eosin (H & E) method. <i><b>Results:</b></i> Minimum inhibitory concentrations (MICs) of AgNPs for <i>E. coli, S. aureus,</i> and their mixture were 250, 125, and 500 ppm, and their MBIC-90% were 500, 250, and 1000 ppm, respectively. The estrus cycle of mice treated with co-administration of AgNPs and LC was similar to the control group (<i>P</i> < 0.05). The results of histology also showed that infected mice were treated with AgNPs and LC, simultaneously. <i><b>Conclusion:</b></i> Single bacteria are more sensitive than their mixed model to these NPs. Co-administration of AgNPs as an antibacterial agent and LC as an antioxidant agent can treat AV in the infected mice.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"33-42"},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/60/a3/bi-12-33.PMC8783078.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39865408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-1290 contributes to oncogenesis and angiogenesis via targeting of THBS1, DKK3 and, SCAI.","authors":"Mohammad Hasan Soheilifar,&nbsp;Majid Pornour,&nbsp;Massoud Saidijam,&nbsp;Rezvan Najafi,&nbsp;Farid Azizi Jalilian,&nbsp;Hoda Keshmiri Neghab,&nbsp;Razieh Amini","doi":"10.34172/bi.2021.23571","DOIUrl":"https://doi.org/10.34172/bi.2021.23571","url":null,"abstract":"<p><p><i><b>Introduction:</b> </i> Colorectal cancer (CRC) is the third most common cancer in the world with high mortality, hence, understanding the molecular mechanisms involved in the tumor progression are important for CRC diagnosis and treatment. MicroRNAs (miRNAs) are key gene expression regulators that can function as tumor suppressors or oncogenes in tumor cells, and modulate angiogenesis as a critical process in tumor metastasis. MiR-1290 has been demonstrated as an onco-miRNA in various types of cancer, however, the role of miR-1290 in CRC is not fully understood. This study aimed to investigate the oncogenic and angiogenic potential of miR-1290 in CRC. <i><b>Methods:</b> </i> Lenti-miR-1290 was transduced into HCT116, SW480, and human umbilical vein endothelial cells (HUVECs). By bioinformatics analysis, we identified thrombospondin 1 (THBS1) as a novel predicted target for miR-1290. Quantitative real-time PCR, western blotting, and luciferase reporter assay were used to demonstrate suppression of miR-1290 target genes including THBS1, Dickkopf Wnt signaling pathway inhibitor 3 (DKK3), and suppressor of cancer cell invasion (SCAI) in HCT116 and HUVECs. Cell cycle analysis, proliferation, migration and, tube formation were determined by flow cytometry, MTT, wound healing, and tube formation assays, respectively. <i><b>Results:</b> </i> MiR-1290 significantly decreased the expression of THBS1, DKK3, and SCAI. We demonstrated that miR-1290 enhanced proliferation, migration, and angiogenesis partially through suppression of THBS1, DKK3, and SCAI in CRC. <i><b>Conclusion:</b> </i> These results suggest a novel function of miR-1290 which may contribute to tumorigenesis and angiogenesis in CRC.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"349-358"},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/18/41/bi-12-349.PMC9376166.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40420450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Krabbe disease: A personal perspective and hypothesis.","authors":"Mohammad A Rafi","doi":"10.34172/bi.2021.23931","DOIUrl":"https://doi.org/10.34172/bi.2021.23931","url":null,"abstract":"<p><p><i><b>Introduction:</b></i> Krabbe disease (KD) or globoid cell leukodystrophy (GLD) is one of the lysosomal disorders affecting central and peripheral nervous systems (CNS and PNS). It is caused by mutations on the galactocerebrosidase (GALC) gene. Affected individuals accumulate undegraded substrates and suffer from neuroinflammation. <i><b>Methods:</b></i> Hematopoietic stem cell transplantation (HSCT) has been partially successful in treating patients with KD when accomplished prior to the onset of symptoms. The success is credited to the ability of the hematopoietic stem cells in providing some GALC enzyme to the CNS and eradicating potential neuroinflammation. Combination of the HSCT with some other GALC-providing strategies has shown synergistic effects in the treatment of the mouse model of this disease. <i><b>Results:</b></i> Here, the possibility of eliminating HSCT in the treatment of human patients and replacing it with a single therapy that will provide sufficient GALC enzyme to the nervous systems is suggested. Such treatment, if started during the asymptomatic stage of the disease, not only may eradicate the enzyme deficiency, but may also keep any neuroinflammation at bay. <i><b>Conclusion:</b></i> Successful treatment of the KD may be possible by restoring consistent and sufficient GALC expression in CNS and PNS.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"3-7"},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/31/77/bi-12-3.PMC8783082.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39865406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advance trends in targeting homology-directed repair for accurate gene editing: An inclusive review of small molecules and modified CRISPR-Cas9 systems.","authors":"Forough Shams,&nbsp;Hadi Bayat,&nbsp;Omid Mohammadian,&nbsp;Somayeh Mahboudi,&nbsp;Hassan Vahidnezhad,&nbsp;Mohsen Soosanabadi,&nbsp;Azam Rahimpour","doi":"10.34172/bi.2022.23871","DOIUrl":"https://doi.org/10.34172/bi.2022.23871","url":null,"abstract":"<p><p><i><b>Introduction:</b> </i> Clustered regularly interspaced short palindromic repeat and its associated protein (CRISPR-Cas)-based technologies generate targeted modifications in host genome by inducing site-specific double-strand breaks (DSBs) that can serve as a substrate for homology-directed repair (HDR) in both <i>in vitro</i> and <i>in vivo</i> models. HDR pathway could enhance incorporation of exogenous DNA templates into the CRISPR-Cas9-mediated DSB site. Owing to low rate of HDR pathway, the efficiency of accurate genome editing is diminished. Enhancing the efficiency of HDR can provide fast, easy, and accurate technologies based on CRISPR-Cas9 technologies. <i><b>Methods:</b> </i> The current study presents an overview of attempts conducted on the precise genome editing strategies based on small molecules and modified CRISPR-Cas9 systems. <i><b>Results:</b> </i> In order to increase HDR rate in targeted cells, several logical strategies have been introduced such as generating CRISPR effector chimeric proteins, anti-CRISPR proteins, modified Cas9 with donor template, and using validated synthetic or natural small molecules for either inhibiting non-homologous end joining (NHEJ), stimulating HDR, or synchronizing cell cycle. Recently, high-throughput screening methods have been applied for identification of small molecules which along with the CRISPR system can regulate precise genome editing through HDR. <i><b>Conclusion:</b> </i> The stimulation of HDR components or inhibiting NHEJ can increase the accuracy of CRISPR-Cas-mediated engineering systems. Generating chimeric programmable endonucleases provide this opportunity to direct DNA template close proximity of CRISPR-Cas-mediated DSB. Small molecules and their derivatives can also proficiently block or activate certain DNA repair pathways and bring up novel perspectives for increasing HDR efficiency, especially in human cells. Further, high throughput screening of small molecule libraries could result in more discoveries of promising chemicals that improve HDR efficiency and CRISPR-Cas9 systems.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"371-391"},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b9/de/bi-12-371.PMC9376165.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40420448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing multi-epitope based peptide vaccine candidates against SARS-CoV-2 using immunoinformatics approach.","authors":"Ysrafil Ysrafil,&nbsp;Zulfiayu Sapiun,&nbsp;Indwiani Astuti,&nbsp;Mohammad Anas Anasiru,&nbsp;Nangsih Sulastri Slamet,&nbsp;Hartati Hartati,&nbsp;Fadli Husain,&nbsp;Sukmawati Ahmad Damiti","doi":"10.34172/bi.2022.23769","DOIUrl":"https://doi.org/10.34172/bi.2022.23769","url":null,"abstract":"<p><p><i><b>Introduction:</b> </i> The current incidence of the novel coronavirus disease has shown only small reductions of cases and has become a major public health challenge. Development of effective vaccines against the virus is still being encouraged such as multi-epitope vaccines designed from the components of SARS-CoV-2 including its spike, nucleocapsid and ORF1a proteins. Since the addition of adjuvants including HABA protein and L7/L12 ribosomal are considered helpful to increase the effectiveness of the designed vaccine, we proposed to design multiepitope vaccines by two different adjuvants. <i><b>Methods:</b> </i> We used the IEDB server to predict BCL and TCL epitopes that were characterized using online tools including VaxiJen, AllPred and IL-10 Prediction. The selected epitopes were further constructed into multiepitope vaccines. We also added two different adjuvants to the vaccine components in order to increase the effectiveness of the vaccines. The 3D-structured vaccines were built using trRosetta. They were further docked with different Toll-like-receptors (TLR 3, 4 and 8) and the entry receptor of SARS-CoV-2, ACE2 using ClusPro, PatchDock and refined by FireDock. All structures were visualized by USCF Chimera and PyMOL. <i><b>Results:</b> </i> In this study, we succeeded in designing two different candidate vaccines by the addition of HABA protein and L7/L12 ribosomal as adjuvants. The two vaccines were almost equally good in terms of their physicochemical properties and characteristics. Likewise, their strong interactions with TLR3 4, 8 and ACE2 show the lowest energy level of both was estimated at more than -1,000. Interactions of vaccines with ACE2 and TLRs are essential for activation of immune responses and production of antibodies. <i><b>Conclusion:</b> </i> The two designed and constructed multiepitope vaccine have good characteristics and may have the potential to activate humoral and cellular immune responses against SARS-CoV-2. Further research is worth considering to confirm the findings of this study.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"359-370"},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/40/e6/bi-12-359.PMC9376164.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40420452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simple monitoring of pH and urea in whole blood using wearable smart woman pad.","authors":"Bambang Kuswandi,&nbsp;Nur Andriani,&nbsp;Ari S Nugraha","doi":"10.34172/bi.2021.41","DOIUrl":"https://doi.org/10.34172/bi.2021.41","url":null,"abstract":"<p><p><i><b>Introduction:</b></i> In this work, we used a thread-paper microfluidic device (μTPAD) system, where a threaded part for the handling of the whole blood samples and a paper part for the reaction of plasma with immobilized bioreagents integrated into woman pad as a wearable sensing device namely as smart women pad. The μTPAD as a wearable smart woman pad is developed for the detection of pH and urea in mensuration blood as real samples. <i><b>Methods:</b></i> This combined device was constructed to cover the elements required, that is, separation of red blood cell, conditioning, analyte reaction, and colorimetric detection. The color change in sensing areas was measured in the RGB values via a smartphone using the Color Grab after a smart woman pad was used. The thread allowed red blood cell sampling and separation, while the paper microfluidic device was used for conditioning, biorecognition, and colorimetric transduction of pH and urea as analytes. <i><b>Results:</b></i> The time needed for analysis was measured as 110 s using the equilibrium method for both analytes, with a limit of detection (LOD) of 72.55 μg/mL for urea, with precision around 1.68%, while for pH around 0.80%. The smart woman pad allowed rapid detection of pH and urea in menstruation blood as real samples for monitoring of the kidney functions, and the results showed an agreement with the conventional methods that have been generally used in the clinical laboratory. <i><b>Conclusion:</b></i> The smart woman pad has the potential to be used as a wearable device to monitor the health status of the user via its blood mensuration analysis.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"43-50"},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a4/cd/bi-12-43.PMC8783083.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39865409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiviral and anti-inflammatory drugs to combat COVID-19: Effects on cardiac ion channels and risk of ventricular arrhythmias.","authors":"Luigi X Cubeddu,&nbsp;Daisy de la Rosa,&nbsp;Michele Ameruoso","doi":"10.34172/bi.2021.23630","DOIUrl":"https://doi.org/10.34172/bi.2021.23630","url":null,"abstract":"<p><p><i><b>Introduction:</b></i> Drugs with no indication for the treatment of cardiovascular diseases (e.g., drugs employed to treat COVID-19) can increase the risk of arrhythmias. Of interest, a six-fold increase in the number of arrhythmic events was reported in patients with severe COVID-19. In this study, we reviewed (i) the pro-arrhythmic action of drugs given to patients with COVID-19 infection, and (ii) the effects of inflammatory cytokines on cardiac ion channels and possible generation of arrhythmias. <i><b>Methods:</b></i> We conducted a literature search on the drugs with purported or demonstrated efficacy against COVID-19 disease, emphasizing the mechanisms by which anti-COVID-19 drugs and inflammatory cytokines interfere with cardiac ion channels. <i><b>Results:</b></i> Antibiotics (azithromycin), antimalarials (hydroxychloroquine, chloroquine), antivirals (ritonavir/lopinavir, atazanavir), and some of the tyrosine kinase inhibitors (vandetanib) could induce long QT and increase risk for ventricular arrhythmias. The pro-arrhythmic action results from drug-induced inhibition of Kv11.1 (hERG) channels interfering with the repolarizing potassium <i>IKr</i> currents, leading to long QT and increased risk of triggered arrhythmias. At higher concentrations, these drugs may interfere with <i>IKs</i>, <i>IK1</i>, and/or Ito potassium currents, and even inhibit sodium (<i>INa</i>) and calcium (<i>ICa</i>) currents, inducing additional cardiac toxicity. Ibrutinib, an inhibitor of Bruton's TK, increased the incidence of atrial fibrillation and ventricular tachycardia associated with a short QT interval. Inflammatory cytokines IL-6 and TNF-α inhibit <i>IKr</i> and Ito repolarizing potassium currents. High levels of inflammatory cytokines could contribute to the arrhythmic events. For remdesivir, favipiravir, dexamethasone, tocilizumab, anakinra, baricitinib, and monoclonal antibodies (bamlanivimab, etesevimab, and casirivimab), no evidence supports significant effects on cardiac ion channels, changes in the QT interval, and increased risk for ventricular arrhythmias. <i><b>Conclusion:</b></i> This study supports the concept of hERG channel promiscuity. Different drug classes given to COVID-19 patients might delay repolarization, and increase the risk of ventricular arrhythmias. The presence of comorbid pro-arrhythmic disease states, and elevated levels of pro-arrhythmic cytokines, could increase the risk of ventricular arrhythmias. Discontinuation of nonessential drugs and correction of electrolyte abnormalities could prevent severe ventricular arrhythmias. Altogether, the most effective therapies against COVID-19 (remdesivir, dexamethasone, monoclonal antibodies) lack pro-arrhythmic activity.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"9-20"},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ea/7f/bi-12-9.PMC8783084.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39865407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesenchymal stromal cell therapy alone does not lead to complete restoration of skin parameters in diabetic foot patients within a 3-year follow-up period.","authors":"Nadezhda V Maksimova,&nbsp;Anna V Michenko,&nbsp;Olga A Krasilnikova,&nbsp;Ilya D Klabukov,&nbsp;Igor Yu Gadaev,&nbsp;Michael E Krasheninnikov,&nbsp;Pavel A Belkov,&nbsp;Aleksey V Lyundup","doi":"10.34172/bi.2021.22167","DOIUrl":"https://doi.org/10.34172/bi.2021.22167","url":null,"abstract":"<p><p><i><b>Introduction:</b></i> Mesenchymal stromal cells (MSCs) administration is an effective option for the treatment of diabetic foot ulcers (DFUs). However, to date, studies assessing long-term outcomes and evaluating skin parameters after cell-based therapy are lacking. We presented the clinical outcomes of 3 patients, treated for DFUs with the bone marrow MSCs 3 years earlier. <i><b>Methods:</b></i> Ultrasound examination was used to compare collagen density and epidermal thickness in areas of healed ulcers in comparison with non-affected skin used as a control. Ultrasound and dermatoscopy were used to exclude neoplasm formation, to assess scar contracture and wound recurrence. <i><b>Results:</b></i> In all patients, no ulcer recurrence was detected, which was lower than the expected 60% rate of re-ulceration in diabetic patients in a 3-year period (OD [odds ratio] = 0.095, <i>P</i> = 0.12). No neoplasm formation, no contracture of hypertrophic scar, and adjacent tissue were registered. Collagen ultrasound density was decreased by 57% (<i>P</i> = 0.053) and epidermal thickness was increased by 72% (<i>P</i> = 0.01) in the area of healed ulcers in all patients. <i><b>Conclusion:</b></i> MSCs therapy alone did not result in the complete restoration of the skin parameters within a 3-year period. MSCs may represent important adjuvant to the therapy, however, other novel approaches are required to achieve better results.</p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"51-55"},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/89/16/bi-12-51.PMC8783077.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39866853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the issue of transparency on the internal investigation of academic bullying.","authors":"Morteza Mahmoudi,&nbsp;Sherry Moss,&nbsp;Loraleigh Keashly","doi":"10.34172/bi.2021.44","DOIUrl":"https://doi.org/10.34172/bi.2021.44","url":null,"abstract":"<jats:p>\u0000 </jats:p>","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":" ","pages":"1-2"},"PeriodicalIF":2.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/37/b7/bi-12-1.PMC8783085.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39865405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}