Justine Daudi Maganira, Winifrida Kidima, Chacha John Mwita, Peter Halvarsson, Johan Höglund
{"title":"Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania.","authors":"Justine Daudi Maganira, Winifrida Kidima, Chacha John Mwita, Peter Halvarsson, Johan Höglund","doi":"10.1080/20008686.2020.1772668","DOIUrl":"https://doi.org/10.1080/20008686.2020.1772668","url":null,"abstract":"<p><p>The presence of<i>Taenia solium</i> DNA from eggs in soils around the households in four Tanzanian villages in Kongwa district were analysed in relation to seasonal fluctuations and infection risk implications. A total of 192 pooled soil samples from five sampling points per household were examined by droplet digital Polymerase Chain Reaction (ddPCR) from 96 pig-keeping households both during the dry and rainy seasons. The pooled samples were first processed by a flotation-double sieving technique, followed by screening for worm DNA employing universal primers targeting the mitochondrial cytochrome c oxidase subunit I (<i>cox1</i>) gene of human taeniid species and some other helminths. All DNA positive samples were later confirmed by a specific ddPCR probe assay targeting the mitochondrial <i>cox1</i> gene of <i>T. solium</i>. A total of 17.2% (n = 33) samples were positive with the universal ddPCR, whereas <i>T. solium</i> DNA was confirmed by the specific ddPCR only in 3.1% (n = 3) of the surveyed households. The detection of <i>T. solium</i> DNA in this study spells out a low risk of exposure to <i>T. solium</i> eggs from contaminated household soil. Based on our results, ddPCR seems to be a promising technology for screening <i>T. solium</i> eggs in soil.</p>","PeriodicalId":37446,"journal":{"name":"Infection Ecology and Epidemiology","volume":"10 1","pages":"1772668"},"PeriodicalIF":0.0,"publicationDate":"2020-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20008686.2020.1772668","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38373779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ram Pratim Deka, Ulf Magnusson, Delia Grace, Rajeswari Shome, Johanna F Lindahl
{"title":"Knowledge and practices of dairy farmers relating to brucellosis in urban, peri-urban and rural areas of Assam and Bihar, India.","authors":"Ram Pratim Deka, Ulf Magnusson, Delia Grace, Rajeswari Shome, Johanna F Lindahl","doi":"10.1080/20008686.2020.1769531","DOIUrl":"https://doi.org/10.1080/20008686.2020.1769531","url":null,"abstract":"<p><strong>Background: </strong>Brucellosis is one of the most common zoonotic diseases in the world. This study aimed at assessing farmers' knowledge about brucellosis as well as practices relevant to transmission of brucellosis and their associated determinants.</p><p><strong>Results: </strong>Few farmers knew about brucellosis (3.4%, <i>n</i> = 18) and its zoonotic importance (0.8%, <i>n</i> = 4). Knowledge about brucellosis was higher for farmers with a larger herd size (<i>p</i> < 0.001) and fully using a stall-fed system (<i>p</i> < 0.001). Training on dairy cattle management (<i>p</i> < 0.001), training on animal disease (<i>p</i> < 0.01), consultation with veterinarians (<i>p</i> < 0.001) and farms being in urban areas (<i>p</i> < 0.01) were also significantly positively associated with knowledge about brucellosis. No significant association was observed between farmers' knowledge about brucellosis and state, family size, education, age or gender of the farmers. Farmers knowledge about brucellosis was significantly associated with certain practices that include use of disinfectant while cleaning farms (<i>p</i> < 0.05), animal movement (<i>p</i> < 0.01), introduction of new animals (<i>p</i> < 0.05) and raw milk consumption (<i>p</i> < 0.05). The study did not find any association between knowledge about brucellosis and method of disposal of aborted materials, personal hygiene and quarantine practices.</p><p><strong>Conclusion: </strong>More interaction with veterinarians and training on animal management may be an important tool for generating awareness among the farming community for reducing transmission of the disease.</p>","PeriodicalId":37446,"journal":{"name":"Infection Ecology and Epidemiology","volume":"10 1","pages":"1769531"},"PeriodicalIF":0.0,"publicationDate":"2020-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20008686.2020.1769531","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38631201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohamed E El Zowalaty, Sean G Young, Josef D Järhult
{"title":"Environmental impact of the COVID-19 pandemic - a lesson for the future.","authors":"Mohamed E El Zowalaty, Sean G Young, Josef D Järhult","doi":"10.1080/20008686.2020.1768023","DOIUrl":"https://doi.org/10.1080/20008686.2020.1768023","url":null,"abstract":"<p><p>The environment is an integral component of human and animal health. COVID-19 is a global health challenge in the twenty-first century. The emergence of SARS-CoV-2 in Wuhan, China in December 2019, and its spread to regional countries and nowadays affecting more than 210 countries worldwide represents the first pandemic in history to be caused by a coronavirus. The COVID-19 pandemic has huge impacts on most aspects of human activities, as well as on the economy and health care systems. Lock-downs, quarantines and border closures in the wake of the pandemic have led to reductions in air pollution through decreased travel and production. These positive environmental effects are likely mostly temporary, but may serve as an example that changes in our way of life can have prompt positive effects for the environment and demonstrate the usefulness of travel-reducing measures such as teleconferencing. Thus, acknowledging that COVID-19 is first and foremost a global disaster, the pandemic may inspire to future behavioral changes with positive environmental effects.</p>","PeriodicalId":37446,"journal":{"name":"Infection Ecology and Epidemiology","volume":"10 1","pages":"1768023"},"PeriodicalIF":0.0,"publicationDate":"2020-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20008686.2020.1768023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38373778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tove Hoffman, Karolina Nissen, Janina Krambrich, Bengt Rönnberg, Dario Akaberi, Mouna Esmaeilzadeh, Erik Salaneck, Johanna Lindahl, Åke Lundkvist
{"title":"Evaluation of a COVID-19 IgM and IgG rapid test; an efficient tool for assessment of past exposure to SARS-CoV-2.","authors":"Tove Hoffman, Karolina Nissen, Janina Krambrich, Bengt Rönnberg, Dario Akaberi, Mouna Esmaeilzadeh, Erik Salaneck, Johanna Lindahl, Åke Lundkvist","doi":"10.1080/20008686.2020.1754538","DOIUrl":"https://doi.org/10.1080/20008686.2020.1754538","url":null,"abstract":"ABSTRACT COVID-19 is the most rapidly growing pandemic in modern time, and the need for serological testing is most urgent. Although the diagnostics of acute patients by RT-PCR is both efficient and specific, we are also crucially in need of serological tools for investigating antibody responses and assessing individual and potential herd immunity. We evaluated a commercially available test developed for rapid (within 15 minutes) detection of SARS-CoV-2-specific IgM and IgG by 29 PCR-confirmed COVID-19 cases and 124 negative controls. The results revealed a sensitivity of 69% and 93.1% for IgM and IgG, respectively, based solely on PCR-positivity due to the absence of a serological gold standard. The assay specificities were shown to be 100% for IgM and 99.2% for IgG. This indicates that the test is suitable for assessing previous virus exposure, although negative results may be unreliable during the first weeks after infection. More detailed studies on antibody responses during and post infection are urgently needed.","PeriodicalId":37446,"journal":{"name":"Infection Ecology and Epidemiology","volume":"10 1","pages":"1754538"},"PeriodicalIF":0.0,"publicationDate":"2020-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20008686.2020.1754538","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37895895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Do the current cases reported to the WHO provide a realistic incidence rate of countries infected with COVID-19?","authors":"Ghobad Moradi, Amjad Mohamadi Bolbanabad, Bakhtiar Piroozi, Ehsan Mostafavi, Arshad Veysi, Azad Shokri","doi":"10.1080/20008686.2020.1751917","DOIUrl":"https://doi.org/10.1080/20008686.2020.1751917","url":null,"abstract":"In compliance with the International Health Regulations (IHR), endorsed by 196 countries in 2005, it has been agreed that all World Health Organization (WHO) member states will contribute to preven...","PeriodicalId":37446,"journal":{"name":"Infection Ecology and Epidemiology","volume":"10 1","pages":"1751917"},"PeriodicalIF":0.0,"publicationDate":"2020-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20008686.2020.1751917","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37895893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tove Hoffman, Peter Wilhelmsson, Christos Barboutis, Thord Fransson, Thomas G T Jaenson, Per-Eric Lindgren, Friederike D Von Loewenich, Åke Lundkvist, Björn Olsen, Erik Salaneck
{"title":"A divergent <i>Anaplasma phagocytophilum</i> variant in an <i>Ixodes</i> tick from a migratory bird; Mediterranean basin.","authors":"Tove Hoffman, Peter Wilhelmsson, Christos Barboutis, Thord Fransson, Thomas G T Jaenson, Per-Eric Lindgren, Friederike D Von Loewenich, Åke Lundkvist, Björn Olsen, Erik Salaneck","doi":"10.1080/20008686.2020.1729653","DOIUrl":"https://doi.org/10.1080/20008686.2020.1729653","url":null,"abstract":"<p><p><i>Anaplasma phagocytophilum</i> (AP) has vast geographical and host ranges and causes disease in humans and domesticated animals. We investigated the role of northward migratory birds in the dispersal of tick-borne AP in the African-Western Palearctic. Ticks were collected from northward migratory birds trapped during spring migration of 2010 at two localities in the central Mediterranean Sea. AP DNA was detected by PCR (<i>gltA</i> and 16S rRNA) and variant determination was performed using <i>ankA</i> sequences. In total, 358 ticks were collected. One of 19 ticks determined as <i>Ixodes</i> was confirmed positive for AP DNA. The tick was collected from a woodchat shrike (<i>Lanius senator senator</i>) trapped in Greece, and molecularly determined to belong to the <i>I. ricinus</i> complex and sharing highest (95%) 16S RNA sequence identity to <i>I. gibbosus</i>. The <i>ankA</i> AP sequence exhibited highest similarity to sequences from rodents and shrews (82%) and ruminants (80%). Phylogenetic analyses placed it convincingly outside other clades, suggesting that it represents a novel AP variant. The divergent <i>Ixodes</i> species harboring a novel AP variant could either indicate an enzootic cycle involving co-evolution with birds, or dissemination from other regions by avian migration. None of the 331 <i>Hyalomma marginatum</i> sensu lato ticks, all immature stages, were positive for AP DNA, lending no evidence for the involvement of <i>Hyalomma</i> ticks transported by birds in the ecology of AP.</p>","PeriodicalId":37446,"journal":{"name":"Infection Ecology and Epidemiology","volume":"10 1","pages":"1729653"},"PeriodicalIF":0.0,"publicationDate":"2020-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20008686.2020.1729653","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37828983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sidhartha S Mohakud, Razibuddin Ahmed Hazarika, Sarat Sonowal, Durlav P Bora, Archana Talukdar, Santanu Tamuly, Johanna F Lindahl
{"title":"The extent and structure of pig rearing system in urban and peri-urban areas of Guwahati.","authors":"Sidhartha S Mohakud, Razibuddin Ahmed Hazarika, Sarat Sonowal, Durlav P Bora, Archana Talukdar, Santanu Tamuly, Johanna F Lindahl","doi":"10.1080/20008686.2020.1711576","DOIUrl":"https://doi.org/10.1080/20008686.2020.1711576","url":null,"abstract":"<p><p>Livestock is common in Indian cities and contribute to food security as well as livelihoods. Urban livestock keeping has been neglected, and in India, little is known about the topic. Therefore, urban and peri-urban pig farms of Guwahati, Assam, India, were surveyed in order to understand more about the pig rearing systems and risks of diseases. A total of 34 urban and 66 peri-urbanpig farms were selected randomly. All reared cross-bred pigs. Free-range pig rearing was common in both urban (58.8%) and peri-urban (45.45%) farms. Artificial insemination was used by around half of the pig farmers. Disinfection in pig farms was practiced in 26.5% of urban and 28.8% of peri-urban farms. More urban pig farms were observed to be moderately clean in (82.4%) compared to peri-urban (69.7%). However, more urban (67.7%) than peri-urban farms (57.6%) reported ahighrodent burden. Pig sheds were mostly basic, with bricked floors in 18.2% farms in peri-urban areas, and more than 80% had corrugated iron roofing sheets. In conclusion, free-roaming pigs in both urban and peri-urban areas of Guwahati can contribute to disease transmission, and the low standard of hygiene and buildings may further increase the risk of diseases.</p>","PeriodicalId":37446,"journal":{"name":"Infection Ecology and Epidemiology","volume":"10 1","pages":"1711576"},"PeriodicalIF":0.0,"publicationDate":"2020-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20008686.2020.1711576","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37597017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ayim-Akonor Matilda, May Juergen, Ralf Krumkamp, Harder Timm, Mertens Eva
{"title":"Molecular and serological prevalence of influenza A viruses in poultry and poultry farmers in the Ashanti region, Ghana.","authors":"Ayim-Akonor Matilda, May Juergen, Ralf Krumkamp, Harder Timm, Mertens Eva","doi":"10.1080/20008686.2019.1698904","DOIUrl":"https://doi.org/10.1080/20008686.2019.1698904","url":null,"abstract":"<p><p>For an analysis of the prevalence of influenza A viruses (IAVs) circulating in chickens and their farmers in the Ashanti region, Ghana, we examined 2,400 trachea and cloaca swabs (chickens) and 102 oropharyngeal swabs (farmers) by qRT-PCR. Sera from 1,200 (chickens) and 102 (farmers) were analysed for IAV antibodies by ELISA and haemagglutination inhibition (HI). Avian influenza virus (AIV) was detected in 0.2% (n = 5) of chickens but not farmers. Virus detection was more pronounced in the cloacal (n = 4, 0.3%) than in tracheal swabs (n = 1, 0.1%). AIV antibodies were not detected in chickens. Two farmers (2.0%) tested positive to human seasonal IAV H1N1pdm09. Sixteen (15.7%) farmers tested seropositive to IAV of which 68.8% (n = 11) were due to H1N1pdm09-specific antibodies. AIV H5- or H7-specific antibodies were not detected in the farmers. Questionnaire evaluation indicated the rare usage of basic personal protective equipment by farmers when handling poultry. In light of previous outbreaks of zoonotic AIV in poultry in Ghana the open human-animal interface raises concern from a OneHealth perspective and calls for continued targeted surveillance.</p>","PeriodicalId":37446,"journal":{"name":"Infection Ecology and Epidemiology","volume":"9 1","pages":"1698904"},"PeriodicalIF":0.0,"publicationDate":"2019-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20008686.2019.1698904","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37595972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jenny C Hesson, Emma Lundin, Åke Lundkvist, Jan O Lundström
{"title":"Surveillance of mosquito vectors in Southern Sweden for Flaviviruses and Sindbis virus.","authors":"Jenny C Hesson, Emma Lundin, Åke Lundkvist, Jan O Lundström","doi":"10.1080/20008686.2019.1698903","DOIUrl":"https://doi.org/10.1080/20008686.2019.1698903","url":null,"abstract":"<p><p>There are three human pathogenic bird-viruses transmitted by <i>Culex</i> mosquitoes in Europe: the alphavirus Sindbis and the flaviviruses West Nile virus and Usutu virus. Cases of Sindbis fever occur in the north while the flaviviruses are reported from southern Europe. In this study, 7933 <i>Culex pipiens/torrentium</i> mosquitoes from southern Sweden were screened by RTqPCR for these viruses. None of the mosquitoes were positive for viral RNA. The importance of mosquito species composition is discussed as a potential explanation to the lack of detection of mosquito-borne viruses in southern Sweden. However, continued surveillance of mosquitoes for Flaviviruses would be valuable as an early warning for public health awareness.</p>","PeriodicalId":37446,"journal":{"name":"Infection Ecology and Epidemiology","volume":"9 1","pages":"1698903"},"PeriodicalIF":0.0,"publicationDate":"2019-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/20008686.2019.1698903","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37470702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}