Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine最新文献_第8页

Bisalbuminemia: A Rare Incidental Finding in Monoclonal Gammopathy. 双白蛋白血症：单克隆丙种球蛋白病的罕见偶然发现

Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine Pub Date : 2023-12-21 eCollection Date: 2023-12-01

Sanjay Bagade, Arshiya Anjum

{"title":"Bisalbuminemia: A Rare Incidental Finding in Monoclonal Gammopathy.","authors":"Sanjay Bagade, Arshiya Anjum","doi":"","DOIUrl":"","url":null,"abstract":"Bisalbuminemia is a rare, benign, protein anomaly presenting with two distinct peaks of albumin on serum protein electrophoresis. It reflects the presence of a normal albumin and a modified albumin in the same individual. This condition can be either hereditary or acquired. Bisalbuminemias are more frequently encountered when serum protein electrophoresis is performed with capillary technique, because this offers better resolution compared to the conventional gel electrophoresis. There are very few case reports available in the literature, showing the presence of a bifid albumin peak along with a distinct paraprotein peak in the globulin region in serum protein electrophoresis. Here we are reporting two cases, a 46-year-old male and a 48 year-old male, diagnosed with multiple myeloma, revealing the presence of an extra peak in the albumin region along with a distinct paraprotein band, when the electrophoresis was performed using capillary mode. From these case reports, we wish to reveal the extremely rare nature of this entity and also to acquaint the clinicians and laboratory personnel with this pattern of electrophoretogram.","PeriodicalId":37192,"journal":{"name":"Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine","volume":"34 4","pages":"325-329"},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10828532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139673056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coffee Colored Serum, Adverse Reaction of Eltrombopag. 咖啡色血清，Eltrombopag的不良反应。

Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine Pub Date : 2023-10-16 eCollection Date: 2023-10-01

Carlos Rodríguez Rojas, Celia Juez Santamaría, Oscar David Pons Belda, Emilia Moreno Noguero

引用次数: 0

Evaluation of four quality indicators of the Pre-Analytical Phase External Quality Assessment Subprogram of the Fundación Bioquímica Argentina. 评估阿根廷生物基金会分析前阶段外部质量评估次级方案的四项质量指标。

Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine Pub Date : 2023-10-16 eCollection Date: 2023-10-01

Gisela Unger, Silvia Fabiana Benozzi, Raúl Girardi, Graciela Laura Pennacchiotti

{"title":"Evaluation of four quality indicators of the Pre-Analytical Phase External Quality Assessment Subprogram of the Fundación Bioquímica Argentina.","authors":"Gisela Unger, Silvia Fabiana Benozzi, Raúl Girardi, Graciela Laura Pennacchiotti","doi":"","DOIUrl":"","url":null,"abstract":"Pre-analytical phase external quality assessment programs contribute - through the interlaboratory comparison of quality indicators (QIs) - to the continuous improvement of the clinical laboratory total testing process. The purpose of the present work is to document the results derived from measuring four QIs within the framework of a pre-analytical phase external quality assessment subprogram in Argentina. The laboratories participating in this subprogram measured the following QIs: i) patients recalled for a new blood sample collection due to pre-analytical causes; ii) clotted samples from hemogram and coagulation tests; iii) clinical chemistry hemolyzed samples; and iv) requests with transcription errors entered into the laboratory information system. Results were expressed in percentage value and Sigma value. Databases were anonymized. A minimum acceptable quality level for the four QIs measured was recorded in the majority (75%) of the participating laboratories (Sigma > 3.0). It was nonetheless observed that the QIs of hemolyzed samples and requests with transcription errors entered into the laboratory information system deserve more attention. Through this pioneering experience in Argentina, the participating laboratories - some for the first time - could learn about their performance via interlaboratory comparison of results. This experience also proved to be motivating not only to improve the external assessment subprogram but also to continue working on the measurement of pre-analytical QIs for the continuous improvement of the clinical laboratory total testing process in Argentina.","PeriodicalId":37192,"journal":{"name":"Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine","volume":"34 3","pages":"203-212"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/56/a5/ejifcc-34-203.PMC10588077.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49692768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The parallel lives of prostate specific antigen in cardiac troponin assays. 心脏肌钙蛋白测定中前列腺特异性抗原的平行寿命。

Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine Pub Date : 2023-10-16 eCollection Date: 2023-10-01

Eleftherios P Diamandis

引用次数: 0

Disruption of laboratory activities during the COVID-19 pandemic: results of an EFLM Task Force Preparation of Labs for Emergencies (TF-PLE) survey. 新冠肺炎大流行期间实验室活动中断：EFLM应急实验室准备工作队（TF-PLE）调查结果。

Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine Pub Date : 2023-10-16 eCollection Date: 2023-10-01

Giuseppe Lippi, Janne Cadamuro, Elisa Danese, Emmanuel J Favaloro, Julien Favresse, Brandon M Henry, Snezana Jovicic, Tomris Ozben, Mario Plebani, Jecko Thachil

{"title":"Disruption of laboratory activities during the COVID-19 pandemic: results of an EFLM Task Force Preparation of Labs for Emergencies (TF-PLE) survey.","authors":"Giuseppe Lippi, Janne Cadamuro, Elisa Danese, Emmanuel J Favaloro, Julien Favresse, Brandon M Henry, Snezana Jovicic, Tomris Ozben, Mario Plebani, Jecko Thachil","doi":"","DOIUrl":"","url":null,"abstract":"Background: The EFLM Task Force Preparation of Labs for Emergencies (TF-PLE) created a survey that has been distributed to its members for gathering information on the key hazards experienced by European medical laboratories during the COVID-19 pandemic.Methods: The survey was distributed to over 12,000 potential contacts (laboratory workers) via an EFLM newsletter, with responses collected between May 8 and June 8, 2023.Results: Two hundred replies were collected and examined from European laboratories. 69.7% and 78.1% of all responders said they were short on non-COVID and COVID reagents, respectively. Exactly half of respondents (50.0%) said that they could not complete all laboratory tests required for a specific period, but this figure climbed to 61.2% for COVID tests. Finally, 72.3% of respondents expressed exhaustion during the pandemic, and 61.2% reported increasing patient hostility.Conclusions: The COVID-19 pandemic had a significant impact on laboratory medicine in Europe. Cultural change, proactive planning, and even re-engineering in some parts of the laboratory industry may thus be necessary to prepare for future challenges.","PeriodicalId":37192,"journal":{"name":"Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine","volume":"34 3","pages":"213-219"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/93/22/ejifcc-34-213.PMC10588075.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49692757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine Pub Date : 2023-10-16 eCollection Date: 2023-10-01

Lucio José Pantazis, Gustavo Daniel Frechtel, Gloria Edith Cerrone, Rafael García, Andrea Elena Iglesias Molli

{"title":"Phenotype similarities in automatically grouped T2D patients by variation-based clustering of IL-1β gene expression.","authors":"Lucio José Pantazis, Gustavo Daniel Frechtel, Gloria Edith Cerrone, Rafael García, Andrea Elena Iglesias Molli","doi":"","DOIUrl":"","url":null,"abstract":"Background: Analyzing longitudinal gene expression data is extremely challenging due to limited prior information, high dimensionality, and heterogeneity. Similar difficulties arise in research of multifactorial diseases such as Type 2 Diabetes. Clustering methods can be applied to automatically group similar observations. Common clinical values within the resulting groups suggest potential associations. However, applying traditional clustering methods to gene expression over time fails to capture variations in the response. Therefore, shape-based clustering could be applied to identify patient groups by gene expression variation in a large time metabolic compensatory intervention.Objectives: To search for clinical grouping patterns between subjects that showed similar structure in the variation of IL-1β gene expression over time.Methods: A new approach for shape-based clustering by IL-1β expression behavior was applied to a real longitudinal database of Type 2 Diabetes patients. In order to capture correctly variations in the response, we applied traditional clustering methods to slopes between measurements.Results: In this setting, the application of K-Medoids using the Manhattan distance yielded the best results for the corresponding database. Among the resulting groups, one of the clusters presented significant differences in many key clinical values regarding the metabolic syndrome in comparison to the rest of the data.Conclusions: The proposed method can be used to group patients according to variation patterns in gene expression (or other applications) and thus, provide clinical insights even when there is no previous knowledge on the subject clinical profile and few timepoints for each individual.","PeriodicalId":37192,"journal":{"name":"Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine","volume":"34 3","pages":"228-244"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cd/1b/ejifcc-34-228.PMC10588079.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49692771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Best Practices for Effective Management of Point of Care Testing. 有效管理护理点测试的最佳实践。

Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine Pub Date : 2023-10-16 eCollection Date: 2023-10-01

Anil K Chokkalla, Brandy D Recio, Sridevi Devaraj

{"title":"Best Practices for Effective Management of Point of Care Testing.","authors":"Anil K Chokkalla, Brandy D Recio, Sridevi Devaraj","doi":"","DOIUrl":"","url":null,"abstract":"With the recent COVID-19 pandemic, point-of-care testing has gained tremendous attention, particularly in acute care settings. The point-of-care testing landscape is rapidly expanding and being contemplated for any crucial test with a central laboratory turnaround time >25% of the clinical decision time. A typical point-of-care testing program within a large hospital system encompasses a multitude of operators utilizing a wide range of devices across multiple testing sites. Thus, managing a large point-of-care testing network remains a daunting task with challenges related to staffing, standardization, quality management, training and competency assessment, and data management. This review will focus on understanding the general organization as well as the roles and responsibilities of various point-of-care testing stakeholders in addressing these challenges. More importantly, it will discuss the strategies and best practices for effective point-of-care testing management based on consensus recommendations from professional societies as well as our experience at Texas Childrens Hospital.","PeriodicalId":37192,"journal":{"name":"Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine","volume":"34 3","pages":"245-249"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/56/5b/ejifcc-34-245.PMC10588082.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49692755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inter-laboratory method validation of CD34+ flow-cytometry assay: the experience of Turin Metropolitan Transplant Centre. CD34+流式细胞仪检测的实验室间方法验证：都灵大都会移植中心的经验。

Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine Pub Date : 2023-10-16 eCollection Date: 2023-10-01

I F Ivana Ferrero, D R Deborah Rustichelli, S C Sara Castiglia, L G Loretta Gammaitoni, A P Alessandra Polo, M P Marisa Pautasso, M G Massimo Geuna, F F Franca Fagioli

{"title":"Inter-laboratory method validation of CD34+ flow-cytometry assay: the experience of Turin Metropolitan Transplant Centre.","authors":"I F Ivana Ferrero, D R Deborah Rustichelli, S C Sara Castiglia, L G Loretta Gammaitoni, A P Alessandra Polo, M P Marisa Pautasso, M G Massimo Geuna, F F Franca Fagioli","doi":"","DOIUrl":"","url":null,"abstract":"The Turin Metropolitan Transplant Centre (CIC 305) includes four flow-cytometry laboratories assessing quality control on hematopoietic stem cells (HSC) with different instruments and operators. Therefore, the CD34+ enumeration assay should be validated on a regular basis. We describe here the validation plan to test the inter-laboratory reproducibility of CD34+ enumeration assay, based on the risk analysis. Stabilized blood samples were analysed using Stem-Kit reagent according to manufacturer's instructions and acquired using the Beckman Coulter Navios at Regina Margherita Children's' Hospital (305-1), Beckman Coulter FC500 at Candiolo Cancer Institute FPO-IRCCS (305-2), BD Biosciences FACSLyric™ at S. Luigi Hospital (305-3), and Beckman Coulter Navios EX at Mauriziano Hospital (305-4). The ISHAGE guidelines were followed for estimating % and absolute number of CD34+ cells in single-platform method. For each sample repeatability limit (r), reproducibility error, uncertainty of reproducibility error and coefficient of variation (CV) were reported. The repeated measurements from each laboratory or instrument have a variability, expressed as reproducibility error, lower than the repeatability limit for that single parameter. The corrected reproducibility error is always lower than the repeatability limit except for the percentage value of the \"low\" count. The analysis of inter-laboratory variance is within the maximum acceptable variance value, and the CV of all measurements for each parameter is less than 8%, indicating low measurement variability among laboratories. Evaluating the overall data, we can conclude that the four laboratories are perfectly aligned and the results are reproducible.","PeriodicalId":37192,"journal":{"name":"Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine","volume":"34 3","pages":"220-227"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/4e/58/ejifcc-34-220.PMC10588076.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49692769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monoclonal Light Chains with alpha 2 mobility on Serum Protein Electrophoresis. 血清蛋白电泳上具有α2迁移率的单克隆轻链。

Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine Pub Date : 2023-10-16 eCollection Date: 2023-10-01

S Danalakshmi

引用次数: 0

CA125, Galectin-3 and FGF-23 are interrelated in heart failure with reduced ejection fraction. CA125、Galectin-3 和 FGF-23 在射血分数降低的心力衰竭中相互关联。

Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine Pub Date : 2023-07-10 eCollection Date: 2023-07-01

Damien Gruson, Diane Maisin, Anne-Catherine Pouleur, Sylvie A Ann, Michel F Rousseau

{"title":"CA125, Galectin-3 and FGF-23 are interrelated in heart failure with reduced ejection fraction.","authors":"Damien Gruson, Diane Maisin, Anne-Catherine Pouleur, Sylvie A Ann, Michel F Rousseau","doi":"","DOIUrl":"","url":null,"abstract":"Background: Carbohydrate Antigen 125 (CA125) is the most widely used biomarker in ovarian cancer screening. In patients with heart failure (HF), increased levels of CA125 have been observed and related to disease severity. Our objective was to determine the association of CA125 levels with two biomarkers of adverse remodeling in HF patients with reduced ejection fraction (HFrEF).Methods: CA125 circulating levels were determined with an electrochemiluminscent immunoassay. Concentrations of B-type natriuretic peptide (BNP), N-terminal proBNP (Nt-proBNP), Galectin-3 and Fibroblast Growth Factor 23 (FGF23) were also measured by immunoassays.Results: CA125 levels were increased in HFrEF, were associated to disease severity according NYHA classes. Median CA125 concentration was also significantly related to cardiovascular mortality. CA125 concentrations were positively and significantly associated to Galectin-3 and FGF23.Conclusions: Concentrations of CA125 are increased in patients with HFrEF, associated to disease severity and adverse cardiovascular outcomes. CA125 levels are also correlated to Galectin-3 and FGF-23, two biomarkers related to fibrosis and cardiovascular remodeling.","PeriodicalId":37192,"journal":{"name":"Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine","volume":"34 2","pages":"103-109"},"PeriodicalIF":0.0,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c0/4c/ejifcc-34-103.PMC10349309.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10201125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0