BDJ Open最新文献

{"title":"Adipokines in oral squamous cell carcinoma-a narrative overview.","authors":"Pavithra Velusamy, Mary Mathew, Adarsh Kudva, Chetana Chandrashekar, Srikanth Gadicherla, Yoithaprabhunath Thuckanaickenpalayam Ragunathan, Sri Chinthu Kenniyan Kumar, Monica Charlotte Solomon","doi":"10.1038/s41405-026-00444-x","DOIUrl":"10.1038/s41405-026-00444-x","url":null,"abstract":"<p><strong>Objectives: </strong>To identify and summarize the types of adipokines associated with Oral Squamous Cell Carcinoma (OSCC) pathogenesis and prognosis.</p><p><strong>Materials and methods: </strong>A comprehensive literature search was done involving Scopus, PubMed, and Embase. Studies involving adipokines in OSCC conducted in humans were included.</p><p><strong>Results: </strong>Adipokines have diverse and stage-dependent effects on OSCC, including regulation of inflammation, angiogenesis, hypoxia, and tumour progression. Adipokines such as chemerin, resistin, leptin, and apelin contribute to tumour progression. Zinc alpha-2-glycoprotein and adiponectin were found to be antitumourigenic in OSCC. Methodological heterogeneity and a lack of standardized outcome measures limit comparability across studies to establish the definitive role of adipokines in OSCC.</p><p><strong>Conclusion: </strong>Adipokines shape OSCC biology through diverse, stage-specific mechanisms, making them potential biomarkers of disease progression. The adipokines reported in OSCC require validation through long-term prospective studies and addressing methodological heterogeneity to serve as prognostic markers.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":"12 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13176341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147943109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A national survey of foundation dentists' and educational supervisors' experiences: part 1 - motivations, achievements, and challenges.","authors":"Elizabeth Gonzalez Malaga, Sana Movahedi, Shalin Mehra, Vanessa Elaine Muirhead","doi":"10.1038/s41405-026-00433-0","DOIUrl":"10.1038/s41405-026-00433-0","url":null,"abstract":"<p><strong>Introduction: </strong>Completing Dental Foundation Training (DFT), guided by Educational Supervisors (ESs), is the route that almost every new UK dental graduate takes to work as independent NHS primary care performers. However, little is known about their achievements, motivations, and challenges. Few studies have included ESs.</p><p><strong>Aims: </strong>This study assessed reasons why Foundation Dentists (FDs) pursued DFT and the motivations of ESs. It examined their perceived achievements and challenges during DFT.</p><p><strong>Methods: </strong>An anonymous online survey was sent to FDs and ESs in England, Northern Ireland and Wales in February - April 2024 about their motivations, achievements, and challenges.</p><p><strong>Results: </strong>469 FDs (52% response rate) and 640 (71% response rate) ESs participated. Most (91%) FDs wanted to build their clinical confidence, and ESs (98%) wanted to explore career opportunities and share knowledge (94%). While 96% of FDs felt that DFT had enhanced their clinical skills, 62% faced challenges providing complex care. ESs (37%) had difficulty managing trainees' performances. Six percent and 4% of FDs had experienced bullying and harassment from patients, and ESs or the dental team respectively.</p><p><strong>Conclusions: </strong>DFT provided opportunities for new graduates to develop clinical skills and confidence. Actions should address challenges in DFT related to providing complex clinical care and bullying and harassment.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":"12 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13168312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147934245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the prospects of artificial intelligence in transforming dental care for special needs groups: mapping the current evidence.","authors":"Mithun Pai, Shweta Yellapurkar, Kavery Chengappa S, Kalyana C Pentapati","doi":"10.1038/s41405-026-00436-x","DOIUrl":"10.1038/s41405-026-00436-x","url":null,"abstract":"<p><strong>Background: </strong>The integration of Artificial Intelligence (AI) in providing quality dental care to individuals with special needs has been scarcely explored and holds the potential to be transformative. This study aimed to map the current evidence and research gaps on the application of AI tools in special care dentistry.</p><p><strong>Methodology: </strong>This study was conducted in accordance with the Preferred Reporting Items for Systematic reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) guidelines to ensure transparency. A systematic search was carried out across PubMed, Google Scholar, Scopus and Web of Science, including studies on AI for dental care of special needs groups published from 2015 to 2025. The data were charted on an evidence map, and study characteristics were evaluated to identify existing evidence and gaps.</p><p><strong>Results: </strong>Five studies were included in the review. Some of the major themes explored were AI tools used for diagnosis, treatment planning, behavior management, remote consultation and communication assistance for these groups, with one of the five studies assessing dentists' perceptions of their use. This study highlighted the lack of robust evidence and the narrow focus of the existing studies.</p><p><strong>Conclusions: </strong>This study highlighted the research gap in AI tools for special needs groups and noted the dearth of scientifically and conceptually rigorous studies on the topic. Thus, serving as the preliminary evidence for directing subsequent research on clinical translation of AI tools for special needs groups.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":"12 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13157484/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147864595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remineralization of early caries lesions by calcium hypophosphite in vitro: a surface microhardness study.","authors":"Bennett Tochukwu Amaechi, Razina Vohra, Sima Abdollahi, Kelly Yang, Amos Chinedu Obiefuna, Erik Schulze Zur Wiesche, Joachim Enax","doi":"10.1038/s41405-026-00440-1","DOIUrl":"10.1038/s41405-026-00440-1","url":null,"abstract":"<p><strong>Aim: </strong>This in vitro study used a pH cycling model to compare the caries remineralizing efficacy of toothpaste formulations containing calcium hypophosphite (CaP), hydroxyapatite (HAP), or sodium fluoride (NaF).</p><p><strong>Methods: </strong>Bovine enamel blocks with plaque-induced initial caries lesions were randomized (n = 30/group) to toothpaste formulations containing 1% CaP, 20% HAP, 1% CaP+20% HAP, or 1450 ppm fluoride provided as NaF. Lesion-bearing samples were subjected to a 14-day remineralization using a pH-cycling model with daily regimen of three 2-min applications of toothpaste slurry (1:3 toothpaste: water), one 2-h acid exposure, and storage in artificial saliva for the rest of the day. Remineralization was quantified as change in surface microhardness (SMH) of each sample measured before and after toothpaste treatment and expressed as percent remineralization (%Rem). Statistical analyses included paired t tests for within-group changes and one-way ANOVA with Tukey's post hoc tests for between-group comparisons (α = 0.05).</p><p><strong>Results: </strong>All groups exhibited significant (paired t test, p < 0.001) increase in SMH from baseline, indicating remineralization. Combining CaP and HAP achieved significantly (ANOVA/Tukey's, p < 0.001) greater %Rem (89.7 ± 3.3) when compared with CaP alone (75.4 ± 5.5), HAP alone (62.4 ± 4.8), or NaF alone (60.3 ± 7.8). While %Rem achieved with CaP alone was significantly (p < 0.001) greater than that of HAP and NaF, there was no significant difference in %Rem between HAP and NaF.</p><p><strong>Conclusion: </strong>This in vitro study demonstrates that CaP alone, and in combination with HAP, increases enamel surface microhardness, confirming that CaP-based formulations are efficient strategies for the remineralization of early caries lesions.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":"12 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13153252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of digital splints versus traditional splints for bruxism management: a systematic review.","authors":"Ravinder S Saini, Kanwalpreet Kaur, Seyed Ali Mosaddad, Artak Heboyan","doi":"10.1038/s41405-026-00438-9","DOIUrl":"10.1038/s41405-026-00438-9","url":null,"abstract":"<p><strong>Background: </strong>The prevalence of bruxism in the adult population varies widely. Splints are commonly used to treat bruxism, protect teeth, and alleviate the adverse effects of grinding or clenching. This systematic review aimed to compare the effectiveness of digital versus traditional splints in managing bruxism, focusing in clinical outcomes such as symptom severity, pain reduction, bruxism event frequency, and muscle activity.</p><p><strong>Methods and material: </strong>A Systematic review was conducted following PRISMA guidelines. A comprehensive electronic database search was conducted across PubMed, Scopus, the Cochrane Library, Dimensions, and Google Scholar for scholarly journal articles comparing digital and traditional splints. Eligibility criteria included randomized controlled trials (RCTs) published in peer-reviewed journals that investigated the effectiveness of splints in bruxism management. The Cochrane Risk of Bias 2.0 was used to appraise the quality of the included randomized controlled trials.</p><p><strong>Results: </strong>The literature search yielded 2115 records, of which eight articles met the eligibility criteria. Additionally, the risk of bias assessment results indicated that most randomized controlled trials reported a low to moderate risk of bias. This study's findings demonstrate the superiority of digital splints over traditional splints in managing bruxism. However, this difference was not statistically significant.</p><p><strong>Conclusions: </strong>Digital splints showed a tendency toward improved outcomes in managing bruxism compared to traditional splints, but the lack of statistical significance highlights the need for further research to confirm these findings.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":"12 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13156276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147857387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative effectiveness of alloplastic and biologic grafts in maxillary sinus augmentation: a systematic review.","authors":"Jonathan Varghese Thomas, Santosh Martande, Joshua Thomas Meenathathil, Bhushan Bhagat, Swathi Pv, Shambhavi Thakur, Krishna Suryawanshi, Nomita Yein","doi":"10.1038/s41405-026-00435-y","DOIUrl":"10.1038/s41405-026-00435-y","url":null,"abstract":"<p><strong>Background: </strong>Maxillary sinus augmentation is a well-established procedure to increase posterior maxillary bone volume for implant placement. Various graft materials are used, including autografts, allografts, xenografts, and alloplasts, but the clinical performance of alloplasts remains debated.</p><p><strong>Methodology: </strong>A systematic review was performed following PRISMA guidelines to compare histological, histomorphometric, radiographic, and clinical outcomes of sinus lift procedures using different grafts. Electronic searches were conducted in eight databases. Eligible studies were human clinical trials (RCTs, cohorts) comparing at least two graft materials and reporting new bone formation, graft resorption, implant survival, or complications. Eighteen studies met the inclusion criteria. Risk of bias was assessed using Cochrane RoB 2.0 and ROBINS I V2 tool.</p><p><strong>Results: </strong>Alloplastic grafts demonstrated comparatively lower new bone formation than autogenous and allogeneic grafts, and were generally inferior to xenografts in terms of regenerative potential. They exhibited higher residual graft content, suggesting slower resorption when compared with biologic grafts. In contrast, xenografts and allografts showed more favorable integration. Volumetric outcomes indicated that alloplastic grafts provided better dimensional stability than autografts but were less effective than xenografts in maintaining graft volume and space. Radiographic findings further supported superior space maintenance with xenografts compared to alloplastic grafts. Implant survival rates were high across all graft types; however, biologic grafts demonstrated relatively improved success and lower marginal bone loss compared with alloplastic grafts.</p><p><strong>Conclusion: </strong>Autografts demonstrated the highest effectiveness in maxillary sinus augmentation, followed by xenografts and alloplastic grafts, indicating a comparatively lower regenerative potential of alloplastic materials. Nevertheless, alloplastic grafts may still be considered a viable alternative in selected clinical scenarios, particularly where minimizing donor-site morbidity or reducing immunological and infection-related risks is a priority. High-quality, long-term randomized trials are needed to confirm these findings and optimize graft selection.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":"12 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13153208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147844019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alkaline phosphatase activity in gingival crevicular fluid during orthodontic treatment with different extraction protocols for maxillary canines: a randomized controlled trial.","authors":"Yahya Dakdouk, Kindah Sultan, Shadi Azzawi","doi":"10.1038/s41405-026-00425-0","DOIUrl":"10.1038/s41405-026-00425-0","url":null,"abstract":"<p><strong>Background: </strong>Alkaline phosphatase (ALP) activity in gingival crevicular fluid (GCF) reflects localized periodontal and alveolar bone remodeling during orthodontic tooth movement. Although ALP changes have been investigated during leveling and alignment or active orthodontic movement, the biological impact of tooth extraction timing relative to orthodontic force application remains insufficiently characterized.</p><p><strong>Objective: </strong>This randomized controlled trial aimed to compare ALP activity in gingival crevicular fluid between different extraction protocols in maxillary canines over a 6-week period. The primary outcome was the between-group difference in ALP activity across repeated time points.</p><p><strong>Methods: </strong>Sixty patients with moderate maxillary crowding were randomly allocated, using concealed allocation into three equal groups: Group A (leveling and alignment only with delayed extraction), Group B (extraction only), and Group C (simultaneous extraction with leveling and alignment). GCF samples were collected weekly from Mesial and Distal sites of maxillary canines from baseline to week six. ALP activity was quantified using a spectrophotometric method. Data distribution was assessed prior to analysis. Due to predominantly non-normal distributions, non-parametric tests were primarily applied, including Friedman's test for intragroup comparisons and Kruskal-Wallis test for intergroup comparisons, with appropriate post-hoc analyses.</p><p><strong>Results: </strong>Distinct temporal patterns of ALP activity were observed across the three groups. Group B (extraction only) demonstrated the highest ALP activity during the early time points, particularly at weeks 1-3, while Group C showed intermediate responses and Group A exhibited consistently lower levels. At T1, significant intergroup differences were observed. At Mesial sites, Group C showed higher ALP activity than Group A (mean difference: 0.59 IU/L), followed by Group B (mean difference: 0.28 IU/L compared with Group A; p < 0.05). At Distal sites, Group B demonstrated the highest ALP activity compared to Group A (mean difference: 1.00 IU/L; p < 0.01), with Group C showing intermediate values compared with Group A (mean difference: 0.73 IU/L).</p><p><strong>Conclusions: </strong>Tooth extraction was associated with increased ALP activity in gingival crevicular fluid during the early phase of orthodontic treatment. Variations in ALP patterns between extraction protocols indicate that extraction timing modulates early biochemical responses in periodontal tissues.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":"12 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135509/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence factors of early childhood caries risk among children aged 1-2 years in Beijing: a prospective cohort study.","authors":"Shuxi Miao, Mei Zhao, Wen Ren, Wei Chen, Hui Zhang, Min Liu","doi":"10.1038/s41405-026-00432-1","DOIUrl":"10.1038/s41405-026-00432-1","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate risk factors for early childhood caries (ECC) among 1-2-year-old children in Beijing and to assess the effect modification of baseline caries experience on the associations between other risk factors and subsequent caries outcomes.</p><p><strong>Methods: </strong>A 12-24-month prospective study was conducted from 2021 to 2023, a total of 919 participants with valid data were included in this study, with a follow-up rate of 76.9%. Oral health information and related factors were collected through parent-completed questionnaires and clinical dental examinations. Univariate analyses and a zero-inflated negative binomial (ZINB) regression model were used to identify risk factors.</p><p><strong>Results: </strong>Caries incidence during follow-up was 29.8%, and the mean increase in decayed, missing, and filled primary teeth (Δdmft) was 0.94 ± 1.94. In the full-sample ZINB model, significant predictors of caries risk were frequency of snack intake, frequency of candy consumption, frequency of bedtime tooth brushing, frequency of bottle use at bedtime, and baseline caries status. In the baseline caries-free group, additional significant predictors of incident caries risk were parents' caries status, frequency of saliva-sharing behavior, frequency of post-meal mouth rinsing, and the presence of additives in daily drinking water. Interaction analyses showed that the effects of parents' caries status, saliva-sharing frequency, post-meal mouth rinsing frequency, presence of additives in daily drinking water, bedtime brushing frequency, and history of dental attendance on caries risk differed significantly between children with and without baseline caries.</p><p><strong>Conclusion: </strong>The risk of early childhood caries (ECC) among 1-2-year-old children in Beijing was associated with multiple oral health-related factors. Baseline caries experience was a strong predictor of subsequent caries risk and significantly modified the effects of other risk factors.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":"12 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OSRA^QMUL: a digital application for oral surgery risk assessment.","authors":"Haidar Hassan, Hussein Al-Tamimi, Rawand Shado, Saffa Dean, Ravi Rathod, Paulo Oliva, Atif Matin","doi":"10.1038/s41405-026-00437-w","DOIUrl":"10.1038/s41405-026-00437-w","url":null,"abstract":"<p><strong>Background: </strong>Adherence to clinical guidelines is central to patient safety in oral surgery, yet guideline uptake in routine dental practice remains inconsistent. Traditional guideline formats, such as static PDF documents, can be difficult to navigate at the point of care. Digital decision-support tools may improve accessibility, efficiency and clinician confidence, but evidence specific to oral surgery is limited.</p><p><strong>Aim: </strong>To evaluate whether the Oral Surgery Risk Assessment (OSRA) digital application improves usability, efficiency, perceived clinical impact and user satisfaction compared with conventional PDF-based guideline access.</p><p><strong>Methods: </strong>A randomised crossover study was conducted among 30 participants from a single academic centre, including undergraduate dental students, junior dental surgeons (DFTs/DCTs) and senior clinicians. Participants used both the OSRA digital application and conventional PDF guidelines across standardised oral surgery clinical scenarios. Outcomes were assessed using a structured questionnaire covering usability, reliability and trust, efficiency and workflow, satisfaction and future use and perceived clinical impact, measured on five-point Likert scales. Paired comparisons were analysed using Wilcoxon signed-rank tests, with effect sizes reported. Qualitative free-text feedback was analysed thematically.</p><p><strong>Results: </strong>Across all domains, the OSRA application was consistently rated higher than PDF guidelines (all p < 0.05), with large to very large effect sizes (r = 0.55-0.75). The greatest differences were observed for efficiency and workflow (r = 0.75) and satisfaction and future use (r = 0.73). Participants reported reduced cognitive load, faster access to guidance and greater confidence in clinical decision making when using the app. Reliability analysis demonstrated excellent internal consistency for app-based ratings (Cronbach's α = 0.96). Qualitative feedback highlighted clarity, intuitive navigation and workflow integration as key strengths, alongside requests for enhanced transparency of evidence sources and expanded functionality.</p><p><strong>Conclusions: </strong>In this pilot study, the OSRA digital application outperformed conventional PDF guidelines across all evaluated domains, suggesting that digital, point of care decision support tools can improve guideline accessibility, efficiency and user experience in oral surgery. With further development and validation, such tools have the potential to support safer, more consistent evidence-based clinical practice.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":"12 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13133352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A preliminary evaluation of quercetin-mediated osteogenic gene expression in lipopolysaccharide-treated human periodontal ligament cells: an in vitro study.","authors":"Srishta Radhakrishnan, Prem Blaisie Rajula M, P L Ravi Shankar, Merita S, V Kalaivani, Rahila C","doi":"10.1038/s41405-026-00434-z","DOIUrl":"10.1038/s41405-026-00434-z","url":null,"abstract":"<p><strong>Aim: </strong>This study aimed to evaluate the effect of quercetin on osteogenic gene expression in lipopolysaccharide (LPS) stimulated human periodontal ligament cells (hPDLCs) under in vitro conditions.</p><p><strong>Materials and methods: </strong>hPDLCs were cultured with LPS for 24 hours to simulate an inflammatory microenvironment. Following this pre-stimulation, cells were treated with quercetin at concentrations of 2.5 µM, 5 µM, and 10 µM for up to 14 days. No further exposure to LPS was performed during the subsequent culture period. The mRNA expression levels of osteogenic markers, osteopontin (OPN) and osteocalcin (OCN), were assessed on day 14 using Quantitative Real-Time PCR (RT-qPCR). Statistical analysis was performed using Welch's one-way ANOVA with Holm-adjusted post hoc comparisons.</p><p><strong>Results: </strong>LPS stimulation significantly suppressed the expression of both OPN and OCN compared with the control group. However, quercetin treatment restored and dose-dependently increased the expression of these markers, with the greatest effect observed at 10 µM. At this concentration, OPN and OCN expression levels reached 5.80 ± 0.26 and 6.62 ± 0.30, respectively, relative to the control. A consistent dose-dependent upregulation was observed for both markers, indicating restoration of osteogenic gene expression.</p><p><strong>Conclusion: </strong>Quercetin modulates the expression of osteogenic markers, including OPN and OCN, in LPS-stimulated hPDLCs under in vitro conditions. These findings suggest a potential modulatory role for quercetin in influencing osteogenic gene expression. However, additional in vitro functional assays and in vivo studies are necessary to establish its role in periodontal regeneration.</p>","PeriodicalId":36997,"journal":{"name":"BDJ Open","volume":"12 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13121475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147783172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}