Antibacterial and Cytotoxicity characteristics of experimental epoxy -based endodontic sealer loaded with silver gold nanoparticles: in vitro study.

IF 2.5 Q2 DENTISTRY, ORAL SURGERY & MEDICINE
Nermine Hassan, Mona Riad, Shereen Hafez Ibrahim, Khaled Mahmoud, Bassam Ahmed Abulnoor, Reham Hassan
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Abstract

Background: Enhancing the antibacterial capabilities of dental materials by adding nanoparticles has been the subject of some research. However, the potential toxic effect of this material on the vital tissues should be investigated to avoid additional damage to the tissue.

Objective: This study aimed to validate the long-term cytotoxic and antibacterial properties of an epoxy resin-based endodontic sealer (AH Plus) with and without loading with silver gold nanoparticles (Nano Care Plus Silver Gold®).

Material and methods: The tested groups were Nano Care Gold (group I), modified resin sealer (m AH Plus; group II) and AH Plus served as a control group (group III). Agar diffusion was used to measure the antibacterial activity against Enterococcus faecalis. Using the MTT test, cytotoxicity assessment was carried out in accordance with ISO-10993-5 guidelines to assess the cells' viability as soon as possible and after two and four weeks. The t-test was used to statistically examine the data. The chosen significance threshold was P <0.05.

Results: Antibacterial results revealed that there was no difference in the diameter of the inhibition zones measured in all groups at 24 h. While in 48 and 72 h, the difference was statistically significant (p <0.05). In 48-h Nanogold was significantly higher than AH Plus when tested alone (p <0.05), however their mixture showed insignificant difference. After 72 h, the Nano gold was significantly higher than that of AH Plus & Nano gold mixture (p <0.05). Cytotoxicity result revealed there was a significant difference between tested groups at different intervals (p <0.001). For immediate measurements, values measured with the AH group were significantly higher than those of other groups (p <0.001). For the AH& nanogold group, there was no significant difference between values measured at different intervals (p = 0.578).

Conclusions: Silver gold nanoparticles have acceptable antibacterial properties and low cytotoxicity to be used as canal pretreatment prior to the application of the sealer or even incorporated with AH Plus sealer.

含银金纳米粒子的实验性环氧树脂牙髓封闭剂的抗菌和细胞毒性特性:体外研究。
背景:通过添加纳米粒子增强牙科材料的抗菌能力一直是一些研究的主题。然而,应研究这种材料对重要组织的潜在毒性作用,以避免对组织造成额外损害:本研究旨在验证添加和未添加纳米银金颗粒(Nano Care Plus Silver Gold®)的环氧树脂基牙髓封闭剂(AH Plus)的长期细胞毒性和抗菌特性:测试组为 Nano Care Gold(I 组)、改性树脂封闭剂(m AH Plus;II 组)和作为对照组的 AH Plus(III 组)。采用琼脂扩散法测定对粪肠球菌的抗菌活性。根据 ISO-10993-5 指南,使用 MTT 试验进行细胞毒性评估,以尽快评估细胞的存活率,并在两周和四周后进行评估。采用 t 检验对数据进行统计分析。选择的显著性临界值为 P 结果:抗菌结果显示,在 24 小时内,各组测定的抑菌区直径没有差异,而在 48 小时和 72 小时内,差异有统计学意义(P 结论:银金纳米粒子具有可接受的抗菌效果:银金纳米粒子具有可接受的抗菌性能和低细胞毒性,可在使用封闭剂前用作牙道预处理,甚至与 AH Plus 封闭剂混合使用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BDJ Open
BDJ Open Dentistry-Dentistry (all)
CiteScore
3.70
自引率
3.30%
发文量
34
审稿时长
30 weeks
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