Nanotheranostics最新文献_第4页

Microemulsion Gels of Tapentadol Hydrochloride: Statistical Analysis of Pharmacokinetics and Skin Irritation Studies. 盐酸他他多微乳凝胶：药代动力学统计分析及皮肤刺激研究。

Nanotheranostics Pub Date : 2025-05-07 eCollection Date: 2025-01-01 DOI: 10.7150/ntno.110819

Nimmathota Madhavi, Naveen Kumar Ganji, Heera Battu, Beeravelli Sudhakar

{"title":"Microemulsion Gels of Tapentadol Hydrochloride: Statistical Analysis of Pharmacokinetics and Skin Irritation Studies.","authors":"Nimmathota Madhavi, Naveen Kumar Ganji, Heera Battu, Beeravelli Sudhakar","doi":"10.7150/ntno.110819","DOIUrl":"10.7150/ntno.110819","url":null,"abstract":"Background: The present study aimed to overcome the drawbacks of tapentadol through the oral route and to assess the significance of microemulsion gels for transdermal delivery via pharmacokinetic approach. Methods: Microemulsions were prepared via a ternary phase diagram. The optimized microemulsion was converted into gels, and the microemulsion was evaluated for particle size, zeta potential and cumulative in vitro drug release, whereas the gel was characterized for viscosity, spreadability, in vitro, ex vivo, in vivo and skin irritation studies. The prepared ME-gel PKs were tested against MEs, oral solution and plain gel. Results: The PK study revealed that the half-life of the ME gel was 2.2-fold greater than that of the oral solution and 1.65-fold greater than that of the plain gel. The MRT of the ME gel was 6-fold greater than that of the oral solution and 3.3-fold greater than that of the plain gel. The overall mean value of the AUC (0-∞) was 3.16 times greater than that of the oral route. The skin irritation studies found that absence of irritation and damage after application of ME-gel. Conclusion: The PK study revealed that ME-gel was effective in pain management. The level A IVIVC between the in vitro fraction of drug released and the fraction of drug absorbed in vivo was 0.9731.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"9 2","pages":"144-154"},"PeriodicalIF":0.0,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12188534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the potential of Urolithin A in Cancer Therapy: Mechanistic Insights to Future Perspectives of Nanomedicine. 揭示尿素A在癌症治疗中的潜力：纳米医学未来前景的机制见解。

Nanotheranostics Pub Date : 2025-04-22 eCollection Date: 2025-01-01 DOI: 10.7150/ntno.110966

Vinita Karumuru, Anupam Dhasmana, Narsimha Mamidi, Subhash C Chauhan, Murali M Yallapu

{"title":"Unveiling the potential of Urolithin A in Cancer Therapy: Mechanistic Insights to Future Perspectives of Nanomedicine.","authors":"Vinita Karumuru, Anupam Dhasmana, Narsimha Mamidi, Subhash C Chauhan, Murali M Yallapu","doi":"10.7150/ntno.110966","DOIUrl":"10.7150/ntno.110966","url":null,"abstract":"Cancer remains a leading global health challenge, with high mortality rates persisting despite significant advancements in therapeutic interventions. Major obstacles, including systemic toxicity, therapy resistance, metastasis, and relapses, emphasize the urgent need for safer, more effective, and readily accessible treatment strategies. Among emerging alternatives, natural bioactive compounds have gained considerable attention because of their diverse therapeutic potential and lower toxicity profiles. Urolithin A (UA), a metabolite derived from ellagic acid through gut microbiota metabolism, has emerged as a compelling anticancer agent. UA has multiple mechanisms of action, including the regulation of autophagy, enhancement of mitochondrial function, and inhibition of tumor progression and metastatic pathways. Additionally, its chemo-, immuno-, and radio-sensitization properties further increase its therapeutic advantages. Nanotechnology-driven approaches, such as nanoparticle formulations, lipids, and powder formulations, have successfully increased the solubility, stability, bioavailability, precise targeted delivery to cancer tissues, and overall therapeutic benefits of these materials. This review comprehensively explores the anticancer mechanisms of UA, its modulatory role, and advances in nanoformulation strategies, highlighting its potential as a next-generation therapeutic agent for improved cancer treatment and prevention.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"9 2","pages":"121-143"},"PeriodicalIF":0.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12188533/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144498270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomics for the Identification of Biomarkers in Kidney Diseases. 代谢组学在肾脏疾病生物标志物鉴定中的应用。

Nanotheranostics Pub Date : 2025-03-24 eCollection Date: 2025-01-01 DOI: 10.7150/ntno.108320

Swarnima Pandey

引用次数: 0

GMP manufacturing of umbilical cord blood mesenchymal stem cell-released exosomes and verification of wound healing efficacy. 脐血间充质干细胞释放外泌体的GMP制造及创面愈合效果的验证。

Nanotheranostics Pub Date : 2025-03-03 eCollection Date: 2025-01-01 DOI: 10.7150/ntno.105433

Jae-Yong Lee, Jaehyoung Kim, Kyuheum Na, Hee-Jin Ahn

{"title":"GMP manufacturing of umbilical cord blood mesenchymal stem cell-released exosomes and verification of wound healing efficacy.","authors":"Jae-Yong Lee, Jaehyoung Kim, Kyuheum Na, Hee-Jin Ahn","doi":"10.7150/ntno.105433","DOIUrl":"10.7150/ntno.105433","url":null,"abstract":"Rationale: Drug development research using exosomes is being actively conducted worldwide. However, clear management standards and verification systems for the use of exosomes as drugs are still being developed. In this study, the effectiveness of exosomes as drug candidates was verified after production in accordance with the guidelines for donor suitability, cell bank construction of cell therapy drugs, and quality control of extracellular vesicles published by the Korean Ministry of Food and Drug Safety (KMFDS). Methods: Basic characteristics were identified using umbilical cord blood mesenchymal stem cells (UCBMSCs)-released exosomes produced in accordance with the three guidelines, and internal component analysis of proteins, lipids, and nucleic acids was performed. In addition, various types of in vitro and in vivo experiments confirmed the skin tissue regeneration efficacy of exosomes. Results: In addition, treatment of two types of skin cells (dermal fibroblast and keratinocyte) with exosomes resulted in a statistically significant increase in the proliferation and migration of skin cells and inhibition of the secretion of pro-inflammatory substances in an inflammatory environment. In an animal model of wound injury, exosome treatment accelerated the wound healing process. These in vitro and in vivo experiments confirm that UCBMSCs-released exosomes have tissue regeneration and inflammation suppression properties. Conclusions: This study presents the processes and quality control items that can produce exosomes as drugs in good manufacturing practice facilities and shows the possibility of developing drugs for various diseases based on the inherent efficacy of UCBMSCs-released exosomes. This study is the first to show how exosomes can be produced and quality-validated in a GMP facility for use as drugs, which will accelerate the time to market for future exosomes base bio-new drug.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"9 2","pages":"95-109"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11980038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A miniature low-immunogenic platform for the biosynthesis of self-assembling protein nanoparticles. 用于自组装蛋白质纳米颗粒生物合成的微型低免疫原性平台。

Nanotheranostics Pub Date : 2025-03-03 eCollection Date: 2025-01-01 DOI: 10.7150/ntno.98946

Almina I Polinova, Anna V Serkina, Marina V Volkova, Aleksandr A Gorbunov, Evgeniia P Sannikova, Irek I Gubaidullin, Aleksandr S Komolov, Anna V Rybakova, Marina Yu Kopaeva, Konstantin S Plokhikh, Georgy S Peters, Artem A Shatilov, Alexander A Shtil, Galina A Posypanova, Alexander P Trashkov, Natalia V Bulushova, Dmitry G Kozlov

{"title":"A miniature low-immunogenic platform for the biosynthesis of self-assembling protein nanoparticles.","authors":"Almina I Polinova, Anna V Serkina, Marina V Volkova, Aleksandr A Gorbunov, Evgeniia P Sannikova, Irek I Gubaidullin, Aleksandr S Komolov, Anna V Rybakova, Marina Yu Kopaeva, Konstantin S Plokhikh, Georgy S Peters, Artem A Shatilov, Alexander A Shtil, Galina A Posypanova, Alexander P Trashkov, Natalia V Bulushova, Dmitry G Kozlov","doi":"10.7150/ntno.98946","DOIUrl":"10.7150/ntno.98946","url":null,"abstract":"Aims: Previously, to obtain antigen-presenting self-assembling protein nanoparticles (SAPN), we developed a biosynthetic platform combining the self-associating peptide L6KD and the SUMO protein. In the current work, the immunogenic SUMO was replaced with an artificial 30 amino acid long peptide pepA1. Methods: The immunogenic properties of the pepA1-SAPN were tested in mice using the pneumococcal PhtD19 and ovalbumin OVA257-280 antigens in the absence of adjuvants. Results and Conclusions: The updated SAPN showed a 100% seroconversion rate and low immunogenicity of the platform. Given the effective synthesis and improved purification procedure, the pepA1-based miniature platform looks promising for development of vaccines and vehicles for targeted delivery.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"9 1","pages":"67-81"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11898719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Potential of Cetuximab Conjugated Hydroxyapatite Zirconium Nanoparticle as Nanocarrier for Radioenhancer in X-Ray Dynamic Therapy and ¹⁷⁷Lu-based Radioimmunotherapy of Lung Cancer. 西妥昔单抗结合羟基磷灰石锆纳米颗粒作为放射增强剂纳米载体在肺癌x射线动态治疗和177lu放射免疫治疗中的双重潜力。

Nanotheranostics Pub Date : 2025-03-03 eCollection Date: 2025-01-01 DOI: 10.7150/ntno.101699

Ahmad Kurniawan, Isa Mahendra, Asep Rizaludin, Marhendra Satria Utama, Ronny Lesmana, Fitria Dwi Ayuningtyas, Maria Rosa Dewanti, Dani Gustaman Syarif, Muhamad Basit Febrian

{"title":"Dual Potential of Cetuximab Conjugated Hydroxyapatite Zirconium Nanoparticle as Nanocarrier for Radioenhancer in X-Ray Dynamic Therapy and 177Lu-based Radioimmunotherapy of Lung Cancer.","authors":"Ahmad Kurniawan, Isa Mahendra, Asep Rizaludin, Marhendra Satria Utama, Ronny Lesmana, Fitria Dwi Ayuningtyas, Maria Rosa Dewanti, Dani Gustaman Syarif, Muhamad Basit Febrian","doi":"10.7150/ntno.101699","DOIUrl":"10.7150/ntno.101699","url":null,"abstract":"This study aimed to synthesize cetuximab (CTX) conjugated hydroxyapatite zirconium (HApZr-CTX) as a nanocarrier for active delivery of photosensitizer and therapeutic radionuclide. The system enabled targeted radioenhancer in X-ray dynamic therapy and radioimmunotherapy for lung cancer. The results showed that HApZr-CTX had the main characteristics of hydroxyapatite crystal in X-ray powder diffraction (XRD), with particle size twice bigger, according to DLS-PSA and TEM measurements. Cellular ROS generation was elevated almost three times in A549 cells after being treated using 125 µg/mL HApZr-CTX and irradiated with 5 Gy of X-ray photon compared to untreated cells. The viability of the treated lung cancer cell line decreased after exposure to external radiation. Moreover, as a radioimmunotherapy candidate, 177Lu was successfully loaded into HApZr-CTX nanocarrier and internalized in A549 more than half of the given dose after 0.5 h incubation. [177Lu]Lu-HApZr-CTX was primarily accumulated in the lung organs of healthy mice one-hour post-injection. In conclusion, HApZr-CTX nanoparticles have the potential to be used as a radioenhancer in X-ray dynamic therapy and radioimmunotherapy for lung cancer therapy.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"9 1","pages":"82-94"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11898717/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Affordable and Sustainable Biosensing Technology. 可负担和可持续的生物传感技术。

Nanotheranostics Pub Date : 2025-02-27 eCollection Date: 2025-01-01 DOI: 10.7150/ntno.106495

Pranjal Chandra

引用次数: 0

Structural Dynamics of OATP1A2 in Mediating Paclitaxel Transport Mechanism in Breast Cancer. OATP1A2介导乳腺癌紫杉醇转运机制的结构动力学

Nanotheranostics Pub Date : 2025-02-03 eCollection Date: 2025-01-01 DOI: 10.7150/ntno.103095

Rohit Kumar, Garima Singh, Yusuf Akhter, Gaurav Kaithwas, Ashish Kumar Agrawal, Sanjay Singh

{"title":"Structural Dynamics of OATP1A2 in Mediating Paclitaxel Transport Mechanism in Breast Cancer.","authors":"Rohit Kumar, Garima Singh, Yusuf Akhter, Gaurav Kaithwas, Ashish Kumar Agrawal, Sanjay Singh","doi":"10.7150/ntno.103095","DOIUrl":"10.7150/ntno.103095","url":null,"abstract":"Breast cancer remains a significant global health challenge, with drug resistance and poor bioavailability of chemotherapeutic agents like paclitaxel (PTX) presenting obstacles to effective treatment. This study investigates the potential role of the Solute Carrier Organic Anion Transporter Polypeptide 1A2 (OATP1A2) in PTX transport using computational approaches. We employed computational modeling, molecular docking, and molecular dynamics (MD) simulations to elucidate the structural dynamics of OATP1A2 and its interaction with PTX. The OATP1A2 structure was modeled using Phyre2, validated, and refined. Molecular docking revealed significant PTX interactions within the predicted binding site, with a binding affinity of -10.4 kcal/mol and initial hydrogen bonding with Arg656 and Gly560 and hydrophobic interaction with atGlu66, Phe65, Asn41, Ala203, Ile204, Phe329, Phe332, Ile336, Pro207, Ser337, Asn334. Contrary to our initial hypothesis of inward drug movement, MD simulation over 500 ns revealed an unexpected outward movement of PTX. The ligand shifted approximately 5.4 Å towards the extracellular side from its initial binding position. This observation suggests a more complex transport mechanism than initially anticipated. The protein-ligand complex exhibited stability throughout the simulation, with notable conformational changes. Our findings highlight the complex nature of OATP1A2-mediated transport and its potential limitations for PTX delivery. These results accentuate the complexity of transporter-mediated drug delivery and may inform future strategies for improving chemotherapeutic efficacy in breast cancer treatment.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"9 1","pages":"52-62"},"PeriodicalIF":0.0,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11898720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and Characterization of Carbon Nanodots from turmeric soot for anti-coliform and anti-oral bacterial applications and as anti-staphylococcal coatings. 姜黄烟尘碳纳米点的制备与表征及其抗大肠菌群、口腔细菌和葡萄球菌涂层的应用。

Nanotheranostics Pub Date : 2025-01-01 DOI: 10.7150/ntno.99825

Arjun Prakash, Manikandan Muthu, Gnanadeepam Raja, Judy Gopal

{"title":"Preparation and Characterization of Carbon Nanodots from turmeric soot for anti-coliform and anti-oral bacterial applications and as anti-staphylococcal coatings.","authors":"Arjun Prakash, Manikandan Muthu, Gnanadeepam Raja, Judy Gopal","doi":"10.7150/ntno.99825","DOIUrl":"10.7150/ntno.99825","url":null,"abstract":"In an era where chemical synthesis of nanomaterial is accounting for the generation of toxic wastes, leading to nanotoxicity, the present work focuses on the extraction of carbon nanodots from available natural sources such as turmeric smoke. The extracted carbon nanodots were characterized and their physical and chemical attributes were confirmed. The antibacterial property of the isolated carbon nanodots was tested against coliforms and oral bacteria. The results indicated that the carbon nanodots possessed highly versatile antibacterial activity. Carbon coatings were prepared by dip-dry method from the turmeric smoke-derived nanodots. The carbon-coated glass surfaces showed biofilm-repellant activity when exposed to pathogenic Staphylococcal cultures. The bactericidal and antibacterial activity of the in-house extracted carbon nanodots was proved. This study introduces an ecofriendly and simple rapid carbon nanomaterial synthesis process from smoke which can be extended for various other applications too.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"9 1","pages":"31-37"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11667564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic Potential of Feline Adipose-Derived Stem Cell Exosomes in the Treatment of Feline Idiopathic Cystitis: A Characterization and Functional Analysis of miRNA Content. 猫脂肪来源干细胞外泌体治疗猫特发性膀胱炎的治疗潜力：miRNA含量的表征和功能分析。

Nanotheranostics Pub Date : 2025-01-01 DOI: 10.7150/ntno.99383

Andrea Rubini, Federica Zanotti, Danilo Licastro, Giulia Calogero, Gisella Bettini, Cristiana Piccoli, Giuseppe Rubini, Luca Lovatti, Barbara Zavan

{"title":"Therapeutic Potential of Feline Adipose-Derived Stem Cell Exosomes in the Treatment of Feline Idiopathic Cystitis: A Characterization and Functional Analysis of miRNA Content.","authors":"Andrea Rubini, Federica Zanotti, Danilo Licastro, Giulia Calogero, Gisella Bettini, Cristiana Piccoli, Giuseppe Rubini, Luca Lovatti, Barbara Zavan","doi":"10.7150/ntno.99383","DOIUrl":"10.7150/ntno.99383","url":null,"abstract":"Feline Idiopathic Cystitis (FIC), is a chronic lower urinary tract condition in cats analogous to PBS/IC in women, which presents significant treatment challenges due to its idiopathic nature. Recent advancements in regenerative medicine highlight the potential of Adipose Tissue-Derived Stem Cells (ADSCs), particularly through their secretome, which includes mediators, bioactive molecules, and extracellular vesicles (EVs). Notably, exosomes, a subset of EVs, facilitate cell-to-cell communication and, when derived from ADSCs, exhibit anti-inflammatory properties and contribute to tissue regeneration. In this work, we aim to characterize the content of exosomes derived from feline ADSCs (fADSCs) to elucidate their mechanisms of action on recipient cells and assess their therapeutic potential for FIC. Exosomes were isolated from fADSCs and their microRNA (miRNA) content sequenced using Illumina technology. Our findings demonstrate that fADSC-derived exosomes harbor miRNAs that can induce regenerative processes, such as cell proliferation, immune modulation, angiogenesis, and anti-inflammatory responses. Key miRNAs identified include fca-miR-221, fca-let-7f-5p, fca-miR-337-5p, fca-miR-542-5p, fca-miR-24-3p, fca-miR-205, and fca-miR-23a, which promote proliferative, angiogenic, differentiation, and regenerative mechanisms. Additionally, miRNAs with anti-inflammatory effects, such as fca-miR-193a-5p and fca-miR-127-3p, and those positively regulating the immune system, including fca-let-7a-5p and fca-miR-chrC1_18846-5p, were identified. Of particular interest, fca-miR-219-5p (has-miR-6766-3p) has been reported to suppress liver fibrosis.These results underline the therapeutic potential of fADSC-derived exosomes in treating FIC and suggest innovative strategies for feline veterinary medicine.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"9 1","pages":"38-51"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11667565/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0