Defence Life Science Journal最新文献

{"title":"Food Safety and Biosecurity","authors":"A. Goel","doi":"10.14429/dlsj.4.15203","DOIUrl":"https://doi.org/10.14429/dlsj.4.15203","url":null,"abstract":"Food, cloth and shelter have been the three basic amenities of human being since ages. The 21st century is well known for the golden era of science and technology. Despite of tremendous progress in agriculture, medical sciences and food processing, still about 770 million people (about one in every nine people) suffer from chronic hunger globally. About 600 million people (about one in every ten people) fall ill due to food borne disease and about 4,20,000 people die every year due to food borne diseases. Hence, food availability and food safety becomes an area of paramount importance. Biosecurity in reference to food safety is also equally important because of burgeoning cross border trade globally. A major thrust is being given on the integrated surveillance to monitor and manage the risks along the entire food chain. Hence, the current issue of Defence Life Science Journal is focussed on the food safety and biosecurity. Overall, the living standard of people has improved over the years due to tremendous developments in the field of science and technology. The modern tools of genetic engineering have paved the new ways for enhanced crop production with better quality to meet out the food requirement of the world’s growing population. However, it is very important to ensure the food safety to achieve this goal. There are a lot of concerns about the genetically modified crops and microorganisms, hence their long term impacts on the environment need to be assessed scrupulously. As foodborne diseases pose a heavy toll on the health and economy of word, hence, it is of utmost importance to maintain the sanitary practices in food chain to obviate the microbial contamination and food poisoning. The term bioseuirty, in general is applied to prevent the loss, theft or escape of potentially dangerous pathogens from the research laboratory which can cause public health harm. However, in reference to food safety, biosecurity is the imperative approach to ensure food safety, which interlinks health, environment, security and trade. Thus, biosecurity is a holistic system covering food safety, zoonoses, transboundary introduction of diseases and pests, and release of living modified organisms (LMOs) and their products (e.g. genetically modified organisms or GMOs). In this era of globalisation, food pathogens during transboundary movements can pose high risk to food chain in the new regions where they never existed. However, the adequate biosecurity management at national and international level can ensure the food safety during the international trade. The potential biothreat agents also pose a potential threat to global community. Several of these pathogens can be part of food chain. Hence, proper management of biosecurity as well as rapid detection and diagnostic systems are also required for the society. Moreover, it is very important to make people aware about the food safety and biosecurity. Therefore, a special issue of Defence Life Science Journal (DLS","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41820520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of Biocontrol Agents in Plant Disease Control for Improving Food Safety","authors":"Anju Sehrawat, S. Sindhu","doi":"10.14429/dlsj.4.14966","DOIUrl":"https://doi.org/10.14429/dlsj.4.14966","url":null,"abstract":"Plant disease control is mainly based on extraneous application of pesticides to improve agriculture productivity. However, only a part of applied pesticides is used for killing of pathogens and pests. Large part of applied pesticides remains either as residual pesticide or gets volatilized or leached resulting in ecological and environmental problems, and human health hazards. The increased consumer demands for safe food have invigorated research on development of safe and ecofriendly biopesticides. The use of microorganisms for biological control of pests is considered as a pragmatic approach which can drastically lessen the adverse outcomes of agrochemicals in soil. Rhizospheric microorganisms isolated from various crops produce different antagnostic compounds and inhibit the growth of various phytopathogens and insect pests. Moreover, in several plants, hormones like salicylic acid, jasmonic acid and ethylene contribute towards induction of both, systemic acquired as well as induced systemic resitance. In this article, antagonistic rhizosphere microorganisms have been explored for control of phytopathogens. Further, recent advances in field of biopesticides using rhizosphere microorganisms under field conditions is discussed for improvingcrop productivity in sustainable agriculture","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41595023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food Irradiation An Established Food Processing Technology for Food Safety and Security","authors":"Rita Singh, Antaryami Singh","doi":"10.14429/dlsj.4.14397","DOIUrl":"https://doi.org/10.14429/dlsj.4.14397","url":null,"abstract":"Food irradiation is a well-established and effective technology for food processing and preservation. The technology aids in reducing food losses and ensuring food safety by elimination of pathogens and parasites causing illness and death. Radiation treatment can be applied to agricultural produce and animal food products to get extended shelf life with improved microbiological safety and quality. Irradiating food can greatly reduce illness from foodborne pathogens thereby preventing morbidity and mortality. Various national and international food and health organisations have endorsed and supported the safety of food and foodstuffs subjected to ionising radiation based on the research and testing data of more than 50 year. A review is presented on the historical developments of food irradiation technology, radiation sources for treatment of food and, the safety and wholesomeness of foods processed by ionising radiation.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46324924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicity Assessment of Biologically Degraded Product of Textile Dye Acid Red G","authors":"P. Faldu, V. Kothari, C. Kothari, Jalpa K. Rank, A. Hinsu, R. Kothari","doi":"10.14429/dlsj.4.14972","DOIUrl":"https://doi.org/10.14429/dlsj.4.14972","url":null,"abstract":"Azo dyes are of environmental concern due to their recalcitrant nature. Several azo dyes and their decolorized and degraded products exert toxic and mutagenic effects on the flora and fauna. The toxic properties of these azo dyes are due to nature and position of the substitution with respect to the aromatic rings and amino nitrogen atoms. Several studies have thus far been emphasized on biodegradation of azo dye pollutants, though role of their biodegraded product is rarely studied. Given a lack of this understanding, we have analyzed the effects of degraded products of a di-azo textile dye Acid Red G by newly isolated bacterial species, Pseudomonas aeruginosa PFK10 and Brevibacillus choshinensis PFK11. The genotoxicity and cytotoxicity of Acid Red G and their degraded products were tested on HeLa cell line and Human lymphocyte cell, respectively. The data of MTT assay has been shown that activity of degraded products of the Acid Red G were comparable to their parent dye. But chromosome aberration assay and sister chromatid exchange assay did not show any significant changes in chromosomes as compared to positive control mitomicine.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46835756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ELISA Based Detection of Botulinum Neurotoxin Type F in Red Meat and Canned Fish","authors":"S. K. Yadav, Monika Singh, S. Ponmariappan","doi":"10.14429/dlsj.4.14915","DOIUrl":"https://doi.org/10.14429/dlsj.4.14915","url":null,"abstract":"Botulinum neurotoxin (BoNT) is the most toxic protein molecule so far known to animals and humans, due to its extreme toxicity, CDC has been listed as category ‘A' Biological warfare agents. In India, there is no commercial detection system available for the detection of botulism. The present study was aimed to develop an ELISA based detection system for botulinum neurotoxin serotype F in red meat and canned fish. Polyclonal antibodies were generated against the recombinant BoNT/F LC protein and IgG was purified. Sandwich ELISA system was successfully optimized with LOD of ~ 7.8 ng/ml in PBS. Similarly, different concentrations of BoNT/F were spiked in red meat and canned fish and extracted the toxin and the detection LOD was achieved for red meat ~ 62 ng/ml and for canned fish ~ 31 ng/ml. The developed detection system is highly specific. The developed assay will be useful for the screening of botulinum toxins in a large number of food samples.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41767409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One step Purification and Characterisation of Abrin Toxin from Abrus Precatorius Seeds","authors":"Swati Banger, Rita Singh, N. Tripathi, V. Pal, A. Goel","doi":"10.14429/dlsj.4.14967","DOIUrl":"https://doi.org/10.14429/dlsj.4.14967","url":null,"abstract":"Abrin is a plant toxin obtained from Abrus precatorius seeds. It belongs to the type II ribosomal inactivating proteins (RIPs) consisting of two chains namely, catalytically active A chain and sugar binding B chain linked by a single disulphide bond.  Due to high toxicity of abrin, its exposure or consumption can lead to serious public health problems. In the present work, we have extracted and purified the abrin toxin from Abrus precatorius seeds. The toxin was purified using a single step anion exchange chromatography. The purified protein was characterized by SDS-PAGE and MALDI- TOF to confirm its purity. The toxicity of purified abrin toxin was also confirmed by injecting the toxin in mice.  The purified protein was further used to raise antibodies in mice and characterized by indirect Enzyme Linked Immunosorbent Assay. The results of present study established the use of single step ion exchange chromatography to purify abrin toxin for further development of its detection system.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42970034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Production of Protective Antigen, a Potent Diagnostic Protein of Bacillus anthracis, the Causative Agent of Anthrax","authors":"Manoj Kumar, N. Puranik, N. Tripathi, V. Pal, A. Goel","doi":"10.14429/dlsj.4.15132","DOIUrl":"https://doi.org/10.14429/dlsj.4.15132","url":null,"abstract":"Protective antigen (PA) produced by Bacillus anthracis is a highly immunogenic protein. Therefore, it has significant importance in serodiagnosis as well as a vaccine candidate for anthrax. In the present study, codons for PA gene were optimised and synthesised for its expression in Escherichia coli. Various expression conditions were optimised for scaled up production of rPA. The final yield of affinity chromatography purified protein was 40.8 mg/l during batch fermentation. For further purification, affinity purified protein was diafiltered and subjected to anion exchange chromatography. SDS-PAGE and Western blot was used to characterise the purified rPA protein. The diagnostic potential of purified rPA was evaluated in Western blot using standards reference serum AVR 801 and cutaneous anthrax clinical sera. The results of the present study established the optimum production of rPA in E. coli after codon optimisation for its subsequent use in diagnosis of anthrax infection.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45813408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implications of Biosecurity in Food Safety","authors":"V. Pal, N. Tripathi, A. Goel","doi":"10.14429/dlsj.4.14917","DOIUrl":"https://doi.org/10.14429/dlsj.4.14917","url":null,"abstract":"Owing to growing population of world, efforts are being made to maximise food production. Food safety should not be compromised to meet the food requirement of increasing population. Biosecurity is the imperative approach to ensure food safety. This is a holistic approach that interlinks health, environment, security and trade. Increased incidents of foodborne diseases led to promotion of biosecurity as a major priority policy worldwide to curtail such incidents and ensure food safety. Microbial risk management is an essential component of food safety. National biosecurity programmes are essentially required to identify the prospective modes of introduction and spread of a disease in a country or region and to specify the control measures to curtail the risk associated with the disease. International standards for various biosecurity sectors are set mainly by Codex Alimentarius Commission, the World Organisation for Animal Health and Commission on Phytosanitary Measures, which are implemented through the Sanitary and Phytosanitary Agreement, 1995 of World Trade Organisation. Agricultural biosecurity is of utmost importance in the countries that are large crop and animal producers, and these countries are at risk from alien pests and pathogens. Adequate biosecurity programmes are essential in all the countries to protect global environment, agriculture and biodiversity. Developing countries, particularly with large populations aiming maximised food production require stringent biosecurity approaches to provide safe and nutritious food to the people.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44930891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Normal Body Mass Index and Heart Rate Variability","authors":"J. Jain, Ranjan Maheshwari","doi":"10.14429/DLSJ.4.13732","DOIUrl":"https://doi.org/10.14429/DLSJ.4.13732","url":null,"abstract":"The autonomic nervous system (ANS) works in synergy with the Endocrine system that affects the body-mass and height and in turn the body mass index (BMI). The ANS activities are often assessed with one index that is heart rate variability (HRV). Reduced HRV has been reported in underweight (low BMI) and overweight (high BMI) individuals, but there is scarce information available on the relationship between normal BMI and HRV. Further, as per WHO expert consultation report, the Asian population has higher percentage of body fat than their European counterparts, therefore Asian people may have greater risk factors for type 2 diabetes and cardiovascular disease even below the existing upper edge of normal BMI. Thus it was recommended by WHO to consider the intermediate cutoff points within the normal BMI range as 18.5 Kg/m2, 20 Kg/m2, 23 Kg/m2, and 25 Kg/m2 for the Asian population. Therefore, the present study was aimed to investigate ANS activity among intermediate cutoff points of normal BMI using HRV. Seventy young individuals participated in the non-invasive and benign study. Subjects were divided into three groups based on their BMI as per the recommendation of the WHO report; NB1 (18.5<BMI≤20), NB2 (20<BMI<23) and NB3 (23<BMI<25). For all the subjects, 10 min of electrocardiogram was recorded and short term HRV analysis was carried out. Student t test was carried out to find the significance of study parameters in BMI groups. The BMI was correlated with HRV measures using Spearman’s correlation method. Statistically significant negative correlation was found between BMI and various HRV parameters. The sympathovagal balance was comparable in NB1 and NB2 group whereas it shifted towards sympathetic dominance in NB3 group. Higher sympathetic activity for BMI greater than 23 in Indian youth may lead to predictability of risks associated with overweight and obesity","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46060054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous Sachharification and Fermentation of Rice Residues and its Comparative Analysis for Bioethanol Production","authors":"G. Sinha, S. Tiwari, S. Jadhav","doi":"10.14429/DLSJ.4.14188","DOIUrl":"https://doi.org/10.14429/DLSJ.4.14188","url":null,"abstract":"Energy consumption has inflated steadily over the last century because the world population has fully grown and additional countries became industrialised. Bioethanol is an alcohol produced by fermentation of plant biomass, containing carbohydrate and its production depends upon feedstock availability, variability, and sustainability. The selection of feedstock and its pretreatment is an important part of bioethanol production process. In present work, the exploration of the potential of agro-waste rice residues such as, rice bran and rice husk was done, because it contains sufficient amount of carbohydrate which can be ferment into bioethanol. The aim of the research was also to investigate how different pretreatment methods with moderate conditions differ in hydrolysis and fermentation efficiencies. Pretreatment plays an important role in the hydrolysis of cellulose and lignocellulose. It was found that biological pretreatment was a most effective method in terms of production of bioethanol and it enhances the production as well as fermentation efficiency.","PeriodicalId":36557,"journal":{"name":"Defence Life Science Journal","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46542596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}