ELISA Based Detection of Botulinum Neurotoxin Type F in Red Meat and Canned Fish

Q3 Pharmacology, Toxicology and Pharmaceutics
S. K. Yadav, Monika Singh, S. Ponmariappan
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引用次数: 2

Abstract

Botulinum neurotoxin (BoNT) is the most toxic protein molecule so far known to animals and humans, due to its extreme toxicity, CDC has been listed as category ‘A' Biological warfare agents. In India, there is no commercial detection system available for the detection of botulism. The present study was aimed to develop an ELISA based detection system for botulinum neurotoxin serotype F in red meat and canned fish. Polyclonal antibodies were generated against the recombinant BoNT/F LC protein and IgG was purified. Sandwich ELISA system was successfully optimized with LOD of ~ 7.8 ng/ml in PBS. Similarly, different concentrations of BoNT/F were spiked in red meat and canned fish and extracted the toxin and the detection LOD was achieved for red meat ~ 62 ng/ml and for canned fish ~ 31 ng/ml. The developed detection system is highly specific. The developed assay will be useful for the screening of botulinum toxins in a large number of food samples.
基于ELISA法检测红肉和鱼罐头中F型肉毒毒素
肉毒杆菌神经毒素(BoNT)是迄今为止已知的对动物和人类毒性最大的蛋白质分子,由于其极高的毒性,美国疾病控制与预防中心已将其列为A类生物战制剂。在印度,没有可用于肉毒杆菌中毒检测的商业检测系统。本研究旨在建立一种基于ELISA法检测红肉和鱼罐头中F型肉毒毒素的方法。制备了针对重组BoNT/F LC蛋白的多克隆抗体,并纯化了IgG。夹心酶联免疫吸附测定系统在PBS中检出限为~ 7.8 ng/ml。同样,在红肉和罐头鱼中加入不同浓度的BoNT/F提取毒素,红肉和罐头鱼的检出限分别为62 ng/ml和31 ng/ml。开发的检测系统具有高度的特异性。该方法可用于大量食品样品中肉毒杆菌毒素的筛选。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Defence Life Science Journal
Defence Life Science Journal Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
0.80
自引率
0.00%
发文量
26
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