Journal of Inorganic Biochemistry最新文献

{"title":"Synthesis and anticancer activity of six tricyclohexyltin cinnamate complexes","authors":"Zhiyu Pan ,&nbsp;Wen Zhong ,&nbsp;Yanping Li ,&nbsp;Shijie Lin ,&nbsp;Shaofeng Chen ,&nbsp;Wei Tian","doi":"10.1016/j.jinorgbio.2025.112991","DOIUrl":"10.1016/j.jinorgbio.2025.112991","url":null,"abstract":"<div><div>Six tricyclohexyl tin cinnamate complexes (<strong>C1</strong> ∼ <strong>C6</strong>) were successfully synthesized and were characterized. The crystal structures of <strong>C1</strong>, <strong>C2</strong>, <strong>C4</strong>, and <strong>C5</strong> were determined. Among them, complexes <strong>C1</strong>, <strong>C2</strong> and <strong>C5</strong> form a one-dimensional infinite chain structure through Sn<img>O interactions or O-H…O hydrogen bonds. All complexes were tested for their inhibitory activity against human cancer cell lines A549, HepG2, and MDA-MB-231. The results showed that the <strong>C2</strong> complex demonstrated the most significant inhibitory effect on HepG2 cells, with an IC₅₀ value of 1.31 ± 0.47 μM. Preliminary studies indicate that the <strong>C2</strong> complex induces a reduction in mitochondrial membrane potential in HepG2 cells, triggering apoptosis via the mitochondrial pathway accompanied by cell cycle arrest at the G2 phase. The DNA binding activity of <strong>C2</strong> was investigated using ultraviolet-visible, fluorescence competition assays and molecular docking, revealing that <strong>C2</strong> can effectively intercalate the DNA groove.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"272 ","pages":"Article 112991"},"PeriodicalIF":3.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-heterocyclic carbene gold(I) derivatives with long aliphatic side chains as potential anticancer agents in colon cancer","authors":"Javier Quero ,&nbsp;Adrián Alconchel ,&nbsp;Sara Ortega ,&nbsp;Seyed Hesamoddin Bidooki ,&nbsp;Mª. Concepción Gimeno ,&nbsp;Mª. Jesús Rodriguez-Yoldi ,&nbsp;Elena Cerrada","doi":"10.1016/j.jinorgbio.2025.112987","DOIUrl":"10.1016/j.jinorgbio.2025.112987","url":null,"abstract":"<div><div>Mild hyperthermia has emerged as a powerful tool in cancer therapy, prompting the development of materials that respond to heat with enhanced therapeutic action. Gold(I)-NHC complexes are emerging as promising anticancer agents due to their stability, tunability, and ability to inhibit sulfur- and selenium-dependent enzymes overexpressed in tumors. In this study, we synthesised carbene–gold(I) derivatives bearing fluorous and hydrocarbon chains to assess the role of polyfluorinated groups and the impact of mild hyperthermia (41 °C) on their cytotoxic activity. The compounds exhibited significant antiproliferative effects against Caco-2/TC7 colon carcinoma cells at both 37 °C and 41 °C. This activity may be associated with alterations in the levels of ROS (reactive oxygen species) within the cells and the activity of TrxR (thioredoxin reductase), resulting in modifications to the intracellular redox state and subsequent disruptions to the cell cycle. Under hyperthermic conditions, cytotoxicity was further enhanced via mitochondrial depolarization and activation of caspase-3-mediated apoptosis. Notably, fluorinated complexes displayed superior cytotoxicity compared to their alkylated analogues, highlighting the relevance of polyfluorinated chains in boosting therapeutic efficacy under heat-triggered conditions.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"272 ","pages":"Article 112987"},"PeriodicalIF":3.8,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of σ and π reaction channels in hydrogen atom transfer reactions","authors":"Faiza Ahsan ,&nbsp;Mursaleem Ansari ,&nbsp;Johannes E.M.N. Klein ,&nbsp;Marcel Swart","doi":"10.1016/j.jinorgbio.2025.112989","DOIUrl":"10.1016/j.jinorgbio.2025.112989","url":null,"abstract":"<div><div>C(sp³)–H bond activation mechanisms typically involve σ- and π-channel pathways, as characterized by FeOH (or FeOC) angles of ca. 180° and 120°, respectively. It is well known that the preference for either the σ- or π-channel depends on the spin state, but doubts exist on what would be characteristic values for the FeOX (X = H or C) angles. Here we study the oxidation of methane and ethane mediated by an Fe(IV)oxo model complex through density functional theory. A systematic comparison of dispersion-corrected B3LYP (B3LYP-D2, B3LYP-D3, B3LYP-D3BJ, B3LYP-D4) and the uncorrected counterpart (B3LYP) was conducted to evaluate the role of dispersion interactions in both gas and solvent phases. Our results reveal that dispersion corrections significantly influence barriers at transition states (TSs), particularly in the solvent phase, where dispersion contributions enhance stabilization of TS structures. The σ-channel pathway dominates for high spin (<em>S</em> = 2), while intermediate spin (<em>S</em> = 1) states favor the π-channel. Dispersion effects were found to be more pronounced for ethane, where larger non-covalent interactions between the substrate and Fe(IV)oxo complex arise. The FeOX angles vary substantially depending on the choice of dispersion correction, and between gas phase and solution phase. Indeed, for the reaction with ethane the FeOX values of the σ-channel approach values that are typically associated with the π-channel. Fortunately, the Spin-Resolved Charge Displacement Function provides a clear visual tool to distinguish the two channels. These insights advance the understanding of hydrocarbon functionalization by high-valent iron-oxo species, with implications for synthetic catalyst design in homogeneous and enzymatic catalysis.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"272 ","pages":"Article 112989"},"PeriodicalIF":3.8,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144589303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring Biginelli hybrids in the AI-driven development of ruthenium complexes: Anticancer activity, DNA/HSA binding study, impacts on apoptosis and BCL-2/BCL-XL suppression","authors":"Ana Rilak Simović ,&nbsp;Dejan Milenković ,&nbsp;Dragana Šeklić ,&nbsp;Milena Jovanović ,&nbsp;Emilija Milović ,&nbsp;Milica Međedović ,&nbsp;Milan Vraneš ,&nbsp;Nenad Janković","doi":"10.1016/j.jinorgbio.2025.112988","DOIUrl":"10.1016/j.jinorgbio.2025.112988","url":null,"abstract":"<div><div>Ruthenium-arene complexes are promising alternatives to platinum-based anticancer drugs due to their unique chemical properties and lower toxicity. These complexes typically have a “half-sandwich” structure where an arene ligand stabilizes the ruthenium center. This study aimed to design tetrahydropyrimidines (<strong>THPM</strong>) and their ruthenium <em>p</em>-cymene complexes with anticancer potential using deep learning models for binding affinity prediction. Ten compounds with binding energies lower than −31.3 kJ/mol were selected for further investigation. Molecular docking studies revealed that the ruthenium complexes <strong>5j</strong> and <strong>5g</strong> exhibited the most pronounced activity against <em>Caspase 3</em>. These complexes showed significant cytotoxic activity and selectivity against primary and metastatic cancer cell lines, inducing apoptosis as the preferred mode of cell death through the modulation of <em>Caspases</em> expression. The <em>K</em>_b and <em>K</em>_sv values for the interaction of <strong>5j</strong> with EB-DNA, Hoechst-DNA, HSA, HSA-Eosin Y, and HSA-Ibuprofen were higher compared to those of <strong>5m</strong>. Binding constants in the presence of the tested <strong>BIO-ILs</strong> followed the order <strong>IL1</strong> (ethanoate) &lt; <strong>IL2</strong> (butanoate) &lt; <strong>IL3</strong> (hexanoate), correlating with the length of the alkyl chain in the anions and the lipophilicity of the tested <strong>BIO-ILs</strong>. The best result of this study was that treatment with <strong>5g</strong> induced apoptosis and reduced the expression of anti-apoptotic markers (<em>BCL-2</em> and <em>BCL-XL</em>), which are associated with resistance acquisition. The research outcomes emphasize the integration of computational methods with experimental validation, underscoring the importance of collaboration between AI technologies and traditional chemistry in drug discovery.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"272 ","pages":"Article 112988"},"PeriodicalIF":3.8,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144589043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coordination complexes of lanthanide(III) derived from 2-(pyridin-2-yl)-1H-benzo[d]imidazole (bimpy) and 2,2′-bipyridine (bpy): Spectroscopic analysis and cytotoxic evaluation","authors":"Luis E. Romero-Gutiérrez ,&nbsp;Miguel A. Vergara-Rodríguez ,&nbsp;J. Antonio Cruz-Navarro ,&nbsp;Sharon Rosete-Luna ,&nbsp;Aracely López-Monteon ,&nbsp;Angel Ramos-Ligonio ,&nbsp;Rodolfo Peña-Rodríguez ,&nbsp;Silvia Castillo-Blum ,&nbsp;Raúl Colorado-Peralta","doi":"10.1016/j.jinorgbio.2025.112985","DOIUrl":"10.1016/j.jinorgbio.2025.112985","url":null,"abstract":"<div><div>Seven novel coordination complexes (<strong>1</strong>–<strong>7</strong>) derived from trivalent lanthanides (La, Nd, Sm, Eu, Gd, Tb, Dy) and bidentate ligands 2,2′-bipyridine (<em>bpy</em>) and 2-(pyridin-2-yl)-1<em>H</em>-benzo[<em>d</em>]imidazole (<em>bimpy</em>) were synthesised. A series of analytical and spectroscopic techniques, including high-resolution mass spectrometry, unambiguously characterised all complexes. Using ligand mixtures to obtain coordination complexes with anticancer activity has shown promising results. Therefore, additional experiments were performed to evaluate the cytotoxic activity in non-transformed cells (NIH/3 T3 and J774A.1) and transformed cells (HeLa, Caco-2 and MCF-7), as well as erythrocyte lysis experiments in human red blood cells. The results revealed moderate but selective cytotoxicity against the studied cell lines. Additional PBE0/def2-SVP computational studies were carried out to elucidate the coordination number, geometry and structural organisation in agreement with the experimental data.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"272 ","pages":"Article 112985"},"PeriodicalIF":3.8,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144570132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Visible light-activated cobalt phthalocyanine/UiO-67 composite: A novel approach to photodynamic antibacterial therapy","authors":"Anadil Gul ,&nbsp;Munir Ahmad ,&nbsp;Rizwan Ullah ,&nbsp;Kamran Ullah ,&nbsp;Yan Kang ,&nbsp;Wenchao Liao","doi":"10.1016/j.jinorgbio.2025.112986","DOIUrl":"10.1016/j.jinorgbio.2025.112986","url":null,"abstract":"<div><div>Metallophthalocyanines (MPcs) are highly promising photosensitizers owing to their exceptional photoelectronic properties, yet their practical photocatalytic applications are often limited by strong π–π self-aggregation. In this study, we report a facile in situ strategy to incorporate cobalt phthalocyanine (CoPc) into the porous matrix of UiO-67 metal-organic frameworks (MOFs), effectively suppressing aggregation and enhancing photo-activity. The resulting composite, UCoP2–32, exhibited outstanding antibacterial photodynamic therapy (APDT) performance under visible light, achieving over 96 % inactivation of methicillin-resistant <em>Staphylococcus aureus</em> (MRSA) within 12 min of light exposure. Photophysical studies confirmed the efficient generation of both type-I and type-II reactive oxygen species (ROS), with superoxide and hydroxyl radicals predominating, and electron spin resonance (ESR) analysis supported this mechanism. Moreover, UCoP2–32 demonstrated excellent biocompatibility with minimal hemolysis (∼6.4 % at 110 μM) and structural stability. This work provides a robust and broadly applicable strategy for integrating MPcs into MOFs, offering significant potential for the development of high-performance photocatalytic materials in antimicrobial and other light-driven applications.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"272 ","pages":"Article 112986"},"PeriodicalIF":3.8,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144623487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yeast hexokinase inactivation by lead and its reversion by magnesium as one possible aspect of lead toxicity","authors":"Rogelio Rodríguez-Sotres ,&nbsp;Axel Blokesch ,&nbsp;Carolina Moreno-Galván ,&nbsp;Adrián Rodríguez-Vargas ,&nbsp;Juvencio Robles ,&nbsp;Minerva Martínez-Alfaro","doi":"10.1016/j.jinorgbio.2025.112983","DOIUrl":"10.1016/j.jinorgbio.2025.112983","url":null,"abstract":"<div><div>Epidemiological studies suggest a positive association between blood lead levels and fasting blood glucose levels (BGL). The link between glucose homeostasis and Pb exposure is unclear. BGL is the result of multiple processes involving many enzymes. One of the critical initial steps in cellular glucose metabolism is its phosphorylation by ATP, catalyzed by hexokinase (HK) or glucokinase in hepatocytes. This phosphorylation prevents glucose inside cells from diffusing back out and commits glucose to further intracellular processing.</div><div>The aim of this study is to analyze the effect of lead and other divalent metals in hexokinase (HK) enzyme activity <em>in vitro</em> to understand the toxic effect of lead on a Mg²⁺-dependent enzyme. The highest HK activity was observed in the presence of Mg in absence of Pb.</div><div>Our results show that lead inhibits HK in a time-dependent fashion, thus requiring several minutes of preincubation with Pb but without glucose. A modified non-competitive inhibition model is the best to describe this loss of activity. Mg is the only metal that partially reverted the lead dependent loss of HK activity. These results suggest that lead forms stable metal complexes which interact with different protein targets, some of them displace for Mg and others available in protein conformation without glucose. Data calculated at the DFT level of theory show that all Metal-ATP complexes promote the glucose phosphorylation. Inhibition does not seem to depend on the formation of a PbATP complex.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"272 ","pages":"Article 112983"},"PeriodicalIF":3.8,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144597561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic profiling of parthanatos and the neuroprotective potential of sodium perborate tetrahydrate","authors":"Merve Gulsen Bal Albayrak ,&nbsp;Sevinc Yanar ,&nbsp;Tuğcan Korak ,&nbsp;Gurler Akpinar ,&nbsp;Murat Kasap","doi":"10.1016/j.jinorgbio.2025.112984","DOIUrl":"10.1016/j.jinorgbio.2025.112984","url":null,"abstract":"<div><div>Parthanatos is a caspase-independent form of programmed cell death triggered by PARP-1 overactivation and mitochondrial dysfunction, implicated in neurodegenerative diseases. In this study, we performed a proteomic analysis of SH-SY5Y neuronal cells undergoing parthanatos and evaluated the neuroprotective effects of sodium perborate tetrahydrate (SPT), an inorganic boron-containing compound. LC-MS/MS analysis revealed significant alterations in mitochondrial respiration, DNA repair, and inflammatory signaling pathways. Proteins such as MT-CO2, CYC1, POLR2L, and SLC25A5 -implicated in Parkinson's and Huntington's disease pathways- were found to be dysregulated. SPT pre-treatment led to reduced PARP-1 activation, decreased DNA fragmentation, and improved cell viability. These findings suggest that boron-containing inorganics, as metalloids, can modulate molecular targets involved in neuronal survival. Our study contributes novel experimental evidence on the biochemical effects of boron compounds in neurodegenerative processes, complementing current research in metalloid-biomolecule interactions and supporting their potential utility in therapeutic development for neurodegenerative diseases.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"271 ","pages":"Article 112984"},"PeriodicalIF":3.8,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cadmium(II) complexes with pyridine/dicarboxylic acid ligands as promising molecules for the treatment of oral candidiasis","authors":"Diana Liz Jimenez ,&nbsp;Débora Taisa Keller da Silva ,&nbsp;Talita da Paz Costa Sauda ,&nbsp;Adriana Araújo de Almeida-Apolonio ,&nbsp;Emanuele P.O. Roque ,&nbsp;Pamella Fukuda de Castilho ,&nbsp;João Víctor de Andrade dos Santos ,&nbsp;Sidnei M. Silva ,&nbsp;Lucas Pizzuti ,&nbsp;Amilcar M. Junior ,&nbsp;Victor M. Deflon ,&nbsp;Kelly Mari Pires de Oliveira ,&nbsp;Gleison Antônio Casagrande","doi":"10.1016/j.jinorgbio.2025.112980","DOIUrl":"10.1016/j.jinorgbio.2025.112980","url":null,"abstract":"<div><div>Overgrowth of <em>Candida</em> spp., known for its strong adhesion to biotic and abiotic surfaces, makes treatment difficult in denture stomatitis. To address this challenge, two novel cadmium(II) complexes containing 2,5-pyridinedicarboxylic acid (H₂L) (complex <strong>1</strong> [Cd(HL)(H₂O)(DMSO)I]₂ and [Cd(HL)(H₂O)(bipy)I] complex <strong>2</strong>), were synthesized and fully characterized by X-ray diffractometry, HRMS-ESI (+) spectrometry, ¹H and ¹³C NMR, FT-IR and elemental analysis. These complexes were evaluated for their antifungal, antibiofilm, hemolytic, and mutagenic properties. The interaction of these complexes with established antifungal agents was also investigated. Both complexes demonstrated remarkable antifungal activity, particularly against <em>C. albicans</em> and <em>C. krusei</em>, with MIC ranging from 62.5 (97.91 μM) to 0.48 (0.75 μM) μg/mL and MFC from 125 (195.82 μM) to 3.80 (5.95 μM) μg/mL. Furthermore, these complexes effectively inhibited <em>C. krusei</em> biofilm formation on prosthetic acrylic resin test specimens, with complex <strong>2</strong> showing superior activity. The complexes also displayed synergistic effects with fluconazole, furthermore, sorbitol assays have shown that cell wall is one of the targets of the tested complexes. Importantly, hemolysis assays indicated that the complexes were non-cytotoxic to human erythrocytes, and mutagenicity assays confirmed their non-mutagenic nature. These findings suggest that the synthesized cadmium(II) complexes, particularly complex <strong>2</strong>, possess significant potential as therapeutic agents for the treatment of denture stomatitis and oral candidiasis in general.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"271 ","pages":"Article 112980"},"PeriodicalIF":3.8,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Near-infrared activation of upconversion platforms for non-redox-dependent release of Pt(II)","authors":"Marc-Ricard Batten ,&nbsp;Josep Antoni Gutiérrez-Orgaz ,&nbsp;Fernando Eduardo Maturi ,&nbsp;Luís Dias Carlos ,&nbsp;Helena Oliveira ,&nbsp;Jordi Hernando ,&nbsp;Fernando Novio ,&nbsp;Antonio Rodríguez-Diéguez ,&nbsp;Mercè Capdevila ,&nbsp;Òscar Palacios ,&nbsp;Pau Bayón","doi":"10.1016/j.jinorgbio.2025.112982","DOIUrl":"10.1016/j.jinorgbio.2025.112982","url":null,"abstract":"<div><div>Upconversion nanoparticles (UCNPs) are a class of interesting nanomaterials with unique multi-photon excitation photoluminescence properties, and they have been intensively explored as novel contrast agents for biomedical imaging and drug delivery. The development of photoinduced drug-release devices has been intensively developed in the last years, specially using UCNPs due to their properties to absorb single-band near infrared (NIR) light and subsequently emit high-energy UV-to-visible light which could photoactivate several prodrugs. Some examples of Pt(II) release have been described, all of them from Pt(IV) complexes taking advantage of the Pt(IV)/(II) redox couple. In this work, NIR light-responsive LiYF₄:Yb/Tm UCNPs are presented as carrier systems to exert photoinduced Pt(II) drug release. For this, the surface of UCNPs were coated with an amphiphilic polymer to convert hydrophobic nanoparticles into hydrophilic and to load novel Pt(II) complexes. It is demonstrated that NIR radiation-induced Pt(II) drug release can be achieved without the need to use the Pt(IV)/(II) redox couple as a trigger. In this way, under NIR excitation, UCNPs can transform NIR irradiation into UV radiation which causes direct Pt(II) drug release in a spatial and temporal control manner. The release process has been monitored in real-time. Two platforms containing two different Pt(II) complexes have been studied, both showing similar results in terms of the enhancement of toxicity caused by the increase in Pt(II) concentration. Furthermore, a significant improvement of cytotoxicity against melanoma A375 cells was observed after irradiation of these platforms, confirming the feasibility of the proposed upconversion process to release Pt(II).</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"271 ","pages":"Article 112982"},"PeriodicalIF":3.8,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144518948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}