Research in Plant Disease最新文献_第7页

Occurrence of Viruses and Viroids in Chrysanthemum Plants (Dendranthema morifolium) Cultivated in Yesan-gun, Chungcheongnam-do in Korea 韩国忠清南道野山郡栽培菊花中病毒和类病毒的发生

Research in Plant Disease Pub Date : 2022-12-31 DOI: 10.5423/rpd.2022.28.4.237

Yoon Hyun Bang, E. Song, Younghye Lee, K. Ryu

{"title":"Occurrence of Viruses and Viroids in Chrysanthemum Plants (Dendranthema morifolium) Cultivated in Yesan-gun, Chungcheongnam-do in Korea","authors":"Yoon Hyun Bang, E. Song, Younghye Lee, K. Ryu","doi":"10.5423/rpd.2022.28.4.237","DOIUrl":"https://doi.org/10.5423/rpd.2022.28.4.237","url":null,"abstract":"Chrysanthemum plants are one of the most economically important plants in South Korea. Both virus and viroid can cause diseases and economic damage to the plants. In this study, we investigated the detection of seven viruses and two viroids in 350 chrysanthemum plants cultivated in Yesan-gun, Chungcheongnam-do. Two viruses, chrysanthemum virus B (CVB) and tomato aspermy virus (TAV), and two viroids, chrysanthemum chlorotic mottle viroid (CChMVd) and chrysanthemum stunt viroid (CSVd), were detected in this study. The two viruses were detected in six samples and one sample, respectively. The two viroids were detected in 97 samples and 21 samples, respectively. The nucleotide sequences of the CVB-CN-Y, TAV-CN-Y, CChMVd-CN-Y, and CSVd-CN-Y obtained in this study showed 83.7–86.9%, 99.2–100.0%, 94.4–99.5%, and 95.7–99.7% identity, respectively, compared to their other strains/isolates. The CVB-CN-Y and TAV-CN-Y showed the greatest nucleotide sequence homology to CVB-GS1 and three TAV isolates (TAV-V, TAV-P, and TAV-ChJ), respectively. The CChMVd-CN-Y and CSVd-CN-Y showed the greatest nucleotide sequence homology to CChMVd-Horst and four CSVd isolates (Au1.1, K4pop, Sagae, and Tochigi), respectively. This study is the report on the infection rate of viruses and viroids in chrysanthemum plants cultivated in Yesan-gun in 2021.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43885546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

First Report of Tomato Spotted Wilt Virus in Oxypetalum coeruleum in Korea 韩国番茄斑点枯萎病首例报道

Research in Plant Disease Pub Date : 2022-12-31 DOI: 10.5423/rpd.2022.28.4.231

E. Baek, P. Palukaitis, J. Yoon

{"title":"First Report of Tomato Spotted Wilt Virus in Oxypetalum coeruleum in Korea","authors":"E. Baek, P. Palukaitis, J. Yoon","doi":"10.5423/rpd.2022.28.4.231","DOIUrl":"https://doi.org/10.5423/rpd.2022.28.4.231","url":null,"abstract":"Oxypetalum coeruleum, commonly known as Tweedia, is a perennial herbaceous plant of the Apocynaceae family native to southern Brazil and Uruguay. Tweedia plants are grown as one of the most popular ornamental flowers for floral arrangement in Korea. In May 2021, several tweedia plants in a single greenhouse in Gimje, Jeollabuk-do were found to show virus-like symptoms including necrotic rings, vein-clearing, chlorotic mottle, and mosaic on the leaves, and necrosis on the stems. Here, we have identified tomato spotted wilt virus (TSWV) in symptomatic tweedia leaves by applying high-throughput RNA sequencing. In the result, a single infection by TSWV was verified without mixed infections of different virus species. To confirm the presence of TSWV, a reverse transcription polymerase chain reaction was performed with a specific primer set to the N gene of TSWV. The complete genomic sequence of L, M, and S segments of TSWV ’Oxy’ isolate were determined and deposited in GenBank under accession numbers LC671525, LC671638, and LC671639, respectively. In the phylogenetic tree analysis by maximum likelihood method, ’Oxy’ isolate showed a high relationship with TSWV ’Gumi’ isolate from Gerbera jamesonii in Gyeongsangbuk-do, Korea; for all three RNA segments. To our knowledge, this is the first report of TSWV infection of O. coeruleum in Korea.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47799333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Occurence of Powdery Mildew Caused by Podosphaera xanthii on Vigna nakashimae in Korea 韩国黄氏Podosphaera在Vigna nakashimae上的发生

Research in Plant Disease Pub Date : 2022-12-31 DOI: 10.5423/rpd.2022.28.4.248

Kyung-Gu Min, Tae-Min Park, Y. Park, M. Jang

引用次数: 0

Efficacy of Hydrogen Peroxide on Root Rot Disease of Ginseng Sprouts 过氧化氢对人参芽根腐病的防治作用

Research in Plant Disease Pub Date : 2022-12-31 DOI: 10.5423/rpd.2022.28.4.204

Jong-Seok Song, G. Ahn, S. Jung

引用次数: 1

Phylogeny, Morphology and Pathogenicity of Biscogniauxia mediterranea Causing Charcoal Canker Disease on Quercus brantii in Southern Iran 伊朗南部栎炭化溃疡病的地中海褐裂菌的系统发育、形态和致病性

Research in Plant Disease Pub Date : 2022-12-31 DOI: 10.5423/rpd.2022.28.4.209

S. Ahmadi, Fariba Ghaderi, H. Charehgani, S. Karami, D. Safaee

{"title":"Phylogeny, Morphology and Pathogenicity of Biscogniauxia mediterranea Causing Charcoal Canker Disease on Quercus brantii in Southern Iran","authors":"S. Ahmadi, Fariba Ghaderi, H. Charehgani, S. Karami, D. Safaee","doi":"10.5423/rpd.2022.28.4.209","DOIUrl":"https://doi.org/10.5423/rpd.2022.28.4.209","url":null,"abstract":"Charcoal canker of oak, which has recently increased in southern Iran, could pose a serious threat to the entire forest ecosystem in the near future. In addition, it seems that climate change and its consequences, such as drought in the southern regions of Iran, have exacerbated this phenomenon. Consequently, the objective of this study was to identify the fungal pathogens that could cause charcoal canker disease in the oak forests of South Zagros. It was also sought to find associations between changes in the occurrence/exacerbation of charcoal canker disease under non and intense drought stress in non-inoculated or inoculated Quercus brantii seedlings. In total, 120 isolates were obtained from eight oak forests located in the Zagros Mountains of Southern Iran, Kohgiluyeh & Boyer-Ahmad and Fars provinces, which were classified as Biscogniauxia mediterranea based on morphological assessment. Subsequently, molecular assay confirmed the result by phylogenetic inference of internal transcribed spacer–rDNA regions, α-actin, and β-tubulin genes. The results of the pathogenicity test showed that the response of isolates of B. mediterranea (Iran-G1 and Iran-M70) was varied in different environments for the measured necrotic lesion length. In comparison with the control moisture treatments (non-stress), the necrotic lesion length in inoculated treatments increased under intense drought stress. In general, inoculated oak seedlings’ exposure to water-deficient stress by the pathogen of B. mediterranea could affect the spread/severity of the charcoal canker disease.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42375463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Damping-off of Edible Aster Caused by Rhizoctonia solani AG-4 茄枯丝核菌AG-4致食用紫菀萎蔫

Research in Plant Disease Pub Date : 2022-12-31 DOI: 10.5423/rpd.2022.28.4.245

Youn-Gi Moon, Se-won Kim, K. Park, Wan-Gyu Kim

{"title":"Damping-off of Edible Aster Caused by Rhizoctonia solani AG-4","authors":"Youn-Gi Moon, Se-won Kim, K. Park, Wan-Gyu Kim","doi":"10.5423/rpd.2022.28.4.245","DOIUrl":"https://doi.org/10.5423/rpd.2022.28.4.245","url":null,"abstract":"In August 2021, we surveyed diseases of wild vegetables grown in Taebaek, Gangwon Province, Korea. During the disease survey, we observed severe damping-off symptoms in young edible aster (Aster scaber) plants in a vinyl greenhouse investigated. The incidence of the disease in the plants ranged from 5% to 20%. Diseased plants of edible aster were collected from the vinyl greenhouse, and fungi were isolated from petiole lesions of the diseased plants. Rhizoctonia sp. was consistently isolated from the petiole lesions. We examined morphological characteristics and anastomosis groups of nine Rhizoctonia sp. isolates obtained from the petiole lesions. The examination results revealed that all the isolates corresponded to Rhizoctonia solani AG-4 based on the morphological characteristics and anastomosis test. Three isolates of R. solani AG-4 were tested for their pathogenicity on edible aster plants by artificial inoculation. Inoculation tests showed that the tested isolates caused damping-off symptoms on the inoculated plants. The induced symptoms were similar to those observed in the vinyl greenhouse investigated. Damping-off of edible aster caused by R. solani AG-4 is first reported in this study.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47420791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring the Reoccurrence of Fire Blight and the Eradication Efficiency of Erwinia amylovora in Burial Sites of Infected Host Plants Using Sentinel Plants 利用哨兵植物监测寄主植物埋葬区火斑病的再次发生和淀粉型欧文氏菌的根除效率

Research in Plant Disease Pub Date : 2022-12-31 DOI: 10.5423/rpd.2022.28.4.221

In Woong Park, Yu-Rim Song, Nguyen Trung Vu, Eom-Ji Oh, I. Hwang, H. Ham, Seong Hwan Kim, D. Park, C. Oh

{"title":"Monitoring the Reoccurrence of Fire Blight and the Eradication Efficiency of Erwinia amylovora in Burial Sites of Infected Host Plants Using Sentinel Plants","authors":"In Woong Park, Yu-Rim Song, Nguyen Trung Vu, Eom-Ji Oh, I. Hwang, H. Ham, Seong Hwan Kim, D. Park, C. Oh","doi":"10.5423/rpd.2022.28.4.221","DOIUrl":"https://doi.org/10.5423/rpd.2022.28.4.221","url":null,"abstract":"The fire blight caused by Erwinia amylovora (Ea) was first reported in 2015 in Korea, and the disease has rapidly spread to 22 regions until 2021. In Korea, all host plants in the apple and pear orchards where fire blight occurred should be eliminated and buried by the Plant Protection Act. To prevent the spread of the disease, all burial sites were prohibited from planting the new host plants for the next three years. To confirm the eradication efficiency of Ea and the reoccurrence of fire blight, the surveillance facilities were established on three burial sites from 2019 to 2020 in Anseong-si, Gyeonggi-do, and Chungju-si, Chungcheongbuk-do. As host plants, five apple trees of fire blight-susceptible cultivar ‘Fuji’, were planted in each facility. All facilities were enclosed with fences and nets and equipped with two CCTVs, motion sensors, and several other sensors for recording weather conditions to monitor the environment of the sentinel plants in real-time. The sentinel plants were checked for the reoccurrence of fire blight routinely. Suspicious plant parts were collected and analyzed for Ea detection by loop-mediated isothermal amplification polymerase chain reaction and conventional polymerase chain reaction. Until November 2022, Ea has not been detected in all sentinel plants. These results might support that the burial control of infected plants in soil works efficiently to remove Ea and support the possibility to shorten the prohibition period of host plant establishment in the burial sites.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43552842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Survey and Identification of Didymellaceae Causing Stem Canker Disease of Eucalyptus (E. camaldulensis) in Ethiopia 埃塞俄比亚桉树茎溃疡病病原菌Didymellaceae的调查与鉴定

Research in Plant Disease Pub Date : 2022-09-30 DOI: 10.5423/rpd.2022.28.3.132

Wendu Admasu, A. Sintayehu, A. Gezahgne

{"title":"Survey and Identification of Didymellaceae Causing Stem Canker Disease of Eucalyptus (E. camaldulensis) in Ethiopia","authors":"Wendu Admasu, A. Sintayehu, A. Gezahgne","doi":"10.5423/rpd.2022.28.3.132","DOIUrl":"https://doi.org/10.5423/rpd.2022.28.3.132","url":null,"abstract":"Plantation forests are established by planting Eucalyptus tree species to provide timber and pulp for the construction industries and to meet the energy needs in Ethiopia. Besides the extensive Eucalyptus plantations in the country, fungal pathogen-related diseases are the main challenges to successful production and management. The disease survey was conducted in the Eucalyptus growing areas of Ethiopia during 2019/2020 and 2020/2021. The objective of this research was to assess the diseases associated with Eucalyptus plant species and identify the causal fungal species. Plants of E. camaldulensis were the dominant species in the survey fields and were severely associated with stem and branch canker diseases. Diseased samples were collected and fungal species were identified as Didymellaceae according to culture morphology and affirmed by internal transcribed spacer sequence analysis. In phylogeny, isolates in the study and a reference strain formed supportive monophyletic clades with strong 90% and 95% support with Didymella coffeae-arabicae and Didymella pinodella respectively. Pathogenicity tests revealed that Didymellaceae could infect E. camaldulensis. The findings are the first reports of Eucalyptus stem canker disease caused by Didymellaceae in Ethiopia.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49052434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of Bacterial Antagonists to Develop an Effective Cocktail against Erwinia amylovora 筛选细菌拮抗剂研制抗淀粉性欧文氏菌有效鸡尾酒

Research in Plant Disease Pub Date : 2022-09-30 DOI: 10.5423/rpd.2022.28.3.152

D. Choi, Hyunchul Choi, Yeon Ju Kim, Yeon-jeong Lim, Ingyeong Lee, D. Park

{"title":"Screening of Bacterial Antagonists to Develop an Effective Cocktail against Erwinia amylovora","authors":"D. Choi, Hyunchul Choi, Yeon Ju Kim, Yeon-jeong Lim, Ingyeong Lee, D. Park","doi":"10.5423/rpd.2022.28.3.152","DOIUrl":"https://doi.org/10.5423/rpd.2022.28.3.152","url":null,"abstract":"Several types of chemical bactericides have been used to control fire blight. However, their excessive usage leads to environmental deterioration. Therefore, several researchers have analyzed antagonistic microorganisms as promising, effective, and safe biological control agents (BCAs). The primary aim of this study was to screen for potential antagonistic bacteria that suppress Erwinia amylovora. Among the 45 isolates studied, 5 strains showed the largest inhibition zone against E. amylovora. 16S rRNA gene sequencing identified them as Bacillus amyloliquefaciens (KPB 15), B. stratosphericus (KPB 21), B. altitudinis (KPB 25), B. safensis (KPB 31), and B. subtilis (KPB 39). KPB 25 and 31 reduced the lesion size of fire blight by 50% in immature apple fruits, and did not show antagonism against each other. Therefore, KPB 25 and 31 were selected to develop an antagonistic mixture against fire blight. Although the mixture with KPB 25 and 31 showed a slightly increased ability to reduce lesion size on immature fruits, they did not exhibit a synergistic effect in reducing E. amylovora population compared to each strain alone. Nevertheless, we have identified these two strains as useful and novel BCAs against fire blight with additional benefits safety and potential in developing a mixture without loss of their activity, owing to the absence of antagonism against each other.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43708815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

First Report of Pectobacterium brasiliense Causing Soft Rot on Graft Cactus in Korea 巴西乳杆菌引起移植仙人掌软腐病在韩国的首次报道

Research in Plant Disease Pub Date : 2022-09-30 DOI: 10.5423/rpd.2022.28.3.172

Kyoung-Taek Park, Soo-Min Hong, C. Back, S. Kim, Seung-Yeol Lee, I. Kang, L. Ten, H. Jung

{"title":"First Report of Pectobacterium brasiliense Causing Soft Rot on Graft Cactus in Korea","authors":"Kyoung-Taek Park, Soo-Min Hong, C. Back, S. Kim, Seung-Yeol Lee, I. Kang, L. Ten, H. Jung","doi":"10.5423/rpd.2022.28.3.172","DOIUrl":"https://doi.org/10.5423/rpd.2022.28.3.172","url":null,"abstract":"The graft cactus (Gymnocalycium mihanovichii) continues to be exported to more than 20 countries worldwide. In April 2021, typical bacterial symptoms of soft rot were observed in the graft cactus (cv. Yeonbit) in Goyang, Gyeonggi-do, Korea, resulting in economic losses in cactus production. The stems turned dark brown and the flowers were covered with black rot. The bacterial strain designated as KNUB-01-21 was isolated from infected stems and flowers. The results of the morphological and biochemical tests of the isolate were similar to those of Pectobacterium brasiliense. For molecular analysis, the 16S rRNA region and three housekeeping genes (dnaX, leuS, and recA) of the strain KNUB-01-21 were amplified. Based on the results of the molecular analysis and morphological and biochemical tests, KNUB-01-21 was identified as P. brasiliense. The pathogenicity of KNUB-01-21 on graft cactus was confirmed by an inoculation test. Artificial inoculation using P. brasiliense KNUB-01-21 produced soft rot symptoms on the grafted cactus, and the same bacterium was re-isolated and re-identified. This is the first report of P. brasiliense causing soft rot in graft cactus in Korea.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42952888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4