Research in Plant Disease最新文献_第6页

Establishment of Pathogenicity Test Method for Macrophomina phaseolina Causing Soybean Charcoal Rot 大豆炭腐病菌致病性试验方法的建立

Research in Plant Disease Pub Date : 2023-03-31 DOI: 10.5423/rpd.2023.29.1.1

Soukan An, Heungtae Kim

{"title":"Establishment of Pathogenicity Test Method for Macrophomina phaseolina Causing Soybean Charcoal Rot","authors":"Soukan An, Heungtae Kim","doi":"10.5423/rpd.2023.29.1.1","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.1.1","url":null,"abstract":"The establishment of a laboratory assay and a greenhouse assay was conducted for evaluating the pathogenicity of Macrophomina phaseolina causing soybean charcoal rot established. In the laboratory assay, microsclerotia and hyphae were used as inoculum. In the laboratory assays using microsclerotia as an inoculum, disease incidences of M. phaseolina NSW17-108 and HSM17-034 were higher at 35°C than 25°C. Of the two isolates NSW17-108 and HSM17-034, the disease incidence of HSM17-034 isolated from diseased sesame is higher than that of NSW17-108 isolated from diseased soybean. When the mycelia of M. phaseolina were used as an inoculum, the disease incidence of NSW17-108 and HSM17-034 at 35°C exceeded 80% even after only 5 days of inoculation. Even at 25°C, furthermore, that of HSM17-034 exceeded 80% 5 days later. In the pathogenicity assays at a greenhouse, toothpicks where microsclerotia were produced or microsclerotia harvested from potato dextrose agar medium were used as an inoculum. In all greenhouse assays, M. phaseolina NSW17-108 and HSM17-034 showed 40–60% of disease incidences 35–65 days after inoculation with the pathogen, depending on the inoculation method. Between the two isolates, the pathogenicity of HSM17-034 was stronger than that of NSW17-108, and this result was consistent with laboratory assay results. Since the laboratory and greenhouse test methods tested in this study have different advantages and disadvantages depending on each test method, it is thought that the test method that can meet the purpose of the study should be selected and used.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44278854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Multiplex PCR for Simultaneous Detection of Blueberry Red Ringspot Virus and Blueberry Scorch Virus Including an Internal Control 同时检测蓝莓红环斑病毒和蓝莓焦枯病毒的多重PCR方法的建立

Research in Plant Disease Pub Date : 2023-03-31 DOI: 10.5423/rpd.2023.29.1.94

H. Lee, E. Song, K. Ryu

{"title":"Development of a Multiplex PCR for Simultaneous Detection of Blueberry Red Ringspot Virus and Blueberry Scorch Virus Including an Internal Control","authors":"H. Lee, E. Song, K. Ryu","doi":"10.5423/rpd.2023.29.1.94","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.1.94","url":null,"abstract":"Blueberry red ringspot virus (BRRSV) and blueberry scorch virus (BlScV) are included in the quarantine virus list managed by the Korean Animal and Plant Quarantine Agency. A multiplex polymerase chain reaction (PCR) assay with an internal control was developed for the simultaneous detection of both viruses. The specific primers used here were designed based on the highly conserved regions of the genomic sequences of each virus, obtained from the National Center for Biotechnology Information nucleotide databases. The primers were designed to amplify a partial sequence within coat protein (CP) for detecting BRRSV and a partial sequence within the CP-16 kDa for detecting BlScV. 18S ribosomal RNA (rRNA) was used as internal control, and the primer set used in a previous study was modified in this study for detecting 18S rRNA. Each conventional PCR using the BRRSV, BlScV, and 18S rRNA primers exhibited a sensitivity of approximately 1 fg plasmid DNA. The multiplex PCR assay using the BRRSV, BlScV, and 18S rRNA primers was effective in simultaneously detecting the two viruses and 18S rRNA with a sensitivity of 1 fg plasmid DNA, similar to that of conventional PCR assays. The multiplex PCR assay developed in this study was performed using 14 blueberry cultivars grown in South Korea. BRRSV and BlScV were not detected, but 18S rRNA was all detected in all the plants tested. Therefore, our optimized multiplex PCR assay could simultaneously detect the two viruses and 18S rRNA in field samples collected from South Korea in a time-efficient manner. This approach could be valuable in crop protection and plant quarantine management.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43372113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence and Distribution of Root-Knot Nematodes in Kiwifruit Orchard 猕猴桃果园根结线虫的发生与分布

Research in Plant Disease Pub Date : 2023-03-31 DOI: 10.5423/rpd.2023.29.1.45

Heonil Kang, Hwanseok Je, In-Sil Choi

{"title":"Occurrence and Distribution of Root-Knot Nematodes in Kiwifruit Orchard","authors":"Heonil Kang, Hwanseok Je, In-Sil Choi","doi":"10.5423/rpd.2023.29.1.45","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.1.45","url":null,"abstract":"The study was conducted to investigate the infestation and distribution of plant-parasitic nematodes on kiwi orchards in Korea. Plant parasitic nematodes genus and densities were investigated at a total of 102 sites in Jeollanam-do, Gyeongsangnam-do, and Jeju-do, which are the main production areas of domestic kiwi orchards. Plant parasitic nematodes detected were of 9 genera, including root-knot nematodes (Meloidogyne spp.), spiral nematodes (Helicotylenchus spp.), and needle nematodes (Paratylenchus spp.), and 56% of the 102 plantations were infected with root-knot nematodes. Root-knot nematodes were found to be the most important plant parasitic nematode in domestic kiwi orchards. The average density of root-knot nematodes is 97 per 300 cm3 soil, and there is concern about the kiwi yield reduction. As a result of identifying the root-knot nematode species: M. arenaria, M. hapla, M. incognita, and M. javanica. Among them, M. arenaria is the most dominant. As the plant parasitic nematode infection route in fruit trees is often spread through the transplantation of infected seedlings, attention should be paid to the production of nematode-free plants during the production and supply of kiwifruit plants.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46528632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Report of Fire Blight Caused by Erwinia amylovora on Korean Mountain Ash (Sorbus alnifolia) in Korea 韩国山梨花楸(Sorbus alnifolia)上淀粉状Erwinia amylovora引起的火疫病首次报道

Research in Plant Disease Pub Date : 2023-03-31 DOI: 10.5423/rpd.2023.29.1.79

Yeon-Jeong Lim, Hyeonseok Oh, Mi-Hyun Lee, Eunjung Roh, Hyeonheui Ham, Dong Suk Park, Duck Hwan Park, Yong Hwan Lee

引用次数: 1

Pectobacterium brasiliense as a Causative Agent for Soft Rot of Radish in Korea 韩国萝卜软腐病病原菌巴西乳杆菌的研究

Research in Plant Disease Pub Date : 2023-03-31 DOI: 10.5423/rpd.2023.29.1.64

Kyoung-Taek Park, Soo-Min Hong, C. Back, I. Kang, Seung-Yeol Lee, L. Ten, H. Jung

引用次数: 2

First Report of Fire Blight Caused by Erwinia amylovora on Korean Mountain Ash (Sorbus alnifolia) in Korea 韩国山梨花楸(Sorbus alnifolia)上淀粉状Erwinia amylovora引起的火疫病首次报道

Research in Plant Disease Pub Date : 2023-03-31 DOI: 10.5423/rpd.2022.28.4.79

Yeon-jeong Lim, H. Oh, Mi-Hyun Lee, Eunjung Roh, H. Ham, D-S Park, D. Park, Yong Hwan Lee

引用次数: 0

First Report of Leaf Spot in Fischer’s Ragwort Caused by Didymella ligulariae 由舌形Didymella ligulariae引起的费雪舌草叶斑病初报

Research in Plant Disease Pub Date : 2023-03-31 DOI: 10.5423/rpd.2023.29.1.60

Gyo-Bin Lee, H. Shim, W. Cho, Wan-Gyu Kim

引用次数: 0

Incidence of Virus Diseases in Major Cultivated Areas of Watermelon and Melon in Chungbuk Province 重庆省西瓜甜瓜主产区病毒病发病率

Research in Plant Disease Pub Date : 2023-03-31 DOI: 10.5423/rpd.2023.29.1.88

Jong-Woo Han, Y. Park, C. Youn, S. Lee, Taek-Goo Jeong, Hongsoo Choi, Mi-Kyeong Kim

{"title":"Incidence of Virus Diseases in Major Cultivated Areas of Watermelon and Melon in Chungbuk Province","authors":"Jong-Woo Han, Y. Park, C. Youn, S. Lee, Taek-Goo Jeong, Hongsoo Choi, Mi-Kyeong Kim","doi":"10.5423/rpd.2023.29.1.88","DOIUrl":"https://doi.org/10.5423/rpd.2023.29.1.88","url":null,"abstract":"To investigate the incidence status of viruses in major cultivated areas of watermelon and melon in Chungbuk Province, samples were collected from 2020 to 2021 in vinyl greenhouse of Jincheon and Eumseong and examined for virus infection using reverse transcription polymerase chain reaction. Of the six viruses on watermelon that was analyzed in this study, watermelons were infected with cucumber mosaic virus (CMV), watermelon mosaic virus (WMV), cucumber green mottle mosaic virus (CGMMV), and cucurbit aphid-borne yellows virus (CABYV). The incidence rate of CMV was 20.9–35.0%, WMV 0.4–15.8%, CGMMV 1.6–38.5%, and CABYV was 3.5–3.7% from 2020 to 2021. But strangely, there were no incidence of zucchini yellow mosaic virus and cucurbit chlorotic yellows virus (CCYV) during investigation. From this result, we knew the major virus was CGMMV on watermelon in Chungbuk Province. Molecular diagnosis assays of the two melon viruses, showed that melons were infected with CABYV and CCYV from 2020 to 2021. The incidence rate of CABYV was 53.9–92.2% and CCYV was 2.7–20.8%. The incidence of CABYV was high in melon cultivation of Jincheon and Eumseong, Chungbuk. Afterwards, it is necessary to establish a control management strategy for reduce the incidence of CABYV. Furthermore, we must pay attention that of CCYV even if the incidence was low.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49231221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trends in Mycotoxin Contamination of Cereals and Cereal Products in Korea 韩国谷物及谷物制品霉菌毒素污染趋势

Research in Plant Disease Pub Date : 2022-12-31 DOI: 10.5423/rpd.2022.28.4.179

Theresa Lee, S. Baek, Sosoo Kim, Ji-Seon Paek, J. Park, Jangnam Choi, Jungsun Choi, J. Jang, Jeomsoon Kim

{"title":"Trends in Mycotoxin Contamination of Cereals and Cereal Products in Korea","authors":"Theresa Lee, S. Baek, Sosoo Kim, Ji-Seon Paek, J. Park, Jangnam Choi, Jungsun Choi, J. Jang, Jeomsoon Kim","doi":"10.5423/rpd.2022.28.4.179","DOIUrl":"https://doi.org/10.5423/rpd.2022.28.4.179","url":null,"abstract":"In this review, the mycotoxin contamination of Korean cereals and their products is analyzed by crop based on scientific publications since 2000. Barley, rice, and corn were investigated heavier than the others. The common mycotoxins occurred in all cereals and their products were deoxynivalenol and zearalenone. Nivalenol was detected in all samples analyzed but more frequently or mainly in barley, rice, and oat. Fumonisin was commonly detected in corn and sorghum but also in adlay, millet, and rice. Adlay and millet were similar in the contamination pattern that fumonisin and zearalenone were the most frequently detected mycotoxins. Zearalenone was the most commonly detected mycotoxin with concentrations higher than the national standards (maximum limit), followed by deoxynivalenol, and aflatoxin. However, most occurrence levels were below the maximum limits for respective mycotoxins. This result shows that barley, rice, corn, sorghum, millet, and adlay are more vulnerable to mycotoxin contamination than other cereals and therefore continuous monitoring and safety management are necessary.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42643815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Biocontrol of Maize Diseases by Microorganisms 微生物对玉米病害的生物防治

Research in Plant Disease Pub Date : 2022-12-31 DOI: 10.5423/rpd.2022.28.4.195

Jung-Ae Kim, Jeong-Sup Song, M. Jeong, Sook-Young Park, Yangseon Kim

引用次数: 0